Article
Genetics & Heredity
Mohammad Foad Abazari, Shohreh Zare Karizi, Nazanin Hajati-Birgani, Sara Norouzi, Zahraalsadat Khazeni, Javad Hashemi, Lida Shafaghi, Fatemeh Soleimanifar, Reyhaneh Nassiri Mansour, Seyed Ehsan Enderami
Summary: The use of PHBV nanofibers as scaffolds promoted the differentiation of induced pluripotent stem cells (iPSCs) into insulin-producing cells (IPC) for diabetic treatment. iPSCs cultured on PHBV nanofibers showed significantly higher survival rates, increased insulin and C-peptide secretion levels, and upregulation of genes and insulin protein expression compared to cells cultured on traditional tissue culture polystyrene (TCPS).
Article
Oncology
Javad Hashemi, Abdol-Mohammad Kajbafzadeh, Fariba Ghorbani, Masoud Soleimani, Ehsan Arefian, Reza Khorramirouz, Seyed Ehsan Enderami, Parvin Pasalar
Summary: This study aimed to engineer pancreatic tissue by decellularizing intact rat pancreases and reseeding them with human-induced pluripotent stem cells using different growth factors. The differentiated cells in Exp2 3D culture exhibited significant upregulation of pancreatic endocrine markers and glucose transporter, with the highest insulin and C-peptide concentrations. The presence of decellularized pancreas as a scaffold for hiPSCs and growth factors shows potential for applications.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Endocrinology & Metabolism
Diego Balboa, Diepiriye G. Iworima, Timothy J. Kieffer
Summary: The review discusses the recent progress in generating and characterizing functional stem cell-derived beta cells, as well as the possibilities and challenges in diabetes disease modeling using stem cells.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Erin M. MacFarlane, Jennifer E. Bruin
Summary: The prevalence of diabetes is increasing globally, with a reported association between diabetes and environmental pollutant exposure. Identifying chemicals that disrupt metabolism, particularly those affecting pancreatic endocrine cells, is a high priority. Human pluripotent stem cells show promise as a tool for in vitro toxicity testing and screening for chemicals influencing endocrine cell formation.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Materials Science, Biomaterials
Divya Sridharan, Arunkumar Palaniappan, Britani N. Blackstone, Julie A. Dougherty, Naresh Kumar, Polani B. Seshagiri, Nazish Sayed, Heather M. Powell, Mahmood Khan
Summary: The study demonstrates that coaxial PCL-gelatin nanofibrous scaffolds can be used as a 3D culture platform for efficient differentiation of hiPSCs to functional cardiomyocytes. While there were no significant differences in the morphology and immunofluorescence staining of cardiomyocytes between 2D and 3D cultures, gene expression data showed increased expression of cardiac progenitor genes in 3D cultures and cardiomyocytes markers in 2D cultures.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Review
Immunology
Ayman F. Refaie, Batoul L. Elbassiouny, Malgorzata Kloc, Omaima M. Sabek, Sherry M. Khater, Amani M. Ismail, Rania H. Mohamed, Mohamed A. Ghoneim
Summary: MSC-based therapy for T1DM shows promise, but there are conflicting views on their immunological profile. Research focuses on understanding the mechanisms of their immunomodulatory functions and exploring ways to enhance their effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maciej Sulkowski, Marta Kot, Bogna Badyra, Anna Paluszkiewicz, Przemyslaw M. Plonka, Michal Sarna, Dominika Michalczyk-Wetula, Fabio A. Zucca, Luigi Zecca, Marcin Majka
Summary: The study introduces an innovative method for inducing pluripotent stem cells differentiation into melanin-producing cells, generating highly pigmented cells in quantity and quality incomparably higher than previous methods. These melanin-producing cells exhibit characteristics similar to melanocytes and serve as a potential unlimited source for modeling human skin diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Ana Margarida Carvalho, Rute Nunes, Bruno Sarmento
Summary: Type 1 diabetes mellitus affects millions of people worldwide and is rapidly increasing. Current treatments rely on exogenous insulin administration, but the control of glycemia is not precise. Pluripotent stem cells offer a potential solution as they can be differentiated into insulin-producing cells and restore glycemic control. Overcoming the challenges of stem cell therapy would greatly improve the quality of life for individuals with type 1 diabetes mellitus.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Cell & Tissue Engineering
Song Lee, Jae Hyun Joo, Ju Yun Oh, Eun Ha Seo, Yang Hee Kim, Eunsung Jun, In Kyong Shim, Song Cheol Kim
Summary: Research compared combinations of small molecules for inducing cell differentiation, finding that specific small molecules could promote the generation of insulin-producing cells that secreted insulin in response to glucose stimulation. This simplified differentiation method provides a potential IPC preparation strategy for clinical trials.
