Article
Biochemistry & Molecular Biology
Anja C. Nagel, Dominik Mueller, Mirjam Zimmermann, Anette Preiss
Summary: The membrane-bound Notch regulator (Mnr) supports Notch signaling by promoting the cleavage of the Notch receptor, which in turn enhances Notch receptor activation. Overexpression of Mnr stimulates γ-secretase activity, while knockdown of Mnr inhibits this activity, demonstrating Mnr as a positive regulator of Notch signaling.
Article
Multidisciplinary Sciences
Stamatis Papathanasiou, Styliani Markoulaki, Logan J. Blaine, Mitchell L. Leibowitz, Cheng-Zhong Zhang, Rudolf Jaenisch, David Pellman
Summary: The study tracks aberrations in karyotypes during embryonic development post-Cas9 editing, revealing unwanted on-target complications including major chromosome structural alterations like whole chromosome loss. Micronuclei and chromosome bridges are observed as consequences of mitosis errors, contributing to these common karyotype aberrations after Cas9 treatment in embryos.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Iowis Zhu, Raymond Liu, Julie M. Garcia, Axel Hyrenius-Wittsten, Dan Piraner, Josef Alavi, Divya Israni, Bin Liu, Ahmad S. Khalil, Kole T. Roybal
Summary: Synthetic biology has the potential to meet clinical requirements by precisely controlling cell function. By systematically engineering key domains involved in regulated intramembrane proteolysis, researchers have developed synthetic intramembrane proteolysis receptors (SNIPRs) that have tunable sensing and transcriptional response abilities. Engineering human primary T cells with this receptor platform demonstrates its therapeutic potential.
Article
Biochemistry & Molecular Biology
Colin H. Lipper, Khal-Hentz Gabriel, Tom C. M. Seegar, Katharina L. Durr, Michael G. Tomlinson, Stephen C. Blacklow
Summary: Tspan15, a member of the TspanC8 subfamily, forms a complex that regulates protein cleavage by binding to ADAM10. The structure of Tspan15's large extracellular loop shows a unique core helical framework supporting a variable region that differs from other tetraspanin LEL structures.
Article
Multidisciplinary Sciences
Nicole Scholz, Anne-Kristin Dahse, Marguerite Kemkemer, Anne Bormann, Genevieve M. Auger, Fernando Vieira Contreras, Lucia F. Ernst, Hauke Staake, Marek B. Koerner, Max Buhlan, Amelie Meyer-Moelck, Yin Kwan Chung, Beatriz Blanco-Redondo, Franziska Klose, Mohamed Ali Jarboui, Dmitrij Ljaschenko, Marina Bigl, Tobias Langenhan
Summary: This study investigates the autoproteolytic processing of adhesion G-protein-coupled receptors (aGPCRs). The researchers introduce a genetically encoded sensor system to detect the dissociation events of aGPCR heterodimers and find that mechanical force can stimulate the release of aGPCR fragments. The dissociation of aGPCRs is controlled by ligand expression and mechanical force, and understanding their physiological roles can have important implications for various diseases.
Article
Multidisciplinary Sciences
Tim J. W. Harmsen, Colin E. J. Pritchard, Joey Riepsaame, Henri J. van de Vrugt, Ivo J. Huijbers, Hein te Riele
Summary: The presence of both trimmed guideRNA and a hideRNA matching the successfully mutated sequence can significantly enhance the recovery of precise single base-pair substitution events. This method allows for improved accuracy in gene editing experiments.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
G. Cullot, J. Boutin, S. Fayet, F. Prat, J. Rosier, D. Cappellen, I. Lamrissi, P. Pennamen, J. Bouron, S. Amintas, C. Thibault, I. Moranvillier, E. Laharanne, J. P. Merlio, V. Guyonnet-Duperat, J. M. Blouin, E. Richard, S. Dabernat, F. Moreau-Gaudry, A. Bedel
Summary: The authors developed a Fluorescence-Assisted Megabase-scale Rearrangements Detection (FAMReD) system to detect rare large loss of heterozygosity. They demonstrated that ON-target genotoxicity can be prevented by p53 and cell cycle arrest. Comprehensive analysis of gene editing products using this system revealed complex outcomes and highlighted the underestimation of ON-target genotoxicity.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Joanna Kalafut, Arkadiusz Czerwonka, Karolina Czapla, Alicja Przybyszewska-Podstawka, Justyna Magdalena Hermanowicz, Adolfo Rivero-Muller, Lidia Borkiewicz
Summary: Notch1 signaling plays a diverse role in tissue development and homeostasis. The relationship between NOTCH1 expression and the development of health disorders, especially cancers, is intensively studied. Control of Notch1 signaling involves not only transcriptional regulation but also various post-translational events, including ligand-dependent mechanical activation and interaction with other signaling molecules, such as long non-coding RNAs (lncRNAs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Santanu Jana, Vikram A. Sarpe, Suvarn S. Kulkarni
Summary: In this study, a total synthesis strategy for the structurally unique fungal glycolipid fusaroside was reported, with a proposed revision in its structure, particularly regarding the position of olefins in the lipid chain.
Article
Materials Science, Multidisciplinary
Yinxia Zhang, Matthias Bartosik, Steffen Brinckmann, Subin Lee, Christoph Kirchlechner
Summary: Focused ion beam (FIB) milling is commonly used for micromechanical testing, but it can introduce artifacts that affect the obtained mechanical properties. A bridge notch geometry was proposed as a strategy to reduce FIB-induced artifacts over a decade ago, but its experimental observation and quantification have not been realized until now. This study presents the first experimental observation of bridge notch failure and crack arrest, providing more accurate fracture toughness values.
