Article
Biochemistry & Molecular Biology
Corinna Lieleg, Ana Novacic, Sanja Musladin, Andrea Schmid, Goezde Gueclueler Akpinar, Slobodan Barbaric, Philipp Korber
Summary: Chromatin remodeling by ATP-dependent enzymes is crucial for genomic processes. The recruitment of remodelers, such as the SWI/SNF complex, in the removal of nucleosomes in yeast PHO gene induction is specific and may affect the outcome of remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Satoru Tsunemine, Hiromi Nakagawa, Yutaka Suzuki, Yota Murakami
Summary: This study reveals the crucial role of chromatin remodeler RSC in the establishment and maintenance of distinct chromatin domains, particularly centromeres, in eukaryotic cells. RSC prevents the spreading of CENP-A(Cnp1) into heterochromatin and controls its distribution by decompacting the chromatin structure.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Developmental Biology
Rodrigo O. de Castro, Luciana Previato de Almeida, Agustin Carbajal, Irma Gryniuk, Roberto J. Pezza
Summary: Gametogenesis in mammals involves regulated developmental transitions and changes in gene transcription. SWI/SNF chromatin remodelers are involved in gene transcription and DNA repair, but their role in meiosis is not well understood. Conditional knockout mice for ARID2, a regulatory subunit of PBAF, were generated and compared with BRG1 knockout mice. ARID2 activity is required at the end of prophase I, while BRG1 acts at an early stage of meiosis. Defects in spindle assembly and chromosome-spindle attachment in ARID2 knockout mice are attributed to an increase in aurora B kinase at centromeres. Different PBAF complexes regulate different stages of meiosis and gametogenesis.
Article
Biochemistry & Molecular Biology
Yulii V. Shidlovskii, Oleg V. Bylino, Alexander V. Shaposhnikov, Zaur M. Kachaev, Lyubov A. Lebedeva, Valeria V. Kolesnik, Diego Amendola, Giovanna De Simone, Nadia Formicola, Paul Schedl, Filomena Anna Digilio, Ennio Giordano
Summary: The chromatin remodeler SWI/SNF plays a key role in gene activation by opening chromatin structure on promoters and enhancers. A study on Drosophila showed that SWI/SNF factors, specifically the BAP170 and SAYP subunits of the PBAP subfamily, mediate enhancer-dependent transcription of a reporter gene. This suggests that SWI/SNF and its subunits are critical for establishing enhancer-mediated transcription.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Teresita Padilla-Benavides, Monserrat Olea-Flores, Tapan Sharma, Sabriya A. Syed, Hanna Witwicka, Miriam D. Zuniga-Eulogio, Kexin Zhang, Napoleon Navarro-Tito, Anthony N. Imbalzano
Summary: Mammalian SWI/SNF (mSWI/SNF) complexes are vital ATP-dependent chromatin remodeling enzymes for normal cellular functions. They consist of three sub-families, namely BAF, ncBAF, and PBAF, based on the presence of specific subunit proteins. This study investigated the role of these sub-families in myoblast differentiation by knocking down the genes encoding their unique subunit proteins. The results revealed that the BAF complex and Baf250A were necessary for lineage-specific gene expression, while Brd9 and the ncBAF complex indirectly contributed to differentiation. Baf180 was found to be dispensable for myoblast differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Ye Hu, Xin Wang, Jiaying Song, Jiacheng Wu, Jia Xu, Yangyang Chai, Yuanyuan Ding, Bingjing Wang, Chunmei Wang, Yong Zhao, Zhongyang Shen, Xiaoqing Xu, Xuetao Cao
Summary: The transcription factor IRF3 plays a critical role in inducing antiviral type I interferon (IFN-I) production. The epigenetic remodelers ARID1A and NSD2 have been identified as key regulators in promoting IFN-I expression by enhancing chromatin accessibility of IRF3 at the Ifn-I promoters. This study provides insight into the crosstalk between chromatin remodeling, histone modification, and transcription factors in the epigenetic regulation of antiviral innate immunity.
