Article
Engineering, Multidisciplinary
Di Wang, Katarina Madunic, Tao Zhang, Guinevere S. M. Lageveen-Kammeijer, Manfred Wuhrer
Summary: Aberrant glycosylation is a hallmark of colorectal cancer, and this study reveals the distinct N-glycomic profiles of different cell types in CRC tissues. Cancer cells show increased expression of certain N-glycan structures and decreased expression of others compared to normal mucosa, while stromal cells exhibit different expression patterns.
Article
Biochemistry & Molecular Biology
Vidhya A. Nair, Lara J. Bou Malhab, Wael M. Abdel-Rahman
Summary: BPA has carcinogenic effects on colon cancer cells, increasing cellular invasiveness and growth, and activating multiple potential carcinogenic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Caryl Evans, Kathryn Howells, Rie Suzuki, Alastair J. H. Brown, Helen M. Cox
Summary: This study investigated the effects of capsaicin and allyl isothiocyanate on the gastrointestinal tract. It was found that they activate TRPV1 and TRPA1, leading to ion secretion and transport in the GI mucosa. The results also showed that TRPV1 signaling is regulated by submucosal neurons and epithelial NK1 receptor activation, while TRPA1 signaling is regulated by endogenous prostaglandins and EP4 receptor activation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Oncology
Kunpeng Lv, Ruochen Li, Yifan Cao, Yun Gu, Xin Liu, Xudong He, Kaifeng Jin, Hanji Fang, Yuchao Fei, Mingsu Shi, Hao Liu, He Li, Hongyong He, Chao Lin, Heng Zhang, Jiejie Xu
Summary: LAG-3, a transmembrane immune checkpoint, plays a role in immune escape in gastric cancer by suppressing T-cell-mediated anti-tumor immunity. Our study showed that LAG-3 expression was associated with poorer clinical outcomes in EBV-positive and MLH1-defective gastric cancer, suggesting it as a potential immunotherapeutic target.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Review
Multidisciplinary Sciences
Melanie Haas Kucherlapati
Summary: This study examines important mouse models in colorectal cancer (CRC) and discusses their strengths and weaknesses. It also evaluates how these models can be used by researchers in the future. The study highlights the significance of the immune system and tumor microenvironments in CRC progression and treatment.
Article
Biology
Siying Li, Ran Lu, Linjuan Shu, Yulin Chen, Jin Zhao, Junlong Dai, Qiaorong Huang, Xue Li, Wentong Meng, Feiwu Long, Yuan Li, Chuanwen Fan, Zongguang Zhou, Xianming Mo
Summary: This study identifies different populations of fibroblasts and myofibroblasts in human colon mucosa and colon cancer tissues based on gene expression profiles. The compositions and gene expression patterns of these cells were found to be highly altered in colon cancer tissue.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ping Li, Yang Yang, Xinyu Yang, Yifei Wang, Chon-Kit Chou, Mengmeng Jiang, Jingbin Zheng, Fengyang Chen, Xin Chen
Summary: The study found that TNF receptor type II (TNFR2) is expressed in a wide range of tumor cells. Knocking out TNFR2 in mouse colon cancer cells MC38 and CT26 using CRISPR/Cas9 technology inhibited in vitro proliferation and colony formation of cancer cells through inhibition of AKT phosphorylation and promotion of autophagy-induced cell death. In addition, TNFR2 deficiency significantly impaired the in vivo growth of MC38 or CT26 tumors in mice, accompanied by a decrease in soluble TNFR2 levels in the circulation and an increase in the number of tumor-infiltrating IFN gamma+ CD8 cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Anil K. Giri
Summary: The study reveals a negative correlation between ETV4 expression and DNA methylation, and a positive correlation between ETV5 expression and DNA methylation in colon cancer tissue. Additionally, higher ETV5 expression is associated with shorter relapse-free survival in colon cancer patients receiving adjCTX treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Michael D. Toboni, Sharon Wu, Alex Farrell, Joanne Xiu, Jennifer R. Ribeiro, Matthew J. Oberley, Rebecca Arend, Britt K. Erickson, Thomas J. Herzog, Premal H. Thaker, Matthew A. Powell
Summary: This study aims to identify differential survival outcomes and immune checkpoint inhibitor response in MLH1 hypermethylated versus MLH1 mutated endometrial tumors and determine whether their molecular profiles can elucidate the differential outcomes.
