Article
Chemistry, Analytical
Julieth G. Herrera, Marilia P. Ramos, Bheatriz Nunes de Lima Albuquerque, Julio Cesar Ribeiro de Oliveira Farias de Aguiar, Afonso Cordeiro Agra Neto, Patricia Maria Guedes Paiva, Daniela Maria do Amaral Ferraz Navarro, Licarion Pinto
Summary: This study evaluated the effects of temperature, pressure, extraction time, and their interactions on the constituents of essential oil extracted from Piper cor-covadensis using supercritical fluid extraction (SFE). The results showed that pressure and temperature significantly influenced the extraction of constituents, while extraction time was not within the analyzed range. ANOVA-PCA was used to assess the influence of process parameters on each constituent, and the advantages of both ANOVA and univariate ANOVA were compared. Additionally, new constituents were identified during the SFE process.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Toshinobu Hondo, Chihiro Ota, Yumi Miyake, Hiroshi Furutani, Michisato Toyoda
Summary: PTR-MS is a real-time method used to detect trace VOCs in gaseous samples and is popular in various research fields. Supercritical fluid chromatography offers high separation efficiency and easy optimization. Combining PTR with SFC-MS interface allows for detection of nonvolatile organic compounds.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Tatana Gazarkova, Katerina Plachka, Frantisek Svec, Lucie Novakova
Summary: Supercritical fluid chromatography-mass spectrometry (SFC-MS) is a commonly used analytical method that has wide applicability in various fields. However, there are challenges and principles in selecting suitable interfaces and mass analyzers. Additionally, differences arise when using SFC-MS compared to liquid chromatography with atmospheric pressure ionization sources.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yingping Dai, Dasen Jiang, Xinhe Wang, Di Wu, Wenwen Dong, Qing Fu, Yu Jin, Xinmiao Liang
Summary: In this study, quality evaluations of Hedyotis diffusa (H. diffusa) batches were conducted using rapid fingerprint analysis based on supercritical fluid chromatography (SFC). The results showed that the SFC method is feasible for batch quality evaluation of H. diffitsa, as it allows for the effective extraction and separation of chemical components in H. diffusa. The fingerprint analysis revealed quality differences of H. diffusa based on origin and harvest year.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Applied
Peilin Guo, Xiaokang An, Wuying Chen, Xinglu Pan, Runan Li, Jun Xu, Xiaohu Wu, Yongquan Zheng, Fengshou Dong
Summary: In this study, fluindapyr enantiomers were successfully separated by supercritical fluid chromatography-mass spectrometry, and it was found that the enantiomers exhibited different degradation behavior in different plants.
Article
Chemistry, Applied
Xiaoxue Hu, Charfedinne Ayed, Jianshe Chen, Ian Fisk, Ni Yang
Summary: Capsaicin enhances saliva production and affects the interaction between specific aroma compounds and salivary proteins.
Article
Food Science & Technology
Giovanna Cafeo, Antonella Satira, Marina Russo, Monica Mondello, Paola Dugo
Summary: This research aimed to determine oxygen heterocyclic compounds in twenty-six Citrus- and cinnamon-flavoured foods using supercritical fluid chromatography in combination with triple-quadrupole mass spectrometry. The presence of these compounds in all samples analyzed confirmed the use of Citrus fruits in the preparation of the Citrus-flavoured foods. Mandarin juice was found to be the richest source of bioactive compounds, specifically polymethoxyflavones.
Article
Chemistry, Multidisciplinary
Peiling Wu, Le Sun, Shunyao Qi, Tiexin Zeng, Ziyu Hou, Yun Yang, Lijia Xu, Jianhe Wei, Peigen Xiao
Summary: In this study, agarwood leaf aromatic oil was extracted using supercritical carbon dioxide (SC-CO2) extraction and compared with traditional hydrodistillation (HD) and Soxhlet extraction (SE) methods. The results showed that SC-CO2 extraction had higher yields, better smell, and appearance compared to the other two methods. The analysis identified the constituents of the aromatic oil and revealed its antioxidant, anti-inflammatory, and anti-melanogenesis activities. This work demonstrates the great potential of SC-CO2-extracted agarwood leaf aromatic oil as a food additive or dietary supplement.
