Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Medicine, General & Internal
Jing Wang, Junwen Wang, Xinyan Li, Kai Shu
Summary: Traumatic brain injury (TBI) is a complex and life-threatening condition with no effective treatment to date. Exosomes (Exos), particularly those derived from cells, have the potential to deliver drugs to target lesions in the brain by crossing the blood-brain barrier. Moreover, Exos-derived microRNAs show promise as biomarkers for TBI. This review examines the potential impact of cell-derived Exos and exosome-derived microRNAs on the diagnosis, therapy, and prognosis prediction of TBI.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Anuvab Dey, Subhrojyoti Ghosh, Tiyasa Bhuniya, Madhurima Koley, Aishi Bera, Sudeepta Guha, Kashmira Chakraborty, Sathish Muthu, Sukhamoy Gorai, Rany Vorn, Chithravel Vadivalagan, Krishnan Anand
Summary: Traumatic brain injury (TBI) is a common cause of disability and fatality worldwide. Accurate early diagnosis can strongly impact the patient's discomfort, prognosis, therapeutic approach, survival rates, and recurrence. Brain-derived extracellular vesicles (EVs) have a specific screening role in TBI research, and their therapeutic impact shows promise for aiding in TBI healing. This review highlights the relationships between EVs and TBI theranostics, EVs and TBI-related clinical trials, and the challenges and solutions in the research domain.
ACS CHEMICAL NEUROSCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Ines Dinis Aires, Teresa Ribeiro-Rodrigues, Raquel Boia, Magda Ferreira-Rodrigues, Henrique Girao, Antonio Francisco Ambrosio, Ana Raquel Santiago
Summary: Microglial cells are important for maintaining neuronal homeostasis in the central nervous system, while chronic neuroinflammation plays a role in neurodegenerative diseases. Extracellular vesicles, such as apoptotic bodies, microvesicles, and exosomes, are involved in intercellular communication and may regulate microglial cell function. Understanding the role of microglial extracellular vesicles could provide insights into the mechanisms underlying neurodegeneration.
Review
Biochemistry & Molecular Biology
Katarzyna Horodecka, Markus Duchler
Summary: The establishment of CRISPR/Cas9 technology has revolutionized gene editing and therapeutic interventions, but challenges remain in effectively delivering components to living organisms. Utilizing extracellular vesicles as delivery devices may help overcome limitations and improve the clinical applications of this technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Chang Tian, Ke Wang, Min Zhao, Shan Cong, Xin Di, Ranwei Li
Summary: Sepsis, a life-threatening condition caused by a dysregulated host response to infection, is a major cause of mortality worldwide. Early diagnosis and effective treatment of sepsis remain challenging due to its rapid progression and heterogeneity among individuals. This review highlights the role of extracellular vesicles (EVs) in sepsis, including their impact on pathological processes, organ dysfunction, and potential applications such as early diagnosis, dynamic monitoring, precision therapies, and vaccine platforms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Medicine, Research & Experimental
Artur Slomka, Bingduo Wang, Tudor Mocan, Adelina Horhat, Arnulf G. Willms, Ingo G. H. Schmidt-Wolf, Christian P. Strassburg, Maria A. Gonzalez-Carmona, Veronika Lukacs-Kornek, Miroslaw T. Kornek
Summary: Liquid biopsies, as biomarkers in cancer diagnosis, have great potential but also limitations. This review focuses on comparing extracellular vesicles (EVs) and circulating tumor cells (CTCs) and discussing their similarities in characterization and quantification. While it is not concluded which is better between CTCs and EVs, the review provides reasons to intensify research on both.
Review
Biochemistry & Molecular Biology
Marc Liebana-Jordan, Bruno Brotons, Juan Manuel Falcon-Perez, Esperanza Gonzalez
Summary: Extracellular vesicles play a crucial role in intercellular communication, especially in fungi, by maintaining cell structure, defending against the environment, and potentially impacting host interactions and disease outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Wu Zhou, Lihong Zhao, Zelu Mao, Zhihua Wang, Zhixiong Zhang, Meihua Li
Summary: Substances released by the brain can affect other organs, and substances produced by organs like bone marrow or adipose tissue can impact the brain. Research on the function of molecular mediators carried by extracellular vesicles is still in its early stages.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Fei Teng, Martin Fussenegger
Summary: Extracellular vesicles (EVs) are nano-sized secreted vesicles containing various biomolecules, which have low immunogenicity and can functionally modify recipient cells by transferring bioactive constituents. Therefore, they are considered as an excellent candidate for the next generation drug delivery system.
