Article
Chemistry, Applied
Song Yi Koo, Keum Taek Hwang, Soonjae Hwang, Ki Young Choi, Yun Ji Park, Jae-Hyeong Choi, To Quyen Truong, Sang Min Kim
Summary: Nanoencapsulation significantly improves the bioavailability of fucoxanthin in microalgae. Both types of nanoparticles show controlled release of fucoxanthin and enhance its permeability and absorption in cells and plasma.
Article
Agricultural Engineering
Zhi-Peng Wang, Pei-Kang Wang, Yan Ma, Jia-Xue Lin, Cheng-Long Wang, Yu-Xiang Zhao, Xin-Yue Zhang, Bei-Chen Huang, Shou-Geng Zhao, Lei Gao, Jing Jiang, Hai-Ying Wang, Wei Chen
Summary: The study demonstrated that the addition of Laminaria japonica hydrolysate (LPH) is a feasible and efficient strategy to promote fucoxanthin accumulation. In the LPH group, both fucoxanthin content and biomass were significantly increased.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ting-Bin Hao, Yang Lu, Zhong-Hong Zhang, Si-Fen Liu, Xiang Wang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: This study successfully improved carotenoid production in diatom Phaeodactylum tricornutum through genetic modification, particularly by overexpressing key carotenogenic genes involved in the MEP pathway. The elevated expression of carotenogenic genes led to increased total carotenoid content, with significant enhancement in fucoxanthin content in the engineered lines CMK and CMS.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Yong Fan, Xiao-Ting Ding, Li-Juan Wang, Er-Ying Jiang, Phung Nghi Van, Fu-Li Li
Summary: This study introduces a new method for screening mutants with high fucoxanthin content, successfully identifying a linear correlation between content and fluorescence intensity in the diatom model organism rich in fucoxanthin. By using flow cytometry, high-fucoxanthin-content mutants were sorted out, with a significant increase in fucoxanthin content compared to the wild type after 20 days of cultivation. This efficient, rapid, and high-throughput approach provides a valuable tool for screening fucoxanthin-overproducing mutants.
Article
Biotechnology & Applied Microbiology
Hugo Pereira, Marta Sa, Ines Maia, Alexandre Rodrigues, Iago Teles, Rene H. Wijffels, Joao Navalho, Maria Barbosa
Summary: This study produced two marine microalgae to evaluate biomass and fucoxanthin production, demonstrating the potential for fucoxanthin production from microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Chaitanya Kumar Reddy Pocha, Wen Yi Chia, Kit Wayne Chew, Heli Siti Halimatul Munawaroh, Pau Loke Show
Summary: This paper discusses the extraction of fucoxanthin, focusing on Phaeodactylum tricornutum and briefly mentioning Chaetoceros calcitrans and Isochrysis galbana. The cultivation, harvesting, and drying processes of these diatoms are briefly discussed, followed by an introduction to traditional and new extraction methods. The limitations of current extraction strategies and the potential for cost-effective technologies are also discussed.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Xiaotong Shen, Kehou Pan, Lin Zhang, Baohua Zhu, Yun Li, Jichang Han
Summary: This study investigated the function of a protein called PtABA4 in the synthesis of fucoxanthin, a valuable carotenoid. The overexpression and knockdown of PtABA4 in Phaeodactylum tricornutum showed significant changes in fucoxanthin accumulation. It is speculated that PtABA4 may form multi-enzyme complexes with other membrane associated enzymes and participate in the formation of various carotenoids.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Xinjie Zhao, Liwei Gao, Xiangzhong Zhao
Summary: In this study, a two-step method was used to purify Fucoxanthin from Phaeodactylum tricornutum, resulting in a purity of 92.03% and a recovery rate of 76.67%. The identification and quantification of Fucoxanthin were determined by HPLC and ESI-MS. The purified Fucoxanthin exhibited strong antioxidant properties in vitro.
