Journal
FOOD CHEMISTRY
Volume 172, Issue -, Pages 596-602Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.09.101
Keywords
Olive oil; DNA extraction; SSR analysis; Cultivar authentication; Traceability
Funding
- P. R. U. S. S. T. C A L I D O N E'' - Programma di Riqualificazione Urbana e. Sviluppo Sostenibile del Territorio
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Extra virgin olive oil production has a worldwide economic impact. The use of this brand, however, is of great concern to Institutions and private industries because of the increasing number of fraud and adulteration attempts to the market products. Here, we present a novel, reliable and not expensive method for extracting the DNA from commercial virgin and extra virgin olive oils. The DNA is stable overtime and amenable for molecular analyses; in fact, by carrying out simple sequence repeats (SSRs) markers analysis, we characterise the genetic profile of monovarietal olive oils. By comparing the oil-derived pattern with that of the corresponding tree, we can unambiguously identify four cultivars from Samnium, a region of Southern Italy, and distinguish them from reference and more widely used varieties. Through a parentage statistical analysis, we also identify the putative pollinators, establishing an unprecedented and powerful tool for olive oil traceability. (C) 2014 Elsevier Ltd. All rights reserved.
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