Article
Biochemistry & Molecular Biology
Elsa Arcalis, Davide Mainieri, Alessandro Vitale, Eva Stoeger, Emanuela Pedrazzini
Summary: The research reveals that during transgenic Arabidopsis embryo maturation, the structure of the 16 kDa prolamin subunit transforms from dispersed fibers to compact structures resembling protein bodies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
John C. Christianson, Pedro Carvalho
Summary: This article discusses recent advances in understanding the mechanisms of ERAD and its impact on the regulation of ER functions.
Article
Multidisciplinary Sciences
Theofano Orfanelli, Spyridon Giannopoulos, Eleni Zografos, Aikaterini Athanasiou, Ann Marie Bongiovanni, Georgios Doulaveris, Tracy-Ann Moo, Dayle LaPolla, Chris N. Bakoyiannis, Georgios E. Theodoropoulos, Georgios C. Zografos, Eleni Andreopoulou, Steven S. Witkin
Summary: Peripheral blood mononuclear cells respond to malignant breast masses by increasing HSP70 production and decreasing p62 levels intracellularly. These measurements may be valuable in distinguishing between malignant and benign breast lesions in preoperative triage for women with breast masses.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Leonora Szabo, Nadia Cummins, Paolo Paganetti, Alex Odermatt, Andreas Papassotiropoulos, Celeste Karch, Jurgen Gotz, Anne Eckert, Amandine Grimm
Summary: Abnormal tau protein disrupts the interaction between the endoplasmic reticulum (ER) and mitochondria, leading to impaired mitochondrial function and cholesterol metabolism. This study shows that abnormal tau loosens the association between ER and mitochondria, particularly through the VAPB-PTPIP51 pathway. Disruption of the ER-mitochondria interaction results in altered levels of mitochondrial cholesterol and pregnenolone, which can be restored by inhibiting GSK3 beta.
Article
Cell Biology
Monica Viladomiu, Manirath Khounlotham, Belgin Dogan, Svetlana F. Lima, Ahmed Elsaadi, Emre Cardakli, Jim G. Castellanos, Charles Ng, Jeremy Herzog, Alexi A. Schoenborn, Melissa Ellermann, Bo Liu, Shiying Zhang, Ajay S. Gulati, R. Balfour Sartor, Kenneth W. Simpson, Steven M. Lipkin, Randy S. Longman
Summary: Endoplasmic reticulum stress is associated with Crohn's disease, particularly in cases with functional deficiency in protein disulfide isomerase anterior gradient 2 (AGR2). AGR2 deficiency leads to ER stress and ileocolitis, and its expression correlates with mucosal Enterobactericeae abundance. Agr2 deletion results in expansion of adherent-invasive Escherichia coli (AIEC), triggering Th17 cell ileocolitis. Mechanistically, AIEC-induced epithelial cell ER stress promotes IL-23 production by CD103(+) dendritic cells, and IL-23R is essential for ileocolitis in Agr2(-/-) mice.
Article
Medicine, Research & Experimental
Yoshiaki Morishita, Aaron P. Kellogg, Dennis Larkin, Wei Chen, Suryakiran Vadrevu, Leslie Satin, Ming Liu, Peter Arvan
Summary: This study found that acute ER stress increases CIDE-A protein levels in thyroid cells, while chronic ER stress results in lower mRNA levels of CIDE-A. Furthermore, CIDE-A mRNA response to a new acute ER stress challenge is reduced in cells surviving chronic ER stress. CIDE-A may be a novel marker associated with a noncanonical ER stress response program.
Article
Virology
Yingyi Duan, Xinhui Wang, Kehui Sun, Yuezhi Lin, Xuefeng Wang, Kewei Chen, Guangpu Yang, Xiaojun Wang, Cheng Du
Summary: This study found that SYNJ2BP can promote the production of lentiviral Env proteins by increasing the formation of MAMs and enhance the infectivity of the virus. Additionally, MAMs can provide ATP and calcium ions to the ER, reduce ER stress, and increase the production of Env.
