Article
Biochemistry & Molecular Biology
V. H. Giang Phan, Ramya Mathiyalagan, Minh-Thu Nguyen, Thanh-Tuyen Tran, Mohanapriya Murugesan, Tuyet-Nhung Ho, Ha Huong, Deok Chun Yang, Yi Li, Thavasyappan Thambi
Summary: Core-shell hydrogel beads for oral insulin delivery at the intestine were developed using alginate and chitosan polysaccharides. The beads demonstrated good biocompatibility and angiogenic response. The use of layered double hydroxides effectively prevented insulin leakage and the beads sustained release of insulin in simulated intestinal fluid.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Pharmacology & Pharmacy
Shafi Ullah, Asif Nawaz, Arshad Farid, Muhammad Shahid Latif, Muhammad Fareed, Shakira Ghazanfar, Charis M. Galanakis, Abdulhakeem S. Alamri, Majid Alhomrani, Syed Mohammed Basheeruddin Asdaq
Summary: The study aimed to embed folate-modified chitosan 5FU nanoparticles into calcium alginate beads for colon-targeted delivery. The results showed that the swelling, erosion, and water uptake properties of FA-CS-5FU-NP-Bf beads were significantly higher than those of FA-CS-5FU-NP-Bo beads at simulated intestinal pH.
Article
Pharmacology & Pharmacy
Ya-Wen Zhang, Ling-Lan Tu, Zhan Tang, Qiao Wang, Gao-Li Zheng, Li-Na Yin
Summary: The use of chitosan-conjugated deoxycholic acid/sodium alginate nanoparticles loaded with insulin can improve intestinal permeability and enhance insulin absorption, leading to a significant hypoglycemic effect in diabetic rat models.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Article
Chemistry, Physical
Kyung Hee Park, Yeon Woo Choi, Heejoo Ryu, Hyoung Jae Lee, Jae-Hak Moon, Ho-Jun Song, Yeong-Joon Park
Summary: This study developed improved multi-component beads for controlled and sustained delivery of antibiotics. Single and multi-component beads were prepared using chitosan, calcium chloride, alginate, and citric acid, and the release of antibiotics was controlled by crosslinking. The crosslinked beads showed efficient sustained-release of gentamicin.
Article
Pharmacology & Pharmacy
Li Shangyong, Zhang Hui, Chen Kaiwei, Jin Mengfei, Vu Son Hai, Samil Jung, He Ningning, Zheng Zhou, Myeong-Sok Lee
Summary: Oral drug delivery systems have advantages of simplicity and minimal side effects, making them suitable for colon-targeted therapy. Chitosan/alginate nanoparticles (CANPs) show potential in the treatment of intestinal diseases due to their strong electrostatic interaction and intestinal mucosal adhesion.
Article
Green & Sustainable Science & Technology
Huiping Zeng, Siqi Sun, Ke Xu, Weihua Zhao, Ruixia Hao, Jie Zhang, Dong Li
Summary: Magnetic alginate-chitosan porous beads based on iron (Fe) sludge (M-ACFBs) were prepared and showed high adsorption efficiency for As(V) removal from water. The ratio of magnetic nanoparticles to iron sludge, pH, contact time, temperature, and coexisting ions were found to affect the adsorption effect. The adsorption mechanism involves ligand exchange and electrostatic attraction, and the maximum adsorption capacity is 14.2 +/- 0.4 mg/g.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Chemistry, Applied
Feuangthit Niyamissara Sorasitthiyanukarn, Chawanphat Muangnoi, Pornchai Rojsitthisak, Pranee Rojsitthisak
Summary: In this study, ATX-loaded chitosan oligosaccharide/alginate nanoparticles (ATX-COANPs) were successfully fabricated with optimized conditions. These nanoparticles exhibited good stability and sustained release characteristics under various conditions. Encapsulation within COANPs led to increased in vitro bioaccessibility, bioavailability, and antioxidant activity of ATX, suggesting potential applications in developing an effective oral delivery system for nutraceuticals or functional foods.
