Article
Plant Sciences
Elizabeth Alejandra Motolinia-Alcantara, Adrian Marcelo Franco-Vasquez, Antonio Nieto-Camacho, Roberto Arreguin-Espinosa, Mario Rodriguez-Monroy, Francisco Cruz-Sosa, Angelica Roman-Guerrero
Summary: It was found that in vitro culture of Ageratina pichichensis can produce phenolic compounds with antioxidant activity. Callus culture showed the highest phenolic content, followed by cell suspension culture. Compared with wild plants, in vitro cultures had lower levels of phenolic compounds, but some compounds not found in wild plants were detected in the in vitro cultures.
Article
Biodiversity Conservation
Yuan-yuan Meng, Shi-jie Song, Sven Landrein
Summary: The study provides the first protocol for in vitro organogenesis and plant regeneration for Passiflora xishuangbannaensis, aiming at conservation and reintroduction efforts. Genetic stability of regenerated cells and analysis of genetic variation between the plantlets and the original plant were conducted. The response to plant growth regulators in this Asian species was found to be more specific, potentially due to differences in morphology and physiology between South American and Asian Passiflora species.
GLOBAL ECOLOGY AND CONSERVATION
(2021)
Article
Biochemistry & Molecular Biology
Zeynep Ergun
Summary: This study examined the effects of different plant growth regulators on the fatty acid composition of Ricinus communis L. samples, showing that the type and concentration of plant growth regulators can significantly impact the fatty acid profiles, with IAA having the largest effect.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Horticulture
Jingting Wang, Xinhui Xia, Gaihua Qin, Jingwen Tang, Jun Wang, Wenhao Zhu, Ming Qian, Jiyu Li, Guangrong Cui, Yuchen Yang, Jingjing Qian
Summary: In this study, an effective system for inducing high-vigor pomegranate somatic embryos from stem explants was developed. Through optimization, the best induction conditions were found to be a Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-benzylaminopurine (6-BA) and 1.0 mg/L 1-naphthaleneacetic acid (NAA), achieving an induction rate of approximately 72%. The optimal conditions for the induction of embryogenic calli and somatic embryos were MS medium containing 0.5 mg/L 6-BA and 1.0 mg/L NAA, with induction rates of approximately 74% and 79%, respectively. The results demonstrate that our system provides a solution for mass somatic embryo induction and plant regeneration of pomegranate.
Article
Green & Sustainable Science & Technology
Tamara S. Al-Qudah, Rida A. Shibli, Ahmad Zatimeh, Reham W. Tahtamouni, Firas Al-Zyoud
Summary: This research aims to establish a comprehensive and sustainable approach for the in vitro propagation and conservation of the important wild medicinal plant Salvia dominica L. in Jordan and neighboring countries. Through in vitro culture and cryopreservation experiments, a successful protocol was developed for the propagation and conservation of S. dominica calli.
Article
Horticulture
Roya Razavizadeh, Fatemeh Adabavazeh, Marzieh Rezaee Chermahini, Setsuko Komatsu
Summary: Higher production of secondary metabolites is induced by salinity in Carum copticum. Callus formation and plant regeneration were analyzed, and salinity enhanced the content of reducing sugars, proline, antioxidant enzymes, phenolics, anthocyanin, and essential oils in C. copticum.
Article
Plant Sciences
Arda Acemi, Sevgi Turker-Kaya
Summary: The study compared the effects of chitosan oligomers and polymeric chitosan with various plant growth regulators on the root system of Serapias vomeracea, indicating that chitosan may exhibit cytokinin-like or jasmonic acid-like activity in the root system. The degree of polymerization of chitosan may also influence its effects on root system development in S. vomeracea.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
Review
Plant Sciences
Luis Alberto Bravo-Vazquez, Paola Isabel Angulo-Bejarano, Anindya Bandyopadhyay, Ashutosh Sharma, Sujay Paul
Summary: Plant regulatory noncoding RNAs (ncRNAs) play a key role in modulating gene expression during callus induction, and further research may lead to innovative plant tissue culture protocols.
PLANT CELL REPORTS
(2023)
Article
Forestry
Ge Guo, Byoung Ryong Jeong
Summary: The Korean fir is a unique tree species in Korea, but many have died due to climate change. This study investigated callus induction using different explants, media, and plant growth regulators. It was found that stem segments were more suitable for callus induction, with the Murashige and Skoog medium being the most effective and PGR combinations containing 2,4-D showing positive impact on callus induction and proliferation.