JOURNAL OF VIROLOGY
(2022)
Article
Hematology
Malgorzata Furmanik, Rick van Gorp, Meredith Whitehead, Sadia Ahmad, Jayanta Bordoloi, Alexander Kapustin, Leon J. Schurgers, Catherine M. Shanahan
Summary: This study reveals that ER stress induces vascular calcification by increasing release of Grp78-loaded EVs, and for the first time links warfarin to promoting calcification by increasing EV release. The results of the study provide a novel mechanism of action for warfarin and the role of ER stress in cargo loading of EVs.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sanaz Dastghaib, P. Sravan Kumar, Sajjad Aftabi, Gautam Damera, Azadeh Dalvand, Adel Sepanjnia, Mohammad Kiumarsi, Mohamad-Reza Aghanoori, Sukhwinder Singh Sohal, Sudharsana R. Ande, Javad Alizadeh, Pooneh Mokarram, Saeid Ghavami, Pawan Sharma, Amir A. Zeki
Summary: Lung cells are exposed to internal and external stressors, leading to disruption of protein homeostasis and activation of the UPR. Dysregulation of the UPR is associated with disease development and various human conditions. Compounds targeting the UPR pathway show potential for future therapeutic interventions.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Yuchao Fan, Xiao Liang, Deshui Yu
Summary: The study found that low expression of SERP1 is associated with poor prognosis in patients with skin cutaneous melanoma. SERP1 level serves as an independent prognostic factor and shows significant correlation with immune cell infiltration.
Article
Medicine, General & Internal
Pavel Strnad, Mattias Mandorfer, Gourab Choudhury, William Griffiths, Christian Trautwein, Rohit Loomba, Thomas Schluep, Ting Chang, Min Yi, Bruce D. Given, James C. Hamilton, Javier San Martin, Jeffery H. Teckman
Summary: The trial demonstrated that fazirsiran treatment in patients with liver disease associated with AAT deficiency led to a significant reduction in Z-AAT concentrations in the liver and serum, as well as improvements in liver enzyme concentrations, showing potential therapeutic effects on liver fibrosis.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Richa Maheshwari, Mohammad M. Rahman, Seth Drey, Megan Onyundo, Gunar Fabig, Michael A. Q. Martinez, David Q. Matus, Thomas Mueller-Reichert, Orna Cohen-Fix
Summary: In this study, using volume electron microscopy, researchers discovered that centrosomes in the Caenorhabditis elegans early embryo are surrounded by a three-dimensional membrane reticulum called the centriculum. The formation of the centriculum depends on the presence of centrosomes and microtubules. Moreover, the centriculum affects the function of centrosomes.
Review
Biochemistry & Molecular Biology
Chuang Li, Ying Maggie Chen
Summary: The endoplasmic reticulum is crucial for protein folding, modifications, and transport, and its stress response is associated with the development of kidney diseases. Understanding the role of ER stress signaling pathways, such as IRE1, PERK, and ATF6, in the pathogenesis of kidney disease is important. Recent discovery of ER-associated biomarkers may accelerate early diagnosis and intervention in rare kidney diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Junjun Peng, Xueyan Dai, Huiqin Fan, Chenghong Xing, Yu Zhuang, Xiaona Gao, Huabin Cao, Guoliang Hu, Fan Yang
Summary: Copper (Cu) at high levels can cause harm to the host physiology, specifically resulting in nephrotoxicity, although the exact mechanism remains unclear. This study explored the relationship between mitochondria-associated ER membranes (MAM) and Cu-induced kidney mitochondrial dysfunction and ER stress. The findings revealed that excessive Cu disrupted MAM integrity, leading to MAM dysfunction, mitochondrial dysfunction, and ER stress, contributing to Cu-induced nephrotoxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Li Ma, Sirao Hai, Chenlong Wang, Chuangjiang Chen, Sajid Ur Rahman, Chang Zhao, Mansoor Ahmed Bazai, Shibin Feng, Xichun Wang
Summary: This study aimed to investigate the molecular mechanism of Zearalenone (ZEA)-induced dysfunction of mitochondria-associated endoplasmic reticulum membranes (MAM) in piglet Sertoli cells (SCs) through the endoplasmic reticulum stress (ERS) pathway. The results showed that ZEA damaged cell viability, disrupted MAM structure, and altered protein expression of various markers related to MAM function. The inhibition of ERS reduced the cytotoxicity of ZEA and restored the normal function of MAM in SCs.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Correction
Multidisciplinary Sciences
Kamil Soltysik, Yuki Ohsaki, Tsuyako Tatematsu, Jinglei Cheng, Toyoshi Fujimoto
