Article
Immunology
Zsofia Agnes Bittner, Xiao Liu, Maria Mateo Tortola, Ana Tapia-Abellan, Sangeetha Shankar, Liudmila Andreeva, Matthew Mangan, Marianne Spalinger, Hubert Kalbacher, Peter Duewell, Marta Lovotti, Karlotta Bosch, Sabine Dickhoefer, Ana Marcu, Franziska Herster, Yamel Cardona Gloria, Tzu-Hsuan Chang, Francesca Bork, Carsten L. Greve, Markus W. Loeffler, Olaf-Oliver Wolz, Nadine A. Schilling, Jasmin B. Kuemmerle-Deschner, Samuel Wagner, Anita Delor, Bodo Grimbacher, Oliver Hantschel, Michael Scharl, Hao Wu, Eicke Latz, Alexander N. R. Weber
Summary: The study reveals that Bruton's tyrosine kinase (BTK) affects multiple levels of NLRP3 regulation by interacting with NLRP3 and phosphorylating conserved tyrosine residues upon inflammasome activation, promoting NLRP3 relocalization, oligomerization, ASC polymerization, and inflammasome assembly. This suggests BTK as a multifunctional positive regulator of NLRP3 regulation and its phosphorylation of NLRP3 as a potential therapeutic target for controlling inflammation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Fei He, Qi Yu, Min Wang, Rongsha Wang, Xuanyunjing Gong, Feng Ge, Xilan Yu, Shanshan Li
Summary: This study reveals the inhibitory effect of H3pT11 on H3K79me3 and uncovers the histone crosstalk regulating autophagy and telomere silencing. It is also discovered that Reb1 recruits the SESAME complex to telomere regions to prevent the invasion of H3K79me3 into heterochromatin, maintaining telomere silencing.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Anil Kumar Vijjamarri, Neha Gupta, Chisom Onu, Xiao Niu, Fan Zhang, Rakesh Kumar, Zhenguo Lin, Miriam L. Greenberg, Alan G. Hinnebusch
Summary: We investigated the roles of Pat1 and Dhh1 in regulating mRNA translation and abundance in yeast cells under nutrient-replete conditions. Our findings suggest that Pat1 and Dhh1 cooperatively repress the expression of numerous non-ESR transcripts by reducing decapping and transcription in wild-type cells. Furthermore, we observed impaired mRNA turnover in mutants lacking Dhh1 or Pat1, leading to derepression of these transcripts. Pat1 and Dhh1 also contribute to translational repression and decreased protein production for multiple pathways involved in cell adhesion, nitrogen metabolism, respiration, and autophagy. These findings highlight the post-transcriptional regulatory functions of Pat1 and Dhh1 during nutrient limitation.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Wentao Wang, Yue Lu, Junjie Li, Xinran Zhang, Fangfang Hu, Yu Zhao, Dao-Xiu Zhou
Summary: This study demonstrates that rice JMJ705 is a key factor in low energy stress tolerance, interacting with and regulating transcriptional networks in conjunction with SnRK1. JMJ705 directly targets a set of low energy stress-responsive transcription factor genes through H3K27me3 demethylation activity.
Article
Multidisciplinary Sciences
Tian Zhou, Zhiying Liang, Mario Andrea Marchisio
Summary: In this study, the working of a ss-estradiol-sensing device was optimized by engineering both the chimeric activator and the reporter protein expression cassette. The researchers found a configuration that functions as a highly sensitive biosensor and a sharp switch depending on the concentration of the chimeric activator and the strength of its activation domain.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Antonio Marsella, Elisa Gobbini, Corinne Cassani, Renata Tisi, Elda Cannavo, Giordano Reginato, Petr Cejka, Maria Pia Longhese
Summary: The MRX complex regulates its DNA binding and processing activities through transitions between different states, with Sae2 stimulating Mre11 endonuclease activity while Rif2 inhibits it. This dynamic regulation affects the retention of MRX at DSBs and checkpoint activation.
Article
Cell Biology
Xinyu Zhang, Qi Yu, Yinsheng Wu, Yuan Zhang, Yi He, Rongsha Wang, Xilan Yu, Shanshan Li
Summary: The mechanism by which cells adjust gene expression in response to nutritional changes is not well understood. This study identifies Glc7, a protein phosphatase 1 (PP1), as the enzyme that specifically dephosphorylates H3T11, and characterizes two Glc7-containing complexes involved in regulating gene expression under glucose starvation. These complexes, Glc7-Sen1 and Glc7-Rif1-Rap1, dephosphorylate H3T11 to activate the transcription of autophagy-related genes and derepress telomere-proximal genes, respectively. Furthermore, Glc7 expression is up-regulated and translocation into the nucleus is increased upon glucose starvation, leading to induction of autophagy and derepressed transcription of telomere-proximal genes. The functions of PP1/Glc7 and the two Glc7-containing complexes are also conserved in mammals, regulating autophagy and telomere structure.
Article
Optics
Yunqing Guan, Xiaopeng Dong
Summary: The optical Vernier effect is a powerful tool for increasing the sensitivity of an interferometer. However, a large measurement range can lead to a decrease in sensitivity due to the increased optical path difference. To overcome this, a novel method is proposed, which uses a virtual reference cell with a variable cavity length to adapt to the measurement range. Theoretical analysis and experimental measurements show that by adjusting the cavity length of the reference interferometer, the Vernier effect based sensitivity can be maintained at a high level.
