Review
Biochemistry & Molecular Biology
Isaac Martin, Valerie Waters, Hartmut Grasemann
Summary: The treatment of lung infection in cystic fibrosis is limited by the biofilm growth of pathogenic organisms. Interventions targeting bacteria, extracellular matrix components, and signaling pathways offer potential new therapeutic strategies to overcome current limitations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jingchun Kong, Yue Wang, Zhuocheng Yao, Yishuai Lin, Yi Zhang, Yijia Han, Tieli Zhou, Jianzhong Ye, Jianming Cao
Summary: In this study, the combination of colistin and eugenol was found to have a significant synergistic antibacterial effect against colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae. The combination increased membrane permeability and caused membrane damage, inhibiting bacteria synergistically. Eugenol was non-toxic to cells and the combination was effective in vivo. These findings suggest that the colistin/eugenol combination may be a viable treatment option for colistin-resistant bacterial infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jingchun Kong, Yue Wang, Zhuocheng Yao, Yishuai Lin, Yi Zhang, Yijia Han, Tieli Zhou, Jianzhong Ye, Jianming Cao
Summary: This study found that the combination of colistin and eugenol has a significant synergistic effect in treating colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae infections, reversing their sensitivity to colistin. Eugenol enhances membrane permeability and causes membrane damage, leading to inhibition of bacteria. The combination of colistin and eugenol is also effective in vivo. These findings suggest that the colistin/eugenol combination may be a viable treatment option for clinical infections caused by colistin-resistant pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Qi Tan, Qing Ai, Yu He, Fang Li, Jialin Yu
Summary: The ability of P.aeruginosa biofilms to activate inflammasomes and increase inflammatory response contributes to chronic infection. This study found that P.aeruginosa biofilms significantly enhance the production of IL-1β and IL-18, possibly through the regulation of NLRP3 inflammasomes rather than NLRC4 inflammasomes.
MICROBIAL PATHOGENESIS
(2022)
Article
Cell Biology
Dan Alexandru Toc, Alexandra Csapai, Florin Popa, Catalin Popa, Violeta Pascalau, Nicoleta Tosa, Alexandru Botan, Razvan Marian Mihaila, Carmen Anca Costache, Ioana Alina Colosi, Lia Monica Junie
Summary: This study describes a new, inexpensive, and easy to reproduce protocol for a 3D-printed microfluidic device for studying bacterial biofilm formation. The results obtained from the experiments show different characteristics of biofilm production between Enterococcus faecalis and Pseudomonas aeruginosa.
Review
Immunology
Veronica Roxana Flores-Vega, Silvia Yalid Vargas-Roldan, Jose Luis Lezana-Fernandez, Ricardo Lascurain, Jose Ignacio Santos-Preciado, Roberto Rosales-Reyes
Summary: Cystic fibrosis is a genetic disease caused by a mutation in the cftr gene, affecting chloride ion and water transport and leading to bacterial infections in the lungs. Autophagy plays a crucial role in pathogen clearance, but P. aeruginosa and B. cenocepacia have strategies to evade this pathway, resulting in chronic inflammatory immune responses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Microbiology
Xuejie Li, Nixuan Gu, Teng Yi Huang, Feifeng Zhong, Gongyong Peng
Summary: Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative pathogenic microorganism known for its virulence factors, biofilm forming capability, and antimicrobial resistance. Despite being underestimated, it is a foodborne pathogen that can be found in various food groups. Chemical preservatives used to suppress microbial growth in food can pose food safety concerns. Therefore, finding effective and natural alternative antimicrobial agents for food processing is crucial. This review focuses on recent advances in food safety related to P. aeruginosa, including antimicrobial resistance, virulence factors, and prevention measures.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biophysics
Wing-Hin Lee, Ramin Rohanizadeh, Ching-Yee Loo
Summary: This study developed a novel bioactive bone substitute with improved anti-biofilm activity by functionalizing with curcumin, which effectively inhibits biofilm formation and controls viable planktonic cells. Curcumin was successfully incorporated onto HA and sustainably released over 14 days, showing stronger anti-biofilm effect against Staphylococcus aureus. The curcumin-loaded HA composite could be an alternative antimicrobial agent to control the risk of infections in postsurgical implants.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Giovanni Di Bonaventura, Veronica Lupetti, Simone De Fabritiis, Alessandra Piccirilli, Annamaria Porreca, Marta Di Nicola, Arianna Pompilio
Summary: This study evaluated the antibacterial and antivirulence activities of eight FDA-approved non-antibiotic drugs and their effects on biofilm formation. Ciclopirox and actinomycin D showed the best antibacterial activity and were most effective against preformed biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Kara R. Eichelberger, Saikat Paul, Brian M. Peters, James E. Cassat
Summary: This review highlights the impact of Candida albicans-bacteria interactions on healthy polymicrobial community structure, host immune responses, and microbial pathogenesis, and discusses the significance of these interactions in discovering novel antivirulence compounds for fungal infections.
TRENDS IN MICROBIOLOGY
(2023)
Review
Immunology
Qiyao Chai, Zehui Lei, Cui Hua Liu
Summary: Pyroptosis is a proinflammatory form of programmed cell death characterized by membrane pore formation and release of intracellular inflammatory mediators. Bacterial pathogens have developed mechanisms to regulate host pyroptosis for evading immune clearance and establishing progressive infection. Understanding the regulatory mechanisms of pyroptosis in host-bacterial interactions can provide insights into the pathogenesis of infectious diseases and aid in the development of therapeutic strategies against bacterial pathogens.
SEMINARS IN IMMUNOLOGY
(2023)
Article
Virology
Elisabed Zaldastanishvili, Lika Leshkasheli, Mariam Dadiani, Lia Nadareishvili, Lia Askilashvili, Nino Kvatadze, Marina Goderdzishvili, Mzia Kutateladze, Nana Balarjishvili
Summary: This retrospective study focused on three patients who underwent phage therapy at EPTC in Georgia, revealing that some pathogens persisted through phage therapy, indicating potential adaptation strategies.
Article
Pharmacology & Pharmacy
F. Alghofaili
Summary: This study discovered that sterile crude supernatants extracted from the cultures of Salmonella enterica and Pseudomonas aeruginosa significantly inhibited biofilm formation of Pseudomonas aeruginosa and Kiebsiella pneumoniae, respectively. The active agents in the culture supernatants responsible for biofilm inhibition have not been determined yet. Further research should be conducted to explore the therapeutic potential of culture supernatants of Salmonella enterica and Pseudomonas aeruginosa for reducing biofilm formation caused by bacteria causing nosocomial infections.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2022)
Article
Microbiology
Giovanni Di Bonaventura, Veronica Lupetti, Fabio Verginelli, Sara Giancristofaro, Rosemary Barbieri, Giovanni Gherardi, Arianna Pompilio
Summary: This study evaluated the antibacterial, antibiofilm, and antivirulence activities of apramycin compared to tobramycin against P. aeruginosa in cystic fibrosis patients. Apramycin showed better efficacy than tobramycin, without cytotoxicity to bronchial epithelial CF cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Adeline Peignier, Dane Parker
Summary: This review discusses recent literature that provides new insights into the mechanisms of how type I IFN signaling exerts its effects on the outcome of infection from the host's point of view. The identified mechanisms have been wide and varied between pathogens.
TRENDS IN MICROBIOLOGY
(2021)