Article
Integrative & Complementary Medicine
Jianghui Hu, Jimin Ni, Junping Zheng, Yanlei Guo, Yong Yang, Cheng Ye, Xiongjie Sun, Hui Xia, Yanju Liu, Hongtao Liu
Summary: This study found that Tripterygium hypoglaucum extract can protect against rheumatoid arthritis by manipulating the gut microbiota and regulating the TLR4/ MyD88/ MAPK signaling pathway. Additionally, treatment with Tripterygium hypoglaucum extract failed to improve arthritis symptoms and signaling pathways in pseudo germ-free mice, further validating the importance of the gut microbiota.
CHINESE JOURNAL OF NATURAL MEDICINES
(2023)
Review
Pharmacology & Pharmacy
Junqi Zhao, Fangling Zhang, Xiaolin Xiao, Zhao Wu, Qichao Hu, Yinxiao Jiang, Wenwen Zhang, Shizhang Wei, Xiao Ma, Xiaomei Zhang
Summary: Tripterygium hypoglaucum (Levl.) Hutch (THH) is considered a significant herb in traditional Chinese medicine, known for its notable efficacy and potential toxicity. Recent studies have revealed its diverse secondary metabolites which may contribute to various pharmacological effects, including immunosuppression, anti-inflammatory, anti-tumor properties. In addition, THH has also shown promising effects in obesity, insulin resistance, fertility, and antiviral activities. Pharmacokinetic studies have indicated rapid elimination of the main component triptolide, while toxicity mainly affects organs such as the liver, kidneys, and reproductive system. Efforts to combine medications to balance efficacy and reduce toxicity, along with advancements in technology like structure optimization, may hold potential for clinical applications of THH.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Integrative & Complementary Medicine
Chen Xiao-Chao, Lu Yun, Liu Yuan, Zhou Jia-Wei, Zhang Yi-Feng, Gao Hai-Yun, Li Dan, Gao Wei
Summary: Tripterygium hypoglaucum (Levl.) Hutch, a traditional Chinese medicinal herb, contains celastrol, a potential anti-cancer and anti-obesity drug. The lack of sustainable sources of celastrol and its derivatives necessitates the development of novel sources through synthetic biology. A cytochrome P450 ThCYP712K1 from Tripterygium hypoglaucum was found to catalyze the oxidation of friedelin into polpuonic acid, a vital step in celastrol biosynthesis.
CHINESE JOURNAL OF NATURAL MEDICINES
(2022)
Article
Plant Sciences
Xing-Long Chen, Yi-Juan Geng, Fei Li, Wei-Yan Hu, Rong-Ping Zhang
Summary: This study found that terpenoids from Tripterygium hypoglaucum can inhibit pancreatic cancer cells and increase Bax protein expression. These findings provide candidate molecules for the development of anti-pancreatic cancer drugs.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Plant Sciences
Chengyan Long, Yang Yang, Yunhong Wang, Xiaomei Zhang, Li Zhang, Sixing Huang, Dajian Yang, Xingfang Qiao, Yong Yang, Yanlei Guo
Summary: The study demonstrated that THH extract showed beneficial effects on rheumatoid arthritis in a rat model, improving body weight, reducing joint damage, and modulating immune responses. Metabolomics analysis identified important metabolic pathways affected by THHE administration, suggesting potential targets for therapy development.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Junping Zheng, Jianghui Hu, Yong Yang, Lei Xiong, Huabing Yang, Zhigang Zhang, Nan Jiang, Hongtao Liu
Summary: This study reveals the critical role of bile acid-mediated gut-joint axis and inflammasome in the mechanism of Tripterygium hypoglaucum's alleviation of rheumatoid arthritis. The study shows that Tripterygium hypoglaucum treatment can suppress NLRP3-mediated inflammasome and remodel bile acid profiles, resulting in reduced joint swelling and disease scores in rheumatoid arthritis mice.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Yadan Wang, Jiangong Yan, Zhongmou Zhang, Minghui Chen, Xianfu Wu, Shuangcheng Ma
Summary: In this study, ten new sesquiterpene pyridine alkaloids (SPAs) were isolated from Tripterygium wilfordii Hook. f., a traditional Chinese medicine used to treat autoimmune diseases. Four of these compounds were found to exhibit potent immunosuppressive activity, indicating the potential of T. wilfordii as an immunomodulatory agent.
