Article
Immunology
Aihua Xu, Yang Yang, Yang Shao, Manyu Jiang, Yongxin Sun, Bo Feng
Summary: The study demonstrates that FHL2 plays a regulatory role in the inflammatory response following spinal cord injury by modulating microglia polarization, leading to improved functional recovery.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Ewa Brzozowska, Sameer Deshmukh
Summary: Integrins are crucial for cell adhesion, migration, and positioning. Studies have shown that the expression levels of Integrin αVβ6 are closely related to malignant diseases and patient prognosis. It plays a significant role in cancer progression and the activation of transforming growth factor β(TGF-β). While essential for normal function in healthy individuals, it can also be a target for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Jindao Wu, Wen Gao, Qiyun Tang, Yue Yu, Wei You, Zhengshan Wu, Ye Fan, Long Zhang, Chen Wu, Guoyong Han, Xueliang Zuo, Yao Zhang, Zhiqiang Chen, Wenzhou Ding, Xiangcheng Li, Fengming Lin, Hongbing Shen, Jinhai Tang, Yaqin Zhang, Xuehao Wang
Summary: This study showed that TAMs are enriched in HCC and promote the migratory potential of HCC cells through M2-derived exosomes. The specific and efficient presence of CD11b/CD18 in M2 exos was identified using mass spectrometry. Blocking CD11b and/or CD18 significantly decreased M2 exos-mediated HCC cell metastasis, highlighting the potential role of TAM-derived exosomes in promoting tumor migration.
Article
Biochemistry & Molecular Biology
Nicole M. Naranjo, Israa Salem, Maisha A. Harris, Lucia R. Languino
Summary: The reduction of the avi36 integrin subunit increases the expression of IFIT3 in prostate cancer cells and their derived sEVs. Cells lacking the i36 integrin subunit show high expression of IFIT3 and STAT1, with IFIT3 present in sEVs but not STAT1. Loss of IFIT3 leads to enriched STAT1 in sEVs as a compensatory mechanism.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Chunlu Li, Chengsi Deng, Tingting Zhou, Jiapeng Hu, Bing Dai, Fei Yi, Na Tian, Lijun Jiang, Xiang Dong, Qingfeng Zhu, Siyi Zhang, Hongyan Cui, Liu Cao, Yunxiao Shang
Summary: The study revealed that M2 macrophagederived exosomes can alleviate asthma progression by downregulating FGF1 expression and the MAPK/STAT1 signaling pathway through carrying miR-370. This mechanism provides a new perspective for asthma treatment.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Microbiology
Mireya Becerra-Diaz, Yadira Ledesma-Soto, Jonadab E. Olguin, Angel Sanchez-Barrera, Monica G. Mendoza-Rodriguez, Sandy Reyes, Abhay R. Satoskar, Luis I. Terrazas
Summary: In this study, it was found that STAT1(-/-) mice exhibited increased resistance to cestode infection, likely due to impaired recruitment of CD11b(+)CCR2(+)Ly6C(hi)Ly6G(-) cells and failure to develop M2 macrophages.
Article
Neurosciences
Cheng Li, Xiaoxiao Li, Zhen Shi, Panfeng Wu, Jinfei Fu, Juyu Tang, Liming Qing
Summary: Our study reveals that LPS preconditioned mesenchymal stem cell-derived exosomes (LPS pre-Exos) can promote functional recovery and repair in peripheral nerve injury by accelerating axon regeneration and remyelination, as well as enhancing macrophage polarization. TSG-6, highly enriched in LPS pre-Exos, serves as a critical mediator in inducing the regulatory effects by inhibiting NF-κB and NLRP3 signaling pathways.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Valerie Pireaux, Cedric Delporte, Alexandre Rousseau, Jean-Marc Desmet, Pierre Van Antwerpen, Martine Raes, Karim Zouaoui Boudjeltia
Summary: Hemodialyzed patients exhibit significantly higher concentrations of inflammatory biomarkers such as CRP, M-CSF, and IL-8, as well as myeloperoxidase-associated oxidative stress biomarkers like chloro-tyrosine and homocitrulline. In addition, there is a greater percentage of M2 monocytes in the plasma of hemodialysis patients compared to controls.