STEM CELLS INTERNATIONAL
(2021)
Article
Engineering, Biomedical
Hui Huang, Soujanya S. Karanth, Ya Guan, Sebastian Freeman, Ryan Soron, David S. Godovich, Jianjun Guan, Kaiming Ye, Sha Jin
Summary: This study utilizes oxygen-releasing biomaterials and an oxygenated scaffold culture platform to enhance oxygen supply during 3D iPSC pancreatic differentiation. The oxygenation does not affect the scaffold's mechanical properties, but improves oxygen tension within the scaffolds. The unique 3D differentiation system enables the generation of islet organoids with enhanced expression of islet signature genes and proteins, as well as improved sensitivity to glucose for insulin secretion.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Endocrinology & Metabolism
Juan Ignacio Burgos, Ludovic Vallier, Santiago A. Rodriguez-Segui
Summary: Modeling monogenic diabetes using human pluripotent stem cells allows for detailed analysis of disease-associated gene regulatory mechanisms and has revealed new insights into the disease. This approach has the potential to improve understanding of monogenic diabetes pathogenesis and therapeutic strategies.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Medicine, Research & Experimental
Yamini Chandramohan, Kavya Jeganathan, Sanjana Sivanesan, Pavani Koka, Trikkur Madom Seetaraman Amritha, Selvaraj Vimalraj, Anuradha Dhanasekaran
Summary: This study successfully isolated mesenchymal stem cells (MSCs) from human Ovarian Follicular Fluid (OFF) for the first time, and characterized them through various methods. The growth of MSCs with the conditioned medium obtained from a biocomposite scaffold promoted osteoblast differentiation, indicating good culture conditions and applicability.
Review
Cell Biology
Kevin Verhoeff, Sarah J. Henschke, Braulio A. Marfil-Garza, Nidheesh Dadheech, Andrew Mark James Shapiro
Summary: Despite advances in diabetes treatment, challenges remain in replicating physiological euglycemia and addressing organ supply limitations. Islet cell transplantation and stem cell therapy show promise for the future but face barriers to widespread use that must be addressed.
Article
Cell Biology
Pengxiu Dai, Jiakai Li, Yijing Chen, Luwen Zhang, Xinke Zhang, Jinglu Wang, Guixiang Qi, Yihua Zhang
Summary: This study identified important genes and signaling pathways during the transdifferentiation of ADMSCs into IPCs through transcriptome sequencing analysis, further demonstrating the significant effects of Foxa1, Pbx1, and Rfx3 in the transdifferentiation process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Polymer Science
Raul Rosales-Ibanez, Alejandro Emmanuel Viera-Ruiz, Juan Valerio Cauich-Rodriguez, Hugo Joel Carrillo-Escalante, Arely Gonzalez-Gonzalez, Jesus Jiovanni Rodriguez-Martinez, Fernando Hernandez-Sanchez
Summary: This work focuses on manufacturing a scaffold for tissue engineering of the bone system. The scaffold is shaped like a rabbit tibia and is obtained using a 3D-printer. The scaffold is then covered with a layer of synthesized polycaprolactone to improve cell adhesion. The scaffold is characterized using various methods to evaluate its biocompatibility and osteogenic differentiation capability.
