Article
Biochemistry & Molecular Biology
Yijie Wang, Xiong Liu, Min Wang, Yu Wang, Shuo Wang, Lai Jin, Min Liu, Jun Zhou, Yan Chen
Summary: This study reveals that UBE3B is an E3 ligase for HIF-2 & alpha; and its depletion inhibits breast cancer progression. UBE3B physically interacts with HIF-2 & alpha; and promotes its polyubiquitination, thereby inhibiting its degradation. UBE3B depletion also inhibits breast cancer cell proliferation, migration, and invasion, and suppresses tumor growth and metastasis.
Article
Biochemistry & Molecular Biology
Yijie Wang, Xiong Liu, Min Liu, Yu Wang, Shuo Wang, Lai Jin, Min Liu, Jun Zhou, Yan Chen
Summary: It has been found that UBE3B acts as an E3 ligase for HIF-2 alpha, promoting its polyubiquitination and inhibiting its degradation, which plays a crucial role in breast cancer growth and metastasis.
Review
Oncology
Karolina Kozal, Anna Krzeslak
Summary: Hypoxia-inducible factors play an important role in breast cancer, affecting immune response, metabolism, angiogenesis, invasion, and metastasis. The study suggests that targeting hypoxia-inducible factors in breast cancer therapy should take into account multiple factors and requires a better understanding of their role in this type of cancer.
Article
Cell Biology
Zhiguo Niu, Jianing Fan, Fengzhe Chen, Huijie Yang, Xin Li, Ting Zhuang, Chunlei Guo, Qi Cao, Jian Zhu, Hui Wang, Qingsong Huang
Summary: Breast cancer is the most common malignancy in women globally. Abnormal expression of RBCK1 protein in breast tumors makes it a valuable diagnostic marker and drug target. Hypoxic regions in breast cancer activate the HIF1 pathway, influencing tumor recurrence and metastasis. Targeting RBCK1 could be a promising treatment strategy for ER-positive breast cancer.
CELL DEATH & DISEASE
(2022)
Review
Cell Biology
Minghui Zhang, Yu Zhang, Yubo Ding, Jialu Huang, Jingwei Yao, Zhuoyi Xie, Yufan Lv, Jianhong Zuo
Summary: This review examines the regulatory roles of HIF-1 alpha and lncRNAs in the EMT process of tumor cells and their relationship with tumorigenesis, and summarizes current drugs that inhibit tumor progression by modulating HIF-1 alpha.
Article
Oncology
Leah Davis, Matthias Recktenwald, Evan Hutt, Schuyler Fuller, Madison Briggs, Arnav Goel, Nichole Daringer
Summary: Hypoxia, or inadequate oxygen supply, is a characteristic of the tumor microenvironment and is associated with a poor prognosis and resistance to therapy. HIF-alpha, specifically the HIF-1 alpha and HIF-2 alpha subunits, play a crucial role in mediating the transcription of important proteins involved in tumor progression. While HIF-1 alpha has been extensively studied, the role of HIF-2 alpha is often overlooked. HIF-2 alpha can enhance the aggressive behavior of tumors, promoting metastasis, tissue remodeling, angiogenesis, and the upregulation of cancer stem cell factors. This review highlights the structure, function, hypoxic response, and roles of HIF-2 alpha in cancer progression, as well as discussing current therapeutic strategies and future directions.
Article
Biochemistry & Molecular Biology
Elena Monti, Alessandro Mancini, Emanuela Marras, Marzia Bruna Gariboldi
Summary: Experimental evidence suggests that mitochondrial ROS plays a pivotal role in hypoxia-induced Epithelial-Mesenchymal Transition (EMT), and inhibiting mitochondrial ROS production could reverse EMT. UQCRB is a key factor in hypoxic signaling-induced EMT and may serve as a new therapeutic target for drug development.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Review
Cell Biology
Nasim Kheshtchin, Jamshid Hadjati
Summary: Hypoxia, a common characteristic of solid tumors, contributes to different aspects of tumor progression and limits the efficacy of immunotherapies. Developing new immunotherapy strategies involving therapeutic targeting of HIF-1 molecules associated with hypoxia may enhance the clinical effectiveness of immunotherapy. Targeting hypoxia presents a potential opportunity to improve the clinical benefit of cancer immunotherapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Oncology
Liyun Yong, Shasha Tang, Haixin Yu, Hongyi Zhang, Yi Zhang, Yuan Wan, Fengfeng Cai
Summary: Breast cancer is the most common cancer in women, and drug resistance is a persistent clinical challenge. Hypoxia-inducible Factor-1 Alpha (HIF-1α) plays a crucial role in regulating cellular response to hypoxia and is closely associated with multidrug resistance in breast cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biology
Takeharu Sakamoto, Yuya Fukui, Yasumitsu Kondoh, Kaori Honda, Takeshi Shimizu, Toshiro Hara, Tetsuro Hayashi, Yurika Saitoh, Yoshinori Murakami, Jun-ichiro Inoue, Shuichi Kaneko, Hiroyuki Osada, Motoharu Seiki