Article
Chemistry, Medicinal
Mari Carmen Ruiz-Dominguez, Francisca Salinas, Elena Medina, Barbara Rincon, Mari angeles Martin, Mari Carmen Gutierrez, Pedro Cerezal-Mezquita
Summary: In this study, fucoxanthin was successfully extracted from Phaeodactylum tricornutum using supercritical fluid extraction (SFE) and the extracted diatom-like substrate was utilized for bioenergy production through anaerobic digestion (AD) processes. The optimization of various factors resulted in high yield, purity, and recovery of fucoxanthin. The extracted diatom and the whole diatom both showed potential for biogas production, with different methane yields. Therefore, Phaeodactylum tricornutum can serve as a valuable source of both fucoxanthin and methane, contributing to the sustainability of algae-biorefinery processes.
Article
Multidisciplinary Sciences
A-Hyeon Lee, Hye-Yoon Shin, Jong-Hwi Park, Song Yi Koo, Sang Min Kim, Seung-Hoon Yang
Summary: Pro-inflammatory cytokines are produced and secreted through the activation of NF-kappa B and NLRP3 inflammasome signaling pathways. Fucoxanthin, found in Phaeodactylum tricornutum extracts, has been shown to inhibit the activation of these pathways and decrease the production of major pro-inflammatory cytokines.
SCIENTIFIC REPORTS
(2021)
Article
Agricultural Engineering
Qing He, Hanwen Zhang, Mingyang Ma, Yue He, Jing Jia, Qiang Hu, Yingchun Gong
Summary: This study identifies a heterolobosean amoeba as the most common and destructive predator in P. tricornutum cultures. The addition of 400 mg L-1 NH4HCO3 inhibits amoeba growth without significant impact on algal growth. Additionally, stopping culture mixing at night creates a hypoxia environment that further hinders amoeba growth. Consequently, the periodic supply of NH4HCO3 and the cessation of culture mixing at night can prevent or treat protozoan contamination in mass culture of P. tricornutum.
BIORESOURCE TECHNOLOGY
(2022)
Article
Plant Sciences
Jianquan Song, Hejing Zhao, Linxin Zhang, Zheng Li, Jichang Han, Chengxu Zhou, Jilin Xu, Xiaohui Li, Xiaojun Yan
Summary: This study revealed that the heat shock transcription factor PtHSF1 positively regulates the synthesis of triacylglycerol and fucoxanthin in Phaeodactylum tricornutum. Overexpression of PtHSF1 increased the contents of triacylglycerol and fucoxanthin and upregulated key enzyme genes involved in their biosynthesis pathways. Gene silencing of PtHSF1 had the opposite effect. This study provided insights into the regulation mechanisms of PtHSF1-mediated triacylglycerol and fucoxanthin synthesis.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Nutrition & Dietetics
Lena Stiefvatter, Ulrike Neumann, Andreas Rings, Konstantin Frick, Ulrike Schmid-Staiger, Stephan C. Bischoff
Summary: Microalgae such as Phaeodactylum tricornutum can be used as a sustainable and nutritious source, providing benefits such as increased body weight gain and improved composition of gut microbiota and short-chain fatty acids.
Article
Environmental Sciences
Runqing Yang, Dong Wei, Georg Pohnert
Summary: This study reveals that a combination of tryptone and urea as organic nitrogen sources can enhance fucoxanthin production in mixotrophic cultivation of Phaeodactylum tricornutum. Among the amino acids present in the medium, arginine has the highest nitrogen utilization rate and promotes cell growth the most. The optimal combination of arginine and urea as nitrogen sources leads to the highest biomass concentration, fucoxanthin content, and productivity. This study provides new insights into the promotion of fucoxanthin biosynthesis and suggests a promising strategy for efficient enhancement of fucoxanthin production.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Chemistry, Medicinal
Daniela Barcenas-Perez, Antonin Strizek, Pavel Hrouzek, Jiri Kopecky, Marta Barradas, Arantzazu Sierra-Ramirez, Pablo J. Fernandez-Marcos, Jose Cheel
Summary: This study developed a multiple sequential injection HPCCC method to isolate fucoxanthin from Phaeodactylum tricornutum with high purity and recovery. The isolated fucoxanthin retained its biological activity and may provide a useful model for producing fucoxanthin from diatom biomass. Environmental risk and general process evaluation factors were used to assess the developed separation method and compare it with existing methods.
