Article
Veterinary Sciences
Metka Voga, Valerija Kovac, Gregor Majdic
Summary: The study compared the characteristics of adipose-derived MSCs from dogs and domestic cats, revealing a higher percentage of CD44(+)CD90(+) and CD34(-/-) cells in canine MSCs. Additionally, canine ADMSCs demonstrated greater proliferation and differentiation potential compared to feline ADMSCs.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Cell & Tissue Engineering
Guanyin Chen, Dong Fan, Wangqian Zhang, Shuning Wang, Jintao Gu, Yuan Gao, Lei He, Weina Li, Cun Zhang, Meng Li, Yingqi Zhang, Zhaohui Liu, Qiang Hao
Summary: This study found that under hypoxic conditions, there was a significant increase in the expression of Mkx in MSCs. Mkx mediated hypoxia-induced tenogenic differentiation of MSCs but could not completely repress the proliferation of hypoxic MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jiajia Xu, Zhao Li, Robert J. Tower, Stefano Negri, Yiyun Wang, Carolyn A. Meyers, Takashi Sono, Qizhi Qin, Amy Lu, Xin Xing, Edward F. McCarthy, Thomas L. Clemens, Aaron W. James
Summary: Bone regeneration following injury is coordinated by inflammatory signals and sensory nerve infiltration. This study demonstrates that nerve growth factor (NGF) signals through p75 to affect migration of mesenchymal osteogenic precursors, thereby impacting bone healing. Lack of NGF or p75 results in delayed bone repair.
Article
Multidisciplinary Sciences
Simon Decker, Marcela Arango-Ospina, Felix Rehder, Arash Moghaddam, Rolf Simon, Christian Merle, Tobias Renkawitz, Aldo R. Boccaccini, Fabian Westhauser
Summary: This study investigates the influence of boron-doped bioactive glasses (BGs) on angiogenesis and osteogenesis. The results show that boron doping has a slight stimulatory effect on in vitro cellular osteogenic differentiation and angiogenesis, while significantly enhancing vascularization in vivo. The implementation of new experimental models, such as extending the use of the chorioallantoic membrane (CAM) assay, is important for studying the 'angiogenic-osteogenic coupling' mechanisms.
SCIENTIFIC REPORTS
(2022)
Article
Nanoscience & Nanotechnology
Zijun Deng, Weiwei Wang, Xun Xu, Yan Nie, Yue Liu, Oliver E. C. Gould, Nan Ma, Andreas Lendlein
Summary: The use of a PDA-coated substrate can reduce oxidative stress and cellular senescence in stem cell expansion, while promoting proliferation rate. PDA also helps suppress the expression of senescence-associated inflammatory components, while maintaining stem cell characteristics and differentiation potential.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Yadi Wang, Wentao Dai, Zhixiao Liu, Jixiang Liu, Jie Cheng, Yuanyuan Li, Xueling Li, Jun Hu, Junhong Lu
Summary: A method based on single-cell FTIR microspectroscopy was developed to quantitatively evaluate cellular heterogeneity by calculating the cell-to-cell similarity distance, revealing that infrared phenotypes may reflect dynamic heterogeneity changes in cell populations. This provides an alternative label-free optical approach for quantifying cellular heterogeneity and can be combined with other single-cell analysis tools to understand the genotype-to-phenotype relationship in cellular populations.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Sajjad Shirazi, Chun-Chieh Huang, Miya Kang, Yu Lu, Sriram Ravindran, Lyndon F. Cooper
Summary: The study found that modulation of miRNA biosynthesis and regulation can influence osteoblastic differentiation of human mesenchymal stem cells (hMSCs).
SCIENTIFIC REPORTS
(2021)
Review
Pharmacology & Pharmacy
Yang Zhang, Yanqi Zhong, Li Zou, Xiaoxia Liu
Summary: Well-developed placenta plays a fundamental role in successful pregnancy, while defective placental development is the underlying cause of preeclampsia. Placenta-derived mesenchymal stem cells (PMSCs) have the ability to differentiate into vessel wall cells and improve placental vasculature, artery remodeling, and immunoregulation. Recent studies have shown that preeclamptic PMSCs disrupt the balance and development in the placenta. However, transplantation or exosome therapies of PMSCs tend to improve placental vascular network and trophoblastic functions, suggesting a potential therapeutic strategy for preeclampsia.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Lingxiao Huang, Zhenni Xu, Xudan Lei, Yujun Huang, Siyu Tu, Lu Xu, Jieying Xia, Dengqun Liu
Summary: This study reveals the important role of inducible nitric oxide synthase (iNOS) in the intestinal crypts and its effects on intestinal stem cell (ISC) proliferation and differentiation. iNOS deficiency leads to morphological changes in the intestine and alters gluconeogenesis and the adaptive immune response. These findings highlight the potential of iNOS as a therapeutic target for intestinal disorders.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Ga Yeon Kim, Gyu Tae Choi, Jinryong Park, Jeongeun Lee, Jeong Tae Do
Summary: In this study, two types of porcine MSCs were compared, and it was found that adipose-derived stem cells showed better proliferation capacity, adipogenic and chondrogenic differentiation potential, and mitochondrial metabolism compared to Wharton's jelly-derived mesenchymal stem cells. This study provides insights into the characteristics of porcine MSCs and their potential applications in animal clinics and cultured meat science.
Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Review
Biochemistry & Molecular Biology
Mikhail Menshikov, Ekaterina Zubkova, Iuri Stafeev, Yelena Parfyonova
Summary: Mesenchymal stem cells are multipotent cells capable of differentiating into various cell types, and autophagy plays a crucial role in cell differentiation by maintaining cell homeostasis and facilitating phenotype changes.
Article
Multidisciplinary Sciences
Ana Sofia Rocha, Alejandro Collado-Sole, Osvaldo Grana-Castro, Jaime Redondo-Pedraza, Gonzalo Soria-Alcaide, Alex Cordero, Patricia G. Santamaria, Eva Gonzalez-Suarez
Summary: The Rank signaling pathway plays a crucial role in mammary gland homeostasis and epithelial cell differentiation. Loss of luminal Rank leads to abnormal alveolar differentiation and lactation failure, which can be restored by activating bipotency in basal cells during subsequent pregnancies.
NATURE COMMUNICATIONS
(2023)