Article
Multidisciplinary Sciences
Ikuko Nakajima, Hiroyuki Kawahigashi, Chikako Nishitani, Akifumi Azuma, Takashi Haji, Seiichi Toki, Masaki Endo
Summary: Transposition of transposable elements can affect the expression, splicing, epigenetic status, and function of genes located near the inserted/excised locus. In grape, the presence of the Gret1 retrotransposon in the promoter region of the VvMYBA1a allele suppresses the expression of the VvMYBA1 transcription factor gene, resulting in the green berry skin color of the 'Shine Muscat' grape cultivar in Japan. By targeting Gret1 with CRISPR/Cas9, we successfully eliminated the transposon in 19 out of 45 transgenic plants, demonstrating the potential for transposon removal through genome editing.
Article
Multidisciplinary Sciences
Weijian Huang, Meng Li, Huangwei Zhang, Juyou Wu, Jim M. Dunwell, Shaoling Zhang
Summary: By analyzing agronomic traits and sequencing data, the differences between cultivated and wild grapes and peaches were compared in terms of fruit size, flavor, resistance, and color. Cultivated varieties showed advantages in fruit size, sugar content, and sugar-acid ratio. Wild varieties had utility value in terms of resistance. The study identified differentially expressed genes in cultivated and wild grapes and peaches, with some common genes between them.
SCIENTIFIC REPORTS
(2023)
Article
Forestry
Ling Su, Meng Qi, Dong Meng, Qing Yang, Yongmei Wang, Fengshan Ren, Liying Yang, Yingchun Chen, Liyuan Liu, Meiling Tang, Yangbo Song, Lei Gong
Summary: In order to cultivate different grape varieties according to market needs, it is important to study the regulation mechanism of color changes in grapes at different development stages. This study used RNA-sequencing technology to compare and analyze the transcriptome data of four grape varieties at the same development stage. The anthocyanin synthesis pathway in the flavonoid pathway was mainly studied among the annotated differential genes. The role of the MYB transcription factor MYB2 in anthocyanin regulation was confirmed through subcellular localization and gene function verification.
Article
Biochemistry & Molecular Biology
Chen Chen, Hong Chen, Chao Han, Zemao Liu, Fangyuan Yu, Qikui Wu
Summary: This study investigated the effect of 24-epibrassinolide (EBL) on the concentration and composition of fatty acids (FA) in Styrax tonkinensis seed oil. Transcriptome sequencing and GC-MS were used to identify candidate genes involved in FA biosynthesis. The results showed that EBL5 treatment increased FA concentration and enhanced enzyme activity by promoting the expression of FA biosynthesis-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Linnan Wu, Pengcheng Li, Haifeng Jia, Fesobi Olumide Phillip, Xingcheng Bao, Fengyun Zhao, Benzhou Zhao, Jianrong Feng, Kun Yu
Summary: The study demonstrated that foliar application of KH2PO4 could increase total soluble solids, flavonoid, and total anthocyanin contents in grape skin. Additionally, T2 treatment resulted in lower acidity and higher pH in grape juice, as well as darker skin color compared to other treatments.
Article
Biochemistry & Molecular Biology
Han Zhang, Jiangyu Wang, Shuting Tian, Wenhui Hao, Lingjuan Du
Summary: Floral colour is an important agronomic trait for ornamental plants. This study investigated the regulatory mechanism of light-induced anthocyanin biosynthesis in grape hyacinth. The two B-box proteins, MaBBX20 and MaBBX51, were found to be associated with light-induced anthocyanin biosynthesis. MaBBX20 was shown to positively regulate anthocyanin biosynthesis, while MaBBX51 acted as a negative regulator. The study provides valuable insights into the molecular mechanisms of flower colouration in ornamental plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Horticulture
You-Mei Li, Xiao-Rui Sun, Jia-Ling You, Xiu-Jie Li, Bo Li, Zhao-Sen Xie
Summary: ABA applications accelerate grape ripening and improve quality attributes through modulation of gene expression related to flavonoid biosynthesis, sucrose metabolism, and sugar transport, leading to increased anthocyanin and sugar accumulation.