MATERIALS & DESIGN
(2023)
Review
Cell Biology
Frederick Allen, Ivan Maillard
Summary: The Notch signaling pathway has been investigated as a therapeutic target for cancers and immune/inflammatory disorders, orchestrating cell fate decisions and regulating immune responses through cell-to-cell contacts. It has shown potential in inhibiting tumor growth and preventing/reversing inflammatory disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Organic
Jianyou Zhao, Tong Shen, Zhihui Sun, Nengyong Wang, Le Yang, Jintao Wu, Huichao You, Zhong-Quan Liu
Summary: The site-specific oxidation of (sp(3))C-C(sp(3)) and (sp(3))C-H bonds in aryl alkanes by NaNO2/HCl is chemical-oxidant-free and transition-metal-free, using water as the solvent under mild conditions. This makes it valuable and attractive to synthetic organic chemistry.
Article
Chemistry, Organic
Jie Zhu, Yun Ye, Yifei Yan, Jinghui Sun, Yinhua Huang
Summary: An unprecedented geminal olefinic dichalcogenation of alkenyl sulfonium salts with dichalcogenides is reported, providing various trisubstituted 1,1-dichalcogenalkenes in a highly selective manner. The key process involves the formation of two geminal olefinic C-Y bonds via sequential C-Y cross-coupling and C-H chalcogenation. Mechanistic rationale is further supported by control experiments and density functional theory calculations.
Article
Chemistry, Organic
Ai-Chun Chiang, Yi-Hung Liu, Shie-Ming Peng, Shiuh-Tzung Liu
Summary: The study revealed that in the presence of Pd catalyst and DBU, (Z)-pent-2-en-4-yn-1-yl alkanoates can undergo acyl migration to yield high yields of 2-(alkanoylmethyl)furan derivatives, followed by oxidative decarbonylation with O2 to form 2-acylfurans. The mechanistic pathway involving intramolecular cyclization and acyl group shift was discussed.
Article
Biochemistry & Molecular Biology
Bin Li, Liang Wang, Xinwei Qi, Yumei Liu, Jiajun Li, Jie Lv, Xuan Zhou, Xuanlin Cai, Jiaoyu Shan, Xiumin Ma
Summary: In this study, the changes in NOTCH signaling pathway, as well as the fibrotic and inflammatory response of the liver after E. multilocularis infection, were analyzed using liver tissue samples from AE patients and an infected mouse model. It was found that blocking NOTCH signaling pathway exacerbates hepatic fibrosis levels and alters the polarization and origin of hepatic macrophages, suggesting the involvement of NOTCH3/DLL3 pathway in regulating macrophage polarization and fibrosis caused by AE.
Article
Cell Biology
Maria J. Gomez-Lamarca, Julia Falo-Sanjuan, Robert Stojnic, Sohaib Abdul Rehman, Leila Muresan, Matthew L. Jones, Zoe Pillidge, Gustavo Cerda-Moya, Zhenyu Yuan, Sarah Baloul, Phillippe Valenti, Kerstin Bystricky, Francois Payre, Kevin O'Holleran, Rhett Kovall, Sarah J. Bray
DEVELOPMENTAL CELL
(2018)
Article
Biology
Hadi Boukhatmi, Sarah Bray
Article
Biochemistry & Molecular Biology
Zoe Pillidge, Sarah J. Bray
Article
Cell Biology
Julia Falo-Sanjuan, Nicholas C. Lammers, Hernan G. Garcia, Sarah J. Bray
DEVELOPMENTAL CELL
(2019)
Review
Cell Biology
Julia Falo-Sanjuan, Sarah J. Bray
DEVELOPMENT GROWTH & DIFFERENTIATION
(2020)
Article
Multidisciplinary Sciences
Yildiz Koca, Benjamin E. Housden, William J. Gault, Sarah J. Bray, Marek Mlodzik
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Hadi Boukhatmi, Torcato Martins, Zoe Pillidge, Tsveta Kamenova, Sarah Bray
Article
Developmental Biology
Shiyun Feng, Evanthia Zacharioudaki, Kat Millen, Sarah J. Bray
NEURAL DEVELOPMENT
(2020)
Article
Cell Biology
Andrea Valencia-Exposito, M. Jesus Gomez-Lamarca, Thomas J. Widmann, Maria D. Martin-Bermudo
Summary: Adhesion to the extracellular matrix (ECM) is crucial for epithelial cell survival, mediated by the integrin family. This study used Drosophila wing imaginal disc epithelium to explore the importance of integrins in epithelial tissue morphogenesis, finding that reduced integrin expression led to cell death and extrusion. The JNK pathway and EGFR pathway were identified as regulators in this process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Evanthia Zacharioudaki, Julia Falo Sanjuan, Sarah Bray
Article
Developmental Biology
Liqun Yao, Shenqiu Wang, Jakub O. Westholm, Qi Dai, Ryo Matsuda, Chie Hosono, Sarah Bray, Eric C. Lai, Christos Samakovlis
Article
Cell Biology
Ana Terriente-Felix, Lidia Perez, Sarah J. Bray, Angel R. Nebreda, Marco Milan
DISEASE MODELS & MECHANISMS
(2017)