Article
Cell Biology
Ai-Hua Xu, Yang Yang, Yang Shao, Man-Yu Jiang, Yong-Xin Sun
Summary: The role of PARP14 in spinal cord injury (SCI) was investigated, and it was found that PARP14 up-regulation promoted functional recovery after SCI by regulating the phenotypic transformation of microglia via the STAT1/6 pathway.
NEURAL REGENERATION RESEARCH
(2023)
Review
Immunology
Lisa Hirahara, Kaoru Takase-Minegishi, Yohei Kirino, Yuki Iizuka-Iribe, Yutaro Soejima, Ryusuke Yoshimi, Hideaki Nakajima
Summary: Behcet's disease is a systemic inflammatory disease characterized by recurrent oral ulcers, genital ulcers, cutaneous inflammation, and uveitis. The pathogenesis of BD is not fully understood, but it is likely a result of genetic susceptibility and environmental factors. This review discusses the roles of monocytes and macrophages in BD and their potential as therapeutic targets.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Medicinal
Yi Zhang, Xiujin Shi, Jialun Han, Wenxing Peng, Zhenwei Fang, Yang Zhou, Xiaoyu Xu, Jie Lin, Fucheng Xiao, Limin Zhao, Yang Lin
Summary: This study found that CNT can reduce atherosclerotic lesions, promote M2 macrophage polarization, and inhibit inflammatory responses. CNT exerts its anti-atherosclerotic effect by activating the PPAR gamma, Integrin alpha(v), and Integrin beta(5) signaling pathways.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2021)
Article
Chemistry, Multidisciplinary
Hui Li, Yue Feng, Xiu Zheng, Ming Jia, Zhiqiang Mei, Yao Wang, Zhuo Zhang, Meiling Zhou, Chunhong Li
Summary: The combination therapy using M2 macrophage exosomes loaded with IL-10 pDNA and BSP was effective in targeting and reducing inflammation in RA. The synergistic effects of IL-10 pDNA and BSP promoted M1-to-M2 macrophage polarization, demonstrating high anti-inflammatory activity and therapeutic effect against RA.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Neurosciences
Chin-Hung Hsu, Yi-Ju Pan, Yin-Ting Zheng, Raymond Y. Lo, Feng-Yi Yang
Summary: This study investigated the effects of low-intensity pulsed ultrasound (LIPUS) on M1/M2 polarization of microglial cells and the regulatory mechanisms associated with signaling pathways. The results showed that LIPUS treatment attenuated inflammatory markers and enhanced M2-related markers, switching microglial cells from the M1 to the M2 phenotype.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Oncology
Zhiwei He, Jie Wang, Changhao Zhu, Jian Xu, Peng Chen, Xueyi Jiang, Yankun Chen, Jianxin Jiang, Chengyi Sun
Summary: In this study, it was found that PC cells generate exosomes rich in FGD5-AS1, which promote M2 macrophage polarization and malignant behaviors of PC cells. FGD5-AS1 activates the STAT3/ NF-Kappa B pathway and interacts with p300, leading to STAT3 acetylation and nuclear localization. These findings highlight the important role of exosomal FGD5-AS1 in PC.