JOURNAL OF CO2 UTILIZATION
(2023)
Article
Chemistry, Analytical
Barbara Benedetti, Jose Sanchez del Pulgar, Gabriella Di Lena, Ginevra Lombardi-Boccia
Summary: An optimized HPLC-APCI-MS method was developed for analyzing bioactive compounds in lipid matrices, achieving a balance between peak resolution and speed within a 25-minute run. The use of multiple reaction monitoring mode helped overcome coelution issues and improve sensitivity in the analysis.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Fei Yang, Yusong Chen, Jun Liu, Yuan Ji, Kang Wang, Xingyi Jiang, Ying Wang, Shanshan Liu, Ziyan Fan, Zhaoyang Bian, Gangling Tang, Wei Xiong
Summary: Supercritical fluid chromatography tandem mass spectrometry (SFC-MS/MS) is an important tool for analyzing stereoisomers in complex matrices. This study investigated the influence of matrix effect on stereoselective analysis of mevinphos in tobacco by SFC-MS/MS. Experiments showed that a QuEChERS method combined with a dilution strategy effectively eliminated most of the matrix effects.
MICROCHEMICAL JOURNAL
(2022)
Review
Chemistry, Analytical
Marion Burlet-Parendel, Karine Faure
Summary: The emergence of two-dimensional liquid chromatography has facilitated the analysis of complex samples. The review discusses the advantages and challenges of combining liquid chromatography with supercritical fluid chromatography for separating ionizable and neutral solutes, highlighting the orthogonality it offers but also addressing issues such as injection effects in SFC and instrumental limitations that still need to be overcome. The development of comprehensive LC x SFC combination requires collaborative efforts from researchers and instrumental suppliers.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Qing Fu, Wenwen Dong, Dandan Ge, Yanxiong Ke, Yu Jin
Summary: This study developed a method based on simultaneous supercritical fluid extraction-sample cleanup, followed by supercritical fluid chromatography/tandem mass spectrometry (SFE-SFC-MS/MS) for the extraction and analysis of alkaloids, specifically indole alkaloids of Uncaria rhynchophylla. By using a two-step SFE method with the addition of adsorbents, the specificity of extraction towards alkaloids was greatly improved. The SFE extract was then analyzed using SFC-MS/MS, and the results confirmed the presence of indole alkaloids. This method is important for the study of alkaline compounds in complex samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Chemistry, Analytical
Quentin Gros, Johanna Duval, Caroline West, Eric Lesellier
Summary: The development of automatized hyphenated systems is attractive for avoiding sampling error, saving time, and reducing costs. Among these systems, on-line SFE-SFC allows efficient, quick, and eco-friendly extraction, separation, and detection of molecules. The principles of supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) are discussed, along with comparisons of partially-supercritical on-line systems before examining the evolution of on-line SFE-SFC over the past decades. Different devices for better understanding coupling challenges and determining advantageous configurations are also examined.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yih Ling Saw, John R. Boughton, Faith L. Wroniuk, Mahmoud Elhusseiny Mostafa, Peter J. Pellegrinelli, Samantha A. Calvez, Alexander S. Kaplitz, Lark J. Perez, James L. Edwards, James P. Grinias
Summary: This report explores the use of a high proton affinity N-(4-aminophenyl)piperidine derivatization tag to improve the detection of organic acids by SFC-MS. By derivatizing four organic acids with varying carboxylate groups, detection limits as low as 0.5 ppb were achieved, with overall improvements in detection limit ranging from 25-to-2100-fold. The effect of derivatization on sensitivity, and the possibility of complete separation of these derivatized compounds using SFC, were also described.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Adriana Arigo, Marina Russo, Maria Rita Testa Camillo, Paola Dugo, Luigi Mondello, Mariosimone Zoccali
