Article
Biochemistry & Molecular Biology
Rene D. Verboket, Nicolas Soehling, Myriam Heilani, Charlotte Fremdling, Alexander Schaible, Katrin Schroeder, Jan C. Brune, Ingo Marzi, Dirk Henrich
Summary: The Masquelet technique is a two-stage procedure used to treat large bone defects. This study tested the effectiveness of demineralized bone matrix and bone marrow mononuclear cells as membrane fillings, and found that they cannot replicate the good results of this material in large, non-membrane coated bone defects. However, they do support the maturation of new bone tissue locally.
Article
Polymer Science
Chong Teng, Zhicheng Tong, Qiulin He, Huangrong Zhu, Lu Wang, Xianzhu Zhang, Wei Wei
Summary: In this study, biocompatible hydrogel microspheres were fabricated to promote the osteogenic differentiation of stem cells and enhance bone regeneration. The results from in vivo experiments demonstrated the superiority of the BMSC/HM system in inducing bone regeneration. This study provides a simple and effective research idea for bone defect repair, and the optimization of hydrogel microspheres holds promise for future tissue engineering applications.
Article
Cell & Tissue Engineering
Caroline Seebach, Christoph Nau, Dirk Henrich, Rene Verboket, Marlene Bellen, Nadine Frischknecht, Vivien Moeck, Kathrin Eichler, Kay Hajo Schmidt Horlohe, Reinhard Hoffmann, Halvard Bonig, Erhard Seifried, Johannes Frank, Ingo Marzi
Summary: Autologous bone marrow mononuclear cells combined with β-TCP scaffold can reduce the incidence of secondary dislocation in patients with proximal humerus fracture. Comparable results in bone healing and shoulder function were observed compared to traditional surgical treatment. This method may be feasible in other clinical situations as well.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Cheng Su, Xiao Zheng, Yanjin He, Li Long, Wenchuan Chen
Summary: This study identified aberrant expression of circRNAs in hUCMSCs during osteogenic differentiation, constructed competing endogenous RNA networks using bioinformatics tools, elucidated underlying mechanisms, and validated the osteogenic-promoting effect of a specific circRNA.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Physical
Jorge Luis da Silva Pires, Jorge Jose de Carvalho, Mario Jose dos Santos Pereira, Igor da Silva Brum, Ana Lucia Rosa Nascimento, Paulo Goncalo Pinto dos Santos, Lucio Frigo, Ricardo Guimaraes Fischer
Summary: This study compares the use of synthetic hydroxyapatite and xenograft for bone defects in rat calvaria, and found a significant enhancement in new bone matrix formation in groups using biomaterials compared to the control group. However, the addition of bone marrow mononuclear fraction did not further enhance the potential of hydroxyapatite-based biomaterials to induce bone matrix formation.
Article
Pharmacology & Pharmacy
Ilenia Mastrolia, Andrea Giorgini, Alba Murgia, Pietro Loschi, Tiziana Petrachi, Valeria Rasini, Massimo Pinelli, Valentina Pinto, Francesca Lolli, Chiara Chiavelli, Giulia Grisendi, Maria Cristina Baschieri, Giorgio De Santis, Fabio Catani, Massimo Dominici, Elena Veronesi
Summary: In this study, a rabbit model was established to mimic human osteonecrosis of the femoral head (ONFH) and evaluate the autologous MSC-based treatment. Results showed that implantation of autologous BM-MSCs could restore the damaged tissue in rabbit femurs. Furthermore, the study found that osteonecrosis did not affect the behavior of MSCs.
Article
Biotechnology & Applied Microbiology
Yue Ke, Yu Ye, Jintao Wu, Yanxia Ma, Yuxin Fang, Fei Jiang, Jinhua Yu
Summary: A phosphoserine-loaded chitosan membrane was synthesized to improve the osteogenic effect of chitosan membranes. The incorporation of phosphoserine into chitosan membranes was confirmed and the phosphoserine-chitosan membranes significantly stimulated bone regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Nicolas Soehling, Myriam Heilani, Charlotte Fremdling, Alexander Schaible, Katrin Schroeder, Jan C. Brune, Volker Eras, Christoph Nau, Ingo Marzi, Dirk Henrich, Rene D. Verboket
Summary: The classic two-stage masquelet technique is effective for treating large bone defects. Using decellularized dermis membrane (DCD) can save one surgery. It is also possible to use granular demineralized bone matrix (g-DBM) or fibrous demineralized bone matrix (f-DBM) instead of syngeneic cancellous bone (SCB) for defect filling.