Review
Chemistry, Multidisciplinary
Yujia Hao, Haoyue Song, Zilan Zhou, Xiaohang Chen, Huifei Li, Yuan Zhang, Jie Wang, Xiuyun Ren, Xing Wang
Summary: Extracellular vesicles (EVs) are important vehicles for intercellular communication, with different physiological and pathological effects, and can be used as biological therapeutic agents. In some cases, the release of EVs can exacerbate disease progression, while inhibition may delay disease development.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Cell Biology
Samuel Amintas, Veronique Vendrely, Charles Dupin, Louis Buscail, Christophe Laurent, Barbara Bournet, Jean-Philippe Merlio, Aurelie Bedel, Francois Moreau-Gaudry, Julian Boutin, Sandrine Dabernat, Etienne Buscail
Summary: Extracellular vesicles (EVs) are small vesicles produced by healthy tissues and tumor cells, carrying a rich content of biomolecules, which play important roles in cancer control and progression. They are being studied as carriers of liquid biopsy biomarkers, with double-stranded DNA (dsDNA) in EVs showing potential as a promising biomarker for tumor presence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Evelyn Lattmann, Mitchell P. Levesque
Summary: Melanoma, the deadliest form of skin cancer, can be diagnosed and treated using extracellular vesicles as biomarkers, which also reveal important signaling pathways for cancer progression.
Review
Cell Biology
Sarah Beck, Bernhard Hochreiter, Johannes A. Schmid
Summary: Extracellular vesicles (EVs) play crucial roles in intercellular communication by transferring nucleotides and proteins, and are associated with diseases such as cancer, inflammation, and thrombosis. They have the potential to serve as biomarkers for diagnosis and as vehicles for therapeutics in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Amit Chatterjee, Ruchira Singh
Summary: Extracellular vesicles (EVs) are secreted membrane vesicles that come in various sizes, including exosomes (-30-200 nm) and microvesicles (MVs) that are around 100-1,000 nm in size. These EVs have been found to play a crucial role in autocrine, paracrine, and endocrine signaling, and are associated with a range of human disorders, including retinal degenerative diseases such as age related macular degeneration (AMD) and diabetic retinopathy (DR). In this review, the authors provide an overview of the current understanding of the role of EVs in retinal physiology and specifically focus on how disease-related changes in EVs can impact specific retinal diseases. Additionally, they discuss the potential of EVs in diagnostic and therapeutic strategies for targeting retinal diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Spectroscopy
Wenjing Peng, Cristian D. Gutierrez Reyes, Sakshi Gautam, Aiying Yu, Byeong Gwan Cho, Mona Goli, Kaitlyn Donohoo, Stefania Mondello, Firas Kobeissy, Yehia Mechref
Summary: Glycosylation is a significant posttranslational modification in mammalian cells, involved in various biofunctions and diseases. Mass spectrometry is a powerful tool for glycan and glycopeptide isomeric analysis, but its comprehensive characterization remains a challenge. Separation methods and bioinformatic tools are applied to address this challenge.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Spectroscopy
Khalil Mallah, Kazem Zibara, Coline Kerbaj, Ali Eid, Nour Khoshman, Zahraa Ousseily, Abir Kobeissy, Tristan Cardon, Dasa Cizkova, Firas Kobeissy, Isabelle Fournier, Michel Salzet
Summary: Traumatic brain injury (TBI) is a major public health concern worldwide, and the use of proteomic and lipidomic platforms has broadened our understanding of TBI-related mechanisms and neuropathological complications. This study provides an updated overview of spatially resolved microproteomics and microlipidomics approaches guided by mass spectrometry imaging in TBI studies and discusses their applications in neurotrauma research. The accurate and precise techniques of microproteomic sampling, such as laser capture microdissection, parafilm assisted microdissection, and liquid microjunction extraction, are evaluated. The potential of lipid profiling, particularly in phospholipid metabolism and proinflammatory molecules, is highlighted.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Critical Care Medicine
Carleen Batson, Logan Froese, Mypinder Sekhon, Donald Griesdale, Alwyn Gomez, Eric P. Thelin, Rahul Raj, Marcel Aries, Clare Gallagher, Francis Bernard, Andreas H. Kramer, Frederick A. Zeiler
Summary: This study investigates the impact of age and biological sex on cerebral physiology and finds a positive linear correlation between PAx, RAC, and age, but no relationship between PRx and age.
JOURNAL OF NEUROTRAUMA
(2023)
Article
Critical Care Medicine
Alexander Fletcher-Sandersjoo, Charles Tatter, Jonathan Tjerkaski, Jiri Bartek, Marc Maegele, David W. Nelson, Mikael Svensson, Eric Peter Thelin, Bo-Michael Bellander
Summary: This study used 3D volume rendering to determine the expansion of traumatic intracranial hematomas over time and evaluate its impact on outcome. The findings suggest that hematoma expansion is a driver of unfavorable outcome in traumatic brain injury, and even small changes in hematoma volume can affect functional outcome. The study also proposes a wider window of opportunity to prevent lesion progression than what has previously been suggested.
