Journal
FOOD CHEMISTRY
Volume 244, Issue -, Pages 224-231Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.10.045
Keywords
Crab; Crustacean; qPCR; Real-time PCR; Allergen; Mitochondria; 12S rRNA gene; Internal amplification control
Funding
- United States Food and Drug Administration
Ask authors/readers for more resources
A real-time PCR assay was developed for detection of crab, a crustacean allergen, in food products. Group-specific primers and probes were developed to detect numerous species of crab. Method validation included tests of detection in complex food matrices, evaluation of commercial food products, and cross-reactivity testing on a wide variety of crustaceans. The method was able to detect several species of crab spiked into complex food matrices at levels ranging from 0.1 to 10(5) parts per million (weight/weight), worked equally well on different platforms, exhibited high specificity for crab over other types of crustaceans, and yielded much higher signals from commercial food products listing crab as an ingredient than from those containing other crustaceans.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available