Article
Materials Science, Multidisciplinary
Shengqi Zang, Can Xiao, Maodian He, Bo Chen, Bingyao Liu, Shuai Yi, Lei Wang, Junxia Li, Xiaolei Shi, Shoushan Bu, Lei Jin
Summary: Biomimetic surface modification using ZrO2 scaffold with rhBMP-7 and RGD has shown promising results in improving bioactivity and stimulating stem cell signals in bone tissue engineering. The combination of rhBMP-7 and RGD enhanced osteogenic differentiation and in vivo bone formation in critical-sized calvarial bone defects implanted with hDPSCs-seeded ZrO2-RGD-BMP-7 scaffold. These findings support the application of this surface-modifying biomolecule and stem cell-based approach for bone regeneration.
MATERIALS & DESIGN
(2023)
Article
Biotechnology & Applied Microbiology
Long Chen, Wei Luo, Yuanzheng Wang, Xiongbo Song, Senlei Li, Jun Wu, Li Sun
Summary: This study demonstrates that glycosylation modification enhances the directional homing ability of BMSCs to bone defect sites by enabling them to express high levels of sLe(X) antigen. Results show that glycosylation-modified BMSCs can improve the repair of bone defects in mice.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Terumasa Umemoto, Alban Johansson, Shah Adil Ishtiyaq Ahmad, Michihiro Hashimoto, Sho Kubota, Kenta Kikuchi, Haruki Odaka, Takumi Era, Daisuke Kurotaki, Goro Sashida, Toshio Suda
Summary: By studying the mitochondrial metabolism and ATP citrate lyase (ACLY) in hematopoietic stem cells (HSCs), we have gained new insights into the regulation of HSCs during hematopoietic regeneration.
Article
Biotechnology & Applied Microbiology
Lei Qi, Weiwen Ge, Cancan Pan, Weidong Jiang, Dan Lin, Lei Zhang
Summary: Stem cell senescence causes functional declines and disrupts bone environment homeostasis. Stem cell-derived exosomes are a promising therapy for aging-related osseous diseases. This study demonstrates the potential of osteoinductive exosomes in bone repair for aging individuals and highlights the importance of considering endocytic mechanisms and avoiding negative effects in future therapeutic design for aging populations.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Carolina S. Santinoni, Adrieli P. C. Neves, Breno F. M. Almeida, Natalia C. Kajimoto, Natalia M. Pola, Eliana A. Caliente, Eduarda L. G. Belem, Joilson B. Lelis, Stephen E. Fucini, Michel R. Messora, Valdir G. Garcia, Suely R. M. Bomfim, Edilson Ervolino, Maria J. H. Nagata
Summary: The combination of BMA and LLLT enhances bone healing by improving the expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biochemistry & Molecular Biology
Cheng-Chang Lu, Cheng-Jung Ho, Hsuan-Ti Huang, Sung-Yen Lin, Shih-Hsiang Chou, Pei-Hsi Chou, Mei-Ling Ho, Yin-Chun Tien
Summary: Both bone marrow mononuclear cells (BMMNCs) and bone marrow stromal cells (BMSCs) have comparable effects on cell repopulation and interfacial allograft-bone healing after allograft anterior cruciate ligament reconstruction (ACLR). Intra-articular delivery of BM cells has limited benefits on graft cell repopulation and may cause higher inflammation in the early stage, with fibrocartilage formation in the tendon-bone interface.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Jie Tao, Shunyao Zhu, Xueyuan Liao, Yu Wang, Nazi Zhou, Zhan Li, Haoyuan Wan, Yaping Tang, Sen Yang, Ting Du, Yang Yang, Jinlin Song, Rui Liu
Summary: The integration of 3D bioprinting and stem cells holds great promise for cranial defect reconstruction. A void-forming hydrogel for bone regeneration is developed through the use of digital light processing (DLP)-based bioprinting techniques. The hydrogel promotes the behavior and function of bone marrow stem cells (BMSCs) and enhances their osteogenic differentiation. In vivo assessments demonstrate the potential of the void-forming hydrogel for BMSCs delivery and its ability to significantly promote bone regeneration.