FOOD HYDROCOLLOIDS
(2022)
Review
Biochemistry & Molecular Biology
Melbha Starlin Chellathurai, Chung Lip Yong, Zarif Mohamed Sofian, Shariza Sahudin, Najihah Binti Mohd Hasim, Syed Mahmood
Summary: Chitosan is a natural polysaccharide that is biodegradable, bioadhesive, and biocompatible. It has been extensively studied for oral insulin drug delivery systems. Electrostatic interaction between chitosan and insulin leads to the formation of self-assembled nanoparticles, which can protect insulin from degradation and improve intestinal uptake. Functionalized chitosan nanoparticles also offer opportunities for targeted and prolonged delivery. This review provides a comprehensive guide for formulating stable insulin-chitosan nanoparticles using mild ionic interactions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Multidisciplinary
Adelina-Gabriela Niculescu, Alexandru Mihai Grumezescu
Summary: Chitosan and alginate, two natural polymers, have attracted significant interest for their various applications in nano form. They have shown potential in biomedical, environmental protection, and food industry fields, serving as delivery vehicles, antimicrobial agents, and more. This paper discusses the latest applications of chitosan-alginate-based nanomaterials and serves as a starting point for further research.
Article
Chemistry, Applied
Feuangthit Niyamissara Sorasitthiyanukarn, Chawanphat Muangnoi, Pornchai Rojsitthisak, Pranee Rojsitthisak
Summary: CDD-CANPs, a nanoformulation developed using chitosan/alginate nanoparticles, exhibit good stability and sustained release profile, showing better cellular uptake and cytotoxicity against HepG2 cells.
CARBOHYDRATE POLYMERS
(2021)
Article
Pharmacology & Pharmacy
Peththa Wadu Dasuni Wasana, Hasriadi, Opa Vajragupta, Pornchai Rojsitthisak, Pasarapa Towiwat, Pranee Rojsitthisak
Summary: In this study, metformin and curcumin were co-encapsulated into chitosan/alginate nanoparticles using response surface methodology. The optimized nanoparticles displayed stability, sustained release of the drugs, enhanced mucoadhesion, and improved cellular uptake. Furthermore, the nanoparticles exhibited better anti-inflammatory effects and pain relief compared to the physical mixture of the drugs, without significant side effects in mice. This study establishes the efficacy and safety of a chitosan/alginate nanodelivery system for the combination of metformin and curcumin against pain.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Guan-Lan Chen, Hong-Ying Cai, Jian-Ping Chen, Rui Li, Sai-Yi Zhong, Xue-Jing Jia, Xiao-Fei Liu, Bing-Bing Song
Summary: Chitosan/alginate nanoparticles prepared by ionotropic pre-gelation can be used to encapsulate clam heparinoid DG1. The characteristics of the nanoparticles depend on the preparation parameters. Suitable conditions can produce DG1-NPS and DG1/Cur-NPS with small size, high encapsulation efficiency, high loading capacity, and negative zeta potential. In the murine model experiment, DG1-NPs enhanced the oral antithrombotic activity of DG1, while DG1/Cur-NPs did not show higher antithrombotic activity than DG1-NPs.
Article
Biochemistry & Molecular Biology
Edward Hermosilla, Marcela Diaz, Joelis Vera, Maria Jose Contreras, Karla Leal, Rodrigo Salazar, Leticia Barrientos, Gonzalo Tortella, Olga Rubilar
Summary: In this study, chitosan was used to encapsulate the mycelia of the white-rot fungus and form chitosan fungal beads for multiple extractions and nanoparticle synthesis. The resulting nanoparticles were composed of chitosan-silver nanoparticles with a size of 25 nm. The chitosan fungal beads lost their ability to produce chitosan-silver nanoparticles after three extractions and nanoparticle synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Aysun Aksu, Serap Cetinkaya, Ali Fazil Yeniduenya, Senay Akkus Cetinus, Hayreddin Gezegen, Burak Tuezuen
Summary: This study investigated the immobilization of pectinase on chitosan-alginate-clay beads using epichlorohydrin as a crosslinker. The immobilized enzyme showed improved hydrolytic properties, such as higher optimal temperature and pH. The immobilization process also allowed for reusability of the enzyme, with approximately 45% activity remaining after 5 cycles. Gaussian calculations and molecular docking were performed to further analyze the immobilization product and its effects in human metabolism.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biochemistry & Molecular Biology
Claudio P. Pinheiro, Loreane M. K. Moreira, Stephany S. Alves, Tito R. S. Cadaval Jr, Luiz A. A. Pinto
Summary: This study successfully extracted anthocyanins from Pinot Noir grape skins and concentrated them onto edible and safe chitosan and alginate beads through adsorption. The results showed that the adsorption performance and removal efficiency of chitosan beads were better than those of alginate beads under different pH conditions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Hassan Sheikh, Akbar John, Najiah Musa, Laith A. Abdulrazzak, Mulham Alfatama, Anis Fadhlina
Summary: This study systematically reviewed the feasibility of using Vibrio spp. and their vibriocins as a control measure for vibriosis in aquaculture. The results showed that Vibrio spp. can be used as a probiotic for vibriosis control, while vibriocins exhibited narrow-spectrum activity against Vibrio spp., making them a targeted control measure.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Review
Materials Science, Multidisciplinary
Yazid Zaiki, Lee Yong Lim, Tin Wui Wong
Summary: Key materials used in the design of oral insulin nanoparticles include intestinal receptor targeting, biomimetic virus mimicking, particulate surface charge switching, coat-core detachment, and combination approaches. Mucus penetration favors particles with hydrophilic/amphiphilic surfaces with no net charges, while mucosa penetration shows a preponderance for hydrophobic or positively charged surfaces.