Review
Plant Sciences
Valeria Cavallaro, Alessandra Pellegrino, Rosario Muleo, Ivano Forgione
Summary: Plant tissue cultures rely on artificial light sources for growth and metabolism. By controlling light quality, intensity, and duration, desired plant characteristics can be achieved and energy can be saved. However, the effects of light on in vitro propagation are conflicting, due to various factors and the lack of a standardized protocol.
Article
Biochemistry & Molecular Biology
Mat Yunus Najhah, Hawa Z. E. Jaafar, Jaafar Juju Nakasha, Mansor Hakiman
Summary: The study found that in vitro-cultured callus of L. pumila var. alata displayed significantly higher antioxidant activities and total phenolic and flavonoid content compared to leaf extracts from in vitro plantlets and wild plants. This suggests that callus culture could potentially be utilized for antioxidant and polyphenolic production, meeting pharmaceutical and nutraceutical needs while also conserving the wild L. pumila var. alata.
Review
Plant Sciences
Kai Hao, Yun Wang, Zhanpin Zhu, Yu Wu, Ruibing Chen, Lei Zhang
Summary: This review summarizes the recent progress on the contributions of the miR160-ARF module to important traits of plants and the role of miR160-centered gene regulatory network in coordinating growth with endogenous and environmental factors. Manipulation of miR160-guided gene regulation may provide a new method to engineer plants with improved adaptability and yield.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Chitralekha Shyam, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Ravindra Singh Solanki, Swapnil Sapre, Ashok Ahuja, Sharad Tiwari
Summary: Brassica juncea is a principal oilseed crop of India and Madhya Pradesh, with somaclonal variations explored as a potential source of additional variability for manipulating fatty acids, especially low erucic acid content. Putative somaclones derived from tissue cultures showed varying levels of erucic acid compared to parental lines, providing a potential source of variation for developing future mustard crops with low erucic acid content.
Article
Plant Sciences
Wenhui Song, Yaohong Song, Xueting Liu, Xiaoju Zhang, Rujie Xin, Siyang Duan, Shixin Guan, Xiaomei Sun
Summary: In this study, an efficient in vitro system for callus induction and regeneration in herbaceous peony (Paeonia lactiflora Pall.) was developed by improving culture conditions and using plant growth regulators in the culture media. This system provides a foundation for subsequent genetic engineering research and enables rapid propagation and large-scale production of P. lactiflora.
Article
Plant Sciences
Nishi Mishra, Manoj Kumar Tripathi, Sushma Tiwari, Niraj Tripathi, Swapnil Sapre, Ashok Ahuja, Sharad Tiwari
Summary: This experiment explored somaclonal variation as a method to increase drought tolerance in soybean. By treating calli/cell clumps/embryoids, drought-tolerant plantlets were successfully regenerated, confirming the presence of variability through random decamer primers.
Article
Forestry
Mohsen Hesami, Mohammad Hosein Daneshvar, Mohsen Yoosefzadeh-Najafabadi
JOURNAL OF FORESTRY RESEARCH
(2019)
Article
Forestry
Mohsen Hesami, Masoud Tohidfar, Milad Alizadeh, Mohammad Hosein Daneshvar
JOURNAL OF FORESTRY RESEARCH
(2020)
Article
Plant Sciences
Mohsen Hesami, Roohangiz Naderi, Masoud Tohidfar, Mohsen Yoosefzadeh-Najafabadi
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Biochemical Research Methods
Mohsen Hesami, Roohangiz Naderi, Masoud Tohidfar, Mohsen Yoosefzadeh-Najafabadi
Article
Chemistry, Multidisciplinary
Mohsen Hesami, Jorge A. Condori-Apfata, Maria Valderrama Valencia, Mohsen Mohammadi
APPLIED SCIENCES-BASEL
(2020)
Article
Biochemistry & Molecular Biology
Mohsen Hesami, Mohsen Yoosefzadeh Najafabadi, Kristian Adamek, Davoud Torkamaneh, Andrew Maxwell Phineas Jones
Summary: The study evaluates the predictive ability of machine learning algorithms and ensemble-bagging strategy to predict sgRNA off-target activity in cannabis, with the random forest (RF) algorithm showing the best performance. Using RF algorithm as a meta-classifier for E-B method enhances prediction accuracy and F-measure. The E-B algorithm demonstrates the highest AUC-PRC and AUC-ROC values, indicating its success as a common ensemble strategy.