Article
Chemistry, Physical
Huawei Geng, Zonggang Mou, Ziyong Liu, Fuli Li, Cheng Yang
Article
Biotechnology & Applied Microbiology
Hamed Hamouda, Nasir Ali, Hang Su, Jie Feng, Ming Lu, Fu-Li Li
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Environmental Sciences
Nasir Ali, Hamed Hamouda, Hang Su, Fu-Li Li, Ming Lu
ENVIRONMENTAL RESEARCH
(2020)
Article
Ecology
Huanhuan Wang, Xu Li, Xinyu Li, Fuli Li, Zhencheng Su, Huiwen Zhang
Summary: The research found that diazotrophs in paddy soils mainly belong to the phylum Proteobacteria, with the highest diversity in the 10-20 cm layer and decreasing with soil depth. The community structure of diazotrophs is significantly influenced by region and soil depth, with soil total C and N content and pH being the main factors affecting vertical variation.
Article
Biochemistry & Molecular Biology
Bing Wang, Sheng Dong, Fu-Li Li, Xiao-Qing Ma
Summary: Recent studies have shown that human gut microbes have the ability to utilize alginate, which is an important carbon source for marine life. While there have been numerous studies on alginate lyases from marine/soil organisms, research on alginate lyases from gut microbes is still limited. The structure of a PL6 alginate lyase from the human gut microbe Bacteroides clarus was solved, revealing insights into its catalytic mechanism and substrate binding characteristics.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Yong Fan, Xiao-Ting Ding, Li-Juan Wang, Er-Ying Jiang, Phung Nghi Van, Fu-Li Li
Summary: This study introduces a new method for screening mutants with high fucoxanthin content, successfully identifying a linear correlation between content and fluorescence intensity in the diatom model organism rich in fucoxanthin. By using flow cytometry, high-fucoxanthin-content mutants were sorted out, with a significant increase in fucoxanthin content compared to the wild type after 20 days of cultivation. This efficient, rapid, and high-throughput approach provides a valuable tool for screening fucoxanthin-overproducing mutants.
Article
Biochemistry & Molecular Biology
Xiao-Ting Ding, Yong Fan, Er-Ying Jiang, Xiao-Yi Shi, Eike Krautter, Guang-Rong Hu, Fu-Li Li
Summary: This study successfully improved the growth rate and eicosapentaenoic acid content of N. oceanica cells by introducing the Vitreoscilla hemoglobin gene, while reducing ROS levels and photorespiration levels. These results indicate that Vitreoscilla hemoglobin can enhance microalgal growth under stress conditions by mitigating ROS damage and modulating photorespiration.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Editorial Material
Microbiology
Eun Yeol Lee, Fu-Li Li, Yu Wang, Pramod P. Wangikar, Michael Thomas Guarnieri, Guodong Luan
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jihong Yi, Haiyan Huang, Jiyu Liang, Rufei Wang, Ziyong Liu, Fuli Li, Shuning Wang
Summary: The anaerobe Clostridium ljungdahlii can ferment CO or H-2/CO2 to produce acetate, ethanol, and 2,3-butanediol via the Wood-Ljungdahl pathway. The key enzyme methylenetetrahydrofolate reductase (MTHFR) was characterized as a non-electron-bifurcating heterodimer using reduced ferredoxin as its electron donor. These findings provide insight into the energy metabolism of syngas fermentation by C. ljungdahlii and may aid in the development of metabolic engineering strains and efficient fermentation technologies.
