Article
Biochemistry & Molecular Biology
Yue Sun, Yan-Ming Yang, Yu-Yu Hu, Lan Ouyang, Zheng-Hua Sun, Xing-Feng Yin, Nan Li, Qing-Yu He, Yang Wang
Summary: This study reveals the impact of SIRT1 inhibition on SIRT1-dependent acetylation in colorectal cancer cells using quantitative acetyl-proteomics. Inhibition of SIRT1 increases protein acetylation in mitochondria, leading to mitochondrial dysfunction and cell apoptosis.
Article
Biochemistry & Molecular Biology
Ioana Stejerean-Todoran, Katharina Zimmermann, Christine S. Gibhardt, Adina Vultur, Christian Ickes, Batool Shannan, Zurine Bonilla del Rio, Anna Wolling, Sabrina Cappello, Hsu-Min Sung, Magdalena Shumanska, Xin Zhang, Maithily Nanadikar, Muhammad U. Latif, Anna Wittek, Felix Lange, Andrea Waters, Patricia Brafford, Jorg Wilting, Henning Urlaub, Dorthe M. Katschinski, Peter Rehling, Christof Lenz, Stefan Jakobs, Volker Ellenrieder, Alexander Roesch, Michael P. Schon, Meenhard Herlyn, Hedwig Stanisz, Ivan Bogeski
Summary: MCUA expression correlates with melanoma patient survival and MCUA knockdown suppresses melanoma cell growth and stimulates migration and invasion. Knockdown of MCUA reduces tumor volumes but promotes lung metastases.
Review
Cell Biology
Grant C. C. Walters, Yuriy M. M. Usachev
Summary: Mitochondria play essential roles in cellular function, including ATP synthesis, reactive oxygen species production, calcium buffering, and apoptotic signaling. In neurons, calcium buffering is particularly important for regulating various calcium-dependent functions. Recent discoveries of molecular components involved in mitochondrial calcium transport have shed light on the roles of mitochondrial calcium regulation in neuronal function and its implications in neurological diseases. This review discusses the multiple roles of mitochondrial calcium uptake and release mechanisms in normal neuronal function and provides insight into the calcium-dependent mechanisms underlying mitochondrial dysfunction in neurological diseases. The targeting of calcium uptake and release mechanisms could potentially lead to novel therapeutic strategies for these diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Physiology
Joanne F. Garbincius, John W. Elrod
Summary: The uptake and extrusion of calcium into and from the mitochondrial matrix have significant effects on cellular metabolism, signaling, and survival. Disruption of mitochondrial calcium cycling is associated with various diseases and inherited neuromuscular disorders. This review provides an overview of the molecular machinery involved in calcium flux across the inner mitochondrial membrane and discusses the physiological implications and potential therapeutic interventions for diseases characterized by aberrant calcium handling. Critical unanswered questions regarding the biology of calcium flux are also highlighted.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Elianne P. Bulthuis, Merel J. W. Adjobo-Hermans, Bastiaan de Potter, Saskia Hoogstraten, Lisanne H. T. Wezendonk, Omar A. Z. Tutakhel, Liesbeth T. Wintjes, Bert van den Heuvel, Peter H. G. M. Willems, Erik-Jan Kamsteeg, M. Estela Rubio Gozalbo, Suzanne C. E. H. Sallevelt, Suzanne M. Koudijs, Joost Nicolai, Charlotte I. de Bie, Jessica E. Hoogendijk, Werner J. H. Koopman, Richard J. Rodenburg
Summary: In this study, the genetic, clinical, and cellular characteristics of two patients with SMDT1 variants and muscle problems were investigated. The variants were found to result in the absence of EMRE protein, formation of MCU subcomplex, and impaired mitochondrial Ca2+ uptake, potentially contributing to the muscle-related symptoms observed in the patients.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Cell Biology
Md Imam Faizan, Tanveer Ahmad
Summary: Classical necroptosis signaling is mediated by death receptors and traditional caspase inhibitory signals, while a non-classical model of necroptosis with relatively unexplored molecular mechanism has emerged, showing close association with mitochondrial dysfunction. Contradictory reports complicate the definitiveness of this emerging model.
