Article
Microbiology
Bing Sun, Huaizhi Luo, Huan Jiang, Zhennan Wang, Aiqun Jia
Summary: Esculetin exhibited inhibitory effects on biofilm formation and quorum sensing of Aeromonas hydrophila, reducing the production of protease and hemolysin, attenuating swarming motility, and showing downregulation of genes related to QS and biofilm formation while upregulating gene litR. Phenotypic results were consistent with gene expression levels, suggesting potential as a QS inhibitor for A. hydrophila.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Liangting Shao, Shanshan Chen, Zhenzhen Ning, Xinglian Xu, Huhu Wang
Summary: In this study, a hemagglutinin protease (Hap) belonging to the M4 family metallopeptidase was identified from Aeromonas salmonicida 29 isolate, which is conserved in various dominant spoilage bacteria. Hap demonstrated high spoilage ability on meat, increasing TVB-N, pH values, and the production of volatile organic compounds (VOCs), confirming its determinant role for meat spoilage. These findings will enrich the spoilage theory and provide new insights into the control of food quality and safety.
Article
Fisheries
Shengping Li, Shun Zhou, Qiuhong Yang, Yongtao Liu, Yibin Yang, Ning Xu, Xiaohui Ai, Jing Dong
Summary: A study found that cinnamaldehyde, extracted from a natural plant, has inhibitory effects on Aeromonas hydrophila infection. Cinnamaldehyde disrupts the bacterial quorum sensing system, reducing the pathogenic behaviors of A. hydrophila without affecting bacterial growth. It was also observed that cinnamaldehyde downregulates the expression of related genes and can effectively decrease host cell injury and increase the survival rate in A. hydrophila-infected hosts. These results suggest that cinnamaldehyde could be a potential candidate as a quorum sensing inhibitor against A. hydrophila infection.
Article
Biotechnology & Applied Microbiology
Shengping Li, Qiuhong Yang, Bo Cheng, Yongtao Liu, Shun Zhou, Xiaohui Ai, Jing Dong
Summary: Neem oil was found to inhibit the quorum-sensing of A. hydrophila and reduce the production of various virulence factors at sub-inhibitory concentrations. Real-time PCR confirmed that neem oil could regulate the transcription of relevant genes, and it exhibited significant protection in cell and animal models.
Review
Biotechnology & Applied Microbiology
Yingsong Wang, Zeran Bian, Yan Wang
Summary: As a complex microbial aggregate, biofilm is a group behavior of bacterial ability to adapt to the environment. Bacteria produce biofilm substrates that enhance their tolerance to stress and cause microbial infections. Biofilm infection is usually closely related to virulence, pathogenicity, and even life-threatening to immunocompromised patients. Therefore, studying bacterial biofilm generation and regulatory mechanisms has become one of the most important fields. It is well known that biofilm formation involves group behavior and relies on complex regulation of quorum sensing (QS). A series of small molecule compounds such as indole, AI-2 (autoinducer-2), AHL (N-acyl-homoserine lactone), AIP (auto-inducing peptide), and DSF (diffusible signal factor) are widely available intraspecific or interspecific signaling molecules, with regulatory functions on a wide range of physiological activities of bacteria, including biofilm formation. Given that various bacteria employ QS mechanisms to regulate biofilm formation, inhibition of QS becomes a promising potential strategy for the treatment of bacterial infections. Here, we describe how bacterial intraspecific and interspecific signaling molecules regulate the mechanism of biofilm formation and dispersion. This may contribute to anti-biofilm active molecules and provide ideas or directions for studies on controlling bacterial infections by inhibiting biofilm formation through QS.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Huicong Yan, Congcong Liu, Wentao Yu, Xiaomin Zhu, Baoliang Chen
Summary: Biochar enhances microbial cell to cell communication and biofilm formation through quorum sensing (QS) and provides habitats for soil microbes, thereby shaping soil microbial community structure.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Food Science & Technology