Article
Integrative & Complementary Medicine
Dan Zhang, Yizhu Dong, Jintao Lv, Bing Zhang, Xiaomeng Zhang, Zhijian Lin
Summary: This study elucidated the synergistic interaction and mechanism of efficacy and toxicity for Tripterygium hypoglaucum Hutch (THH) in the treatment of rheumatoid arthritis and systemic lupus erythematosus. The research also revealed the mechanism of adverse reactions induced by THH and the superior binding activities between active ingredients and crucial targets through molecular docking simulation.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2021)
Article
Plant Sciences
Shunan Chen, Yunfei Wang, Yi Li, Xuewen Zhang, Jie Wu, Takayuki Ohgushi
Summary: This study examines the fluorescence of toxic plants (Tripterygium hypoglaucum), the behavior of honeybees (Apis cerana) during flower visits, and the effects of honeybee pollination on the reproduction of toxic plants. The research findings demonstrate that honeybees play a significant role in facilitating the reproduction of toxic plants, and there is a strong correlation between the number of bee visits and the spatio-temporal variation in the number of flowers.
Article
Plant Sciences
Manyun Dai, Wan Peng, Ting Zhang, Qi Zhao, Xiaofang Ma, Yan Cheng, Chunyan Wang, Fei Li
Summary: This study revealed that Tripterygium wilfordii tablets (TWT) induced liver injury through disrupting the PPAR alpha-IL6-STAT3 axis and causing an accumulation of medium- and long-chain carnitines metabolism. Activation of PPAR alpha by fenofibrate could reverse the liver injury induced by TWT and TP/Cel, but its protective role was attenuated in Ppara-null mice. The difference in toxicity between TWT and Tripterygium glycosides tablets (TGT) was attributed to the different ratio of triptolide (TP) and celastrol (Cel) in the tablet. Hepatotoxicity induced by TP and Cel inhibited PPAR alpha and upregulated the IL6-STAT3 axis, which could be alleviated by PPAR alpha activation.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sofia Tsouka, Mojgan Masoodi
Summary: Pathway analysis is commonly used for functional interpretation of metabolomics data, but lacks standardization and understanding of its impact on functional outcome. In this study, we investigated the consideration of non-human native enzymatic reactions and interconnectivity of pathways in pathway analysis. Exclusion of non-human reactions resulted in loss of information, while considering pathway connectivity provided better emphasis on certain metabolites but occasionally overemphasized central compounds. We proposed a penalization scheme to mitigate the effect of such compounds. We also performed over-representation analysis to compare and assess different methodologies. Our findings raise awareness on the capabilities and shortcomings of current pathway analysis practices in metabolomics.
Article
Biochemistry & Molecular Biology
Suzanne Paley, Richard Billington, James Herson, Markus Krummenacker, Peter D. Karp
Summary: Metabolomics, synthetic biology, and microbiome research require information on organism-scale metabolic networks. Software tools have been developed to generate and analyze comprehensive metabolic-network diagrams, aiding researchers in understanding, analyzing, and exploiting metabolic reconstructions. These tools also enable comparisons of metabolic networks and interpretation of high-throughput datasets.
Article
Agriculture, Multidisciplinary
Hua Yang, Ying Xie, Xiaomin Li, Dianhui Wu, Guolin Cai, Jian Lu
Summary: Untargeted metabolomics analysis using HILIC LC-MS identified 22 key differential metabolites associated with browning of Dangshan pear wine, including glucose, phenylalanine, aloin, etc. Maillard reaction and oxidation were critical pathways in the browning process.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Guangchao Cao, Ruonan Ning, Jie Zhao, Jiaying Yao, Linyue Chang, Hongyan Mu, Rujun Zhang, Zhenhua Chen, Fengbei Gong, Shijun He, Min Jiang, Weimin Zhao
Summary: Extensive phytochemical investigation of Tripterygium wilfordii seeds led to the identification of 54 polyesterified dihydro-beta-agarofuran-type sesquiterpenoids, including 27 previously undescribed ones. Some of these compounds showed significant inhibition against osteoclastogenesis and lymphocyte proliferation.
BIOORGANIC CHEMISTRY
(2023)
Article
Agricultural Engineering
Manish Paul, Niteesh Kumar Pandey, Ayan Banerjee, Gireesh Kumar Shroti, Preeti Tomer, Rajesh Kumar Gazara, Hrudayanath Thatoi, Thallada Bhaskar, Saugata Hazra, Debashish Ghosh
Summary: This review focuses on the research progress of lignin-degrading microorganisms and enzymes associated with ligninolytic activity, which play a crucial role in the breakdown of lignocellulosic biomass. Analyzing multi-omics data highlights the potential value of fungal-produced enzymes in lignocellulosic material degradation, giving them an advantage in their ecological niches.
BIORESOURCE TECHNOLOGY
(2023)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)