Article
Biochemistry & Molecular Biology
Rongjin Yang, Xiaomeng Zhang, Pingping Xing, Shun Zhang, Feiyu Zhang, Jianbang Wang, Jun Yu, Xiaoling Zhu, Pan Chang
Summary: This study demonstrated that Grpel2 expression is upregulated during myocardial ischemia/reperfusion (I/R) injury. Knockdown of Grpel2 exacerbates mitochondrial fission, cardiomyocyte death, and cardiac contractile dysfunction induced by I/R injury. The study also showed that Grpel2 knockdown increases the expression of MCU and mitochondrial calcium content.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Hao Wu, Benjamin Brand, Miriam Eckstein, Sophia M. Hochrein, Magdalena Shumanska, Jan Dudek, Alexander Nickel, Christoph Maack, Ivan Bogeski, Martin Vaeth
Summary: The study demonstrates that mitochondrial Ca2+ uptake is largely dispensable for murine T cell function, despite an increase in cytosolic Ca2+ levels with genetic inactivation of MCU. The discrepancy between this study and previous reports suggests compensatory mechanisms in MCU-deficient mice and/or off-target effects of current MCU inhibitors.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Zhiwang Zhang, Zupeng Luo, Lin Yu, Yang Xiao, Siqi Liu, Zhier ALuo, Zeqiang Ma, Liang Huang, Lianggui Xiao, Mengting Jia, Ziyi Song, Haojie Zhang, Yixing Li, Lei Zhou
Summary: This study investigates the role of mitochondrial calcium uniporter (MCU) in hepatic steatosis induced by high-fat diet (HFD). The results show that inhibition of liver MCU can prevent HFD-induced liver steatosis. The study also reveals that HFD enlarges the mitochondria-associated membranes (MAM) and disrupts mitochondrial calcium homeostasis, leading to liver fat deposition. Inhibition of MAM and MCU can alleviate HFD-induced hepatic steatosis. MCU inhibitors can block MCU channels and reduce mitochondrial calcium levels, providing a potential drug target for the treatment of hepatic steatosis.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Weiser, Aurelle Hermant, Flavien Bermont, Federico Sizzano, Sonia Karaz, Pilar Alvarez-Illera, Jaime Santo-Domingo, Vincenzo Sorrentino, Jerome N. Feige, Umberto De Marchi
Summary: Regulation of mitochondrial redox balance is crucial for cell signaling, but the connection between the redox state and cell conditions is not well understood. The study found that activation of the MCU protein modulates mitochondrial redox state, leading to mitochondrial reduction and improved respiratory capacity and mobility. By bypassing MCU, similar benefits can be achieved by directly reducing mitochondrial proteins. This research demonstrates the importance of MCU in regulating mitochondrial redox balance and its effects on respiratory and mobility functions.
Article
Microbiology
Angela M. A. Velasquez, Paula J. Bartlett, Irwin A. P. Linares, Thais G. Passalacqua, Daphne D. L. Teodoro, Kely B. Imamura, Stela Virgilio, Luiz R. O. Tosi, Aline de Lima Leite, Marilia A. R. Buzalaf, Jecika M. Velasques, Adelino V. G. Netto, Andrew P. Thomas, Marcia A. S. Graminha
Summary: The study elucidates the mechanisms of action of an antileishmanial complex, which induces oxidative stress, cell cycle arrest, and apoptotic-like features in the parasite. Additionally, the complex shows effectiveness in reducing parasite load in Leishmania-infected rodents, suggesting its potential application in treating both cutaneous and visceral forms of the disease.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biology
Giampaolo Morciano, Natalia Naumova, Piotr Koprowski, Sara Valente, Vilma A. Sardao, Yaiza Potes, Alessandro Rimessi, Mariusz R. Wieckowski, Paulo J. Oliveira
Summary: This review summarizes the intriguing phenomenon of the mitochondrial permeability transition pore (mPTP) in cell biology. Despite almost 50 years of research, the mechanisms of mPTP are still not definitively understood. From initially being considered an in vitro artifact to now being recognized for its physiological and pathological implications, the journey of mPTP research has been long and complex.
