Article
Plant Sciences
Dong He, Rui Liang, Tuan Long, Ying Yang, Changyin Wu
Summary: Panicle abortion in rice is caused by defective spikelet development, with RBH1 encoding a pectate lyase protein essential for panicle formation and preventing panicle abortion.
Article
Food Science & Technology
Lieqin Shi, Qin Liu, Qinghua Qiao, Yulin Zhu, Wei Huang, Xiaomin Wang, Zhenxin Ren
Summary: The study investigated the effects of pectate and pectate lyase on the nutritional quality of tomato fruits postharvest. Differentiated expressed genes were found in amino acid metabolism, carbohydrate metabolism, and signal transduction pathways. The opposite effects of pectate and PL on fruit softening involve 56 genes with opposite expression trends.
Article
Biochemistry & Molecular Biology
Yuanxiu Lin, Hao He, Yanling Wen, Shuaipeng Cao, Zisen Wang, Ziqing Sun, Yunting Zhang, Yan Wang, Wen He, Mengyao Li, Qing Chen, Yong Zhang, Ya Luo, Xiaorong Wang, Haoru Tang
Summary: In this study, a comprehensive analysis of the FaPL gene family in the octoploid strawberry genome was conducted, and a total of 65 FaPL genes were identified. The study revealed that FaPL genes are mainly localized to the extracellular and cytoplasmic spaces. The expression levels of FaPL1, 3, 5, 20, 25, 42, and 57 gradually increased during strawberry fruit development and ripening, indicating their involvement in strawberry softening, with FaPL1 playing a vital role.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Xiaolong Huang, Guilian Sun, Zongmin Wu, Yu Jiang, Qiaohong Li, Yin Yi, Huiqing Yan
Summary: By genome-wide investigation, sixteen pectate lyase gene family members were identified in Fragaria vesca. Significant divergences were found among FvePLs in terms of gene duplication, cis-acting elements, and tissue expression patterns. FvePL6, 8, and 13 were determined to have significant expansions during evolution. FvePLs were closely correlated with transcription factors and genes involved in plant development, abiotic/biotic stresses, ions/Ca2+, and hormones.
Article
Biochemistry & Molecular Biology
Yuying Ma, Chukun Wang, Zhen Gao, Yuxin Yao, Hui Kang, Yuanpeng Du
Summary: The ripening and softening process in grape is associated with the depolymerization of pectin components, which is facilitated by pectin lyases (PLs). This study identified 16 VvPL genes in the grape genome, and found that VvPL5, VvPL9, and VvPL15 are highly expressed during grape ripening, indicating their involvement in grape softening. Overexpression of VvPL15 in Arabidopsis affected pectin content and plant growth, while antisense expression of VvPL15 confirmed the relationship between VvPL15 and pectin content. Additionally, transgenic tomato plants with VvPL15 showed accelerated fruit ripening and softening. Thus, VvPL15 plays a crucial role in grape berry softening by depolymerizing pectin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Mercy W. Mwaniki, Oscar W. Mitalo, Eric G. Mworia, Willis O. Owino, Kyoko Hiwasa-Tanase, Jocelyn K. C. Rose, Koh Aoki, Tomoya Esumi, Takashi Kawai, Ryohei Nakano, Koichiro Ushijima, Yasutaka Kubo
Summary: The study identified potential genes encoding secreted proteins that control pear fruit softening using a yeast-based signal sequence trap and macroarray gene expression analysis. Differential softening behaviors of European and Chinese pear fruits during ripening may be associated with specific ethylene-regulated CDNA encoding cell wall modifying proteins.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Zhu Zi-chao, Luo Shang, Lei Bin, Li Xian-yong, Cheng Zhi-jun
Summary: Rice panicle apical abortion (PAA) is a detrimental trait that reduces spikelet number and yield in rice. The LOC_Os10g31910 gene has been identified as the causative locus TUTOU2 responsible for the PAA phenotype, and it may also be the locus for the previously documented leaf senescence gene DEL1.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Biotechnology & Applied Microbiology
Leandro Lopes da Silva, Tulio Morgan, Ediones Amaro Garcia, Rafael Oliveira Rosa, Tiago Antonio de Oliveira Mendes, Marisa Vieira de Queiroz
Summary: This study aimed to identify and analyze genes encoding pectinases in the genome of the fungus Colletotrichum lindemuthianum, evaluate their expression, and compare them with pectinases from other fungi. The results showed that C. lindemuthianum has a rich variety of pectinase genes, which may play a crucial role in its pathogenicity. The findings contribute to a better understanding of the importance of pectinases in common bean infection and have potential applications in biotechnology.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ling Zheng, Zilong Guo, Yinxiao Xu, Benwei Zhu, Zhong Yao
Summary: The pectate lyase (ErPL2) from Echinicola rosea JL3085 exhibited optimal activity at 45°C and pH 9.0. Immobilization of ErPL2 onto mesoporous titanium oxide particles (MTOPs) significantly improved its thermal stability, pH operation range, and catalytic efficiency.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Josip Safran, Vanessa Ung, Julie Bouckaert, Olivier Habrylo, Roland Molinie, Jean-Xavier Fontaine, Adrien Lemaire, Aline Voxeur, Serge Pilard, Corinne Pau-Roblot, Davide Mercadante, Jerome Pelloux, Fabien Senechal