Article
Biotechnology & Applied Microbiology
Wei Peng, Yong Xie, Zixiang Luo, Yudong Liu, Jiaqi Xu, Chengjun Li, Tian Qin, Hongbin Lu, Jianzhong Hu
Summary: Macrophages polarized to the M2 subtype after spinal cord injury (SCI) are beneficial for promoting neurological recovery. The crosstalk between endothelial cells (ECs) and macrophages is crucial for the imbalance between proinflammatory and pro-resolving responses caused by macrophage heterogeneity; however, this crosstalk is strengthened post-SCI, leading to inflammatory cascades and second damage.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Gastroenterology & Hepatology
Koelina Ganguly, Rakesh Bhatia, Sanchita Rauth, Andrew Kisling, Pranita Atri, Christopher Thompson, Raghupathy Vengoji, Shiv Ram Krishn, Dhananjay Shinde, Vinai Thomas, Sukhwinder Kaur, Kavita Mallya, Jesse L. Cox, Sushil Kumar, Surinder K. Batra
Summary: The study reveals that MUC5AC promotes gemcitabine resistance in pancreatic cancer cells by affecting glutamine metabolism and nucleotide biosynthesis. Depletion of MUC5AC and deprivation of glutamine can enhance sensitivity of pancreatic cancer cells to gemcitabine. Dual-targeting therapy against MUC5AC, beta-catenin, and glutamine metabolism can overcome gemcitabine resistance in pancreatic cancer.
Article
Oncology
Shiv Ram Krishn, Vaughn Garcia, Nicole M. Naranjo, Fabio Quaglia, Christopher D. Shields, Maisha A. Harris, Andrew Kossenkov, Qin Liu, Eva Corey, Dario C. Altieri, Lucia R. Languino
Summary: The alpha V beta 6 integrin is upregulated during prostate cancer progression and specifically expressed in androgen receptor-negative tumors. By delivering ITGB6-targeting siRNAs into PrCa cells using sEVs, the expression of beta 6 subunit can be downregulated, leading to reduced cell adhesion and migration in PrCa cells.
CANCER BIOLOGY & THERAPY
(2022)
Article
Genetics & Heredity
Amir Hossein Saeidian, Leila Youssefian, Jianhe Huang, Andrew Touati, Hassan Vahidnezhad, Luke Kowal, Matthew Caffet, Tamara Wurst, Jagmohan Singh, Adam E. Snook, Ellen Ryu, Paolo Fortina, Sharon F. Terry, Jonathan G. Schoenecker, Jouni Uitto, Qiaoli Li
Summary: This study reports the genetic findings from an international cohort of patients with heritable ectopic mineralization. Multiple gene variants were identified, including 159 distinct variants in the ABCC6 gene. The functional assessment of these variants highlighted the importance of determining pathogenicity through appropriate experimental systems.
GENETICS IN MEDICINE
(2022)
Article
Immunology
Jennifer L. Hope, Manzhi Zhao, Christopher J. Stairiker, Caoimhe H. Kiernan, Alison J. Carey, Yvonne M. Mueller, Marjan van Meurs, Inge Brouwers-Haspels, Dennis C. Otero, Eun-Ah Bae, Hannah A. Faso, Alex Maas, Hans de Looper, Paolo M. Fortina, Isidore Rigoutsos, Linda M. Bradley, Stefan J. Erkeland, Peter D. Katsikis
Summary: This study characterized the expression profile of miRNAs in antiviral CD8(+) T cells and found that miR-139 was highly downregulated in effector CTLs. However, the deficiency or overexpression of miR-139 did not affect the development and response of immune cells.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jagadish C. Ghosh, Michela Perego, Ekta Agarwal, Irene Bertolini, Yuan Wang, Aaron R. Goldman, Hsin-Yao Tang, Andrew Kossenkov, Catherine J. Libby, Lucia R. Languino, Edward F. Plow, Annamaria Morotti, Luisa Ottobrini, Marco Locatelli, David W. Speicher, M. Cecilia Caino, Joel Cassel, Joseph M. Salvino, Marie E. Robert, Valentina Vaira, Dario C. Altieri
Summary: The study reveals that many human tumors have reduced levels of Mic60, an essential scaffold of mitochondrial structure, which leads to the disruption of mitochondrial integrity. Surprisingly, these tumors show decreased cell proliferation, resistance to cell death, and activation of a gene expression program related to innate immunity and cytokine/chemokine signaling. This process induces epithelial-mesenchymal transition, tumor cell movement, and promotes metastatic dissemination.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Gastroenterology & Hepatology
