Article
Medicine, Research & Experimental
Wenbin Yang, Emily Jeong Cerier, Felix L. Nunez-Santana, Qiang Wu, Yuanqing Yan, Chitaru Kurihara, Xianpeng Liu, Anjana Yeldandi, Nigar Khurram, Diego Avella-Patino, Haiying Sun, G. R. Scott Budinger, Daniel Kreisel, Thalachallour Mohanakumar, Emilia Lecuona, Ankit Bharat
Summary: Preexisting lung-restricted autoantibodies (LRAs) are associated with a higher incidence of primary graft dysfunction (PGD) and can worsen graft dysfunction by causing damage to lung epithelial cells. LRA-mediated injury is distinct from ischemia-reperfusion injury and is mainly mediated by complement pathways and IL-1β-induced permeabilization of pulmonary vascular endothelium.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Review
Biochemistry & Molecular Biology
Guillaume Coutance, Eva Desire, Jean-Paul Duong Van Huyen
Summary: Despite advances in immunosuppression, allograft rejection remains a significant complication after heart transplantation. The current invasive diagnostic method has limitations, thus innovative strategies are needed to improve the accuracy of diagnosis.
Review
Medicine, Research & Experimental
Prathab Balaji Saravanan, Jagan Kalivarathan, Faizaan Khan, Rashi Shah, Marlon F. Levy, Mazhar A. Kanak
Summary: Exosomes secreted by cells can transfer signals and communicate with other cells, and the proteins, lipids, and genetic materials they contain can serve as potential biomarkers for assessing graft function and rejection status. Using exosomes to deliver therapeutic agonists/antagonists to the grafts can prevent rejection and reduce the side effects of immunosuppressive drugs. Exosomes play a critical role in the recognition and cross-presentation of donor organ-specific antigens during allograft rejection, and they have potential as biomarkers for monitoring graft function and damage, as well as therapeutic applications in mitigating allograft rejection.
Review
Biochemistry & Molecular Biology
Alessia Giarraputo, Ilaria Barison, Marny Fedrigo, Jacopo Burrello, Chiara Castellani, Francesco Tona, Tomaso Bottio, Gino Gerosa, Lucio Barile, Annalisa Angelini
Summary: Diagnosing cardiac allograft rejection post-heart transplantation is challenging, with tissue biopsies being the current gold standard. Non-invasive methods like liquid biopsy show promise for improving rejection diagnosis. Future advancements may involve molecular techniques and omics science to enhance monitoring accuracy for heart transplant patients.
Review
Immunology
Minoru Matsumoto, Hideyuki Yoshida, Koichi Tsuneyama, Takeshi Oya, Mitsuru Matsumoto
Summary: The thymus is crucial for establishing self-tolerance by educating developing T-cells. Recent single-cell studies have revealed heterogeneity in medullary thymic epithelial cells (mTECs) and provided insights into the mechanisms underlying tissue-restricted antigen (TRA) expression. This overview highlights the role of Aire in inducing mTEC heterogeneity to encompass TRAs.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Jan Novak, Tana Machackova, Jan Krejci, Julie Bienertova-Vasku, Ondrej Slaby
Summary: Congestive heart failure affects about 23 million people worldwide, and cardiac allograft transplantation is one of the last options for patients with terminal refractory heart failure. The prognosis of patients after heart transplantation is influenced by rejection and allograft vasculopathy, with monitoring requiring invasive and uncomfortable procedures. MiRNAs play a potential role in the pathophysiology of rejection and may be used in clinical practice for diagnosis and prediction of rejection occurrence.
Article
Cardiac & Cardiovascular Systems
Peter J. Kennel, Alexandre Yahi, Yoshifumi Naka, Donna M. Mancini, Charles C. Marboe, Klaas Max, Kemal Akat, Thomas Tuschl, Elena-Rodica M. Vasilescu, Emmanuel Zorn, Nicholas P. Tatonetti, Paul Christian Schulze
Summary: This study utilized next-generation small RNA sequencing to analyze circulating miRNAs as potential biomarkers for detecting and predicting allograft rejection after heart transplantation. It found three specific miRNAs with significantly increased levels in patients with cardiac rejection and identified potential predictors for acute cellular rejection (ACR) and antibody-mediated rejection (AMR), with hsa-miR-486-5p being strongly associated with acute rejection episodes. Monitoring allograft rejection using circulating miRNAs may offer an alternative to invasive endomyocardial biopsy.
