Journal
FOOD CHEMISTRY
Volume 175, Issue -, Pages 494-499Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.12.005
Keywords
Quercetin; Apigenin; Platelet aggregation; P-selectin expression; 2-Phenyl-4H-pyrido[1,2-a]pyrimidin-4-ones; 2,3-Diphenyl-4H-pyrido[1,2-a]pyrimidin-4-ones
Funding
- Fondazione Pisa [153/09]
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We investigated the ability of quercetin and apigenin to modulate platelet activation and aggregation, and compared the observed efficacy with that displayed by their synthetic analogues 2-phenyl-4H-pyrido[1,2-a]pyrimidin-4-ones, 1-4, and 2,3-dipheny1-4H-pyrido[1,2-a]pyrimidin-4-ones, 5-7. Platelet aggregation was explored through a spectrophotometric assay on platelet-rich plasma (PRP) treated with the thromboxane A(2) mimetic U46619, collagen and thrombin in presence/absence of various bioisosteres of flavonoids (12.5-25-50-100 mu M). The platelet density, (mean platelet component, MPC), was measured by the Advia 120 Hematology System as a marker surrogate of platelet activation. The induced P-selectin expression, which reflects platelet degranulation/activation, was quantified by flow cytometry on PRP. Our synthetic compounds modulated significantly both platelet activation and aggregation, thus turning out to be more effective than the analogues quercetin and apigenin when tested at a concentration fully consistent with their use in vivo. Accordingly, they might be used as food supplements to increase the efficacy of natural flavonoids. (C) 2014 Elsevier Ltd. All rights reserved.
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