Jeffrey A. Rappaport, Ariana A. Entezari, Adi Caspi, Signe Caksa, Aakash V. Jhaveri, Timothy J. Stanek, Adam Ertel, Joan Kupper, Paolo M. Fortina, Steven B. McMahon, James B. Jaynes, Adam E. Snook, Scott A. Waldman
Summary: The study revealed the corepression of GUCY2C ligand transcription by beta-catenin/TCF signaling and provided genomic strategies to reestablish hormone expression. This mechanism reflects a novel pathophysiological step in tumorigenesis.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Medicine, Research & Experimental
Amir Hossein Saeidian, Leila Youssefian, Charles Y. Huang, Fahimeh Palizban, Mahtab Naji, Zahra Saffarian, Hamidreza Mahmoudi, Azadeh Goodarzi, Soheila Sotoudeh, Fatemeh Vahidnezhad, Maliheh Amani, Narjes Tavakoli, Ali Ajami, Samaneh Mozafarpoor, Mehrdad Teimoorian, Saeed Dorgaleleh, Sima Shokri, Mohammad Shenagari, Nima Abedi, Sirous Zeinali, Paolo Fortina, Vivien Beziat, Emmanuelle Jouanguy, Jean-Laurent Casanova, Jouni Uitto, Hassan Vahidnezhad
Summary: This study presents a whole-transcriptome sequencing-based method that can simultaneously identify the cutaneous virome and the underlying inborn errors of immunity (IEI) genes by RNA-Seq on a single skin biopsy. The method allows for rapid and accurate diagnosis of patients with viral skin infections and potential genetic defects, providing guidance for further treatment.
Article
Medicine, Research & Experimental
Joseph Curry, Angela Alnemri, Ramez Philips, Michele Fiorella, Sarah Sussman, Robert Stapp, Charalambos Solomides, Larry Harshyne, Andrew South, Adam Luginbuhl, Madalina Tuluc, Ubaldo Martinez-Outschoorn, Athanassios Argiris, Alban Linnenbach, Jennifer Johnson
Summary: This study analyzed the cellular density and intercellular distances of CD8+ and FoxP3+ T-cells in head and neck squamous cell carcinoma samples. The results showed that the distribution of CD8+ and FoxP3+ cells was associated with treatment outcomes and pathological treatment response. Increased CD8+-FoxP3+ intercellular distances were closely related to pathological treatment response.
Review
Oncology
Michael Xu, Latese Evans, Candice L. Bizzaro, Fabio Quaglia, Cecilia E. Verrillo, Li Li, Julia Stieglmaier, Matthew J. Schiewer, Lucia R. Languino, William K. Kelly
Summary: Despite therapeutic advances in prostate cancer treatment, metastatic castration-resistant prostate cancer remains a challenge. Highly expressed proteins STEAP1-4 have been identified as significant drivers of prostate cancer aggressiveness and metastasis. However, their other biological functions and understanding of heterotrimers and homotrimers are limited. This review highlights the importance of further research on the role of STEAP1-4 in prostate cancer and their potential clinical applications.
Article
Multidisciplinary Sciences
Fabio Quaglia, Shiv Ram Krishn, Khalid Sossey-Alaoui, Priyanka Shailendra Rana, Elzbieta Pluskota, Pyung Hun Park, Christopher D. Shields, Stephen Lin, Peter McCue, Andrew Kossenkov, Yanqing Wang, David W. Goodrich, Sheng-Yu Ku, Himisha Beltran, William K. Kelly, Eva Corey, Maja Klose, Christine Bandtlow, Qin Liu, Dario C. Altieri, Edward F. Plow, Lucia R. Languino
Summary: This study reveals a novel pathway activated by αVβ3 that promotes NEPrCa differentiation. NgR2 associates with αVβ3 and promotes NED and anchorage-independent growth in PrCa cells. These findings provide important insights into the mechanistic understanding of PrCa progression towards a NE phenotype.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Srinivas Reddy Boreddy, Reshmi Nair, Prashant Kumar Pandey, Anshu Kuriakose, Shivakumar Bhadravathi Marigowda, Chaitali Dey, Arindam Banerjee, Hanumant Kulkarni, Milind Sagar, Shiv Ram Krishn, Shruthi Rao, A. R. Madhukara, Vinita Tiwari, Bhavna Alke, Prashantha Kumar Mv, Meena Shri, Chaitrali Dhamne, Sonal Patel, Pinky Sharma, Sankar Periyasamy, Jaya Bhatnagar, Moni Abraham, Ram Bhupal Reddy, Amritha Suresh, Suma Sreenivas, Nagaraja Govindappa, Praveen Reddy Moole, Usha Bughani, Seng-Lai Tan, Pradip Nair
Summary: The EGFR and TGFI3 signaling pathways play important roles in tumorigenesis, and their cross-talk contributes to cancer progression and drug resistance. Targeting both EGFR and TGFI3 concurrently could improve patient outcomes in various types of cancer.
