Article
Microbiology
Parsa Ghadermazi, Angela Re, Luca Ricci, Siu Hung Joshua Chan
Summary: Gas fermentation is a promising method to convert low-cost waste gases into valuable chemicals. This study used modeling and kinetic ensemble modeling to analyze metabolic engineering interventions for increased 2,3-butanediol production and reduced carbon loss. The results suggested that interventions targeting by-products branching from acetyl-CoA and pyruvate are effective strategies.
Article
Biotechnology & Applied Microbiology
Mengyun Kou, Zhenzhen Cui, Jing Fu, Wei Dai, Zhiwen Wang, Tao Chen
Summary: In this study, a C. glutamicum strain was engineered to efficiently produce optically pure (2R,3R)-2,3-butanediol. Through genetic modification and fermentation engineering, the titer of (2R,3R)-2,3-butanediol reached a record-high level, making C. glutamicum a competitive candidate for commercial production of this compound.
MICROBIAL CELL FACTORIES
(2022)
Review
Biotechnology & Applied Microbiology
Jae Won Lee, Ye-Gi Lee, Yong-Su Jin, Christopher V. Rao
Summary: 2,3-Butanediol (2,3-BDO) is a promising commodity chemical with various industrial applications. Fermentation-based production of 2,3-BDO is considered a more sustainable alternative to petroleum-based chemical processes. Metabolic engineering strategies have been developed to improve the production of 2,3-BDO from inexpensive sugars by non-pathogenic microorganisms.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chan Woo Song, Chelladurai Rathnasingh, Hyohak Song
Summary: This study demonstrates the development of non-mucoid and byproduct-free strains of Bacillus licheniformis for efficient synthesis of 2,3-butanediol. By deleting specific genes and optimizing culture conditions, the goal of high yield and minimal byproducts was achieved successfully.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemical Research Methods
Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: Efforts to improve the production efficiency of (R)-1,3-BDO by Escherichia coli through different metabolic engineering strategies have successfully optimized key pathway enzymes, increased NADPH supply, optimized fermentation conditions, and reduced byproducts formation. The best engineered strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theoretical yield. Additionally, the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate has been demonstrated.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Daniel Tinoco, Lucy Seldin, Paulo Luiz de Andrade Coutinho, Denise Maria Guimaraes Freire
Summary: Optimization of culture conditions is an effective strategy to improve bio-based 2,3-butanediol (2,3-BDO) production. In this study, a new and safe strain Paenibacillus peoriae NRRL BD-62 was used to achieve a 2,3-BDO yield of 90% in batch cultures at specific conditions. In fed-batch fermentation, a yield of 39.4 g/L of 2,3-BDO was obtained by adjusting the operational limitations and utilizing acetoin, lactic acid, and ethanol as substrates.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Article
Agronomy
Jiahui Sun, Xuejing Xu, Yannan Wu, Huili Sun, Guodong Luan, Xuefeng Lu
Summary: In this study, we engineered Synechocystis sp. PCC 6803 to synthesize valencene, a valuable ingredient in the food and cosmetics industries. By introducing a heterologous valencene synthase and modifying the native MEP pathway, we obtained an efficient cyanobacterial cell factory that produced 154 mg/L valencene during a semi-continual cultivation with robust growth and production in non-sterilized conditions. We also achieved the production of other sesquiterpenes, showing promising potentials as a universal chassis.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2023)
Review
Biotechnology & Applied Microbiology
Sofia Maina, Ashish A. Prabhu, Narisetty Vivek, Anestis Vlysidis, Apostolis Koutinas, Vinod Kumar
Summary: This review summarizes the applications of bio-based platform chemicals 2,3-butanediol (BDO) and acetoin in various industries, as well as the novel technological developments in genetic and metabolic engineering strategies for their production. The review also discusses the valorization of renewable feedstocks and the development of bioprocesses for the production of BDO and acetoin. Techno-economic aspects evaluating the viability and industrial potential of bio-based BDO production are presented.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Wensi Meng, Yongjia Zhang, Liting Ma, Chuanjuan Lu, Ping Xu, Cuiqing Ma, Chao Gao
Summary: The feasibility of non-sterilized fermentation using Vibrio natriegens as a microbial chassis and producing chemicals through metabolic engineering has been confirmed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Guangxin Huo, Maria R. Foulquie-Moreno, Johan M. Thevelein
Summary: The engineered microbial cell factories showed high production of 2,3-butanediol with the highest reported titer and close to the maximum theoretical yield, making them suitable for industrial implementation.
