Review
Biochemistry & Molecular Biology
Andras Piffko, Christian Uhl, Peter Vajkoczy, Marcus Czabanka, Thomas Broggini
Summary: EphrinB2-EphB4 signaling plays critical roles in embryogenesis and cancer pathologies. This signaling is involved in various cancer processes, such as migration, angiogenesis, and metastasis. However, the signaling pathways differ depending on the cancer type. This study explores the impact of these signaling pathways in neurooncological diseases, including glioma and brain metastasis, and seeks to understand their role in antiangiogenic therapy resistance, particularly in glioma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Tiancheng Li, Han Wang, Chunxiao Lv, Li Huang, Cheng Zhang, Chenchen Zhou, Shujuan Zou, Peipei Duan
Summary: Intermittent PTH promotes periodontal repair by accelerating the regeneration of root resorption through ephrinB2-EPHB4 forward signaling pathway, as evidenced by in vivo and in vitro experiments.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shilpa Bhatia, Diemmy Nguyen, Laurel B. Darragh, Benjamin Van Court, Jaspreet Sharma, Michael W. Knitz, Miles Piper, Sanjana Bukkapatnam, Jacob Gadwa, Thomas E. Bickett, Shiv Bhuvane, Sophia Corbo, Brian Wu, Yichien Lee, Mayumi Fujita, Molishree Joshi, Lynn E. Heasley, Robert L. Ferris, Olga Rodriguez, Christopher Albanese, Mohit Kapoor, Elena B. Pasquale, Sana D. Karam
Summary: The study reveals that EphB4 acts as tumor suppressor and ephrinB2 acts as tumor promoter in head and neck squamous cell carcinoma. Manipulating these signaling pathways can have an impact on tumor growth and angiogenesis.
NATURE COMMUNICATIONS
(2022)
Letter
Medicine, General & Internal
Benjamin Sommer Thinggaard, Frederik Pedersen, Jakob Grauslund, Lonny Stokholm
Summary: This cohort study examines the use and forecast of intravitreal injections of vascular endothelial growth factor inhibitor in Denmark from 2007 through 2022.
Review
Biochemistry & Molecular Biology
Genevieve A. Secker, Natasha L. Harvey
Summary: The importance of lymphatic vessels in human diseases, the initiation of lymphatic vascular development during embryogenesis, and the role of VEGF/VEGFR signaling in driving the construction of lymphatic vessels were discussed in this review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Carlos Escudero, Jesenia Acurio, Eduardo Lopez, Andres Rodriguez, Antonia Benavente, Evelyn Lara, Steven J. Korzeniewski
Summary: Circulating VEGF levels were found to be elevated 24-48 hours after acute ischemic stroke and were associated with prognosis of moderate to severe disability at 6 months. Combining VEGF with interleukin 6 and C-reactive protein significantly improved predictive performance compared to using VEGF alone.
EUROPEAN JOURNAL OF NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fuensanta Gomez-Bernal, Yolanda Fernandez-Cladera, Juan Carlos Quevedo-Abeledo, Maria Garcia-Gonzalez, Agustin F. Gonzalez-Rivero, Antonia De Vera-Gonzalez, Candelaria Martin-Gonzalez, Miguel A. Gonzalez-Gay, Ivan Ferraz-Amaro
Summary: This study analyzed the relationship between VEGF and sVEGFR with SLE disease-related features and found that serum levels of VEGF and sVEGFR in SLE patients were associated with disease damage, with musculoskeletal manifestations being the most commonly associated feature.
Article
Pharmacology & Pharmacy
Jun-Hei Chang, Chen-Chuan Cheng, Yen-Yu Lu, Cheng-Chih Chung, Yung-Hsin Yeh, Yao-Chang Chen, Satoshi Higa, Shih-Ann Chen, Yi-Jen Chen
Summary: VEGF reduces PV arrhythmogenesis by modulating sodium/calcium homeostasis through VEGFR-1/NOS signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jie Zhou, Yanlin Zhu, Dongqing Ai, Mengjiao Zhou, Han Li, Yiru Fu, Jinlin Song
Summary: Low-intensity pulsed ultrasound (LIPUS) can improve orthodontic tooth movement and alveolar bone remodeling by promoting the interaction between osteoblasts and osteoclasts, thus enhancing the effectiveness of orthodontic treatment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Xiao-yu Dai, Li-jun Ren, Lang Yan, Ji-qian-zhu Zhang, Yi-fan Dong, Tao-lin Qing, Wen-jing Shi, Jin-feng Li, Fang-yuan Gao, Xiao-fang Zhang, Yi-jun Tian, Yu-ping Zhu, Jiang-bo Zhu, Ji-kuai Chen
Summary: This study demonstrated that MWCNTs can affect endothelial cell activity, disrupt tube formation and cell migration ability, subsequently affecting angiogenesis. Furthermore, the VEGF-Akt-eNOS axis plays a crucial role in MWCNT-induced endothelial cell injury.