MATERIALS TODAY BIO
(2022)
Article
Emergency Medicine
Nils Muehlenfeld, Philipp Thoenissen, Rene Verboket, Robert Sader, Ingo Marzi, Shahram Ghanaati
Summary: The aim of this study was to analyze the frequency and characteristics of combined facial and peripheral trauma with consecutive hospitalization and treatment. The study found a high frequency of combined facial injuries with orthopedic-traumatolgical injuries and brain damage, predominantly in a young and male cohort. Adequate treatment should be provided by an interdisciplinary team of CMF and OT surgeons specialized in cervical spine trauma surgery to avoid missed diagnoses and keep mortality low.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Surgery
J. Sterz, V. Britz, P. Carstensen, T. Kollewe, S. H. Voss, M. C. Stefanescu, T. Schreckenbach, R. D. Verboket, Miriam Ruesseler
Summary: All 22 participants acknowledged the importance of teaching in clinical practice, but noted that learning goals are not always achieved due to lack of time. Most respondents mentioned feeling unappreciated for teaching, yet found the task to be extremely worthwhile.
Article
Emergency Medicine
Lukas Benedikt Seifert, Tim Mainka, Carlos Herrera-Vizcaino, Rene Verboket, Robert Sader
Summary: This study retrospectively reviewed 1594 patients with midface and orbital fractures treated at Goethe University Hospital in Frankfurt, Germany, between 2007 and 2017. Results showed that most patients were male, with the most common causes of injury being physical assault and falls. The use of 0.15 mm resorbable polydioxanone sheets for orbital floor reconstruction led to significantly fewer post-operative complications.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Emergency Medicine
Jasmina Sterz, Niklas Gutenberger, Maria-Christina Stefanescu, Uwe Zinsser, Lena Bepler, Svea Linssen, Verena Schaefer, Patrick Carstensen, Rene Danilo Verboket, Farzin Adili, Miriam Ruesseler
Summary: This study on emergency medicine training for undergraduate medical students found that simulated patients are rated as more realistic and superior to manikins in terms of gained competence. Students preferred training with simulated patients and scored higher in objective structured clinical examination (OSCE) when trained with simulated patients for 'chest pain' and 'impaired consciousness'.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Environmental Sciences
Cora Rebecca Schindler, Mathias Woschek, Nils Muehlenfeld, Lukas Seifert, Ingo Marzi, Philipp Stoermann, Rene Danilo Verboket
Summary: Demographic change has a major impact on the economic and structural development of the healthcare system. This study uses mTBI as an example diagnosis to illustrate the impact of demographic change and the increasing treatment of geriatric trauma patients on the cost structure of the health care system.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Cell Biology
Sebastian Renelt, Patrizia Schult-Dietrich, Hanna-Mari Baldauf, Stefan Stein, Gerrit Kann, Markus Bickel, Ulrikke Kielland-Kaisen, Halvard Bonig, Rolf Marschalek, Michael A. Rieger, Ursula Dietrich, Ralf Duerr
Summary: This study provides additional evidence for the susceptibility of various types of hematopoietic stem and progenitor cells to HIV-1 infection. The findings suggest that these cells can contribute to the reservoir of HIV-1 in infected individuals, posing a significant obstacle to finding a cure for HIV-1.
Article
Critical Care Medicine
M. Voth, R. Verboket, D. Henrich, I. Marzi
Summary: Delayed diagnosis of abdominal injuries and hemorrhagic shock leads to secondary complications and high late mortality in severely traumatized patients. L-FABP and NGAL are novel biomarkers for detection of abdominal trauma and hemorrhagic shock. L-FABP may be a useful and promising parameter in diagnosis of liver and kidney injuries, NGAL failed to achieve the same.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2023)
Article
Medicine, General & Internal
Cora R. Schindler, Alicia Best, Mathias Woschek, Rene D. Verboket, Ingo Marzi, Katrin Eichler, Philipp Stoermann
Summary: The study aims to evaluate the diagnostic necessity of cranial computed tomography (cCT) to rule out intracranial hemorrhage in elderly patients on permanent anticoagulation. It found that even in asymptomatic elderly patients on anticoagulation, intracranial hemorrhage occurs in a relevant proportion after minor trauma to the head, validating the use of cCT for diagnosis.
