Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 26, Issue 5, Pages 729-737Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2015.1018875
Keywords
strip test; chlorothalonil; rapid detection; colloidal gold; immunoassay
Funding
- Surveillance of Safety and Studies on the Detection Techniques of Animal products from the Supporting Program of the 13th Five-year Plan [2014BAD13B05]
- Sichuan Agricultural University [00770107]
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An immunochromatographic lateral flow strip test using the competitive format was developed for the determination of chlorothalonil (CTN) residues in cucumber. The limit of detection was less than 100 ng/mL by visual assessment and was found to be 91.78 +/- 0.17 ng/mL for quantitative detection using a test strip reader. The recoveries of test samples were from 89.26% to 102.02% and the coefficient of variation (%) was less than 4.75%. Parallel analysis of cucumber samples with CTN showed comparable results from the strip test and high performance liquid chromatography. The strip test requires 5-8 min to complete and provides a very useful screening method for the quantitative and qualitative detection of CTN residues in cucumber.
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