Review
Biochemistry & Molecular Biology
Mohsen Hesami, Austin Baiton, Milad Alizadeh, Marco Pepe, Davoud Torkamaneh, Andrew Maxwell Phineas Jones
Summary: Cannabis sativa has been historically used for therapeutic and industrial purposes, but new biotechnological tools are needed to introduce new genotypes and enhance secondary metabolite production. Various methods like micropropagation and gene transformation have been studied, but challenges such as low transgenic plant regeneration and efficiency of secondary metabolite production still exist. Promising techniques like CRISPR and hairy root culture have potential to improve gene transformation, plant regeneration, and secondary metabolite production in cannabis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Marco Pepe, Mohsen Hesami, Andrew Maxwell Phineas Jones
Summary: In vitro seed germination of cannabis faces challenges in uniformity and germination time due to the disinfection procedure. Artificial intelligence models, like the generalized regression neural network (GRNN), were used to optimize different disinfectants and immersion times for contamination reduction. The optimized protocol resulted in 0% contamination and 100% seed germination when combined with seed scarification.
Article
Plant Sciences
Mohsen Hesami, Marco Pepe, Austin Baiton, Seyed Alireza Salami, Andrew Maxwell Phineas Jones
Summary: Cannabis has potential as an ornamental plant, but legal barriers have hindered its development. Research shows that cannabis has high value as a potted-plant or in landscaping. However, the legalization and breeding for ornamental traits will depend on its legal status and require time. The use of cannabis chemotype ? with almost no phytocannabinoids and psychoactive properties for breeding offers opportunities for creating ornamental cannabis.
Review
Genetics & Heredity
Mohsen Yoosefzadeh Najafabadi, Mohsen Hesami, Milad Eskandari
Summary: In the face of a growing global population, plant breeding is being used as a sustainable tool for increasing food security. With the use of high-throughput omics technologies, data on the genetic architecture of plants can be exploited for improving crop characteristics. The use of big data coupled with machine learning algorithms has the potential to revolutionize plant breeding and increase food security.
Review
Plant Sciences
Mohsen Yoosefzadeh Najafabadi, Mohsen Hesami, Istvan Rajcan
Summary: Non-Mendelian heredity plays a crucial role in plant breeding for developing new varieties. It involves various factors like cytoplasmic inheritance, methylation, epigenetics, hybrid vigor, and LOH that can influence inheritance patterns besides genetic architecture. Understanding these mechanisms, their prevalence, and implications for plant breeding is essential to accelerate crop improvement.
Article
Biology
Mohsen Hesami, Kristian Adamek, Marco Pepe, Andrew Maxwell Phineas Jones
Summary: This study evaluates the impact of explant source within the cannabis plant on growth and development in vitro. It suggests that basal-derived explants have better performance and less phenotypic variation compared to middle and apical-derived explants. Therefore, it is recommended to use basal-derived tissues as the starting material for cannabis micropropagation.
Article
Biochemistry & Molecular Biology
Mohsen Hesami, Marco Pepe, Maxime de Ronne, Mohsen Yoosefzadeh-Najafabadi, Kristian Adamek, Davoud Torkamaneh, Andrew Maxwell Phineas Jones
Summary: In this study, the differential gene expression profiles of various cannabis calli were examined to enhance our understanding of callus development in cannabis and improve plant regeneration and biotechnological applications. The results revealed the crucial role of plant growth regulator signaling, transcription factors, and epigenetic regulation in callus development. These findings provide comprehensive insights into gene expression dynamics and molecular mechanisms underlying the development of diverse cannabis calli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Mohsen Hesami, Marco Pepe, Andrew Maxwell Phineas Jones
Summary: This study extensively characterizes the morphological changes of Cannabis sativa L. cv. White Widow throughout its life cycle, with a focus on leaf morphology, plant structure, flower development, and trichome features. The developmental responses to photoperiodic variations were also investigated. The findings provide valuable insights for industrial and research applications.
Article
Horticulture
Marziyeh Jafari, Ali Reza Shahsavar, Majid Talebi, Mohsen Hesami
Summary: Melatonin treatment can protect lime plants from damage caused by drought stress by reducing electrolyte leakage and oxidative stress, increasing antioxidant enzyme activity and proline content.