Article
Hematology
Kathryn N. Phoenix, Zhichao Yue, Lixia Yue, Chunxia G. Cronin, Bruce T. Liang, Luke H. Hoeppner, Kevin P. Claffey
Summary: This study identifies PLC beta 2 as a key regulator of vascular permeability induced by VEGF, functioning through regulation of calcium flux and phosphatidylinositol 4,5-bisphosphate levels. Targeting PLC beta 2 could provide a novel approach to regulate vascular permeability in patients.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Prashant Jain, Chethan D. Shanthamurthy, Shani Leviatan Ben-Arye, Sharon Yehuda, Sharvani S. Nandikol, Hirekodathakallu Thulasiram, Vered Padler-Karavani, Raghavendra Kikkeri
Summary: A potential heparan sulfate (HS) ligand was discovered to target several growth factors using specific HS tetrasaccharide ligands. One tetrasaccharide ligand showed strong binding with the VEGF(165) growth factor, and in vitro experiments demonstrated the therapeutic potential of these synthetic HS ligands by inhibiting vascular endothelial cell proliferation, migration, and angiogenesis in the presence of VEGF(165) and the specific tetrasaccharides.
CHEMICAL COMMUNICATIONS
(2021)
Article
Clinical Neurology
Il-doo Kim, John W. Cave, Sunghee Cho
Summary: Obese mice with acute stroke showed increased mortality, infarct volume, swelling, and blood-brain barrier disruption, along with elevated VEGF-A and VEGFR2 expression. Aflibercept could reduce brain edema and vascular permeability, improving stroke outcomes in obese subjects.
Article
Multidisciplinary Sciences
John-Michael Arpino, Hao Yin, Emma K. Prescott, Sabrina C. R. Staples, Zengxuan Nong, Fuyan Li, Jacqueline Chevalier, Brittany Balint, Caroline O'Neil, Rokhsana Mortuza, Stephanie Milkovich, Jason J. Lee, Daniel Lorusso, Martin Sandig, Douglas W. Hamilton, David W. Holdsworth, Tamie L. Poepping, Christopher G. Ellis, J. Geoffrey Pickering
Summary: Contrary to prevailing beliefs, angiogenesis in ischemic muscle injury is driven by the formation of wide-caliber conduits that rapidly transform into a hierarchical neocirculation. Inhibiting VEGFR2 accelerates this process, and endothelial cells in low-flow lumens display enhanced VEGFR2 mechanosignals.
Article
Chemistry, Multidisciplinary
Yang Liu, Linyu Long, Fanjun Zhang, Xuefeng Hu, Jieyu Zhang, Cheng Hu, Yunbing Wang, Jianguo Xu
Summary: The study introduced a GelMA microneedle platform for sustained and controlled local delivery of AAV-VEGF, which achieved homogeneous distribution and high transfection efficiency in ischemic brains. Results showed that MNs loaded with AAV-VEGF increased VEGF expression in the brain cortex and enhanced functional angiogenesis and neurogenesis.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Cell Biology
Jing Zhang, Jonathan Muri, Gillian Fitzgerald, Tatiane Gorski, Roberto Gianni-Barrera, Evi Masschelein, Gommaar D'Hulst, Paola Gilardoni, Guillermo Turiel, Zheng Fan, TongTong Wang, Melanie Planque, Peter Carmeliet, Luc Pellerin, Christian Wolfrum, Sarah-Maria Fendt, Andrea Banfi, Christian Stockmann, Ines Soro-Arnaiz, Manfred Kopf, Katrien De Bock
Article
Biotechnology & Applied Microbiology
Rene' D. Largo, Maximilian G. Burger, Oliver Harschnitz, Conny F. Waschkies, Andrea Grosso, Celeste Scotti, Alexandre Kaempfen, Sinan Gueven, Gernot Jundt, Arnaud Scherberich, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Cell & Tissue Engineering
Ludovic Melly, Andrea Grosso, Claudia Stanciu Pop, Chu Yu-Hsuan, Marie-Cecile Nollevaux, Christian Schachtrup, Anna Marsano, Nunzia Di Maggio, Benoit Rondelet, Andrea Banfi
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2020)
Article
Cell Biology
Cinzia Donato, Leo Kunz, Francesc Castro-Giner, Aino Paasinen-Sohns, Karin Strittmatter, Barbara Maria Szczerba, Ramona Scherrer, Nunzia Di Maggio, Wolf Heusermann, Oliver Biehlmaier, Christian Beisel, Marcus Vetter, Christoph Rochlitz, Walter Paul Weber, Andrea Banfi, Timm Schroeder, Nicola Aceto
Review
Engineering, Biomedical
Raquel C. Goncalves, Andrea Banfi, Mariana B. Oliveira, Joao F. Mano
Summary: Maintaining a healthy vascular system is crucial for the proper function of all human organs. Atherosclerosis remains the leading cause of mortality worldwide, driving the need for new vascular substitutes and stable microvasculature formation. Research in bioinspired strategies is growing to ensure vascular function in all dimensions.