MICROBIAL CELL FACTORIES
(2022)
Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Vivek Kumar Gaur, Junhak Lee, Sunghoon Park
Summary: The production of 1,3-butanediol from glucose was achieved by engineering a pathway in Escherichia coli and optimizing gene expression levels and culture conditions. The highest ever reported titer of 1,3-butanediol in biological production methods was achieved at 257 mM in 36 hours with a yield of 0.51 mol/mol. This study provides a promising strategy for sustainable synthesis of other acetyl-CoA-derived chemicals using microbial cell factories.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Keisuke Morita, Taisuke Seike, Jun Ishii, Fumio Matsuda, Hiroshi Shimizu
Summary: In this study, dCas9 was used to reduce ethanol synthesis and enhance 2,3-butanediol production without affecting glucose. By controlling the expression of PDC1, ethanol production was successfully suppressed, while the production of the target product, 2,3-butanediol, was increased.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Roopa Parate, Mrunal Borgave, Mahesh Dharne, Chandrashekhar Rode
Summary: The study demonstrates the concept of a 'Circular Economy' by converting bioglycerol with a cell-free extract of Enterobacter aerogenes, resulting in the production of 2,3-butanediol. This method not only improves the overall economics of the process, but also effectively utilizes the remaining cell-free extract.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Tayyab Islam, Thuan Phu Nguyen-Vo, Seunghyun Cho, Junhak Lee, Vivek Kumar Gaur, Sunghoon Park
Summary: This study focuses on metabolic engineering of Escherichia coli to efficiently produce 1,3-BDO. By reducing the accumulation of intermediate metabolites and byproducts and improving NADPH availability, a record-high titer of optically pure (R)-1,3-BDO was achieved.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Hung Li, Nam Ngoc Pham, Claire R. Shen, Chin-Wei Chang, Yi Tu, Yi-Hao Chang, Jui Tu, Mai Thanh Thi Nguyen, Yu-Chen Hu
Summary: In this study, a combinatorial CRISPR interference (CRISPRi) library strategy was used to enhance cyanobacterial 2,3-butanediol (2,3-BDO) production by regulating specific genes. The results showed that inhibiting certain genes can increase 2,3-BDO synthesis, with fbp and pdh suppression having a more significant effect. This method opens up new possibilities for metabolic engineering of cyanobacteria.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hye Jin Lim, Kyung-Ho Lee, Dong-Myung Kim
BIOCHEMICAL ENGINEERING JOURNAL
(2018)
Article
Plant Sciences
Soo-Jin Yeom, Moonjung Kim, Seong Keun Kim, Dae-Hee Lee, Kil Koang Kwon, Hyewon Lee, Haseong Kim, Dong-Myung Kim, Seung-Goo Lee
Article
Agriculture, Multidisciplinary
Zahra Zahra, Seok-Young Kim, Hye-Youn Kim, Hwanhui Lee, Heayyean Lee, Jun-Yeon Jeon, Dong-Min Kim, Dong-Myung Kim, Seong-Joo Hong, Byung-Kwan Cho, Hookeun Lee, Choul-Gyun Lee, Muhammad Arshad, Hyung-Kyoon Choi
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2018)
Article
Biotechnology & Applied Microbiology
Seo-Young Go, Kyung-Ho Lee, Dong-Myung Kim
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2018)
Article
Multidisciplinary Sciences
Soo-Jin Yeom, Moonjeong Kim, Kil Koang Kwon, Yaoyao Fu, Eugene Rha, Sung-Hyun Park, Hyewon Lee, Haseong Kim, Dae-Hee Lee, Dong-Myung Kim, Seung-Goo Lee
NATURE COMMUNICATIONS
(2018)
Article
Chemistry, Multidisciplinary
Ju-Young Byun, Kyung-Ho Lee, Yong-Beom Shin, Dong-Myung Kim
Article
Chemistry, Analytical
Yeon-Jae Jang, Kyung-Ho Lee, Tae Hyeon Yoo, Dong-Myung Kim
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Hyeon-Jung Yang, Kyung-Ho Lee, Hye Jin Lim, Dong-Myung Kim
BIOTECHNOLOGY JOURNAL
(2019)
Article
Chemistry, Multidisciplinary
Byeong-Jin Moon, Kyung-Ho Lee, Dong-Myung Kim
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2019)
Review
Pharmacology & Pharmacy
Kyung-Ho Lee, Dong-Myung Kim
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Hyewon Lee, Ji In Baek, Su Jin Kim, Kil Koang Kwon, Eugene Rha, Soo-Jin Yeom, Haseong Kim, Dae-Hee Lee, Dong-Myung Kim, Seung-Goo Lee
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Engineering, Chemical
So Jeong Lee, Ho-Cheol Kim, Dong-Myung Kim
KOREAN CHEMICAL ENGINEERING RESEARCH
(2019)
Article
Chemistry, Analytical
Yeon-Jae Jang, Kyung-Ho Lee, Tae Hyeon Yoo, Dong-Myung Kim
ANALYTICAL CHEMISTRY
(2017)
Review
Microbiology
Kyung-Ho Lee, Dong-Myung Kim
FEMS MICROBIOLOGY LETTERS
(2018)