4.7 Article

Onion quercetin monoglycosides alter microbial activity and increase antioxidant capacity

Journal

JOURNAL OF NUTRITIONAL BIOCHEMISTRY
Volume 56, Issue -, Pages 81-88

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2018.02.002

Keywords

Onion wastes; Quercetin; Antioxidant capacity; Rat; UHPLC-MS; Spireoside

Funding

  1. European Regional Development Fund under the Operational Programme Innovative Economy [POIG.02.01.00-10-171/09]

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The effects on fermentation processes in the digestive tract, the biochemical parameters and antioxidant capacity of blood in rats fed high-fat diets with quercetin (Q) and quercetin with quercetin monoglycosides (Q+MQ) preparations obtained from onion waste were evaluated. Four groups of eight animals were fed for 4 weeks with a control diet (C), a high-fat diet (HF) and high-fat diets with 0.15% addition of Q and Q+MQ preparations. HF caused an increase in alanine transaminase (ALT), non-high-density lipoprotein (non-HDL) and the atherogenic index All vs. C and a decrease in the proportion of HDL in total cholesterol (TC). Q and Q+MQ showed a tendency to moderate the values aspartate transaminase (P=.087), ALT (P<.05), TC (P=.068), non-HDL cholesterol (P<.05), triglycerides (P=.064) and the atherogenic index All (P<,05). Q+MQ significantly increased the activity of alpha-glucosidase (P<.05 vs. HF), beta-glucosidase (P<.05) and beta-galactosidase (P<.05 vs. C and Q). Q increased activity of beta-glucosidase (P<.001 vs. C and HF). Both increased the activity of beta-glucuronidase (P<.05 vs. C and HF). Both increased the antioxidant capacity of the hydrophilic fraction in serum (P<.05 vs. C and HF), and Q enhanced that of the lipid fraction (P<.001). Q preparation contained 70% quercetin, and Q+MQ preparation contained 29% quercetin and 13% quercetin monoglycosides, mainly quercetin-4'-glucoside. Both exhibited high antioxidant capacity. Supplementation with Q and Q+MQ increased the enzymatic activity of the intestinal microbiota and the antioxidant capacity of blood and revealed a tendency to improve the blood lipid profile. MQ were particularly effective in stimulating the bacterial enzymatic activity. (C) 2018 Elsevier Inc. All rights reserved.

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