Summary: This study characterized VdPelB, an enzyme from Verticillium dahliae, and found that it exhibited high activity on non-methylesterified pectins, with activity influenced by pH and Ca2+ ions. The released oligogalacturonides (OGs) by VdPelB differed from other characterized Pectate lyases, indicating its unique specificity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Qian Deng, Shi-Qi Li, Xiao-Bao Sun, De-Ying Gao, Nuo Li, Hui-En Zhang, Zheng-Guang Wang, Jia-Kun Wang, Qian Wang
Summary: This study cloned two pectate lyase genes, IDSPel16 and IDSPel17, from a sheep rumen microbiome and characterized their functional properties. The results showed that IDSPel16 had relatively higher stability and activity, making it potentially useful in the textile, food, and feed industries.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yun Bai, Dongdong Tian, Peng Chen, Dan Wu, Kebing Du, Bo Zheng, Xueping Shi
Summary: Pectin structure changes can have serious consequences on plant growth and development by affecting the formation of the secondary cell wall. In this study, four pectate lyase-like protein genes were identified as being important for vascular development in Arabidopsis. Knock-out of one of these genes, AtPLL16, resulted in an irregular xylem phenotype and increased pectin, soluble sugar, and acid-soluble lignin levels. Overexpression of AtPLL16 led to expansion of the phloem and a dramatic change in the xylem-to-phloem ratios.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Chenggang Liang, Chunyu Wei, Li Wang, Zhixiu Guan, Taoxiong Shi, Juan Huang, Bin Li, Yang Lu, Hui Liu, Yan Wang
Summary: Gravity is an important environmental factor involved in the regulation of plant architecture. A study on the creeping growth of Tartary buckwheat mutant lazy1 revealed that the mutation affected shoot negative gravitropism and resulted in dilated and distorted cells in the bending stem. The expression of gibberellin-regulated genes was up-regulated in lazy1, suggesting the involvement of gibberellin in the regulation of creeping growth. The deletion of the coding region of Pectate lyase 5 in lazy1 disrupted the pbH domain and led to abnormal cell elongation. The study highlights the role of Pectate lyase 5 in mediating primary cell wall metabolism and regulating asymmetric cell elongation in lazy1.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Ying Yang, Laifeng Lu, Dandan Sun, Jinghao Wang, Nifei Wang, Liping Qiao, Qingbin Guo, Changlu Wang
Summary: The study showed that OGs generated by fungal PG could delay tomato fruit softening and maintain fruit firmness; RNA sequencing data confirmed the involvement of pathways like ethylene and jasmonic acid signals; Suppressing the expression of ethylene response factor SlERF6 could attenuate the softening process restrained by OG.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Max Bush, Vishmita Sethi, Robert Sablowski
Summary: The plant vasculature plays crucial roles in water and nutrient transport. Pectins, key components of cell walls, are modified during cell growth and differentiation. This study reveals the importance of the pectin lyase-like gene PLL12 in the growth of vascular bundles in Arabidopsis.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Environmental Sciences
Guanglong Chen, Qingyuan Feng, Jun Wang
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Biochemistry & Molecular Biology
Yu Zou, Guanglong Chen, Jing Jin, Ying Wang, Meiling Xu, Jing Peng, Yi Ding
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Chemistry, Analytical
Guanglong Chen, Zhilu Fu, Huirong Yang, Jun Wang
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Environmental Sciences
Yizheng Li, Guanglong Chen, Kaihang Xu, Kai Huang, Jun Wang
Summary: This study investigated microplastics in aquaculture ponds in the Pearl River Estuary, revealing their presence in water and sediment samples, as well as in the gastrointestinal tracts of fishes and shrimps. The main components identified were cellulose, polypropylene (PP), and polyethylene (PE), with microplastics of <1 mm in size and fiber shape being the most common. Results of potential risk assessment suggested that evaluating microplastic pollution status based on toxicity score and abundance may lead to more reliable results.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Green & Sustainable Science & Technology
Yizheng Li, Yindan Zhang, Guanglong Chen, Kaihang Xu, Han Gong, Kai Huang, Muting Yan, Jun Wang
Summary: Microplastics pollution levels in coastal areas of Guangdong Province are high, with transparent fibers being the main type. The study evaluated the pollution risk of microplastics along the coast of Guangdong using Raman analysis and pollution load index method, providing a basis for the formulation of environmental policy.