Article
Medicine, Research & Experimental
Federica Zilli, Pedro Marques Ramos, Priska Auf Der Maur, Charly Jehanno, Atul Sethi, Marie-May Coissieux, Tobias Eichlisberger, Loic Sauteur, Adelin Rouchon, Laura Bonapace, Joana Pinto Couto, Roland Rad, Michael Rugaard Jensen, Andrea Banfi, Michael B. Stadler, Mohamed Bentires-Alj
Summary: NFIB is a transcription factor that plays a crucial role in promoting primary mammary tumor growth and lung metastatic colonization by increasing the expression of ERO1A. Its high expression is associated with poor prognosis in basal-like breast cancer patients.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Alessandro Certelli, Paolo Valente, Andrea Uccelli, Andrea Grosso, Nunzia Di Maggio, Rosalinda D'Amico, Priscilla S. Briquez, Jeffrey A. Hubbell, Thomas Wolff, Lorenz Gurke, Edin Mujagic, Roberto Gianni-Barrera, Andrea Banfi
Summary: In diabetic mouse skin, controlled co-delivery of TG-VEGF and TG-PDGF-BB recombinant proteins effectively induces angiogenesis and arteriogenesis, with similar efficacy to normal littermate control mice at therapeutically relevant doses. This approach also prevents abnormal vascular enlargement caused by high TG-VEGF levels and promotes effective arteriogenesis in the dermal layer.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Cell Biology
Ludovic Melly, Andrea Banfi
Summary: Therapeutic angiogenesis aims to promote blood vessel growth to restore perfusion in ischemic tissues or aid tissue regeneration. Vascular endothelial growth factor (VEGF) plays a crucial role in this process. Fibrin, a provisional matrix deposited after injury, can be used as a scaffold material to promote therapeutic vascularization. However, fibrin may also lead to undesirable outcomes such as fibrosis and scar formation, which hinder physiological tissue regeneration. Therefore, understanding the balance between the pro- and anti-regenerative functions of fibrin is key to fully exploiting its therapeutic potential.
CELL AND TISSUE RESEARCH
(2022)
Review
Biochemical Research Methods
Nunzia Di Maggio, Andrea Banfi
Summary: Recent studies have shown that blood vessels have a regulatory role in bone development and repair, beyond their traditional function of supplying nutrients and oxygen. Type H endothelium, a specialized type of endothelial cells, plays a crucial role in regulating the proliferation and differentiation of osteoblasts and osteoclasts through the secretion of angiocrine signals, facilitating communication between different cell populations in the bone microenvironment. Targeting Type H vessels has become a focus of translational research for bone repair, but there are still unanswered questions about their presence and potential stimulation through pharmacological treatments in non-stereotyped tissues.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Maximilian G. Burger, Andrea Grosso, Priscilla S. Briquez, Gordian M. E. Born, Alexander Lunger, Flavio Schrenk, Atanas Todorov, Veronica Sacchi, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: In regenerative medicine, rapid vascularization of clinical-size bone grafts is a challenge that has not been solved. This study investigates the distribution of VEGF protein on matrices to achieve efficient coupling of angiogenesis and bone formation, providing a clinically applicable strategy for engineering vascularized bone.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
Encheng Ji, Lieke Leijsten, Janneke Witte-Bouma, Adelin Rouchon, Nunzia Di Maggio, Andrea Banfi, Gerjo J. V. M. van Osch, Eric Farrell, Andrea Lolli
Summary: Tissue engineering bone via endochondral ossification requires the generation of a mineralized cartilage template. Our study investigated the effects of cartilage mineralization on its pro-angiogenic potential. We found that mineralized cartilage showed decreased production of pro-angiogenic factors and reduced ability to stimulate endothelial cell migration, proliferation, and tube formation. This stage-dependent pro-angiogenic potential should be considered in bone tissue engineering strategies.
Article
Cell & Tissue Engineering
Andrea Grosso, Alexander Lunger, Maximilian G. Burger, Priscilla S. Briquez, Francesca Mai, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: Vascular endothelial growth factor-A (VEGF) has a physiological role in regulating both angiogenesis and osteogenesis, but its application in bone tissue engineering has produced conflicting outcomes. This study investigates the impact of VEGF dose on the coordination between angiogenesis and osteogenesis. The findings suggest that a specific range of VEGF dose is required to stimulate both vascular invasion and osteogenic commitment, resulting in improved bone formation. The study also reveals that VEGF dose regulates Notch1 activation and induces a pro-osteogenic endothelial phenotype. Fine-tuning of VEGF dose is crucial for achieving the physiological coupling of accelerated vascular invasion and enhanced bone formation.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Cell & Tissue Engineering
Rosalinda D'Amico, Camilla Malucelli, Andrea Uccelli, Andrea Grosso, Nunzia Di Maggio, Priscilla S. Briquez, Jeffrey A. Hubbell, Thomas Wolff, Lorenz Gurke, Edin Mujagic, Roberto Gianni-Barrera, Andrea Banfi
Summary: Chronic wounds in type-2 diabetic patients often have severe local skin ischemia despite normal blood flow. By using fibrin hydrogels decorated with engineered growth factors, wound closure and microvascular growth can be promoted.
JOURNAL OF TISSUE ENGINEERING
(2022)