GYNECOLOGIC ONCOLOGY
(2023)
Article
Oncology
Julia Matas, Brendan Kohrn, Jeanne Fredrickson, Kelly Carter, Ming Yu, Ting Wang, Xianyong Gui, Thierry Soussi, Victor Moreno, William M. Grady, Miguel A. Peinado, Rosa Ana Risques
Summary: This study reveals that mutations in common colorectal cancer genes can be detected in the normal colon, highlighting the prevalent somatic evolution in patients with colorectal cancer. Somatic evolution contributes to clonal expansions in the normal colon, especially in individuals with early-onset colorectal cancer.
Article
Immunology
Mihaly Balogh, Jixiang Zhang, Caitlyn M. Gaffney, Neha Kalakuntla, Nicholas T. Nguyen, Ronnie T. Trinh, Clarissa Aguilar, Hoang Vu Pham, Bojana Milutinovic, James M. Nichols, Rajasekaran Mahalingam, Andrew J. Shepherd
Summary: This study demonstrates that colon cancer is associated with latent sensory neuron dysfunction and implicates cytokine/chemokine signaling in this process. These findings may have implications for determining risk factors and treatment responsiveness related to neuropathy in colorectal cancer.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Amr Amin, Aaminah Farrukh, Chandraprabha Murali, Akbar Soleimani, Francoise Praz, Grazia Graziani, Hassan Brim, Hassan Ashktorab
Summary: The study revealed that saffron has a significant anti-proliferative effect on colorectal cancer cells with deficient MMR, which may have therapeutic implications for MSI CRC patients who are generally not recommended for 5-fluorouracil-based treatment.
Article
Pathology
Mezgebe Gebrekiristos, Joshua Melson, Alice Jiang, Lela Buckingham
Summary: This study investigated the role of epigenetic changes in early colon tumorigenesis. Dysregulation of DNA methylation patterns and miRNA expression were observed in precancerous lesions, suggesting their involvement in the development of colon cancer. The findings highlight the potential diagnostic and therapeutic targets in early stages of cancer development.
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2022)
Article
Oncology
Rhonda M. Brand, Beth Dudley, Eve Karloski, Ashley Zyhowski, Rebecca Raphael, Danielle Pitlor, E. Jeffrey Metter, Reet Pai, Kenneth Lee, Randall E. Brand, Shikhar Uttam
Summary: This study examined the differences in immune signatures among LS patients, non-LS patients, and patients with and without a history of colorectal cancer. The findings suggest the existence of immune features specific to LS-status and CRC history, which may help in assessing CRC risk in individuals with LS and identifying prevention targets.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Lynn Hakki, Asama Khan, Mithat Gonen, Zsofia Stadler, Neil H. Segal, Jinru Shia, Maria Widmar, Iris H. Wei, J. Joshua Smith, Emmanouil P. Pappou, Garrett M. Nash, Philip B. Paty, Julio Garcia-Aguilar, Martin R. Weiser
Summary: In contrast to microsatellite stable (MSS) colon cancer, lymph node metastases are less prevalent in microsatellite instability (MSI) colon cancer, but the factors associated with lymph node metastases are similar to MSS colon cancer. In N2 disease, MSI patients have a lower rate of 3-year recurrence-free survival compared to MSS patients.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Editorial Material
Cell & Tissue Engineering
Masahito Yoshihara, Magdalena Wagner, Anastasios Damdimopoulos, Cheng Zhao, Sophie Petropoulos, Shintaro Katayama, Juha Kere, Fredrik Lanner, Pauliina Damdimopoulou
Summary: Ovaries play a crucial role in the development, fertility, and reproduction of women. They age faster than other tissues and their function is lost before other organs undergo senescence. While there have been debates about the presence of germline stem cells and neo-oogenesis in adult human ovaries, our recent analysis did not find evidence of such stem cells. However, we welcome discussions on different protocols and acknowledge the importance of excellence in methods.