SCIENTIA HORTICULTURAE
(2022)
Article
Biotechnology & Applied Microbiology
Songtao Jiu, Le Guan, Xiangpeng Leng, Kekun Zhang, Muhammad Salman Haider, Xiang Yu, Xudong Zhu, Ting Zheng, Mengqing Ge, Chen Wang, Haifeng Jia, Lingfei Shangguan, Caixi Zhang, Xiaoping Tang, Muhammad Abdullah, Hafiz Umer Javed, Jian Han, Zhigang Dong, Jinggui Fang
Summary: This study elucidates the roles of VvMYBA2r and VvMYBA2w alleles in grape skin coloration, with VvMYBA2r positively regulating anthocyanin biosynthesis while VvMYBA2w showing limited efficacy. Different MYB haplotypes and ploidy levels also influence the grape skin color.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Nutrition & Dietetics
Peian Zhang, Suwen Lu, Zhongjie Liu, Ting Zheng, Tianyu Dong, Huanchun Jin, Haifeng Jia, Jingggui Fang
Summary: The study showed that different light qualities have varied effects on grape berry quality, with blue light increasing anthocyanins and sugars, and reducing organic acids, while red light had the opposite effect. Transcriptomics and metabolomics analyses identified differentially expressed genes and significantly changed metabolites related to photosynthesis and flavonoid biosynthesis. Weighted gene co-expression network analysis revealed modules associated with anthocyanins and soluble sugars. These findings provide insights into using supplemental light to improve fruit quality.
FRONTIERS IN NUTRITION
(2021)
Article
Horticulture
Lijin Lin, ZhiYu Li, Jin Wang, Dong Liang, Hui Xia, Xiulan Lv, Yi Tang, Xun Wang, Qunxian Deng, Ming'an Liao
Summary: The effects of different concentrations of 24-epibrassinolide on selenium stress and accumulation in grapevine were evaluated. The results showed that 24-epibrassinolide can increase grapevine biomass, photosynthetic pigment content, and antioxidant enzyme activity. It also promotes selenium uptake and organic selenium transport in grapevine.
SCIENTIA HORTICULTURAE
(2023)
Article
Multidisciplinary Sciences
Jiali Zhao, Hongyou Li, Juan Huang, Taoxiong Shi, Ziye Meng, Qingfu Chen, Jiao Deng
Summary: In this study, 28 Tartary buckwheat BBX genes were identified and analyzed in terms of their properties, structures, distribution, and phylogeny. The results showed that these genes have varying expression under different conditions and may play important roles in the growth and development of Tartary buckwheat.
Article
Agronomy
Linyuan Duan, Bo Zhang, Guoli Dai, Xinru He, Xuan Zhou, Ting Huang, Xiaojie Liang, Jianhua Zhao, Ken Qin
Summary: Wolfberry is a small shrub with medicinal and edible properties, mainly available in red, purple, and yellow phenotypes. It is rich in flavonoids, which contribute to the diverse colors of plants. This study analyzed the metabolite and gene expression profiles of different colored wolfberries and identified the molecular mechanism underlying their color differences. The accumulation of bluish anthocyanins was found in purple wolfberries, while red and yellow wolfberries accumulated more yellowish flavonoids. Additionally, differentially expressed genes related to flavonoid synthesis and transcription factors were identified among wolfberries of different colors. These findings provide new insights into the molecular mechanism of fruit color differences in wolfberry and offer new ideas for molecular breeding.
Article
Chemistry, Applied
Huixin Fang, Qian Zhou, Shunchang Cheng, Xin Zhou, Baodong Wei, Yingbo Zhao, Shujuan Ji
Summary: The study showed that treatment with 24-epibrassinolide (EBR) can effectively inhibit postharvest yellowing in broccoli by maintaining higher chlorophyll content and antioxidant enzyme activities, making it an effective method for improvement.
Article
Plant Sciences
Dongming Zhao, Yuxuan Zheng, Lingjun Yang, Ziyu Yao, Jianfeng Cheng, Fang Zhang, Haiyan Jiang, Dong Liu
Summary: The nuclear GARP transcription factor AtGLK1 was demonstrated to play a crucial role in regulating sucrose-induced anthocyanin biosynthesis in Arabidopsis. It functions by enhancing anthocyanin accumulation, participating in plastid-signaling mediated anthocyanin accumulations, and acting upstream of the key negative regulator MYBL2.
Review
Biochemistry & Molecular Biology
Zhongxin Liang, Hongrui Liang, Yizhan Guo, Dong Yang
Summary: Cyanidin 3-O-galactoside (Cy3Gal) is a widespread anthocyanin with positive health impacts, found in fruits like apples and berries. It shares a biosynthesis pathway and stability with other anthocyanins, but has unique biosynthesis involving galactosyltransferase using UDP-galactose and cyanidin. Chokeberry is identified as a practical source for mass-production. Its antioxidant properties and various health effects make it suitable for use as a food additive or health supplement, with confirmed physiological functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)