Article
Biochemistry & Molecular Biology
Minjeong Yeon, Irene Bertolini, Ekta Agarwal, Jagadish C. Ghosh, Hsin-Yao Tang, David W. Speicher, Frederick Keeney, Khalid Sossey-Alaoui, Elzbieta Pluskota, Katarzyna Bialkowska, Edward F. Plow, Lucia R. Languino, Emmanuel Skordalakes, M. Cecilia Caino, Dario C. Altieri
Summary: In this study, it is found that the altered ubiquitination ligase Parkin in Parkinson's disease forms a complex with the regulator of cell motility Kindlin-2 at mitochondria of tumor cells. Parkin ubiquitinates Kindlin-2 leading to its proteasomal degradation, which inhibits tumor cell interactions with the extracellular matrix, migration, and invasion. However, Parkin does not affect tumor cell proliferation, cell cycle transitions, or apoptosis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Brittany MacTaggart, Marie Shimogawa, Marshall Lougee, Hsin-Yao Tang, E. J. Petersson, Anna Kashina
Summary: This study reports the first global screen for side-chain arginylation and generates and validates panarginylation antibodies for detecting side-chain arginylation in any amino acid sequence context. The study identifies a limited set of proteins that likely undergo ATE1-dependent side-chain arginylation and are enriched in specific cellular roles.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Brianna L. L. Hill, Alyssa N. N. Calder, Joseph P. P. Flemming, Yiyang Guo, Sydney L. L. Gilmore, Melissa A. A. Trofa, Sean K. K. Daniels, Torbjoern N. N. Nielsen, Laura K. K. Gleason, Zoya Antysheva, Ksenia Demina, Nikita Kotlov, Christopher J. H. Davitt, David M. M. Cognetti, George C. C. Prendergast, Adam E. E. Snook, Jennifer M. M. Johnson, Gaurav Kumar, Alban J. J. Linnenbach, Ubaldo Martinez-Outschoorn, Andrew P. P. South, Joseph M. M. Curry, Larry A. A. Harshyne, Adam J. J. Luginbuhl, My G. Mahoney
Summary: Therapy using anti-PD-1 immune checkpoint inhibitors has revolutionized cancer treatment, but only a fraction of patients respond. The study identified Dsg2, miR-146a, and IL-8 as potential biomarkers for ICI response in HPVpos HNSCC patients, suggesting that targeting the Dsg2/miR-146a/IL-8 signaling axis could improve ICI responsiveness.
MOLECULAR CARCINOGENESIS
(2023)
Article
Oncology
Simona Lombardi, Aaron R. Goldman, Hsin-Yao Tang, Andrew Kossenkov, Heng Liu, Wei Zhou, Meenhard Herlyn, Jianhuang Lin, Rugang Zhang
Summary: The arginine methyltransferase CARM1 is highly expressed in various human cancers, including ovarian cancer, but therapeutic approaches for tumors overexpressing CARM1 have not been studied. This study found that CARM1 promotes monounsaturated fatty acid synthesis and targeting the fatty acid reprogramming pathway can be a potential therapeutic strategy for CARM1-expressing ovarian cancers.
CANCER RESEARCH COMMUNICATIONS
(2023)