NEUROCRITICAL CARE
(2023)
Review
Public, Environmental & Occupational Health
Periklis Charalampous, Juanita A. Haagsma, Lea S. Jakobsen, Vanessa Gorasso, Isabel Noguer, Alicia Padron-Monedero, Rodrigo Sarmiento, Joao Vasco Santos, Scott A. McDonald, Dietrich Plass, Grant M. A. Wyper, Ricardo Assuncao, Elena von der Lippe, Balazs Adam, Ala'a AlKerwi, Jalal Arabloo, Ana Lucia Baltazar, Boris Bikbov, Maria Borrell-Pages, Iris Brus, Genc Burazeri, Serafeim C. Chaintoutis, Jose Chen-Xu, Nino Chkhaberidze, Seila Cilovic-Lagarija, Barbara Corso, Sarah Cuschieri, Carlotta Di Bari, Keren Dopelt, Mary Economou, Theophilus Emeto, Peter Fantke, Florian Fischer, Alberto Freitas, Juan Manuel Garcia-Gonzalez, Federica Gazzelloni, Mika Gissler, Artemis Gkitakou, Hakan Gulmez, Sezgin Gunes, Sebastian Haller, Romana Haneef, Cesar A. Hincapie, Paul Hynds, Jane Idavain, Milena Ilic, Irena Ilic, Gaetano Isola, Zubair Kabir, Maria Kamusheva, Pavel Kolkhir, Naime Meric Konar, Polychronis Kostoulas, Mukhtar Kulimbet, Carlo La Vecchia, Paolo Lauriola, Miriam Levi, Marjeta Majer, Enkeleint A. Mechili, Lorenzo Monasta, Stefania Mondello, Javier Munoz Laguna, Evangelia Nena, Edmond S. W. Ng, Paul Nguewa, Vikram Niranjan, Iskra Alexandra Nola, Ronan O'Caoimh, Marija Obradovic, Elena Pallari, Mariana Peyroteo, Vera Pinheiro, Nurka Pranjic, Miguel Reina Ortiz, Silvia Riva, Cornelia Melinda Adi Santoso, Milena Santric Milicevic, Tugce Schmitt, Niko Speybroeck, Maximilian Sprugel, Paschalis Steiropoulos, Aleksandar Stevanovic, Lau Caspar Thygesen, Fimka Tozija, Brigid Unim, Hilal Bektas Uysal, Orsolya Varga, Milena Vasic, Rafael Jose Vieira, Vahit Yigit, Brecht Devleesschauwer, Sara M. Pires
Summary: This systematic literature review provides an overview of studies applying the disability-adjusted life years (DALY) concept for infectious diseases in European countries. Among the 105 included studies, food- and water-borne diseases were the most frequently studied. The number of burden of infectious disease studies has increased over time, but there is less consensus in methodological approaches.
EPIDEMIOLOGY AND INFECTION
(2023)
Review
Psychology, Clinical
Jana El Husseiny, Sarah H. H. Farran, Walaa G. G. El Sheikh, Yara Yehya, Samer El Hayek, Hussein El Husseiny, Layal Hneiny, Shima Shahjouei, Mirna Sabra, Firas Kobeissy
Summary: This systematic review examines PTSD in Lebanon and among Syrian refugees and finds a high prevalence of the disorder in these populations, particularly related to war events. The study emphasizes the importance of developing treatment strategies and improving management.
PSYCHOLOGICAL TRAUMA-THEORY RESEARCH PRACTICE AND POLICY
(2023)
Review
Neurosciences
Maya Jammoul, Dareen Jammoul, Kevin K. Wang, Firas Kobeissy, Ralph G. Depalma
Summary: This article reviews the possible mechanisms by which traumatic brain injury (TBI) may stimulate the development of opioid use disorder (OUD) and discusses the interaction between these two processes. CNS damage due to TBI appears to drive adverse effects of subsequent OUD, with pain being a risk factor for opioid use after TBI.
BIOLOGICAL PSYCHIATRY
(2024)
Article
Biochemistry & Molecular Biology
Akeem Sanni, Mona Goli, Jingfu Zhao, Junyao Wang, Chloe Barsa, Samer El Hayek, Farid Talih, Bartolo Lanuzza, Firas Kobeissy, Giuseppe Plazzi, Monica Moresco, Stefania Mondello, Raffaele Ferri, Yehia Mechref
Summary: This study aimed to identify potential serum biomarkers for narcolepsy type 1 (NT1), a life-threatening disorder characterized by a sleep-wake cycle disorder. A proteomics analysis was conducted, which revealed 36 differentially expressed proteins associated with NT1. These findings provide insights into the pathophysiological effects and important biochemical pathways involved in NT1.