MICROBIOLOGY SPECTRUM
(2021)
Article
Genetics & Heredity
Jixuan Hong, Ziyue Meng, Zixi Zhang, Hang Su, Yuxuan Fan, Ruilin Huang, Ruirui Ding, Ning Zhang, Fuli Li, Shi'an Wang
Summary: This study comprehensively analyzed the editing outcomes induced by CRISPR-Cas9 treatment in yeast and identified diverse DNA repair patterns, including deletions, translocations, and point mutations. The analysis revealed that the on-target point mutations were not random and showed strong sequence dependence. The findings expand our knowledge of CRISPR-Cas9 mediated DNA repair.
Article
Biotechnology & Applied Microbiology
Ruilin Huang, Ruirui Ding, Yu Liu, Fuli Li, Zhaohui Zhang, Shi'an Wang
Summary: This study obtained a yellow mutant of Xanthophyllomyces dendrorhous through mutagenesis and identified the GATA transcription factor XdWC2 as a key regulator of carotenoid biosynthesis. XdWC2 interacts with XdWC1 to form a complex that regulates the expression of key genes involved in the synthesis of astaxanthin. The findings provide insights into the global regulation of astaxanthin biosynthesis and offer guidance for enhancing astaxanthin production in yeast strains.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Xinxin Zhang, Zhiheng Wang, Zhipeng Chen, Yuxiao Zhu, Ziyong Liu, Fuli Li, Wei Zhou, Zichao Dong, Jingxin Fan, Licheng Liu
Summary: The unique molecular trapdoor mechanism of chabazite zeolites provides an ideal platform for CO2 integrative adsorption and conversion. A mesoporous In-SSZ-13((MP)) catalyst was synthesized, which achieved high efficiency and current density for electrochemical CO2 reduction reaction (CO2RR) with uniformly dispersed indium active sites. Experimental results combined with DFT calculations reveal the reaction mechanism of In-SSZ-13((MP)) electrocatalytic reduction of CO2.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2022)
Article
Chemistry, Applied
Xinxin Zhang, Yuxiao Zhu, Ziyong Liu, Fuli Li, Wei Zhou, Zichao Dong, Jingxin Fan, Licheng Liu, Chunhua Du
Summary: The combination of a powerful CO2-enriching carrier and a robust active component provides a new idea for the construction of efficient catalysts for electrocatalytic CO2 reduction. In this study, a Cu-In/PNGC composite catalyst is obtained by using perforated nitrogen-rich graphene-like carbon nanolayers (PNGC) as the carrier and In-doped Cu2(OH)3(NO3) nanosheet patches as the active component. The Cu-In/PNGC catalyst exhibits high Faradaic efficiency and remarkable CO partial current density for electrocatalytic CO2 reduction.
JOURNAL OF ENERGY CHEMISTRY
(2022)
Review
Food Science & Technology
Nghi Van Phung, Fei Rong, Wan Yue Xia, Yong Fan, Xian Yu Li, Shi An Wang, Fu Li Li
Summary: Nervonic acid, a 24-carbon fatty acid, is abundant in human brain, liver, and kidney. It functions as a critical component of sphingolipids and has various health benefits. Studies have shown that nervonic acid supplementation can improve neurological diseases, cancers, diabetes, obesity, and their complications. Understanding the mechanisms of nervonic acid is important for potential therapeutic approaches, although research in this area is limited.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Wen-Zhu Tang, Dan-Dan Jiang, Yi-Xuan Fan, Quan Zhang, Li-Cheng Liu, Fu-Li Li, Zi-Yong Liu
Summary: Clostridium cellulovorans, an anaerobic butyrate-producing bacterium, can secrete various extracellular enzymes to degrade plant-based cellulose. However, it still secretes a large amount of protein in the broth when glucose is used as the carbon source. The metabolism and regulation of protein secretion need further investigation.
FERMENTATION-BASEL
(2023)
