Journal
FOOD CHEMISTRY
Volume 271, Issue -, Pages 148-156Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.07.115
Keywords
Ganoderma lucidum; Triterpenoids; Hepatoprotection; iTRAQ; Alcohol-induced liver injury
Funding
- Key Project of Fuzhou Municipal Bureau of Science and Technology [2017-N-36]
- Science and Technology Major Project [2014NZ0002-1]
- Natural Science Foundation of Fujian Province, China [2016J06009]
- International Science and Technology Cooperation Project of Fujian Agriculture and Forestry University [KXb16011A]
- Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae Grant [2017FZSK05]
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The hepatoprotective activity of ethanol extract of Ganoderma lucidum (GLE) in alcohol-induced liver injury in mice was investigated. HPLC coupled with photo-diode array detector and electrospray ionization-mass spectrometry was used to analyze the major triterpenoids in GLE. The effects of GLE on hepatoprotection were evaluated through histopathology and biochemical analysis of serum enzymes. We used isobaric tag for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry to identify differentially expressed liver proteome in mice. There were more than 4000 differentially expressed proteins; 40 proteins with the most significant changed proteins were applied for further bioinformatics analysis. Expression levels of cytochrome P450 2E1 and alcohol dehydrogenase 1, proteins that are closely associated with these processes, were validated by western blotting. Triterpenoids, major components of GLE, protected alcohol-induced liver injury through inhibiting lipid peroxidation, elevating activity of antioxidant enzymes, and suppressing apoptotic cell death and immune inflammatory response.
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