Summary: Oxygen heterocyclic compounds are beneficial secondary metabolites present in cold-pressed Citrus essential oils. An analytical approach based on supercritical fluid chromatography coupled to tandem mass spectrometry was validated for the analysis of these compounds. The method showed good separation and sensitivity, with accurate quantification. The study provides quantitative profiles of different Citrus essential oils, highlighting their content of oxygen heterocyclic compounds.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Imad Kabach, Najat Bouchmaa, Reda Ben Mrid, Zakia Zouaoui, Mohammed El Maadoudi, Ayoub Kounnoun, Fadoua Asraoui, Fouad El Mansouri, Abdelmajid Zyad, Francesco Cacciola, Yassine Oulad El Majdoub, Luigi Mondello, Mohamed Nhiri
Summary: This study investigated the phytochemical composition, antioxidant properties, antidiabetic potential, and cytotoxicity of Olea europaea var. oleaster (Oeo) fruit. The results showed that Oeo fruit contains high levels of phenolic and flavonoid compounds and exhibits strong antioxidant activity. Methanolic extract demonstrated better antioxidant activity than aqueous extract and showed inhibitory effects on glycation and breast cancer cells.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Chemistry, Applied
Mustapha Cherrate, Reda EL-Mernissi, Aziz Bouymajane, Fouzia Rhazi Filali, Emanuela Trovato, Ghizlane Echchgadda, Abdellah Maissour, Mohammed Aziz Ajana, Paola Dugo, Francesco Cacciola, Luigi Mondello, Kacem Makroum
Summary: The study focused on characterizing the chemical composition and assessing the antioxidant and antibacterial activities of Ziziphora hispanica essential oil (ZH-EO) from the Middle Atlas of Morocco (Boulmane). Results showed that ZH-EO consisted of 119 volatile components, representing 88.7% of the total oil. ZH-EO exhibited bactericidal and bacteriostatic effects against various bacteria and demonstrated strong antioxidant activity. ADMET prediction indicated favorable pharmacokinetic properties of the tested components. This study suggests that Ziziphora hispanica essential oil could be a potential source of bioactive molecules for preventing diseases related to oxidative stress and pathogenic bacteria.
JOURNAL OF ESSENTIAL OIL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nadia Naim, Aziz Bouymajane, Yassine Oulad El Majdoub, Said Ezrari, Rachid Lahlali, Abdessalem Tahiri, Said Ennahli, Roberto Lagana Vinci, Francesco Cacciola, Luigi Mondello, Ilham Madani
Summary: This study investigates the phenolic composition and antibacterial properties of hydroethanolic extracts from Crocus sativus L. petals collected in Serghina, Morocco. The extracts were found to contain 27 phenolic compounds, with the most abundant being kaempferol-sophoroside isomer. The extracts showed bactericidal effects against Staphylococcus aureus and Listeria monocytogenes, and bacteriostatic effects against Escherichia coli and Salmonella typhimurium. These findings suggest that Crocus sativus L. petal extracts could be valuable sources of natural antibacterial agents for the food and pharmaceutical industries.
Article
Food Science & Technology
Ghanya Al-Naqeb, Cinzia Cafarella, Eugenio Aprea, Giovanna Ferrentino, Alessandra Gasparini, Chiara Buzzanca, Giuseppe Micalizzi, Paola Dugo, Luigi Mondello, Francesca Rigano
Summary: This study evaluated the potential of supercritical fluid extraction (SFE) as a green alternative to Soxhlet extraction for obtaining oils from Opuntia ficus-indica (OFI) and Opuntia dillenii (OD) seeds. SFE with specific parameters produced oils rich in unsaturated fatty acids and tocopherols. The highest yield was achieved through Soxhlet extraction, and the most abundant fatty acid in all samples was linoleic acid. SFE of OFI seeds from Yemen resulted in the highest gamma-tocopherol content. Overall, SFE demonstrated its suitability for producing oils with potential applications in functional food and nutraceutical products.