INTERNATIONAL MATERIALS REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Ivan Lozada Lawag, Md Khairul Islam, Tomislav Sostaric, Lee Yong Lim, Katherine Hammer, Cornelia Locher
Summary: This study examined the phenolic content, antioxidant activity, and phenolic compounds in different honeys from Western Australia. The highest total phenolic content and antioxidant activity were found in Calothamnus spp. honey. Various phenolic compounds were identified and quantified in the honeys using High Performance Thin-Layer Chromatography (HTPLC). The identified compounds were found to contribute to the honeys' antioxidant activity, except for certain compounds like t-cinnamic acid and lumichrome.
Article
Biochemistry & Molecular Biology
Md Lokman Hossain, Lee Yong Lim, Katherine Hammer, Dhanushka Hettiarachchi, Cornelia Locher
Summary: This study reports on the release profile of MGO from five commercial products containing Manuka honey using Franz diffusion cell and HPLC analysis. The results showed that the release rate of MGO from an artificial honey matrix and NZ Manuka honey reached 99.49% and 98.05% respectively over a 12-hour period, while a time-dependent % MGO release between 85% and 97.18% was observed in the investigated formulations over the same period.
Article
Chemistry, Multidisciplinary
Ivan Lozada Lawag, Elisa S. Nolden, Arthur A. M. Schaper, Lee Yong Lim, Cornelia Locher
Summary: The Folin-Ciocalteu assay is commonly used for measuring total phenolic content (TPC) in honey, but it can be influenced by the presence of reducing sugars, resulting in overestimation of TPC. This study investigates the effect of pH on the assay and proposes a modified TPC method using appropriate pH conditions (pH 7.9) achieved with a 0.75% Na2CO3 solution to minimize interference from reducing sugars. The findings demonstrate the importance of pH control for accurate analysis of TPC in honey and validate the modified method according to ICH guidelines.
APPLIED SCIENCES-BASEL
(2023)
Article
Pharmacology & Pharmacy
Md Lokman Hossain, Lee Yong Lim, Katherine Hammer, Dhanushka Hettiarachchi, Cornelia Locher
Summary: This study presents the design, preparation, and physicochemical characterization of three types of alginate-based topical formulations incorporating honey. The developed manufacturing techniques yielded topical formulations with high honey content while preserving the integrity of the honey constituents, regardless of the honey type utilized. A storage stability study demonstrated that the monitored honey constituents retained their integrity after 6 months of storage.
Article
Chemistry, Medicinal
Batoul Alallam, Abd Almonem Doolaanea, Mulham Alfatama, Vuanghao Lim
Summary: Zinc oxide nanoparticles (Green-ZnO-NPs) were prepared using pure curcumin and showed additive antimicrobial and anticancer effects with curcumin. The physicochemical properties of Green-ZnO-NPs were characterised, and their inhibitory effect on bacterial strains and cancer cells was evaluated. The results demonstrated that Green-ZnO-NPs have potential as an alternative therapeutic agent for cancer treatment.
Review
Polymer Science
Batoul Alallam, Hazem Choukaife, Salma Seyam, Vuanghao Lim, Mulham Alfatama
Summary: Kidney disease is a significant global burden, and drug delivery to the kidneys is challenging. Nanotechnology offers potential solutions, as nanomaterials with unique properties have shown promising potential in renal therapy. Various types of nanosystems have been developed to improve drug delivery to the kidneys, and this review discusses their features and strategies.