OPTICS COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Siyeon Rhee, Jae I. Chung, Devin A. King, Gaetano D'amato, David T. Paik, Anna Duan, Andrew Chang, Danielle Nagelberg, Bikram Sharma, Youngtae Jeong, Maximilian Diehn, Joseph C. Wu, Ashby J. Morrison, Kristy Red-Horse
NATURE COMMUNICATIONS
(2018)
Article
Cell Biology
Graeme J. Gowans, Alicia N. Schep, Ka Man Wong, Devin A. King, William J. Greenleaf, Ashby J. Morrison
Article
Genetics & Heredity
Sean L. Beckwith, Erin K. Schwartz, Pablo E. Garcia-Nieto, Devin A. King, Graeme J. Gowans, Ka Man Wong, Tessa L. Eckley, Alexander P. Paraschuk, Egan L. Peltan, Laura R. Lee, Wei Yao, Ashby J. Morrison
Article
Biochemistry & Molecular Biology
Graeme J. Gowans, Joseph B. Bridgers, Jibo Zhang, Raghuvar Dronamraju, Anthony Burnetti, Devin A. King, Aline Thiengmany, Stephen A. Shinsky, Natarajan Bhanu, Benjamin A. Garcia, Nicolas E. Buchler, Brian D. Strah, Ashby J. Morrison
Review
Endocrinology & Metabolism
Ashby J. Morrison
MOLECULAR METABOLISM
(2020)
Article
Genetics & Heredity
Kadir C. Akdemir, Victoria T. Le, Justin M. Kim, Sarah Killcoyne, Devin A. King, Ya-Ping Lin, Yanyan Tian, Akira Inoue, Samirkumar B. Amin, Frederick S. Robinson, Manjunath Nimmakayalu, Rafael E. Herrera, Erica J. Lynn, Kin Chan, Sahil Seth, Leszek J. Klimczak, Moritz Gerstung, Dmitry A. Gordenin, John O'Brien, Lei Li, Yonathan Lissanu Deribe, Roel G. Verhaak, Peter J. Campbell, Rebecca Fitzgerald, Ashby J. Morrison, Jesse R. Dixon, P. Andrew Futreal
Article
Cardiac & Cardiovascular Systems
Siyeon Rhee, David T. Paik, Johnson Y. Yang, Danielle Nagelberg, Ian Williams, Lei Tian, Robert Roth, Mark Chandy, Jiyeon Ban, Nadjet Belbachir, Seokho Kim, Hao Zhang, Ragini Phansalkar, Ka Man Wong, Devin A. King, Caroline Valdez, Virginia D. Winn, Ashby J. Morrison, Joseph C. Wu, Kristy Red-Horse
Summary: The study identified pathological endocardial cell population and dysregulated angiocrine factors in non-compaction cardiomyopathy, which significantly affected cardiomyocyte behavior. The deletion of chromatin remodeller gene Ino80 inhibited cardiomyocyte proliferation and promoted maturation, suggesting a model where coronary endothelial cells promote myocardial compaction through secreted factors.
EUROPEAN HEART JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Ashby J. Morrison
Summary: Cellular adaptation to nutrient environment is crucial for survival, failure to adapt can result in cell death and diseases. Metabolism alterations, particularly in cancer cells, play a significant role in the pathogenesis of diseases. Recent studies have shown the interplay between metabolic pathways, epigenetic modifications, and transcriptional regulation in cancer cells.
Article
Biotechnology & Applied Microbiology
Brian S. Perez, Ka Man Wong, Erin K. Schwartz, Rafael E. Herrera, Devin A. King, Pablo E. Garcia-Nieto, Ashby J. Morrison
Summary: This study found a high degree of similarity between UV-induced 6-4PP and CPD formation, both enriched in heterochromatin regions. However, bivalent and Polycomb-repressed chromatin states were uniquely more susceptible to 6-4PPs. Disparate patterns were observed when comparing UV susceptibility, repair, and melanoma mutation frequency, indicating complex associations in these regions. Functional enrichment analysis hinted at negative selection mechanisms in regions essential for cell viability, immune function, and inducing cell death when mutated.
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
(2021)
Article
Multidisciplinary Sciences
Matthew A. Cottee, Sean L. Beckwith, Suzanne C. Letham, Sarah J. Kim, George R. Young, Jonathan P. Stoye, David J. Garfinkel, Ian A. Taylor
Summary: Excessive replication of Saccharomyces cerevisiae Ty1 retrotransposons is regulated by Copy Number Control, with p22/p18 protein interrupting Gag function to block retrotransposition. The authors present structural, biophysical and genetic analyses of p18m, a minimal fragment of Gag that restricts transposition, revealing insights into Ty1 Gag structure and the mechanism of action of p22/p18 in restriction.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Ka Man Wong, Devin A. King, Erin K. Schwartz, Rafael E. Herrera, Ashby J. Morrison
Summary: Carcinogen susceptibility, regulated by RB1 tumor suppressor, plays an important role in cancer mutagenesis. Deletion of RB1 increases UV lesion susceptibility in centromere and telomere-proximal regions, leading to accelerated mutagenic potential in cancers.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biotechnology & Applied Microbiology
Pablo E. Garcia-Nieto, Ashby J. Morrison, Hunter B. Fraser