Article
Polymer Science
Krishnaroop Chaudhuri, Riddhiman Medhi, Zhenglin Zhang, Zhuoyun Cai, Christopher K. K. Ober, Jonathan T. T. Pham
Summary: In this study, the diffusion process of rhodamine B fluorescent dye in polystyrene-polydimethylsiloxane block copolymer coatings was visualized using confocal microscopy. The intensity of dye inside the coatings showed non-monotonic changes, attributed to concurrent dye molecule transport in different directions. An empirical fitting equation was used to compare contamination rates between copolymers, indicating that dye penetration is influenced by the chemical makeup and configuration of the BCPs. The research highlights the utility of confocal microscopy in visualizing the transport of fluorophores within coatings.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Guankui Wang, Markella Zannikou, Laren Lofchy, Yue Li, Hanmant Gaikwad, Irina Balyasnikova, Dmitri Simberg
Summary: The type of fluorescent label on liposomes significantly impacts their extravasation and accumulation in tumors. In animal experiments, indocarbocyanine lipids (ICLs) were found to spread and accumulate in tumor tissue more easily compared to fluorescent phospholipids (FPLs).
Article
Chemistry, Applied
Stephanie A. Kedzior, Sierra Cranmer-Smith, Natnael Behabtu, Kyle Kim, Christian Lenges, Steven L. Bryant, Milana Trifkovic
Summary: Exploiting the shape of Pickering stabilizers can enhance the stability of emulsions, with platelet-shaped particles found to reduce interfacial tension and result in the smallest droplet size. By manipulating particle shape, the microstructure and rheological properties of emulsions can be tuned to meet a wide range of application needs.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemical Research Methods
Subrata Kumar Padhan, Janmejaya Bag, Munmun Panda, Bijesh Kumar Biswal, Harekrushna Sahoo, Monalisa Mishra, Satya Narayan Sahu
Summary: In this study, a new fluorescent dye CTC was designed for selective binding to actin proteins, and its advantages were verified in Drosophila and cell lines. The dye can efficiently bind to actin without any secondary intermediate and exhibits excellent photostability. This is the first fluorescent dye that can bind to actin proteins without employing any secondary intermediate/actin-binding domain, opening up possibilities for imaging and tracking actin proteins in various in vivo and in vitro systems.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Analytical
Arnab Sarkar, Samaresh Hansda, Tiasha Dutta, Sourav Ghoshal, Sujay Mukhopadhyay, Pranab Sarkar, Sushil Kumar Mandal, Nimai Chandra Saha, Pabitra Chattopadhyay, Koushik Dhara
Summary: This study developed and synthesized two fluorescent probes, ER-NapNO2 and ER-NapAllyl, which can target the endoplasmic reticulum (ER) and selectively detect carbon monoxide (CO) in aqueous buffer medium. These probes showed selective detection behavior towards CO without the presence of any metal ions, and had high selectivity at low concentrations.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Sumayah Mohammed Asiri, Aviva Levina, Elizabeth J. New, Peter A. Lay
Summary: Imbalances in metal homeostasis have been found to be related to the progression and drug response of cancer cells. The interplay between copper homeostasis and the response to platinum-based chemotherapeutic agents has attracted significant attention. This study reveals differences in copper uptake and distribution in Pt-sensitive and resistant cells, which may be associated with different phenotypic advantages of the cells.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Physical
Maksim Gorbachevskii, Anna Stavitskaya, Andrei A. Novikov, Rawil F. Fakhrullin, Elvira Rozhina, Ekaterina A. Naumenko, Vladimir A. Vinokurov
Summary: Fluorescent gold nanoclusters (AuNCs) stabilized with 11-mercaptoundecanoic acid and supported on halloysite nanotubes modified with aminosilane showed good uptake by A549 human cells. At concentrations of 25-50 μg/mL, the material did not have a pronounced toxic effect but led to massive cell death via apoptosis at 100 μg/mL. This concentration-dependent toxicity mechanism allows for potential applications in bioimaging and cancer therapy.