Article
Chemistry, Analytical
Mariana Mello dos Santos, Christina Jacobs, Md Khairul Islam, Lee Yong Lim, Cornelia Locher
Summary: A novel HPTLC method using silica gel 60 F-254 plates with 1-butanol-2-propanol-aqueous boric acid solution as the mobile phase was developed for quantification of trehalulose in stingless bee honey. The method exhibited good linearity over the concentration range of 100-800 ng per band with a coefficient of correlation of 0.9996. The method was validated for various parameters and successfully applied for determination of trehalulose in stingless bee honey.
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
(2023)
Article
Chemistry, Multidisciplinary
Md Lokman Hossain, Lee Yong Lim, Katherine Hammer, Dhanushka Hettiarachchi, Cornelia Locher
Summary: This study presents data on the antioxidant and antibacterial activities of honey-based topical formulations incorporating four Western Australian (WA) honeys along with New Zealand Manuka honey as a comparator honey. The study found that the antioxidant activity was retained in all formulation types irrespective of the honey that was utilized. Additionally, the antibacterial activity of honey-loaded formulations was recorded to be comparable to that of their respective pure honeys.
APPLIED SCIENCES-BASEL
(2023)
Review
Pharmacology & Pharmacy
Okhee Yoo, Britta S. von Ungern-Sternberg, Lee Yong Lim
Summary: This review paper discusses the use of human taste panels and artificial neural networks (ANNs) in taste-masking paediatric drug formulations. Young adults (18-40) can serve as suitable substitutes for children due to their similar taste sensitivity. Taste panellists need not be experts in sensory evaluation, but should be screened for bitterness taste detection thresholds. ANNs can model complex relationships and enable predictions in taste-masking optimization. Continuous refinement of machine learning algorithms can enhance paediatric formulation development and overcome taste-masking challenges.
Article
Chemistry, Medicinal
Okhee Yoo, Sam Salman, Britta S. von Ungern-Sternberg, Lee Yong Lim
Summary: This study utilized the D-optimal mixture experimental design to determine the optimal component ratio of flucloxacillin, Eudragit EPO, and palmitic acid to prepare taste-masked microparticles. The interactions between the excipients and the drug influenced the stability and release of flucloxacillin. Storage temperature and humidity also affected the chemical stability of the microparticles, with over 90% of the drug load retained after 12 months under preferred storage conditions.
Article
Chemistry, Medicinal
Okhee Yoo, Sam Salman, Britta S. von Ungern-Sternberg, Lee Yong Lim
Summary: This paper presents a new taste-masked flucloxacillin ternary microparticle (FTM) formulation for children, which overcomes the challenges of bitter taste, quick dissolution, and large doses. A robust and reproducible fabrication method is developed, with potential scalability.
Article
Biochemical Research Methods
Md Lokman Hossain, Minh Nguyen, Leah Benington, Lee Yong Lim, Katherine Hammer, Dhanushka Hettiarachchi, Cornelia Locher
Summary: The aim of this study was to assess the release profile of components in five different honeys and their corresponding honey-loaded gel formulations using a custom-designed diffusion cell in combination with High-Performance Thin-Layer Chromatography (HPTLC). The newly developed approach coupled with HPTLC is conducive to monitor the release profile of minor honey constituents from pure honeys and honey-loaded semisolid formulations.
METHODS AND PROTOCOLS
(2023)
Article
Polymer Science
Ghufran Ullah, Asif Nawaz, Muhammad Shahid Latif, Kifayat Ullah Shah, Saeed Ahmad, Fatima Javed, Mulham Alfatama, Siti Aisyah Abd Ghafar, Vuanghao Lim
Summary: Bilayer/multilayer tablets are commonly used to tailor drug release, and this research aimed to formulate a novel gastro-retentive tablet to eliminate H. pylori in the gastrointestinal tract. Bilayer tablets were prepared using the direct compression technique, and the controlled-release bilayer tablets were prepared using hydrophilic swellable polymers (sodium alginate, chitosan, and HPMC-K15M). The study concluded that the combination of sodium alginate, chitosan, and HPMC polymers resulted in a gastro-retentive and controlled-release drug delivery system.