Yibin Xu, Yuanyuan Wang, Xiaoqing Ding, Jiangshui Wang, Xiuan Zhan
Summary: This study demonstrates the antibacterial and antibiofilm properties of reuterin derived from L. reuteri strains. Reuterin exerts its bactericidal activity through ROS production. Furthermore, co-cultures with glycerol enhance the inhibitory effects of LR 21 on Cp 13124, reducing bacterial count and the production of virulence factors.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Jing Dong, Defu Zhang, Jianrong Li, Yongtao Liu, Shun Zhou, Yibin Yang, Ning Xu, Qiuhong Yang, Xiaohui Ai
Summary: Genistein can reduce the virulence of A. hydrophila by decreasing the production of aerolysin and biofilm formation, significantly improving aerolysin-mediated cell injury and providing remarkable protection to channel catfish infected with A. hydrophila. Targeting quorum sensing and virulence can be a useful approach developing drugs against A. hydrophila infections in aquaculture, with genistein as a promising candidate.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Majid Rasool Kamli, Maqsood Ahmad Malik, Vartika Srivastava, Jamal S. M. Sabir, Ehab H. Mattar, Aijaz Ahmad
Summary: This study involves the inexpensive, eco-friendly, and simple green synthesis of ZnO nanoparticles using Origanum vulgare extract, which exhibit antibacterial and quorum sensing inhibition activities against bacteria.
Article
Microbiology
Dan Yang, Suqi Hao, Ling Zhao, Fei Shi, Gang Ye, Yuanfeng Zou, Xu Song, Lixia Li, Zhongqiong Yin, Xiaoli He, Shiling Feng, Helin Chen, Yu Zhang, Yuanze Gao, Yinglun Li, Huaqiao Tang
Summary: The study found that paeonol has good anti-biofilm activity against Gram-negative bacteria and anti-QS activity, effectively reducing the virulence of P. aeruginosa. In vivo studies showed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Zhuqiu Sun, Jinying Xi, Marvin Yeung, Lichao Lu
Summary: This study investigated the effects of two quorum sensing (QS) enhancement methods on the performance and biofilm of biofilters treating chlorobenzene. The results showed that adding exogenous AHLs had a more significant enhancing effect on biofilm, while AHLs producing bacteria helped improve bacterial community diversity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Huan Chen, Cheng-Hai Yan, Yu-Fan Zhan, Li-Tian Geng, Lin-Lin Zhu, Lu-Chan Gong, Jun Wang
Summary: Boron derivatives promote biofilm formation in E. coli by enhancing AI-2 activity, increasing EPS secretion, and upregulating the expression levels of biofilm-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Microbiology
Ronit Vogt Sionov, Doron Steinberg
Summary: Biofilm formation plays a crucial role in chronic and recurrent bacterial infections, protecting the embedded bacteria from antibiotics and making treatment difficult. Quorum sensing and efflux pump systems are important factors in biofilm formation and antibiotic resistance. Targeting these regulatory elements could be a promising approach to combat antibiotic-resistant biofilm-related bacterial infections.
Article
Biology
Jenna A. Moore-Ott, Selena Chiu, Daniel B. Amchin, Tapomoy Bhattacharjee, Sujit S. Datta
Summary: Bacteria are present everywhere in our daily lives, either as mobile planktonic cells or as immobilized surface-attached biofilms. Understanding the conditions under which bacteria transition between these different states is crucial. This study develops a biophysical model to predict and control biofilm formation based on factors such as cell concentration, motility, nutrient diffusion, chemotactic sensing, and autoinducer production.
Article
Infectious Diseases
Dinusha Chathurangi Rajapaksha, Shan Lakmal Edirisinghe, Chamilani Nikapitiya, Ilson Whang, Mahanama De Zoysa
Summary: In this study, it was found that the antimicrobial peptide octopromycin can inhibit the biofilm and quorum-sensing of Acinetobacter baumannii. Octopromycin reduced biofilm mass, caused a collapse in biofilm architecture, and successfully killed antibiotic-resistant persister cells. It also inhibited violacein production in Chromobacterium violaceum and alginate production, surface movements, and twitching motility of A. baumannii.
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.