Article
Cardiac & Cardiovascular Systems
Ranjithkumar Ravichandran, Yoshihiro Itabashi, Wei Liu, Sandhya Bansal, Mohammad Rahman, Christin Poulson, Timothy Fleming, Ross M. Bremner, Michael Smith, Thalachallour Mohanakumar
Summary: The study explored the mechanisms by which a decline in CCSP contributes to chronic lung allograft rejection in mouse models. It was found that induction of exosomes led to immune responses to donor MHC and lung self-antigens, resulting in CCSP decline, NK cell infiltration, and release of exosomes from NK cells. The results suggest a novel role for exosomes derived from NK cells in the pathogenesis of chronic lung allograft rejection.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
L. A. Erozenci, T. Pham, S. R. Piersma, N. F. J. Dits, G. W. Jenster, M. E. van Royen, R. J. A. Moorselaar, C. R. Jimenez, I. Bijnsdorp
Summary: Urinary extracellular vesicles (EVs) can be stored for up to 8 days at either 4 degrees C or room temperature with EDTA without significant decay in EV concentration or notable effects on the EV-proteome.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Brendan W. MacNabb, Sravya Tumuluru, Xiufen Chen, James Godfrey, Darshan N. Kasal, Jovian Yu, Marlieke L. M. Jongsma, Robbert M. Spaapen, Douglas E. Kline, Justin Kline
Summary: This study demonstrates the importance of MHC-I cross-dressing in regulating anti-tumor immunity. In addition to quantitatively enhancing tumor antigen presentation, it also enables dendritic cells to more accurately mirror the cancer cell peptidome.
Article
Multidisciplinary Sciences
Frederik Banch Clausen, Kristine Mathilde Clara Lund M. Jorgensen, Lasse Witt M. Wardil, Leif Kofoed M. Nielsen, Grethe Risum M. Krog
Summary: By using Droplet Digital PCR (ddPCR), we developed an assay setup for monitoring organ health. The results demonstrated that ddPCR has great sensitivity, high precision, and exceptional ability to quantify low levels of cfDNA. Further prospective studies are needed to determine the clinical utility of the proposed method.
Article
Immunology
Sarah B. See, Xue Yang, Carole Burger, Baptiste Lamarthee, Renaud Snanoudj, Ronzon Shihab, Demetra S. Tsapepas, Poulomi Roy, Stephanie Lariviere-Beaudoin, Katia Hamelin, Aleixandra Mendoza Rojas, Nicole M. van Besouw, Amanda Bartosic, Nikita Daniel, Vasilescu E. Rodica, Sumit Mohan, David Cohen, Lloyd Ratner, Carla C. Baan, Jonathan S. Bromberg, Heloise Cardinal, Dany Anglicheau, Yifei Sun, Emmanuel Zorn
Summary: This retrospective multicenter study found that pre- and posttransplant natural immunoglobulin G antibodies (Nabs) reactive to apoptotic cells were associated with poor graft outcomes and rejection after renal transplantation.
Article
Medicine, General & Internal
Wenyu Xiang, Shuai Han, Cuili Wang, Hongjun Chen, Lingling Shen, Tingting Zhu, Kai Wang, Wenjie Wei, Jing Qin, Nelli Shushakova, Song Rong, Hermann Haller, Hong Jiang, Jianghua Chen
Summary: In this study, RNA sequencing and bioinformatics methods were used to characterize the peripheral blood mononuclear cells of patients with acute renal allograft rejection. Differentially expressed genes and inflammatory pathways associated with rejection were identified, and a combination of genes was able to distinguish between rejection and non-rejection with high accuracy. This study provides a foundation for the discovery of biomarkers and a better understanding of acute renal allograft rejection.
