Article
Medicine, Research & Experimental
Dingbang Xie, Yunteng Xu, Yimin Zhang, Wanping Cai, Xin Lan, Hui Yan
Summary: Postmenopausal osteoporosis (PMOP) is characterized by an imbalance between bone formation and bone resorption. In this study, it was found that icariin (ICA) could alleviate PMOP by inhibiting pyruvate dehydrogenase kinase 1 (PDK1) in osteoclasts, indicating its potential for clinical use.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cardiac & Cardiovascular Systems
Qinfeng Li, Chao Li, Abdallah Elnwasany, Gaurav Sharma, Yu A. An, Guangyu Zhang, Waleed M. Elhelaly, Jun Lin, Yingchao Gong, Guihao Chen, Meihui Wang, Shangang Zhao, Chongshan Dai, Charles D. Smart, Juan Liu, Xiang Luo, Yingfeng Deng, Lin Tan, Shuang-Jie Lv, Shawn M. Davidson, Jason W. Locasale, Philip L. Lorenzi, Craig R. Malloy, Thomas G. Gillette, Matthew G. Vander Heiden, Philipp E. Scherer, Luke I. Szweda, Guosheng Fu, Zhao V. Wang
Summary: PKM1 plays a crucial role in maintaining a homeostatic response in the heart under hemodynamic stress, protecting against cardiac dysfunction and fibrosis caused by pressure overload. Furthermore, PKM1 is essential for enhancing glycolytic flux, mitochondrial respiration, and ATP production under pressure overload at a mechanistic level.
Article
Virology
Wei Lu, Tingting Chen, Yimin Yao, Ping Chen
Summary: This study investigated the epidemiological characteristics of high-risk HPV in Zhejiang province, China, and found a significant association between HPV16 infection and cervical lesions. The presence of HPV16/18 was also linked to increased risk of developing high-grade cervical lesions and cancer progression in this population. These findings underscore the importance of HPV testing in early screening and prevention of cervical cancer.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tao Si, Xuejian Ning, Hongwei Zhao, Mingmin Zhang, Ping Huang, Zhengguo Hu, Liu Yang, Lizhu Lin
Summary: The study found that miR-9-5p promotes proliferation, invasion, and migration of HCC cells by regulating the expression of PDK4, enhancing mitochondrial activity and energy metabolism, while suppressing apoptosis, ultimately increasing tumorigenicity in mouse xenograft models. This suggests that miR-9-5p and PDK4 could be potential therapeutic targets for preventing recurrence and metastasis of HCC.
CANCER GENE THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Nives Skorja Milic, Klemen Dolinar, Katarina Mis, Urska Matkovic, Marusa Bizjak, Mojca Pavlin, Matej Podbregar, Sergej Pirkmajer
Summary: Inhibition of pyruvate dehydrogenase kinase (PDK) by Dichloroacetate (DCA) can reduce the abundance of PDK isoenzymes in a cell-type-specific manner via both transcriptional and post-transcriptional mechanisms. The differential response of PDK isoenzymes to DCA in cancer cells and muscle cells highlights the importance of understanding the pharmacological effects of DCA in different cell types.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Song Hee Lee, Bo Young Choi, A. Ra Kho, Dae Ki Hong, Beom Seok Kang, Min Kyu Park, Si Hyun Lee, Hui Chul Choi, Hong Ki Song, Sang Won Suh
Summary: The combined treatment of pyruvate and DCA shows protective effects against seizure-induced hippocampal neuronal cell death by increasing the proportion of pyruvate converted to ATP.
Article
Biochemistry & Molecular Biology
Yudi Ma, Xiaohui Lai, Zhongling Wen, Ziling Zhou, Minkai Yang, Qingqing Chen, Xuan Wang, Feng Mei, Liu Yang, Tongming Yin, Shucun Sun, Guihua Lu, Jinliang Qi, Hongyan Lin, Hongwei Han, Yonghua Yang
Summary: The Warburg effect is essential for tumor proliferation, but reversing it can lead to a novel anti-cancer strategy. Two key enzymes in tumor glucose metabolism pathway, PKM2 and PDK1, not only contribute to the Warburg effect, but also serve as druggable targets for CRC. A series of benzenesulfonyl shikonin derivatives were designed to simultaneously regulate PKM2 and PDK1. Among them, compound Z10 acted as a PKM2 activator and PDK1 inhibitor, significantly inhibiting glycolysis and remodeling tumor metabolism. In addition, Z10 inhibited CRC cell proliferation and migration, and induced apoptosis. Furthermore, Z10 showed in vivo anti-tumor activity with lower toxicity compared to shikonin. Our findings suggest that altering tumor energy metabolism through multi-target synergies is feasible, and Z10 could be a potential anti-CRC agent.
BIOORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yingchao Ma, Xishen Zhang, Xiao Han, Zhizhong Hao, Rong Ji, Jianhui Li, Wenna Guo, Yanting Zhang, Fangxia Guan, Shanshan Ma
Summary: Esophageal squamous cell carcinoma (ESCC) is a deadly malignancy with late-stage diagnosis and poor prognosis. In this study, PDK1 was found to play a role in ESCC by regulating metabolic reprogramming, and its inhibition using DCA showed promising antitumor effects. Additionally, the combination of DCA and cisplatin synergistically inhibited glycolysis and progression of ESCC cells through the Keap1/Nrf2 signaling pathway.
MOLECULAR CARCINOGENESIS
(2023)
Review
Medicine, Research & Experimental
Russel J. Reiter, Ramaswamy Sharma, Carmen Rodriguez, Vanesa Martin, Sergio Rosales-Corral, Debora Aparecida Pires de Campos Zuccari, Luiz Gustavo de Almeida Chuffa
Summary: This paper explores the relationship between endogenous melatonin and solid tumors, highlighting the potential of using melatonin to influence cancer cell metabolism. It suggests that solid tumors exhibit a healthier metabolic state at night, potentially reducing proliferation and invasiveness. The paper also proposes using melatonin in a time-sensitive treatment paradigm and combining it with traditional chemotherapy for improved outcomes in cancer treatment.
Article
Oncology
Esther P. Jane, Daniel R. Premkumar, Dhivyaa Rajasundaram, Swetha Thambireddy, Matthew C. Reslink, Sameer Agnihotri, Ian F. Pollack
Summary: This study demonstrates that targeting aurora kinases with tozasertib can effectively inhibit cell growth and migration in high-grade glioma cell lines, leading to cell death. However, sustained exposure to tozasertib can result in drug resistance, with upregulation of PDK genes playing a crucial role in the emergence of resistance.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Jennifer Margaret Roberts, Dorothy A. Machalek, Bethan C. Butler, Joanne Crescini, Suzanne M. Garland, Annabelle Farnsworth
Summary: In Australia's HPV-based cervical screening program, the risk of histological high-grade abnormality decreased with age in women with oncogenic HPV. This study followed HPV16/18 positive women aged 55 years and over for up to 3 years to determine the proportion with histological high-grade abnormality and its correlation with liquid-based cytology result and prior screening history. The results showed that age, screening history, and cytology result are important factors to consider in managing screen-detected abnormalities in HPV-based cervical screening programs.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Virology
Andreia Rosario, Ana Sousa, Joana Marinho-Dias, Rui Medeiros, Claudia Lobo, Luis Leca, Nuno Coimbra, Fernando Tavares, Ines Baldaque, Gabriela Martins, Paula Monteiro, Rui Henrique, Hugo Sousa
Summary: A study on cervical cancer screening in the Northern Region of Portugal revealed a 12.50% prevalence rate of high-risk human papillomavirus (Hr-HPV) infection. The most common Hr-HPV genotypes identified were HPV-68, HPV-31, HPV-51, HPV-16, and HPV-39. Hr-HPV infection was significantly associated with an increased risk of developing > HSIL. These findings provide important data for the future of vaccination and cervical cancer screening strategies.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Raquel Vaz, Josephine Wincent, Najla Elfissi, Kristina Rosengren Forsblad, Maria Pettersson, Karin Naess, Anna Wedell, Anna Wredenberg, Anna Lindstrand, Sofia Ygberg
Summary: This study reported a de novo heterozygous missense variant in the PDK1 gene in a girl with developmental delay and early onset severe epilepsy. Using a zebrafish model, the study showed the impact of this variant on energy metabolism and neuronal development, and the possible mechanism behind the clinical symptoms.
Article
Virology
Milena Camargo, Laura Vega, Marina Munoz, Ricardo Sanchez, Manuel Elkin Patarroyo, Juan David Ramirez, Manuel Alfonso Patarroyo
Summary: The study revealed a significant association between cervical microbiota and high-risk papillomavirus infections, with women with low viral loads exhibiting higher diversity and a greater likelihood of Lactobacillus dominance. This suggests that the abundance of Lactobacillus, diversity, and bacterial taxa enrichment play a crucial role in women with low viral loads.
Article
Medicine, General & Internal
Timothee Dub, Sophie Le Coeur, Nicole Ngo-Giang-Huong, Wanmanee Matanasarawut, Pornnapa Suriyachai, Kannikar Saisawat, Chaiwat Putiyanun, Sudanee Buranabanjasatean, Prattana Leenasirimakul, Samreung Randaeng, Tristan Delory
Summary: This study found that among women living with HIV, treatment of cervical lesions may be beneficial for clearing HR-HPV infections. After treatment, the prevalence of HR-HPV significantly decreased.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