CELL DEATH & DISEASE
(2021)
Review
Plant Sciences
Tomasz Bieluszewski, Sandhan Prakash, Thomas Roule, Doris Wagner
Summary: SWI/SNF class chromatin remodeling complexes play crucial roles in facilitating access of proteins to the genomic DNA and altering chromatin compaction and accessibility. Recent studies have identified different subtypes of SWI/SNF complexes with unique properties and functions. Understanding the complex regulation of SWI/SNF activity and its impact on proper development and response to extrinsic cues is of great importance.
ANNUAL REVIEW OF PLANT BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Sophie M. Navickas, Katherine A. Giles, Kate H. Brettingham-Moore, Phillippa C. Taberlay
Summary: The chromatin remodeler SMARCA4/BRG1 plays a key role in brain tumour development, with its function varying in different tumour types and subtypes. Altered SMARCA4 expression is associated with various brain tumours, and mutations primarily occur in the catalytic ATPase domain, which has tumour suppressor activity. However, SMARCA4 can also promote tumourigenesis through overexpression. This review explores the complex interaction between SMARCA4 and different brain cancer types, highlighting its role in tumour pathogenesis, regulated pathways, and advances in understanding the functional relevance of mutations. The potential of targeting SMARCA4 as adjuvant therapy to enhance current brain cancer treatment methods is also discussed.
Review
Biochemistry & Molecular Biology
Cedric R. Clapier
Summary: The establishment and maintenance of genome packaging into chromatin is crucial for defining cellular identity and function. Dynamic regulation of chromatin organization and nucleosome positioning, carried out by remodelers, plays a key role in DNA transactions and gene expression regulation. Alterations in remodelers can introduce dissonance into the conversations with nucleosomes, modifying chromatin organization and contributing to oncogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Genetics & Heredity
Sai Gourisankar, Andrey Krokhotin, Wendy Wenderski, Gerald R. Crabtree
Summary: Recent genetic studies have shown that chromatin remodellers exhibit remarkable biological specificity and dosage sensitivity. They are associated with human developmental disorders and cancers, and researchers are investigating their specific functions. Structural and biochemical studies are uncovering critical interactions between remodelling complex subunits, nucleosomes, and other transcriptional regulators, while in vivo analysis provides insights into their dynamics.
NATURE REVIEWS GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Saumya M. De Silva, Alisha Dhiman, Surbhi Sood, Kilsia F. Mercedes, William J. Simmons, Morkos A. Henen, Beat Vogeli, Emily C. Dykhuizen, Catherine A. Musselman
Summary: PBRM1 is a subunit of the PBAF chromatin remodeling complex and is frequently mutated in clear cell renal cell carcinoma patients. It functions as a chromatin binding subunit and contains six tandem bromodomains which can bind nucleosomes acetylated at histone H3 lysine 14 (H3K14ac). In addition, the second and fourth bromodomains of PBRM1 also have the ability to bind double stranded RNA elements.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Richard W. Baker, Janice M. Reimer, Peter J. Carman, Bengi Turegun, Tsutomu Arakawa, Roberto Dominguez, Andres E. Leschziner
Summary: The study reveals that the interaction between Sth1 and the nucleosome acidic patch enhances chromatin remodeling function, and large conformational changes are part of Sth1 regulation and RSC assembly.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thomas A. Johnson, Razvan V. Chereji, Diana A. Stavreva, Stephanie A. Morris, Gordon L. Hager, David J. Clark
NUCLEIC ACIDS RESEARCH
(2018)
Review
Biophysics
