Article
Chemistry, Analytical
Yongjuan Jia, Xingli Liu, Li Xu, Jinbao Zhao, Junjun Ni, Yuanyuan Zhang
Summary: A high-throughput solid-phase extraction method was developed for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography-tandem mass spectrometry in this study. The method showed good linearities and sensitivity for each steroid hormone within the quantitative range. It has been successfully applied to series of samples from clinical trials with no significant difference between serum and ethylenediaminetetraacetic acid plasma samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemical Research Methods
Lili Shao, Xi Chen, Jinxiu Lyu, Meng Zhao, Qing Li, Shuai Ji, Qiang Sun, Daoquan Tang, Houfa Geng, Mengzhe Guo
Summary: Researchers have developed a novel material for the enrichment of specific compounds related to thyroid diseases, which can aid in the diagnosis of thyroid cancer. Through this method, they found that certain T2 thyroid hormones are significantly elevated in patients with thyroid cancer compared to healthy individuals.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Jufang Li, Aiping Xu, Jianyou Xue, Wei Qian, Ping Xu, Zhineng Hu, Chen Chen, Chaochao Wu
Summary: The paper proposes a novel method of deep eutectic solvent (DES)-assisted liquid-liquid extraction (LLE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for extracting steroid hormones from serum. The method has good linear calibration ranges, high extraction recoveries, and low limits of quantification, making it a simple and environmentally-friendly method.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Kohki Takaguchi, Sumika Ono, Rumi Tanoue, Tatsuya Kunisue, Shinsuke Tanabe, Kei Nomiyama
Summary: Thyroid hormones play a critical role in regulating biological processes, and determination of deiodinated TH metabolites in key organs can help understand tissue-specific TH metabolism and homeostasis. A highly sensitive method was developed to determine six THs in dog brain and liver, which was successfully applied to analyze THs in dogs exposed to PCBs and revealed alterations in peripheral TH metabolism caused by environmental pollutants.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Songlin Yu, Yutong Zou, Yicong Yin, Jialei Yu, Qianqian Li, Shaowei Xie, Wei Luo, Xiaoli Ma, Danchen Wang, Ling Qiu
Summary: A robust LC-MS/MS method for the simultaneous determination of seven androgens in plasma samples was successfully established and validated, which plays important roles in clinical application.
Article
Chemistry, Multidisciplinary
Meng-Yuan Chen, Chu-Bo Qi, Xiao-Meng Tang, Jiang-Hui Ding, Bi-Feng Yuan, Yu-Qi Feng
Summary: This study comprehensively analyzed the alterations of RNA modifications in different RNA species in thyroid carcinoma using liquid chromatography-tandem mass spectrometry. The altered patterns of RNA modifications may serve as indicators of thyroid carcinoma.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Shengnan Ji, Xiaomeng Liu, Jing Ha, Lianfeng Ai, Zheng Li
Summary: A sensitive and simple LC-MS/MS method was developed for the determination of orelabrutinib in human plasma and cerebrospinal fluid. The method involved simple protein precipitation, efficient separations, and optimized mass spectrometric conditions to increase selectivity and sensitivity. The method was validated for accuracy, precision, selectivity, linearity, recovery, matrix effect, stability, and limits of quantification. It exhibited a lower limit of quantification of 0.50 ng/mL, with intraday and interday precision RSD both less than 15%, and a recovery rate of 85.7%-92.9%. The method was successfully applied for quantitation in clinical patients treated with orelabrutinib.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Fatemeh Aghai, Sebastian Zimmermann, Max Kurlbaum, Pius Jung, Theo Pelzer, Hartwig Klinker, Nora Isberner, Oliver Scherf-Clavel
Summary: A liquid chromatography tandem mass spectrometry method has been developed and validated for the analysis of ten kinase inhibitors in human serum and plasma, meeting the validation guidelines of regulatory agencies. The method allows for rapid and sensitive monitoring of kinase inhibitor concentrations in patients.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Masaki Takiwaki, Fumio Nomura, Mamoru Satoh, Sachio Tsuchida, Kazuo Otake, Junko Takagi
Summary: This study aimed to develop an LC/MS/MS-based method for detecting human plasma AVP and compare it with the traditional RIA method. Although there was a weak overall correlation between the results obtained using the two different methods, disagreements were observed at low concentrations.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Medical Laboratory Technology
Jenny Yeuk-Ki Cheng, Felix Chi-Kin Wong, Edith Wing-Kar Chow, Wendy Wan-Hang Lau, Kitty Kit-Ting Cheung, Timothy Hua-Tse Cheng, Teresa Kam-Chi Tsui, Alan Shek-Lun Chan, Clara Wai-Shan Lo, Chung-Shun Ho
Summary: This study provides age-specific LC-MS/MS reference intervals for PRA and PAC in both normotensive and EH populations for local Chinese in Hong Kong.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Songlin Yu, Danchen Wang, Xiaoli Ma, Yuanyuan Zhang, Dandan Sun, Yutong Zou, Jialei Yu, Fang Zhang, Guohua Yang, Shaowei Xie, Qian Cheng, Xinqi Cheng, Ling Qiu
Summary: A LC-MS/MS method was established to quantify G17 and G34 simultaneously, showing high analytical performance. The method was compared with CLIA and RIA, and it was found to be highly correlated with both methods. The study suggests that G17 may be the most specific biomarker for gastrinoma diagnosis.
CLINICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Patrik Matlak, Hana Brozmanova, Pavel Sistik, Ivana Kacirova, Pavel Hradilek, Milan Grundmann
Summary: This study aimed to develop a LC-MS/MS method for quantifying natalizumab in human serum and cerebrospinal fluid (CSF) and applying it to clinical settings. By analyzing specific peptide sequences of natalizumab, the concentration was successfully determined. The method met all accuracy and precision acceptance criteria according to the EMA guideline and is suitable for clinical applications.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Mateus Araujo Castro e Souza, Naialy Fernandes Araujo Reis, Isabella Cristina Placido de Oliveira Pacheco, Maria Auxiliadora Parreiras Martins, Maria Beatriz A. Gloria, Gerson Antonio Pianetti, Christian Fernandes
Summary: This study developed a modern method for the simultaneous determination of eight cardiovascular drugs in human plasma. The method is simple, fast, environmentally friendly, and has high accuracy and reliability.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Yu Ra Lee, Ji Won Lee, Jongki Hong, Bong Chul Chung
Summary: A simultaneous quantitative profiling method was developed and validated for polyamines and steroids using liquid chromatography-tandem mass spectrometry. The method showed differences in levels between breast cancer patients and controls, with certain metabolites significantly higher in the patient group. This mass spectrometry-based method is applicable for investigating breast cancer, androgen-dependent diseases, and polyamine-related diseases.
Article
Environmental Sciences
Prasun Goswami, Keerthi S. Guruge, Rumi Tanoue, Yukino A. Tamamura, K. B. S. N. Jinadasa, Kei Nomiyama, Tatsuya Kunisue, Shinsuke Tanabe
Summary: This study investigated the presence of pharmaceutically active compounds (PACs) in wastewater from hospitals and adjacent surface waters in Sri Lanka, revealing variations in removal rates among facilities and potential ecological risks posed by PACs to aquatic organisms. The findings suggest ineffective treatment processes and increased PAC concentrations in effluents, highlighting the importance of monitoring and addressing pharmaceutical pollution in water bodies.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2022)
Article
Biochemical Research Methods
Kohki Takaguchi, Sumika Ono, Rumi Tanoue, Tatsuya Kunisue, Shinsuke Tanabe, Kei Nomiyama
Summary: Thyroid hormones play a critical role in regulating biological processes, and determination of deiodinated TH metabolites in key organs can help understand tissue-specific TH metabolism and homeostasis. A highly sensitive method was developed to determine six THs in dog brain and liver, which was successfully applied to analyze THs in dogs exposed to PCBs and revealed alterations in peripheral TH metabolism caused by environmental pollutants.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Environmental Sciences
Thant Zin Tun, Tatsuya Kunisue, Shinsuke Tanabe, Maricar Prudente, Annamalai Subramanian, Agus Sudaryanto, Pham Hung Viet, Haruhiko Nakata
Summary: The study revealed a high prevalence of microplastics in soils from open-dumping sites in Asian countries, primarily originating from everyday plastic products such as polyethylene and polypropylene. Furthermore, phthalates and antioxidants found in the floating fraction accounted for a significant proportion of the total additives in soils, indicating that approximately half of the additives in dumping site soils were derived from microplastics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Correction
Environmental Sciences
Vahab Vaezzadeh, Margaret William Thomes, Tatsuya Kunisue, Nguyen Minh Tue, Gan Zhang, Mohamad Pauzi Zakaria, Yang Amri Affendi, Fook Choy Yap, Li Lee Chew, Hong Wooi Teoh, Choon Weng Lee, Chui Wei Bong
Review
Engineering, Environmental
Anh Quoc Hoang, Nguyen Minh Tue, Minh Binh Tu, Go Suzuki, Hidenori Matsukami, Le Huu Tuyen, Pham Hung Viet, Tatsuya Kunisue, Shin-ichi Sakai, Shin Takahashi
Summary: This review paper comprehensively discusses the management and research aspects of e-waste in an Asian developing country. The lack of effective management and recycling systems in Vietnam has resulted in localized contamination and human exposure to toxic chemicals. Primitive processing activities have been identified as significant contributors to environmental emissions and human exposure to toxic elements and pollutants. Informal e-waste processing releases pollutants at similar levels as large-scale facilities in developed countries.