FRONTIERS IN MEDICINE
(2022)
Article
Oncology
Jie Lin, Wansong Lin, Yannan Bai, Yanling Liao, Qiaoyan Lin, Lingfeng Chen, Yijuan Wu
Summary: In this study, exosomes were isolated directly from HCC tissues and analyzed for their potential as biomarkers for HCC. The expression profiles of exosomal miRNAs were studied, and hsa-miR-483-5p was identified as a potential biomarker for HCC diagnosis. In vitro experiments showed that ectopic expression of miR-483-5p promoted HCC cell proliferation by downregulating CDK15. This study highlights the importance of exosomal miRNAs in HCC and identifies a specific and sensitive biomarker for HCC diagnosis.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Oncology
Zoila A. Lopez-Bujanda, Aleksandar Obradovic, Thomas R. Nirschl, Laura Crowley, Rodney Macedo, Alexandros Papachristodoulou, Timothy O'Donnell, Uri Laserson, Jelani C. Zarif, Ran Reshef, Tiezheng Yuan, Mithil K. Soni, Emmanuel S. Antonarakis, Michael C. Haffner, H. Benjamin Larman, Michael M. Shen, Pawel Muranski, Charles G. Drake
Summary: This study identified a set of androgen-responsive genes as potential tumor-associated antigens for prostate cancer. Transglutaminase 4 (Tgm4) was found to be highly expressed in prostate tumors from luminal epithelial cells and had low expression in most non-prostate tissues. TGM4's immunogenicity was confirmed, suggesting its potential as an immunotherapy target for prostate cancer.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Review
Immunology
Ranjithkumar Ravichandran, Sandhya Bansal, Mohammad Rahman, Monal Sharma, Wei Liu, Ankit Bharat, Ramsey Hachem, Ashraf Omar, Michael A. Smith, T. Mohanakumar
Editorial Material
Immunology
Monal Sharma, Xunrong Luo
Article
Cardiac & Cardiovascular Systems
Muthukumar Gunasekaran, Sandhya Bansal, Ranjithkumar Ravichandran, Monal Sharma, Sudhir Perincheri, Francisco Rodriguez, Ramsey Hachem, Cynthia E. Fisher, Ajit P. Limaye, Ashraf Omar, Michael A. Smith, Ross M. Bremner, Thalachallour Mohanakumar
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2020)
Article
Cardiac & Cardiovascular Systems
Monal Sharma, Muthukumar Gunasekaran, Ranjithkumar Ravichandran, Cynthia E. Fisher, Ajit P. Limaye, Chengcheng Hu, John McDyer, Vaidehi Kaza, Ankit Bharat, Sofya Tokman, Ashraf Omar, Ashwini Arjuna, Rajat Walia, Ross M. Bremner, Michael A. Smith, Ramsey R. Hachem, Thalachallour Mohanakumar
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2020)
Article
Surgery
Monal Sharma, Ranjithkumar Ravichandran, Sudhir Perincheri, Lara Danziger-Isakov, Peter S. Heeger, Stuart C. Sweet, Thalachallour Mohanakumar
TRANSPLANT INTERNATIONAL
(2020)
Article
Immunology
Yoshihiro Itabashi, Ranjithkumar Ravichandran, Sandhya Bansal, Ankit Bharat, Ramsey Hachem, Ross Bremner, Michael Smith, T. Mohanakumar
Summary: The study found that lung transplant recipients with BOS had lower CCSP levels up to 9 months before BOS diagnosis, and those with SAgs antibodies 1 year post-transplant also developed DSA and had lower CCSP. Exosomes from BOS contained increased SAgs, NK cells markers, and cytotoxic molecules.
Article
Surgery
Camila Macedo, Lillian M. Tran, Alan F. Zahorchak, Helong Dai, Xinyan Gu, Ranjithkumar Ravichandran, Thalachallour Mohanakumar, Beth Elinoff, Adriana Zeevi, Mindi A. Styn, Abhinav Humar, Fadi G. Lakkis, Diana M. Metes, Angus W. Thomson
Summary: Infusing donor-derived regulatory dendritic cells (DCreg) into liver transplant patients can induce systemic changes in host antigen-presenting cells and T cells, potentially modulating anti-donor immune reactivity at the time of transplantation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Surgery
Ranjithkumar Ravichandran, Yoshihiro Itabashi, Timothy Fleming, Sandhya Bansal, Sara Bowen, Christin Poulson, Ankit Bharat, Ross Bremner, Michael Smith, Thalachallour Mohanakumar
Summary: Low-dose IL-2 treatment delays chronic cardiac allograft rejection and induces the generation of regulatory cells and circulating exosomes with immunoregulatory molecules.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Meeting Abstract
Surgery
R. Ravichandran, Y. Itabashi, W. Liu, M. Rahman, S. Bansal, R. Bremner, M. Smith, T. Mohanakumar
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Meeting Abstract
Surgery
R. Ravichandran, Y. Itabashi, R. Heilman, A. Jaramillo, D. Ramon, M. Buras, B. Kaplan, T. Mohanakumar, S. Sukumaran Nair
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Meeting Abstract
Surgery
Y. Itabashi, R. Ravichandran, S. Bansal, T. Fleming, T. Mohanakumar
AMERICAN JOURNAL OF TRANSPLANTATION
(2020)
Meeting Abstract
Cardiac & Cardiovascular Systems
R. Ravichandran, Y. Itabashi, W. Liu, T. Mohanakumar
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2020)