Article
Chemistry, Analytical
Yunhan Yu, Mengya Kuang, Baohua Zheng, Meiling Wang, Zhenqiang Liu, Huilan Xu, Jianwu Wang
Summary: An improved method for detecting endocrine-disrupting chemicals in milk using high performance liquid chromatography tandem mass spectrometry is presented. The method is easy, fast, and cost-efficient, and provides a reference for the detection of various endocrine disruptors in milk and other dairy products.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Food Science & Technology
Thammaporn Junsai, Saranya Poapolathep, Samak Sutjarit, Mario Giorgi, Zhaowei Zhang, Antonio Francesco Logrieco, Peiwu Li, Amnart Poapolathep
Summary: This study investigated the occurrence of multi-mycotoxins in six types of vegetable oils available in Thailand, revealing variations in mycotoxin contamination among different oil samples. The levels of analyzed mycotoxins were found to be below regulatory limits, indicating a low health risk to consumers.
Article
Engineering, Chemical
Chang-Seob Seo, Mee-Young Lee
Summary: Insamyangpye decoction (ISYPD) is a complex herbal prescription used in Korea for lung-related diseases, consisting of 13 herbal medicines. A liquid chromatography tandem mass spectrometry method based on multiple reaction monitoring (MRM) was developed for quality assessment of ISYPD, quantifying 10 marker components for accurate and sensitive analysis. Marker components were found at concentrations ranging from 0.09 to 7.47 mg/g.
Article
Food Science & Technology
Xin Guan, Yuchao Feng, Decheng Suo, Zhiming Xiao, Shi Wang, Ying Liang, Xia Fan
Summary: In this study, a method for detecting 11 mycotoxins in maize was established using multiple-impurity adsorption purification (MIA) technologies and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The validated method was suitable for simultaneous determination of multiple mycotoxins in maize.
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Patrik Matlak, Hana Brozmanova, Pavel Sistik, Ivana Kacirova, Pavel Hradilek, Milan Grundmann
Summary: This study aimed to develop a LC-MS/MS method for quantifying natalizumab in human serum and cerebrospinal fluid (CSF) and applying it to clinical settings. By analyzing specific peptide sequences of natalizumab, the concentration was successfully determined. The method met all accuracy and precision acceptance criteria according to the EMA guideline and is suitable for clinical applications.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Analytical
Veronika Pesakova, Hana Brozmanova, Pavel Sistik, Zuzana Kusnirikova, Ivana Kacirova, Milan Grundmann
Summary: A rapid and simple method was developed to determine the total and free concentration of Teriflunomide in the serum of patients with multiple sclerosis. The method showed high sensitivity and can be applied to clinical samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Polymer Science
Andrea Schweighuber, Alexander Felgel-Farnholz, Thomas Boegl, Joerg Fischer, Wolfgang Buchberger
Summary: The importance of mechanical recycling of polymers has increased in recent decades due to government regulations worldwide. The increased thermo-mechanical stress during the recycling process can significantly impact the quality of recyclates. Research has shown the occurrence of odd-numbered linear alkane chains in polyethylene samples and oxidized branched alkanes in polypropylene samples during the recycling process.
POLYMER DEGRADATION AND STABILITY
(2021)
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Biochemical Research Methods
Shuailong Chen, Xuan Li, Shijia Liu, Longshan Zhao, Wenyong Zhang, Zhili Xiong, Hemi Luan
Summary: A rapid, sensitive, and high-throughput isotope dilution liquid chromatography-negative ion electrospray tandem mass spectrometry (IDLC-nESI-MS/MS) method was developed for accurate quantification of multiple ceramides. This method demonstrated good linearity and recovery, and could be applied for studying ceramide biology and disease diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Ryan W. Pearce, Jillian Kodger, Yana Sandlers
Summary: This study developed an LC-ESI-MS/MS method for analyzing various cellular acyl-CoA in a single injection, with a quick extraction step and compensation for matrix effects through isotopically labeled internal standards and matrix-matched calibration. The method was successfully applied to identify 21 cellular acyl-CoA in SK-HEP-1 cells and investigate alterations in acyl-CoA levels post Mito Q antioxidant intervention.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Seunghwa Lee, Kang Mi Lee, Sang Moon Han, Hyeon-Jeong Lee, Changmin Sung, Hophil Min, Hosub Im, Sang Beom Han, Sangwon Cha, Jaeick Lee
Summary: To assess the combined effects of environmental pollutants (EPOLs), a comprehensive analytical method was developed to simultaneously analyze multiple EPOLs. The method overcame technical challenges associated with enzyme hydrolysis, simultaneous extraction, and analysis. The method was validated and showed potential for long-term, large-scale, and data-rich human biomonitoring of EPOLs.
ENVIRONMENTAL RESEARCH
(2022)