Journal
FOOD CHEMISTRY
Volume 176, Issue -, Pages 254-262Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.12.073
Keywords
Soybean; Isoflavones; High performance liquid chromatography; Time-of-flight mass spectrometry
Funding
- Korean Ministry for Food, Agriculture, Forestry and Fisheries through IPET
Ask authors/readers for more resources
A high resolution LC-ESI-TOF-MS analytical method was established for the rapid isolation, identification, and quantification of 12 isoflavones in soybean and soybean products. Individual isoflavones were identified on the basis of the accurate mass data of their respective protonated mass ions, Na or K adduct ions, fragment ions, and isotope ion patterns. The protonated mass ions of isoflavones were extracted for their quantification in soybean products. Twelve different isoflavones in the soybean products were fully separated, identified and quantified within 12 min separation time. The established LC-TOF/MS was an effective analytical method for the simultaneous characterization and quantification of isoflavones with exceptionally short analytical time, high selectivity, a high linearity (r(2) > 0.992) in a wide range, low LOD and LOQ high precision, inter-and intra-day repeatability, and no significant matrix effect. Furthermore, it requires simple sample preparation procedure (solvent extraction, dilution, and syringe filtration). (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available