Journal
FOOD CHEMISTRY
Volume 186, Issue -, Pages 133-138Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.02.011
Keywords
Heracleum mantegazzianum; High-performance counter-current chromatography; Furanocoumarins; Antimicrobial activity
Funding
- grant for Young Scientists MNmb47 (Poland)
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An efficient strategy, based on bioassay-guided fractionation, high-performance liquid chromatography (HPLC), and high-performance counter-current chromatography (HPCCC), was established to purify and evaluate the bioactive compounds from the dichloromethane extract of the fruits of Heracleum mantegazzianum Sommier & Levier (Apiaceae). The quaternary solvent system n-heptane-ethyl acetate-methanol-water (6:5:6:5 v/v) was used in the reversed phase mode. Using this method, in a single run, seven fractions were isolated, among them three were the pure furanocoumarins: pimpinellin, imperatorin, and phellopterin. In order to purify xanthotoxin a more polar system (1:1:1:1 v/v) was further applied. The antimicrobial activity of extract, chromatographic fractions, and single compounds were in the range of MIC = 0.03-1 mg mL(-1). Xanthotoxin may have priority as a compound of further interest based on its antimicrobial activity. For the first time, an extensive antimicrobial study was performed for pimpinellin and phellopterin. (C) 2015 Elsevier Ltd. All rights reserved.
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