ENVIRONMENTAL GEOCHEMISTRY AND HEALTH
(2023)
Article
Environmental Sciences
Anh Quoc Hoang, Ryogo Karyu, Nguyen Minh Tue, Akitoshi Goto, Le Huu Tuyen, Hidenori Matsukami, Go Suzuki, Shin Takahashi, Pham Hung Viet, Tatsuya Kunisue
Summary: The co-occurrence of halogenated flame retardants (HFRs) and organophosphate esters (OPEs) in the environment of informal waste processing areas in Vietnam was investigated in this study. The results showed higher levels of HFRs and OPEs in the e-waste recycling workshops compared to the vehicle processing workshops, suggesting different usage patterns of these substances in different industries. The human health risk related to HFRs and OPEs in settled dust was found to be low.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Nguyen Minh Tue, Hidenori Matsukami, Le Huu Tuyen, Go Suzuki, Pham Hung Viet, Agus Sudaryanto, Annamalai Subramanian, Shinsuke Tanabe, Tatsuya Kunisue
Summary: This study evaluated the receptor agonist activities of estrogen, androgen, and glucocorticoid in river water samples from Chennai and Bangalore (India), Jakarta (Indonesia), and Hanoi (Vietnam) using CALUX assays. The results showed that the major contributors to the agonist activities were estrogens and androgens, with the samples from India having higher activities.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Hidenori Matsukami, Tatiya Wannomai, Natsuyo Uchida, Nguyen Minh Tue, Anh Quoc Hoang, Le Huu Tuyen, Pham Hung Viet, Shin Takahashi, Tatsuya Kunisue, Go Suzuki
Summary: Measuring personal exposure to flame retardants (FRs) is crucial for assessing and controlling human health risks posed by FRs during the recycling of electronic waste (e-waste) and end-of-life vehicles (ELVs). The study examined the use of handwipes and silicone wristbands to measure personal FR exposure for e-waste and ELV recycling workers and their children in Vietnam. The results showed that both handwipes and wristbands can effectively measure FR exposure, and similar FR profiles were found in both workers and their children.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Kazusa Nozaki, Rumi Tanoue, Tatsuya Kunisue, Nguyen Minh Tue, Sadahiko Fujii, Nao Sudo, Tomohiko Isobe, Kei Nakayama, Agus Sudaryanto, Annamalai Subramanian, Keshav A. Bulbule, Peethambaram Parthasarathy, Le Huu Tuyen, Pham Hung Viet, Masakazu Kondo, Shinsuke Tanabe, Kei Nomiyama
Summary: In Asian developing countries, undeveloped and ineffective sewer systems are causing surface water pollution by pharmaceuticals and personal care products (PPCPs), posing risks to freshwater fauna. This study aimed to assess the contamination status, bio-accumulation, and potential risks of PPCP residues in surface water and freshwater fish from three Asian countries. Results showed that bioaccumulation factors measured in fish and water samples were in close agreement for the majority of compounds, but certain species-specific differences were observed. The presence of certain PPCPs in fish plasma and surface water indicated potential adverse effects on wild fish and high ecological risks on Asian freshwater ecosystems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