BIOLOGICAL REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Veronica Costiniti, Guilherme H. S. Bomfim, Maria Neginskaya, Ga-Yeon Son, Erna Mitaishvili, Marta Giacomello, Evgeny Pavlov, Rodrigo S. Lacruz
Summary: This study analyzed the functional features of mitochondria in rat ameloblasts and found an increased mitochondrial Ca2+ handling in the maturation stage, particularly in response to Ca2+ influx via store-operated Ca2+ entry (SOCE). These findings provide insights into the role of mitochondria in enamel and suggest that SOCE and mitochondrial Ca2+ uptake are complementary processes in biological mineralization.
Article
Cell Biology
Taylor E. Huntington, Rahul Srinivasan
Summary: The study revealed unique properties of astrocytic mitochondria in subcellular morphology, mechanisms of calcium influx, and responses to neurotransmitter receptor agonists in the adult mouse brain.
Review
Biochemistry & Molecular Biology
Linlin Zhang, Jingyi Qi, Xu Zhang, Xiya Zhao, Peng An, Yongting Luo, Junjie Luo
Summary: Mitochondria are essential for cellular energy metabolism and the generation of oxygen free radicals, and also play a crucial role in cell death. Ca2+ acts as a messenger for cell proliferation and death, and is involved in the energy metabolism, physiology, and pathology of mitochondria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Carmen Falcone, Elisa Penna, Tiffany Hong, Alice F. Tarantal, Patrick R. Hof, William D. Hopkins, Chet C. Sherwood, Stephen C. Noctor, Veronica Martinez-Cerdeno
Summary: Interlaminar astrocytes (ILAs) are a unique subset of cortical astrocytes found in layer I, originating likely from radial glial (RG) cells, proliferating locally during gestation, and extending interlaminar processes postnatally. Studies on three primate species and mice revealed that ILAs exhibit increased density and morphological complexity with age, express multiple markers including RG and astrocyte markers, and show differences between primate species and mice. These findings suggest a lineal relationship between RG cells and ILAs.
Article
Cell Biology
Simona Feno, Rosario Rizzuto, Anna Raffaello, Denis Vecellio Reane
Summary: The discovery of the Mitochondrial Calcium Uniporter (MCU) gene in 2011 led to an explosion of studies on the composition, regulation, and pathophysiological roles of the MCU complex. Recent research has provided new insights into the molecular structure and composition of the MCU complex, as well as the mechanisms that regulate MCU channel activity.
Article
Neurosciences
Alice F. Tarantal, Dennis J. Hartigan-O'Connor, Elisa Penna, Anna Kreutz, Michele L. Martinez, Stephen C. Noctor
Summary: Inoculating Zika virus in early gestation has lasting effects on fetal cortical structure, leading to abnormal development of the fetal brain including changes in microglial cells, blood vessels, and the cortical plate.
Article
Neurosciences
Elisa Penna, Jon M. Mangum, Hunter Shepherd, Veronica Martinez-Cerdeno, Stephen C. Noctor
Summary: The study reveals that endothelial cells in developing cortical vessels extend numerous fine processes that directly contact mitotic NPCs and microglia, indicating a complex interconnected environment near the ventricle.