Article
Biochemical Research Methods
Danilo Donnarumma, Alessia Arena, Emanuela Trovato, Francesca Rigano, Mariosimone Zoccali, Luigi Mondello
Summary: In this study, a miniaturized workflow was developed to analyze the different lipid classes and fatty acid constituents in human serum. Gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry were used for quantification and identification of fatty acids and vitamin D metabolites. The combination of targeted and untargeted data provided valuable information for assessing the physiological condition of the organism and tailoring efficient treatment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Tania M. G. Salerno, Emanuela Trovato, Giovanna Cafeo, Federica Vento, Mariosimone Zoccali, Paola Donato, Paola Dugo, Luigi Mondello
Summary: During the Covid-19 pandemic, it was found that some citrus-scented hand gels did not meet the labeling requirements for fragrance allergens, and their furocoumarin content exceeded the safe limits. Additionally, some products did not match the information reported on the label regarding the presence of essential oils. Therefore, urgent development of analytical tools and regulatory actions for widespread testing of hand hygiene products is needed to ensure consumer health and safety.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Imad Kabach, Najat Bouchmaa, Zakia Zouaoui, Abdelhamid Ennoury, Sara El Asri, Abdelmounaim Laabar, Loubna Oumeslakht, Francesco Cacciola, Yassine Oulad El Majdoub, Luigi Mondello, Abdelmajid Zyad, Naima Nhiri, Mohamed Nhiri, Reda Ben Mrid
Summary: The study aimed to evaluate the antioxidant and antidiabetic properties of Oxalis pes-caprae flowers extract in alloxan-induced diabetic mice. Results showed that the extract exhibited significant antioxidant and antidiabetic activity by reducing blood glucose levels and increasing the activities of antioxidants and glycolysis enzymes.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Paola Arena, Natalizia Miceli, Andreana Marino, Federica Davi, Emilia Cavo, Vivienne Spadaro, Francesco Maria Raimondo, Francesco Cacciola, Roberto Lagana Vinci, Luigi Mondello, Maria Fernanda Taviano
Summary: This study aimed to explore Sinapis pubescens subsp. pubescens as a new potential source of active metabolites. Comparative analysis was conducted on the hydroalcoholic extracts of the leaf, flower, and stem. Polyphenols in the extracts were quantitatively determined and characterized, revealing significant differences in their profiles. The extracts exhibited antioxidant activity, with the leaf extract showing the best radical scavenging activity and the flower extract demonstrating the highest chelating activity. However, no antimicrobial activity was observed in the strains tested. Moreover, the extracts were found to be non-toxic. The aerial parts of S. pubescens subsp. pubescens could serve as valuable sources of antioxidants for pharmaceutical and nutraceutical applications.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Food Science & Technology
Emanuela Trovato, Marina Russo, Lorenzo Cucinotta, Yassine Oulad El Majdoub, Maria Rita Testa Camillo, Gemma De Grazia, Adriana Arigo, Danilo Sciarrone, Luigi Mondello, Paola Dugo
Summary: Flavoured EVOOs in the niche market of extra-virgin olive oil are enriched with aromatic and medicinal plants, which affect their chemical composition. Analytical techniques including HPLC and GC methods were used to investigate the qualiquantitative profile of molecules in the volatile and non-volatile fractions of flavoured EVOOs. The results confirmed the genuineness of most flavoured EVOOs, with a few requiring additional analytical devices for further authentication.