Article
Immunology
Elina A. Tuovinen, Outi Kuismin, Leila Soikkonen, Timi Martelius, Meri Kaustio, Sari Hamalainen, Hanna Viskari, Jaana Syrjanen, Ulla Wartiovaara-Kautto, Kari K. Eklund, Janna Saarela, Markku Varjosalo, Juha Kere, Timo Hautala, Mikko R. J. Seppanen
Summary: The NF-κB family of transcription factors is important in cellular signaling pathways, and NFKB1 variants have been associated with CVID and immunodeficiency. However, the penetrance and prevalence of CVID are relatively low, while inflammatory manifestations are more common.
CLINICAL IMMUNOLOGY
(2023)
Letter
Cell & Tissue Engineering
Masahito Yoshihara, Magdalena Wagner, Anastasios Damdimopoulos, Cheng Zhao, Sophie Petropoulos, Shintaro Katayama, Juha Kere, Fredrik Lanner, Pauliina Damdimopoulou
Article
Multidisciplinary Sciences
Daniel F. Kaemena, Masahito Yoshihara, Meryam Beniazza, James Ashmore, Suling Zhao, Marten Bertenstam, Victor Olariu, Shintaro Katayama, Keisuke Okita, Simon R. Tomlinson, Kosuke Yusa, Keisuke Kaji
Summary: CRISPR/Cas9-mediated genome-wide knockout screening identified 24 reprogramming roadblock genes, among which depletion of the predicted KRAB zinc finger protein Zfp266 consistently enhanced murine iPSC generation in various reprogramming settings. ZFP266 was found to bind to Short Interspersed Nuclear Elements (SINEs) near the binding sites of OCT4, SOX2, and KLF4, inhibiting chromatin opening. Replacement of the KRAB co-suppressor with co-activator domains transformed ZFP266 from an inhibitor to a potent facilitator of iPSC reprogramming. The interaction between SINE and KRAB-ZFP is proposed to be a critical regulator of chromatin accessibility for efficient cellular identity changes.
NATURE COMMUNICATIONS
(2023)
Article
Cardiac & Cardiovascular Systems
Jaakko S. Tyrmi, Tea Kaartokallio, A. Inkeri Lokki, Tiina Jaaskelainen, Eija Kortelainen, Sanni Ruotsalainen, Juha Karjalainen, Samuli Ripatti, Anna Kivioja, Triin Laisk, Johannes Kettunen, Anneli Pouta, Katja Kivinen, Eero Kajantie, Seppo Heinonen, Juha Kere, Hannele Laivuori
Summary: This study used a genome-wide association study to uncover the genetic factors underlying preeclampsia and maternal hypertension during pregnancy. The analysis identified several novel genetic associations and found that genes related to blood pressure traits are also associated with preeclampsia. These findings suggest a connection between cardiovascular function and the development of preeclampsia.
Article
Multidisciplinary Sciences
Lisa Gawriyski, Eeva-Mari Jouhilahti, Masahito Yoshihara, Liangru Fei, Jere Weltner, Tomi T. Airenne, Ras Trokovic, Shruti Bhagat, Mari H. Tervaniemi, Yasuhiro Murakawa, Kari Salokas, Xiaonan Liu, Sini Miettinen, Thomas R. Burglin, Biswajyoti Sahu, Timo Otonkoski, Mark S. Johnson, Shintaro Katayama, Markku Varjosalo, Juha Kere
Summary: LEUTX, a transcription factor, is expressed in human preimplantation embryos at the 4-8 cell stages and silenced in somatic tissues. Through multiomic characterization, we found that LEUTX interacts with histone acetyltransferases, EP300 and CBP, via its 9 amino acid transactivation domain. It targets genomic cis-regulatory sequences overlapping with repetitive elements and functions as a transcriptional activator, upregulating genes involved in preimplantation development and 8-cell-like markers.