Article
Biochemistry & Molecular Biology
Elena Fernandez-Delgado, Samuel Estirado, Javier Espino, Emilio Vinuelas-Zahinos, Francisco Luna-Giles, Ana B. Rodriguez Moratinos, Jose A. Pariente
Summary: One of the commonly used strategies for drug development is the coordination of bioactive ligands to transition metals. In this study, four Pt(II) complexes were synthesized and characterized, and their anticancer abilities were investigated in different cancer cell lines. The results showed that modulating the lipophilicity of the ligands can help improve the cytotoxic effect of the metal complexes.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Plant Sciences
Enrico Cortese, Roberto Moscatiello, Francesca Pettiti, Luca Carraretto, Barbara Baldan, Lorenzo Frigerio, Ute C. Vothknecht, Ildiko Szabo, Diego De Stefani, Marisa Brini, Lorella Navazio
Summary: This study successfully achieved precise measurements of dynamic changes in free Ca2+ concentration in the lumen of the plant endoplasmic reticulum using an aequorin chimera with reduced Ca2+ affinity, revealing rapid and transient increases of the ER luminal Ca2+ concentration in response to different environmental stresses. Comparative analysis also indicates a complex interplay between ER and chloroplast Ca2+ dynamics in shaping cytosolic Ca2+ signals during signal transduction events.
Article
Medicine, General & Internal
Daniela Basso, Ada Aita, Filippo Navaglia, Paola Mason, Stefania Moz, Alessio Pinato, Barbara Melloni, Luca Iannelli, Andrea Padoan, Chiara Cosma, Angelo Moretto, Alberto Scuttari, Daniela Mapelli, Rosario Rizzuto, Mario Plebani
Summary: Salivary-based surveillance with contact tracing effectively allowed to limit SARS-CoV-2 contagion, also in a population with a high incidence.
Article
Neurosciences
Sara Montagnese, Lisa Zarantonello, Chiara Formentin, Gianluca Giusti, Chiara Mangini, Cheryl M. Isherwood, Paolo Ferrari, Antonio Paoli, Daniela Mapelli, Rosario Rizzuto, Stefano Toppo, Debra J. Skene, Roberto Vettor, Rodolfo Costa
Summary: The study aimed to assess sleep quality and timing in a group of university students and evaluate the effects of a circadian hygiene education initiative. It was found that most students had poor sleep quality, but those who joined during the lockdown period had better sleep quality. The circadian hygiene advice influenced students' wake-up times significantly but did not affect sleep duration. The events in 2020 had a significant impact on sleep quality and timing.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Enrico Cortese, Alessandro Galenda, Alessia Famengo, Luca Cappellin, Marco Roverso, Alessio G. Settimi, Manuele Dabala, Diego De Stefani, Alessandro Fassina, Gianluigi Serianni, Vanni Antoni, Lorella Navazio
Summary: Non-thermal plasma technology is increasingly being used in the field of plant biology. This study investigated the mechanisms of plant perception of plasma-activated water (PAW) and found that it triggers intracellular calcium signals in plants. The occurrence and characteristics of these signals depend on the operational conditions and chemical composition of PAW. These findings have important implications for using PAW to induce stress resilience in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Correction
Cell Biology
Roman Serrat, Ana Covelo, Vladimir Kouskoff, Sebastien Delcasso, Andrea Ruiz-Calvo, Nicolas Chenouard, Carol Stella, Corinne Blancard, Benedicte Salin, Francisca Julio-Kalajzic, Astrid Cannich, Federico Massa, Marjorie Varilh, Severine Deforges, Laurie M. Robin, Diego De Stefani, Arnau Busquets-Garcia, Frederic Gambino, Anna Beyeler, Sandrine Pouvreau, Giovanni Marsicano
Article
Multidisciplinary Sciences
Enrico Zampese, David L. Wokosin, Patricia Gonzalez-Rodriguez, Jaime N. Guzman, Tatiana Tkatch, Jyothisri Kondapalli, William C. Surmeier, Karis B. D'Alessandro, Diego De Stefani, Rosario Rizzuto, Masamitsu Iino, Jeffery D. Molkentin, Navdeep S. Chandel, Paul T. Schumacker, D. James Surmeier
Summary: This study investigates the mechanism of how dopaminergic neurons sustain spike activity by stimulating mitochondrial oxidative phosphorylation through two complementary calcium-dependent mechanisms. However, this control mechanism also leads to increased oxidative stress in the neurons and may contribute to their decline with aging and disease.