FOOD ANALYTICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Federica Davi, Maria Fernanda Taviano, Rosaria Acquaviva, Giuseppe Antonio Malfa, Emilia Cavo, Paola Arena, Salvatore Ragusa, Francesco Cacciola, Yassine Oulad El Majdoub, Luigi Mondello, Natalizia Miceli
Summary: Our research team conducted a study on Brassica fruticulosa subsp. fruticulosa, an edible plant used for treating various ailments. We found that the leaf extract exhibited good antioxidant properties, with the secondary compounds being more potent than the primary ones. Further investigation revealed that the phenolic compounds in the extract possessed antioxidant properties and inhibited the proliferation of human colorectal epithelial adenocarcinoma cells.
Article
Food Science & Technology
Ahlem Eddehech, Renaud Rahier, Danilo Donnarumma, Francesca Rigano, Alexandre Noiriel, Abdelkarim Abousalham, Francesco Cacciola, Luigi Mondello, Zied Zarai
Summary: This study presents the expression and characterization of a novel phosphatidylinositol phospholipase C (PI-PLCBt) from Bacillus thuringiensis PL14, which shows potential for oil degumming. Enzymatic degumming tests were conducted on crude soybean oil using rPI-PLCBt, and its efficiency was evaluated by measuring phosphorus and diacylglycerol (DAG) contents after different treatments. The results showed that enzymatic degumming achieved a high efficiency of 89.4%, reducing phosphorus content to 13.7 mg kg-1 and increasing DAG levels. This suggests that enzymatic degumming could be a more efficient and environmentally friendly alternative to traditional degumming methods, while also enhancing the nutritional value of the final product.
Article
Integrative & Complementary Medicine
Csongor Bako, Viktoria Lilla Balazs, Erika Kerekes, Bela Kocsis, David U. Nagy, Peter Szabo, Giuseppe Micalizzi, Luigi Mondello, Judit Krisch, Dora Petho, Gyorgyi Horvath
Summary: Thyme essential oil is popular due to its antimicrobial effect, but the chemical composition of the oil influences its biological activity. This study investigates the influence of flowering phenophases on the chemical composition, antibacterial and anti-biofilm activity of Thymus vulgaris essential oil, finding that the collection time has important implications for its activity.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2023)
Article
Chemistry, Analytical
Javier Garcia-Bellido, Laura Freije-Carrelo, Montserrat Redondo-Velasco, Marco Piparo, Mariosimone Zoccali, Luigi Mondello, Mariella Moldovan, Brice Bouyssiere, Pierre Giusti, Jorge Ruiz Encinar
Summary: In this study, the potential and applicability of a novel GC-combustion-MS approach as a nitrogen-selective GC detector were demonstrated. By adjusting the operating conditions, the formation of volatile NO species during the combustion step was achieved, leading to a selective N-signal. The method showed a very low detection limit (0.02 pg N injected) and provided accurate and precise results.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Natalia Manousi, Antonio Ferracane, Natasa P. Kalogiouri, Abuzar Kabir, Kenneth G. Furton, Peter Q. Tranchida, George A. Zachariadis, Luigi Mondello, Victoria F. Samanidou, Erwin Rosenberg
Summary: In this study, sol-gel sorbent-coated second-generation fabric phase sorptive extraction (FPSE) membranes were synthesized using titania-based sol-gel precursors. These membranes showed high efficiency in extracting organophosphorus pesticides from apple juice samples. The optimized FPSE method using these membranes demonstrated good accuracy in analysis and the novel membranes were reusable up to 5 times. Comparatively, the titania-based membranes provided higher extraction recoveries than conventional silica-based membranes.
Article
Biochemical Research Methods
Alessia Arena, Antonio Ferracane, Mariosimone Zoccali, Markus Obkircher, Hanspeter Sprecher, Peter Q. Tranchida, Luigi Mondello
Summary: This study presents a rapid and limited-volume solvent-extraction sample preparation method for pesticide determination in hemp seed oil. Compared to previous research, this study extends the range of pesticides and investigates a more challenging matrix.
JOURNAL OF CHROMATOGRAPHY A
(2023)