Article
Pharmacology & Pharmacy
Mohammad Foad Abazari, Sepehr Torabinejad, Shohreh Zare Karizi, Hadi Samadian, Sahar Jalili-ghelichi, Mehdi Norouzi, Hassan Askari, Vahdat Poortahmasebi, Seyedeh Elnaz Enderami, Fatemeh Soleimanifar
Summary: This study demonstrates the high osteo-supportive capacity of a modified nanofibrous scaffold using human placental extract. It provides a potential candidate for bone tissue engineering.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Mohammad Foad Abazari, Sepehr Torabinejad, Shohreh Zare Karizi, Seyedeh Elnaz Enderami, Hadi Samadian, Nazanin Hajati-Birgani, Sara Norouzi, Fatemeh Nejati, Assil Al Bahash, Vahid Mansouri
Summary: This study aimed to investigate the impact of combining hydrophilic polymers with hydrophobic scaffolds on the osteogenic differentiation of induced pluripotent stem cells. The results showed that iPSCs grown on PCL/PVA/CP had the highest osteoinductive potential compared to other groups.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Vahdat Poortahmasebi, Ahmad Nejati, Mohammad Foad Abazari, Mohsen Nasiri Toosi, Azam Ghaziasadi, Nader Mohammadzadeh, Ahmad Tavakoli, Azam Khamseh, Navid Momenifar, Omid Gholizadeh, Mehdi Norouzi, Seyed Mohammad Jazayeri
Summary: This study used RNA-sequencing to analyze the gene expression differences in PBMCs of HCC patients and identified DEGs related to immune response. Additionally, 7 genes with important diagnostic value for HBV-HCC were screened. These results provide new insights into the pathogenesis and clinical diagnosis of HCC.
CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Engineering, Biomedical
Reyhaneh Nassiri Mansour, Ayoob Karimizade, Seyed Ehsan Enderami, Mozhgan Abasi, Fereshteh Talebpour Amiri, Abdolreza Jafarirad, Amir Mellati
Summary: This study investigated the effects of animal age, decellularization protocols, and sterilization methods on xenogeneic acellular dermal matrices (ADMs) for skin regeneration. The results show that fetal source and EO-sterilized samples performed better in terms of morphology, mechanical properties, and wound treatment. There was no added value in using enzymatic treatment during the decellularization process. The findings suggest that the selection of animal age, decellularization, and sterilization methods should be based on clinical requirements.
Article
Engineering, Biomedical
Seyedeh Fatemeh Ahmadi, Reyhaneh Nassiri Mansour, Hadi Hassannia, Seyed Ehsan Enderami, Saeid Abediankenari, Zahra Hosseini-khah
Summary: This study evaluated the differentiation of iPSCs into IPCs on a 3D scaffold and compared it with a 2D cultured group. The results showed that the differentiated cells on the 3D scaffold had higher expression of pancreatic specific-markers and released more insulin and C-peptide compared to the 2D group.