FRONTIERS IN MEDICINE
(2023)
Article
Hematology
Peter Bader, Claudia Rossig, Martin Hutter, Francis Ayuketang Ayuk, Claudia D. Baldus, Veit L. Buecklein, Halvard Bonig, Gunnar Cario, Hermann Einsele, Udo Holtick, Christian Koenecke, Shahrzad Bakhtiar, Annette Kuenkele, Roland Meisel, Fabian Mueller, Ingo Mueller, Olaf Penack, Eva Rettinger, Martin G. Sauer, Paul -Gerhardt Schlegel, Jan Soerensen, Arend von Stackelberg, Brigitte Strahm, Julia Hauer, Tobias Feuchtinger, Andrea Jarisch
Summary: This retrospective study investigated the efficacy of Tisa-cel in 81 patients with pB-ALL. The results showed that CAR T cell therapy can effectively alleviate the disease, with a 2-year survival rate of 45.3%. Importantly, patients who relapsed after 6 months post-allo-HSCT had a higher survival rate, which provides valuable information for clinical decision-making.
Editorial Material
Cell & Tissue Engineering
Maximilian Lehmann, Halvard Bonig
Article
Medicine, General & Internal
Meike B. Kejwal, Rene D. Verboket, Katharina Sommer, Fabian Dust, Dominique Thomas, Philipp Stoermann, Johannes Frank, Dirk Henrich, Ingo Marzi, Maren C. Janko
Summary: Radical surgical debridement and systemic antibiotic therapy are essential for treating acute and chronic soft tissue infections. Local antibiotics and/or antibiotic-containing materials are commonly used in addition to these treatments. The spraying technique with fibrin and antibiotics has shown promising results, although there is limited data available for gentamicin. In an animal study, concurrent application of gentamicin and fibrin via spray system yielded significant and prolonged antibiotic concentration in soft tissue wounds, potentially leading to improved local antibiotic therapy.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Nicolas Soehling, Myriam Heilani, Charlotte Fremdling, Alexander Schaible, Katrin Schroeder, Jan C. Brune, Volker Eras, Christoph Nau, Ingo Marzi, Dirk Henrich, Rene D. Verboket
Summary: The classic two-stage masquelet technique is effective for treating large bone defects. Using decellularized dermis membrane (DCD) can save one surgery. It is also possible to use granular demineralized bone matrix (g-DBM) or fibrous demineralized bone matrix (f-DBM) instead of syngeneic cancellous bone (SCB) for defect filling.
Article
Critical Care Medicine
Jan Tilmann Vollrath, Cora Rebecca Schindler, Eva Herrmann, Rene D. Verboket, Dirk Henrich, Ingo Marzi, Philipp Stoermann
Summary: This study evaluated the ability of different serum markers to identify polytraumatized patients at risk of developing pneumonia. The study found that individual serum markers were not helpful in accurately identifying the risk of pneumonia, but a combination of IL-6, IL-10, PTX-3, and sRAGE may be useful for identification.
Article
Biotechnology & Applied Microbiology
Nicolas Soehling, Olivia Von Jan, Maren Janko, Christoph Nau, Ulrike Ritz, Ingo Marzi, Dirk Henrich, Rene D. Verboket
Summary: Bone healing research relies on animal models and the evaluation of different parameters. In this study, reevaluation and correlation analysis of image data showed that histological staining can be used to evaluate biomechanical properties of bone, while osteocalcin determination has no predictive value.
BIOENGINEERING-BASEL
(2023)
Article
Emergency Medicine
Nils Muehlenfeld, Jan Tilmann Vollrath, Jason-Alexander Hoerauf, Oliver Schoeffski, Jasmina Sterz, Julia Riemenschneider, Philipp Stoermann, Ingo Marzi, Rene D. Verboket
Summary: Due to the increasing volume of patients with minor injuries presenting in the emergency department, there is a significant imbalance between medical resources and costs, resulting in a revenue deficit for the hospital. Improvements in the allocation of medical resources and the reimbursement system for patients with minor injuries are urgently needed.