Review
Environmental Sciences
Huirong Yang, Guanglong Chen, Jun Wang
Summary: The severe global microplastic pollution, especially in the marine environment, has raised public concern. Microplastics have negative impacts on marine organisms and eventually human health, requiring measures to address environmental problems caused by them.
Review
Environmental Sciences
Guanglong Chen, Yizheng Li, Jun Wang
Summary: The extensive use of plastics in human life has led to significant microplastic pollution globally, particularly in aquaculture, which has raised concerns for its ecological impacts on aquaculture species and potential health risks for humans. Aquatic products have become a primary source of human exposure to microplastics due to the contamination in aquaculture environments.
Review
Environmental Sciences
Guanglong Chen, Yizheng Li, Shulin Liu, Muhammad Junaid, Jun Wang
Summary: Microplastic/nanoplastic pollution has serious impacts on higher plants and surrounding ecosystems, causing oxidative stress, affecting photosynthesis, metabolism, and altering soil properties and microbial communities. Additional studies are needed to further understand the full extent of the effects on the ecosystem and human health.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Agronomy
Jihong Hu, Liyu Huang, Guanglong Chen, Hui Liu, Yesheng Zhang, Ru Zhang, Shilai Zhang, Jintao Liu, Qingyi Hu, Fengyi Hu, Wen Wang, Yi Ding
Summary: OsSPL4 is a key regulator in panicle and grain development of rice, controlling grain size and number by promoting cell division and potentially influencing rice yield. It can also regulate multiple genes related to cell cycle and MADS-box genes to affect grain size in rice.
Review
Fisheries
Zhen Li, Muhammad Junaid, Guanglong Chen, Jun Wang
Summary: Microplastics, antibiotics, and heavy metals in aquaculture environments have serious ecological and human health consequences. Microorganisms develop antibiotic resistance genes and metal resistance genes as a defense mechanism. Heavy metals may lead bacteria to employ antibiotic resistance, while microplastics could act as potential carriers of multidrug-resistant genes.
REVIEWS IN AQUACULTURE
(2022)
Review
Agriculture, Multidisciplinary
Jihong Hu, Guanglong Chen, Kui Xu, Jun Wang
Summary: This review summarizes the processes of cadmium uptake, translocation, and accumulation in cereal crops, as well as efficient measures to reduce cadmium uptake and potential remediation strategies. The findings provide insights into innovative approaches for reducing cadmium accumulation in cereal grains and sustainable management of cadmium-contaminated paddy fields.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Environmental Sciences
Huirong Yang, Han Dong, Yurou Huang, Guanglong Chen, Jun Wang
Summary: This review summarizes the research progress on the source, accumulation, degradation, and migration of microplastics (MPs) in soil, as well as their potential risks to the ecological environment and food chain. However, little attention has been paid to the environmental behavior, ecotoxicology, and interaction with biological and chemical pollutants in soil.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Environmental Sciences
Dandan Gao, Xinyu Liu, Muhammad Junaid, Hongping Liao, Guanglong Chen, Yan Wu, Jun Wang
Summary: This article focuses on the toxic effects of micro(nano)plastics (MNPs) on benthic organisms, summarizing the accumulation of MNPs in benthic organisms and the resulting tissue damage, genotoxicity, and reproductive toxicity. The complexity of co-exposure to MNPs with other aquatic contaminants is highlighted, and the internal and external factors affecting the toxicity of MNPs in benthic organisms are discussed. Overall, this study provides important background information for a thorough understanding of the toxic effects of MNPs in the benthic aquatic ecosystem.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Hongping Liao, Shulin Liu, Muhammad Junaid, Dandan Gao, Wenjie Ai, Guanglong Chen, Jun Wang
Summary: This study demonstrates that the presence of DEHP enhances the toxicity of nanoplastics on juvenile largemouth bass, resulting in decreased feeding and growth rates, structural damage to the intestine and liver, oxidative stress, and changes in intestinal microbial composition. The findings highlight the importance of regulating the use of plastic and its additives to improve the health status of aquaculture fish for food safety.
Article
Environmental Sciences
Zhilu Fu, Guanglong Chen, Wenjing Wang, Jun Wang
ENVIRONMENTAL POLLUTION
(2020)