Article
Biochemistry & Molecular Biology
Fatemeh Hosseinpour-Soleimani, Gholamreza Khamisipour, Zahra Derakhshan, Bahram Ahmadi
Summary: This study aimed to determine the expression levels of miRNAs in patients with acute lymphoblastic leukemia (ALL) and evaluate their value as biomarkers or therapeutic targets. The results showed that miR-217 was significantly decreased, while miR-22, miR-122, and miR-367 were increased. ROC analysis demonstrated that miR-217 and miR-367 could differentiate ALL patients from healthy individuals.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Pharmacology & Pharmacy
Serena Sagliocchi, Melania Murolo, Annunziata Gaetana Cicatiello, Caterina Miro, Annarita Nappi, Emery Di Cicco, Sepehr Torabinejad, Evelina La Civita, Veronica Romano, Daniela Terracciano, Mariano Stornaiuolo, Monica Dentice
Summary: The iodothyronine deiodinases are a group of enzymes that regulate thyroid hormone metabolism and affect various physiological processes. Deregulation of these enzymes is associated with several human pathologies. This study identifies Cefuroxime as a novel inhibitor of D2 activity, potentially offering a new treatment option for hyperthyroid pathologies.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Annarita Nappi, Caterina Miro, Antonio Pezone, Alfonso Tramontano, Emery Di Cicco, Serena Sagliocchi, Annunziata Gaetana Cicatiello, Melania Murolo, Sepehr Torabinejad, Elena Abbotto, Giuseppina Caiazzo, Maddalena Raia, Mariano Stornaiuolo, Dario Antonini, Gabriella Fabbrocini, Domenico Salvatore, Vittorio Enrico Avvedimento, Monica Dentice
Summary: In this study, the authors investigate the role of thyroid hormones in tumor progression and identify the thyroid hormone activating enzyme D2 as a p53 target gene. They find that p53, as a cell stress sensor and tumor suppressor, suppresses D2 expression, leading to decreased availability of intracellular thyroid hormones. Conversely, loss of p53 enhances D2/TH signaling, promoting the fitness of tumor cells through modulation of genes involved in DNA damage and repair and redox signaling. In vivo deletion of D2 significantly reduces cancer progression, suggesting that targeting thyroid hormones could be a general approach to reduce invasiveness in p53-mutated neoplasms.
NATURE COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Reyhaneh Nassiri Mansour, Elham Hasanzadeh, Mozhgan Abasi, Mazaher Gholipourmalekabadi, Amir Mellati, Seyed Ehsan Enderami
Summary: Combining fetal bovine acellular dermal matrix (FADM) with human Wharton's jelly mesenchymal stem cells (hWJ-MSCs) can promote the healing of full-thickness skin wounds. The FADM-WJMSC group showed a higher wound closure rate and fewer inflammatory cells compared to the control and FADM groups. These findings suggest the potential application value of combining FADM with hWJ-MSCs in the treatment of skin wounds.
Article
Biochemistry & Molecular Biology
Caterina Miro, Annarita Nappi, Serena Sagliocchi, Emery Di Cicco, Melania Murolo, Sepehr Torabinejad, Lucia Acampora, Arianna Pastore, Paolo Luciano, Evelina La Civita, Daniela Terracciano, Mariano Stornaiuolo, Monica Dentice, Annunziata Gaetana Cicatiello
Summary: Thyroid hormones play a role in regulating muscle formation and energy utilization by modulating carbohydrates, lipids, and proteins. They can change the lipid composition and fatty acid ratio in skeletal muscle, with increased proportion of unsaturated fatty acids improving insulin sensitivity and endurance exercise.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Sepehr Torabinejad, Caterina Miro, Biagio Barone, Ciro Imbimbo, Felice Crocetto, Monica Dentice
Summary: There is increasing evidence that thyroid hormones and androgen receptors work together to regulate gonadal differentiation and reproductive function. These hormones have interactions at the promoter region and increase expression of androgen receptors. The crosstalk between thyroid hormones and androgen receptors plays a role in the regulation of enzymes involved in androgen biosynthesis, indicating a mutual interaction. This interaction supports the relationship between hyperthyroidism and the high incidence of prostate cancer (PCa).
EUROPEAN THYROID JOURNAL
(2023)
Article
Cell Biology
Mostafa Ebrahimdoost, Mohsen Mohammadi, Narges Obeidi, Seyed Amin Mohammadi, Gholamreza Khamisipour
Summary: The anticancer activity of AMP-WF3 peptide from Poecilia Mexicana fish was evaluated against leukemic cell line Jurkat, and its cytotoxicity compared with normal cells. AMP-WF3 exhibited higher cytotoxicity against Jurkat cells with minimal effects on normal cells. The apoptotic activity of AMP-WF3 on Jurkat cells was significantly higher than that on HDF and PBMCs. The expression of P21, P53, and BCL-2 genes showed different changes in Jurkat cells, PBMCs, and HDF cells.
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.