Editorial Material
Clinical Neurology
Wael M. Y. Mohamed, Firas Kobeissy, Stefania Mondello
FRONTIERS IN NEUROLOGY
(2023)
Article
Public, Environmental & Occupational Health
Sara Conti, Carla Fornari, Pietro Ferrara, Ippazio C. Antonazzo, Fabiana Madotto, Eugenio Traini, Miriam Levi, Achille Cernigliaro, Benedetta Armocida, Nicola L. Bragazzi, Ennio Cadum, Michele Carugno, Giacomo Crotti, Silvia Deandrea, Paolo A. Cortesi, Davide Guido, Ivo Iavicoli, Sergio Iavicoli, Carlo La Vecchia, Paolo Lauriola, Paola Michelozzi, Salvatore Scondotto, Massimo Stafoggia, Francesco S. Violante, Cristiana Abbafati, Luciana Albano, Francesco Barone-Adesi, Antonio Biondi, Cristina Bosetti, Danilo Buonsenso, Giulia Carreras, Giulio Castelpietra, Alberico Catapano, Maria S. Cattaruzza, Barbara Corso, Giovanni Damiani, Francesco Esposito, Silvano Gallus, Davide Golinelli, Simon I. Hay, Gaetano Isola, Caterina Ledda, Stefania Mondello, Paolo Pedersini, Umberto Pensato, Norberto Perico, Giuseppe Remuzzi, Francesco Sanmarchi, Rocco Santoro, Biagio Simonetti, Brigid Unim, Marco Vacante, Massimiliano Veroux, Jorge H. Villafane, Lorenzo Monasta, Lorenzo G. Mantovani
Summary: We investigated the temporal variations in disease burden of PM2.5 and ozone in Italy using data from the Global Burden of Disease Study 2019. The study found that while the burden of PM2.5 significantly decreased in Italy, the burden of ozone increased, even in the context of population aging.
INTERNATIONAL JOURNAL OF PUBLIC HEALTH
(2023)
Article
Clinical Neurology
I-Hsuan Lin, Alaa Kamnaksh, Roxanne Aniceto, Jesse McCullough, Ramsey Bekdash, Michael Eklund, Per Hamid Ghatan, Marten Risling, Mikael Svensson, Bo-Michael Bellander, David W. W. Nelson, Eric Peter Thelin, Denes V. V. Agoston
Summary: Monitoring protein biomarker levels in the cerebrospinal fluid can help assess traumatic brain injury severity and outcome. This study compared the changes in S100B, NSE, Tau, and p-Tau levels in CSF and bECF samples collected from severe TBI patients. The study showed similar trends in biomarker changes between CSF and bECF samples, indicating the importance of both quantitative and qualitative analysis of protein biomarkers.
NEUROTRAUMA REPORTS
(2023)
Article
Multidisciplinary Sciences
Sara Wood, Riccardo Iacobelli, Sarah Kopfer, Caroline Lindblad, Eric Peter Thelin, Alexander Fletcher-Sandersjoo, Lars Mikael Broman
Summary: The risk of ICH is high in neonatal patients treated with ECMO, and lower gestational age and higher pre-ECMO lactate levels are associated with an increased risk of ICH.
SCIENTIFIC REPORTS
(2023)
Review
Oncology
Reem Halabi, Fatima Dakroub, Mohammad Z. Haider, Stuti Patel, Nayef A. Amhaz, Mohammad A. Reslan, Ali H. Eid, Yehia Mechref, Nadine Darwiche, Firas Kobeissy, Ibrahim Omeis, Abdullah A. Shaito
Summary: This review discusses the genetic and epigenetic abnormalities in meningiomas, with a focus on neurofibromatosis type 2 and non-NF2 mutations. It also explores novel biomarkers for meningioma diagnosis and prognosis, including epigenetic-, RNA-, metabolomics-, and protein-based markers. Additionally, the available meningioma-specific animal models are overviewed.
Article
Medicine, General & Internal
Franklin Chu Buh, Irene Ule Ngole Sumbele, Andrew I. R. Maas, Mathieu Motah, Jogi V. Pattisapu, Eric Youm, Basil Kum Meh, Firas H. Kobeissy, Kevin W. Wang, Peter J. A. Hutchinson, Germain Sotoing Taiwe
Summary: The study revealed that TBI in Cameroon is predominantly caused by road traffic incidents and primarily affects young adult males. Lack of pre-hospital care, financial constraints limiting both CT scanning and medical care, and a lack of acute physiotherapy services likely influenced care and outcomes adversely.
MEDICINA-LITHUANIA
(2023)