Article
Cell Biology
Andrea Bieder, Gayathri Chandrasekar, Arpit Wason, Steffen Erkelenz, Jay Gopalakrishnan, Juha Kere, Isabel Tapia-Paez
Summary: This study discovered the physical protein-protein interaction between DYX1C1 and DCDC2 genes, as well as their interactions with the centrosomal protein CPAP. It also found a synergistic genetic interaction between dyx1c1 and dcdc2b in zebrafish, exacerbating the ciliary phenotype. Furthermore, a mutual effect on transcriptional regulation was observed between DYX1C1 and DCDC2 in a cellular model.
BMC MOLECULAR AND CELL BIOLOGY
(2023)
Article
Genetics & Heredity
Julia E. Niskanen, Asa Ohlsson, Ingrid Ljungvall, Michaela Droegemueller, Robert F. Ernst, Dennis Dooijes, Hanneke W. M. van Deutekom, J. Peter van Tintelen, Christian J. B. Snijders Blok, Marion van Vugt, Jessica van Setten, Folkert W. Asselbergs, Aleksandra Domanjko Petric, Milla Salonen, Sruthi Hundi, Matthias Hoertenhuber, DoGA Consortium, Juha Kere, W. Glen Pyle, Jonas Donner, Alex V. Postma, Tosso Leeb, Goran Andersson, Marjo K. Hytonen, Jens Haggstrom, Maria Wiberg, Jana Friederich, Jenny Eberhard, Magdalena Harakalova, Frank G. van Steenbeek, Gerhard Wess, Hannes Lohi
Summary: This study used a canine model of dilated cardiomyopathy (DCM) to perform genome-wide analysis and identified two novel candidate genes associated with DCM. It also revealed the complex genetic factors involved in the disease, including cardiac function, energy homeostasis, and morphology. Additionally, the study found candidate variants in these two genes in human DCM patients.
Article
Cell & Tissue Engineering
Masahito Yoshihara, Juha Kere
Summary: This study summarizes the methods of classifying and analyzing a type of cells in human embryos using single-cell RNA sequencing technology, which will facilitate further research on gene activation and early development in human embryos.
Article
Biochemical Research Methods
Nina Boskovic, Gamze Yazgeldi, Sini Ezer, Mari H. Tervaniemi, Jose Inzunza, Spyridon Panagiotis Deligiannis, Baris Yasar, Tiina Skoog, Kaarel Krjutskov, Shintaro Katayama, Juha Kere
Summary: This article presents a modified single-cell tagged reverse transcription protocol for studying gene expression at the single-cell level or with limited RNA input. The authors describe various enzymes, a modified lysis buffer, and additional clean-up steps before cDNA amplification. They also provide an optimized single-cell RNA sequencing method for studying mammalian preimplantation development using handpicked single cells or tens to hundreds of cells as input material. For complete details on the use and execution of this protocol, please refer to Ezer et al.1.
Article
Cell Biology
Anniina Pirttiniemi, Krishna Adeshara, Natalie Happonen, Elisabet Einarsdottir, Shintaro Katayama, Hanne Salmenkari, Sohvi Horkko, Juha Kere, Per-Henrik Groop, Markku Lehto
Summary: Inorganic polyphosphates are bioactive phosphate polymers found in all living organisms, and in mammals, they play a vital role in regulating cellular metabolism, coagulation, and inflammation. Long-chain polyphosphates, particularly P700, can modulate the type I interferon signaling and cytokine secretion in human leukocytes. P700 treatment decreases interferon signaling and subsequent gene expression, while augmenting the secretion of various cytokines. This study demonstrates the extensive modulatory effects of P700 on cytokine signaling, specifically targeting type I interferon signaling in human leukocytes.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Baris Yasar, Nina Boskovic, Eeva-Mari Jouhilahti, Piibe Vill, Thomas Burglin, Shintaro Katayama, Tonis Org, Juha Kere, Ants Kurg
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