Article
Biochemistry & Molecular Biology
Samuel Estirado, Elena Fernandez-Delgado, Emilio Vinuelas-Zahinos, Francisco Luna-Giles, Ana B. Rodriguez, Jose A. Pariente, Javier Espino
Summary: This study evaluated the antitumoral potential of PtDPhPzTn against TNBC cells, showing enhanced cytotoxicity compared to cisplatin and PtPzTn, inducing apoptosis, causing S phase arrest, and impairing cell migration. Melatonin was found to enhance the anticancer effects of PtDPhPzTn, increasing apoptosis and inhibiting cell migration when used together.
Meeting Abstract
Cardiac & Cardiovascular Systems
Nicola Moro, Salvatore Antonucci, Karin Hammer, Antonio Campoa, Giulia Borile, Pierluigi Carullo, Paola Pescef, Riccardo Bariani, Barbara Bauce, Rosario Rizzuto, Diego De Stefani, Cristina Mammucari, Daniele Catalucci, Lars Maier, Fabio Di Lisa, Tania Zaglia, Marco Mongillo
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Multidisciplinary Sciences
Samantha Corra, Vanessa Checchetto, Michele Brischigliaro, Chiara Rampazzo, Emanuela Bottani, Cristina Gagliani, Katia Cortese, Cristiano De Pitta, Marco Roverso, Diego De Stefani, Sara Bogialli, Massimo Zeviani, Carlo Viscomi, Ildiko Szabo, Rodolfo Costa
Summary: Mutations in MPV17 contribute to mitochondrial DNA depletion syndromes, and this study shows that dMpv17 plays a role in mtDNA maintenance and energy homeostasis in Drosophila melanogaster.
Article
Cell Biology
Dominic Tscherrig, Rajesh Bhardwaj, Daniel Biner, Jan Dernic, Daniela Ross-Kaschitza, Christine Peinelt, Matthias A. Hediger, Martin Lochner
Summary: Changes in Ca2+ levels regulate many physiological functions, and the endoplasmic reticulum is the major Ca2+ store in cells. Store-operated calcium entry (SOCE) is a refilling process that involves protein-protein interactions between Ca2+ sensing STIM in the ER and Orai proteins in the plasma membrane, forming selective Ca2+ channels. Abnormal SOCE is associated with diseases and cancers, making STIM and Orai important therapeutic targets. Novel GSK-7975A-based molecular probes have been developed for studying SOCE in different cells and settings.
Article
Cell Biology
Nicola Fameli, Cornelis van Breemen, Klaus Groschner
Summary: The study provides evidence for the importance of junctional membrane architecture in cellular function. Through modeling and prediction, it is shown that nano-scale membrane spacing plays a crucial role in junctional ER Ca2+ refilling and signaling efficiency. Additionally, interactions between Ca2+ and the phospholipid membrane surface are suggested to support interfacial Ca2+ transport and receptor targeting. This research introduces a new concept in pathophysiology.
Article
Cell Biology
Satomi Ohtsuka, Yumi Miyai, Hiroyuki Mima, Masaki Magari, Yoichi Chiba, Futoshi Suizu, Hiroyuki Sakagami, Masaki Ueno, Hiroshi Tokumitsu
Summary: This study characterized the splice variants of mouse CaMKKI3/2 and found that CaMKKI3-3 and I3-3x were functionally active and likely to be bona fide CaMKIV kinases involved in the regulation of spermiogenesis.