Razvan V. Chereji, David J. Clark
BIOPHYSICAL JOURNAL
(2018)
Article
Biochemistry & Molecular Biology
Ryota Ouda, Naoyuki Sarai, Vishal Nehru, Mira C. Patel, Maxime Debrosse, Mahesh Bachu, Razvan Chereji, Peter R. Eriksson, David J. Clark, Keiko Ozato
Article
Cell Biology
Yashpal Rawal, Razvan Chereji, Hongfang Qiu, Sudha Ananthakrishnan, Chhabi K. Govind, David J. Clark, Alan G. Hinnebusch
GENES & DEVELOPMENT
(2018)
Article
Biochemistry & Molecular Biology
Yashpal Rawal, Razvan V. Chereji, Vishalini Valabhoju, Hongfang Qiu, Josefina Ocampo, David J. Clark, Alan G. Hinnebusch
Article
Biochemistry & Molecular Biology
Gunjan D. Mehta, David A. Ball, Peter R. Eriksson, Razvan Chereji, David J. Clark, James G. McNally, Tatiana S. Karpova
Article
Multidisciplinary Sciences
Han-Wen Chang, Maria E. Valieva, Alfiya Safina, Razvan Chereji, Jianmin Wang, Olga Kulaeva, Alexandre Morozov, Mikhail P. Kirpichnikov, Alexey Feofanov, Katerina Gurova, Vasily M. Studitsky
Article
Biochemistry & Molecular Biology
Josefina Ocampo, Razvan Chereji, Peter R. Eriksson, David J. Clark
Article
Biochemistry & Molecular Biology
Hongfang Qiu, Emily Biernat, Chhabi K. Govind, Yashpal Rawal, Razvan Chereji, David J. Clark, Alan G. Hinnebusch
NUCLEIC ACIDS RESEARCH
(2020)
Article
Genetics & Heredity
Yashpal Rawal, Hongfang Qiu, Alan G. Hinnebusch
Summary: The nucleosome remodeling complexes SWI/SNF, RSC, and Ino80C cooperate in evicting or repositioning nucleosomes to produce nucleosome depleted regions (NDRs) at the promoters of many yeast genes induced by amino acid starvation. This cooperation involves enhancing the binding of transcriptional activator Gcn4 and recruitment of TATA-binding protein (TBP) during preinitiation complex (PIC) assembly. The three complexes are actively recruited by Gcn4 to bind at UAS elements and reduce nucleosome occupancies, indicating a positive regulatory loop. SWI/SNF also prevents excessive Gcn4 binding at UAS elements, indicating a dual mode of action that is modulated by the presence of RSC. In addition, RSC and SWI/SNF collaborate with Ino80C to enhance TBP recruitment and stimulate PIC assembly at Gcn4 target genes.
Article
Multidisciplinary Sciences
Rohit Prakash, Yashpal Rawal, Meghan R. Sullivan, McKenzie K. Grundy, Helene Bret, Michael J. Mihalevic, Hayley L. Rein, Jared M. Baird, Kristie Darrah, Fang Zhang, Raymond Wang, Tiffany A. Traina, Marc R. Radke, Scott H. Kaufmann, Elizabeth M. Swisher, Raphael Guerois, Mauro Modesti, Patrick Sung, Maria Jasin, Kara A. Bernstein
Summary: In this study, the authors investigated the mutations in RAD51C and found a cluster of variants that lead to HR deficiency and disruption of interactions with other RAD51 paralogs. Structural models were generated to explain the mechanisms of RAD51C interactions and ATP binding. Ovarian cancer patients with variants in this cluster showed longer survival, which may be relevant to the reversion potential of the variants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Medicine, Research & Experimental
Meghan R. Sullivan, Rohit Prakash, Yashpal Rawal, Weibin Wang, Patrick Sung, Marc R. Radke, Scott H. Kaufmann, Elizabeth M. Swisher, Kara A. Bernstein, Maria Jasin
Summary: The discovery of the RAD51C-T132P mutation in an ovarian cancer patient with unusually long progression-free survival suggests that this mutation affects HR function, drug sensitivity, and may be causative for tumor formation. Its position in a critical site may prevent secondary mutations that could restore gene function and lead to therapy resistance, underscoring the importance of understanding the relationship between mutation position and reversion potential of HR genes.
COLD SPRING HARBOR MOLECULAR CASE STUDIES
(2021)
Article
Biotechnology & Applied Microbiology
Razvan V. Chereji, Srinivas Ramachandran, Terri D. Bryson, Steven Henikoff