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Kamil Soltysik, Yuki Ohsaki, Tsuyako Tatematsu, Jinglei Cheng, Toyoshi Fujimoto
NATURE COMMUNICATIONS
(2019)
Review
Cell Biology
Takuma Tsuji, Sho Takatori, Toyoshi Fujimoto
CURRENT OPINION IN CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Takafumi Kawai, Haruhiko Miyata, Hiroki Nakanishi, Souhei Sakata, Shin Morioka, Junko Sasaki, Masahiko Watanabe, Kenji Sakimura, Toyoshi Fujimoto, Takehiko Sasaki, Masahito Ikawa, Yasushi Okamura
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Ryutaro Shirakawa, Sakurako Goto-Ito, Kota Goto, Shonosuke Wakayama, Haremaru Kubo, Natsumi Sakata, Duc Anh Trinh, Atsushi Yamagata, Yusuke Sato, Hiroshi Masumoto, Jinglei Cheng, Toyoshi Fujimoto, Shuya Fukai, Hisanori Horiuchi
Article
Biochemistry & Molecular Biology
Jasmin A. Schaefer, Julia P. Schessner, Peter W. Bircham, Takuma Tsuji, Charlotta Funaya, Oliver Pajonk, Katharina Schaeff, Giulia Ruffini, Dimitrios Papagiannidis, Michael Knop, Toyoshi Fujimoto, Sebastian Schuck
Article
Biochemical Research Methods
Toshihiro Masuda, Hisaaki Hirose, Kentarou Baba, Astrid Walrant, Sandrine Sagan, Naoyuki Inagaki, Toyoshi Fujimoto, Shiroh Futaki
BIOCONJUGATE CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Kazuaki Matoba, Tetsuya Kotani, Akihisa Tsutsumi, Takuma Tsuji, Takaharu Mori, Daisuke Noshiro, Yuji Sugita, Norimichi Nomura, So Iwata, Yoshinori Ohsumi, Toyoshi Fujimoto, Hitoshi Nakatogawa, Masahide Kikkawa, Nobuo N. Noda
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Yuta Ogasawara, Jinglei Cheng, Tsuyako Tatematsu, Misaki Uchida, Omi Murase, Shogo Yoshikawa, Yuki Ohsaki, Toyoshi Fujimoto
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Kamil Soltysik, Yuki Ohsaki, Tsuyako Tatematsu, Jinglei Cheng, Asami Maeda, Shin-ya Morita, Toyoshi Fujimoto
Summary: The inner nuclear membrane of U2OS cells contains triglyceride synthesis enzymes that generate nuclear lipid droplets in situ. Inhibition of mTOR increases nuclear lipid droplets by inducing nuclear translocation of lipin-1 phosphatidic acid phosphatase. Seipin, essential for normal cytoplasmic lipid droplet formation, is absent in the inner nuclear membrane and restrains nuclear lipid droplet formation by affecting lipin-1 expression and intracellular phosphatidic acid distribution.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Minami Orii, Takuma Tsuji, Yuta Ogasawara, Toyoshi Fujimoto
Summary: The study reveals that Atg9-mediated phospholipid transport plays a crucial role in the expansion of autophagosomes. Analyses of various phospholipids distribution in autophagosomes suggest that transbilayer phospholipid movement mediated by Atg9 is involved in the biogenesis of autophagosomes.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Tetsuo Mioka, Tian Guo, Shiyao Wang, Takuma Tsuji, Takuma Kishimoto, Toyoshi Fujimoto, Kazuma Tanaka
Summary: This study discovered a stable micron-scale protein-depleted region, named the 'void zone', in yeast mutants lacking phosphatidylserine at high temperatures. The void zone excludes transmembrane proteins, certain peripheral membrane proteins, and phospholipids, while being rich in ergosterol and requiring energy and various cellular functions for its formation. Additionally, the void zones were frequently found in contact with vacuoles, forming a membrane domain at the contact site.
JOURNAL OF CELL SCIENCE
(2022)
Article
Microscopy
Hiroko Osakada, Toyoshi Fujimoto
Summary: This article presents a method for simplifying the SDS-FRL experiment by mounting freeze-fracture replicas on EM grids, reducing manual labor. This method makes it easier for more researchers to conduct SDS-FRL experiments.
Review
Cell Biology
Toyoshi Fujimoto
Summary: Lipid droplets in the cytoplasm are formed in the endoplasmic reticulum and are connected with various organelles. Nuclear lipid droplets in hepatocytes are derived from lipoprotein precursors in the ER lumen, while in non-hepatocytes and budding yeast, they are newly formed in the inner nuclear membrane. Although nuclear lipid droplets are fewer in number, their unique location suggests specific functions related to nuclear biology.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemical Research Methods
Takuma Tsuji, Toyoshi Fujimoto
Summary: This study presents a protocol for localizing newly synthesized phosphatidylcholine in yeast cells using electron microscopy, involving the application of a clickable choline analog, quick-freezing, freeze-fracture replica preparation, click chemical reaction for biotin-azide conjugation, and immunogold labeling for quantitative determination of membrane leaflet incorporation.