APPLIED CLAY SCIENCE
(2021)
Article
Food Science & Technology
Seung-U Son, Ho Young Park, Hyung Joo Suh, Kwang-Soon Shin
Summary: The polysaccharides extracted from radish leaves showed antimetastatic effects by enhancing the cytotoxicity of natural killer cells and activating various immune factors, indicating potential as a therapeutic agent for inhibiting cancer metastasis.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Urology & Nephrology
Jose Marenco, Ana Calatrava, Juan Casanova, Francesco Claps, Juan Mascaros, Augusto Wong, Maria Barrios, Isabel Martin, Jose Rubio
Summary: FCM allows rapid diagnosis of prostate cancer with high concordance with traditional analysis. The technique shows promising results in terms of positive and negative predictive values, but further validation is needed.
EUROPEAN UROLOGY FOCUS
(2021)
Article
Chemistry, Multidisciplinary
Thomas M. D. Sheard, Tayla B. Shakespeare, Rajpinder S. Seehra, Michael E. Spencer, Kin M. Suen, Izzy Jayasinghe
Summary: Amine-reactive esters of aromatic fluorescent dyes are being used as imaging probes for staining cellular and tissue architectures. We characterized the staining patterns of 14 fluorescent dye ester species in human HeLa cells. By combining with expansion microscopy technique, the fluorescent esters can reveal nanoscale features of cells. The staining patterns vary depending on the properties of the dye and the timepoint of application in the protocol.
Article
Food Science & Technology
Seung-U Son, Han Wool Kim, Min Seung Park, Kwang-Soon Shin
Summary: The study aimed to determine the effects of polysaccharide isolated from fermented barley on tumor metastasis inhibition through immune related factors like the complement system, macrophages, NK cells, and CTL. It was found that the polysaccharide can inhibit tumor metastasis by activating immune responses and enhancing the cytotoxicity of CTL and NK cells against tumor cells.
Article
Food Science & Technology
Seung-U Son, Hee Won Lee, Kwang-Soon Shin
Summary: This study aimed to evaluate the anti-cancer effects and stimulatory effects on various immune related factors of polysaccharides isolated from Panax ginseng leaf. The results showed that these polysaccharides can enhance cytokine production and have anti-cancer effects.
Article
Chemistry, Applied
Nataliya Slovesnova, Artem S. Minin, Leonid T. Smolyuk, Olga S. Taniya, Anton N. Tsmokalyuk, Grigory A. Kim, Igor S. Kovalev, Varvara A. Pozdina, Dmitry S. Kopchuk, Alexey P. Krinochkin, Grigory Zyryanov, Alexander Yu Petrov, Valery N. Charushin
Summary: Two new water-soluble PAH-substituted naphtho[1,2-d][1,3]oxazole dyes were synthesized and studied for their intramolecular charge transfer behavior, aggregation, fluorescent staining, and photosensitization on living cancer cells. The relationship between their structure and photophysical properties was analyzed based on experimental and computation data. The results showed that these dyes exhibited diffuse distribution and accumulation in specific organelles of the cells, as well as demonstrated photodynamic activity.
Article
Agriculture, Multidisciplinary
Kyungae Jo, Singeun Kim, Kwang-Won Yu, Young Bae Chung, Woo Jung Kim, Hyung Joo Suh, Hoon Kim
Summary: Pretreatment of Dendrobium officinale with organic solvents may enhance the immunostimulatory activity of polysaccharides and affect the mannose/glucose ratio, with DOOS-CP showing stronger activity than DO-CP. Polysaccharides from puffed Dendrobium officinale exhibited significantly lower activity, with the most active polysaccharide containing over 95% neutral sugar and a mannose/glucose ratio of 3.0. Additionally, DOOS-CP was a refined fraction with a major peak representing a molecular weight of 250 kDa.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Medicine, General & Internal
Veronika Shavlokhova, Sameena Sandhu, Christa Flechtenmacher, Istvan Koveshazi, Florian Neumeier, Victor Padron-Laso, Zan Jonke, Babak Saravi, Michael Vollmer, Andreas Vollmer, Juergen Hoffmann, Michael Engel, Oliver Ristow, Christian Freudlsperger
Summary: This study investigated the potential of using convolutional neural networks (CNNs) for automated classification of oral squamous cell carcinoma via ex vivo FCM imaging. The model achieved promising results in classifying cancerous tissue, prompting further studies with larger sample sizes to introduce this technology into clinics.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)