Journal
LANGMUIR
Volume 34, Issue 22, Pages 6546-6555Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.8b00976
Keywords
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Funding
- European Commission (Erasmus Mundus Action 2 India4 EU II Grant) [Indi1200061]
- LabEx Tec 21 (Investissements d'Avenir Grant) [ANR-11-LABX-0030]
- Region Rhone-Alpes (ERDF: European Regional Development Fund)
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Enzyme cocktails of reusable, highly stable cellulolytic enzymes play an inevitable role in bioconversion of biomass to biofuels economically Cellulase, xylanase and beta-l,3-glucanase bound silica-amine functionalized iron oxide magnetic nanoparticles (ISN-CLEAs) were prepared and used as the biocatalyst for the depolymerization of cellulosic biomass into monomeric sugar in the present study. The Fe3O4-NPs and Fe3O4@SiO2-NH2-NPs and ISN-CLEAs had an average hydrodynamic size of 82.2, 86.4, and 976.9 nm, respectively, which was confirmed by dynamic light scattering (DLS). About 97% of protein binding was achieved with 135 mM glutaraldehyde at 10 h of cross-linking time and successful binding was confirmed by Fourier transform infrared spectroscopy (FTIR). The ISN-CLEAs exhibited the highest thermal stability of 95% at 50 C-degrees for 2 h and retained extended storage stability of 97% compared to 60% of its free counterpart. Besides, cross-linking allowed ISN-CLEAs reuse for at least eight consecutive cycles retaining over 70% of its initial activity. ISN-CLEAs exhibited approximately 15% increase in carbohydrate digestibility on sugar cane bagasse and eucalyptus pulp than the free enzyme.
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