Summary: The study identifies naphthofluorescein as a Mint3 inhibitor that disrupts the Mint3-FIH-1 interaction and attenuates HIF-1 activity. In vivo experiments in mice show a reduction in tumor growth with attenuated inflammatory cytokine production and endotoxic shock, providing a potential option for targeted therapies for cancer and inflammatory diseases that avoid severe adverse effects.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yejiao Ruan, Lingyun He, Jiamin Chen, Jinfeng Wang, Shujing Zhao, Xiaoling Guo, Yao Xie, Zhenzhai Cai, Xian Shen, Chao Li
Summary: There is still a lack of convenient ways to simulate the in vivo hypoxic tumor microenvironment (TME) under normoxia in vitro. In this study, a three-dimensional culture system with a core-shell structure (3d-ACS) was established to simulate the hypoxic TME. The gastric cancer cells formed organoid-like structures in the 3d-ACS and showed more aggressive growth and decreased drug responses.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Ping Liu, Huiqiong Huang, Xiaorong Qi, Ce Bian, Meng Cheng, Lili Liu, Luqi Xue, Xia Zhao, Tao Yi, Yi Quan
Summary: The lncRNA-MIR210HG is induced by hypoxia in ovarian cancer, mainly located in the cytosol, and promotes cancer progression by inhibiting HIF-1 alpha degradation. Its upregulation is associated with tumor progression and poor prognosis in ovarian cancer patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Yongkang Yang, Chelsey Chen, Qiaozhu Zuo, Haiquan Lu, Shaima Salman, Yajing Lyu, Tina Yi-Ting Huang, Elizabeth E. Wicks, Walter Jackson III, Emmanuel Datan, Ru Wang, Yufeng Wang, Nguyet Le, Yayun Zhu, Wenxin Qin, Gregg L. Semenza
Summary: Hypoxia in the breast cancer microenvironment leads to increased expression of HIF-1, which is associated with poor patient outcome. In this study, NARF was identified as a hypoxia-inducible, HIF-1 target gene in human breast cancer cells. Knockdown of NARF significantly reduced the population of breast cancer stem cells and impaired tumor initiation capacity and lung metastasis in mouse models.
Article
Biotechnology & Applied Microbiology
Jing Sun, Shuai Zhu
Summary: Through coexpression analysis and the LncTarD database, a set of hypoxia-related long noncoding RNAs (lncRNAs) were identified, and a prognostic model was constructed. This model showed excellent performance in predicting the prognosis of pancreatic cancer patients. Overexpression of TSPOAP1-AS1, a hypoxia-related lncRNA, attenuated the proliferation and invasion of pancreatic cancer cells. HIF-1a bound to the promoter of TSPOAP1-AS1 and impaired its transcription under hypoxia. In conclusion, the hypoxia-related lncRNA assessment model could be a potential strategy for prognostic prediction in pancreatic cancer. The fourteen lncRNAs contained in the model could be helpful for uncovering the mechanisms of pancreatic tumorigenesis.
Article
Oncology
Carla Fourie, Manisha du Plessis, Justin Mills, Anna -Mart Engelbrecht
Summary: This study explores the role of HIF-1 alpha in breast cancer cells under hypoxic conditions and its impact on cell survival, apoptosis, and autophagy. The results suggest that targeting HIF-1 alpha may sensitize breast cancer cells to doxorubicin treatment and improve treatment responses. Silencing the HIF-1 alpha gene expression attenuates treatment resistance in a hypoxic environment.
EXPERIMENTAL CELL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Christian Bates, Simon J. Hubbard, Mark P. Ashe
BIOCHEMICAL SOCIETY TRANSACTIONS
(2018)
Article
Oncology
Henry J. Pegg, Hannah Harrison, Connor Rogerson, Paul Shore
MOLECULAR CANCER RESEARCH
(2019)
Article
Cell Biology
Mariavittoria Pizzinga, Christian Bates, Jennifer Lui, Gabriella Forte, Fabian Mores-Polanco, Emma Linney, Barbora Knotkova, Beverley Wilson, Clara A. Solari, Luke E. Berchowitz, Paula Portela, Mark P. Ashe
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
J. C. Ashworth, J. L. Thompson, J. R. James, C. E. Slater, S. Pijuan-Galito, K. Lis-Slimak, R. J. Holley, K. A. Meade, A. Thompson, K. P. Arkill, M. Tassieri, A. J. Wright, G. Farnie, C. L. R. Merry
Article
Biochemistry & Molecular Biology
Feixia Peng, Ying Zhou, Juan Wang, Baoqiang Guo, Yun Wei, Huan Deng, Zihui Wu, Cheng Zhang, Kaituo Shi, Yuan Li, Xin Wang, Paul Shore, Shasha Zhao, Wensheng Deng
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Ran Ran, Hannah Harrison, Nur Syamimi Ariffin, Rahna Ayubi, Henry J. Pegg, Wensheng Deng, Andrea Mastro, Penny D. Ottewell, Susan M. Mason, Karen Blyth, Ingunn Holen, Paul Shore
Article
Multidisciplinary Sciences
Claire A. Walsh, Nina Akrap, Elena Garre, Ylva Magnusson, Hannah Harrison, Daniel Andersson, Emma Jonasson, Svanheidur Rafnsdottir, Hani Choudhry, Francesca Buffa, Jiannis Ragoussis, Anders Stahlberg, Adrian Harris, Goran Landberg
Article
Microbiology
Jamie L. Thompson, Angela Downie Ruiz Velasco, Alice Cardall, Rebecca Tarbox, Jaineeta Richardson, Gemma Clarke, Michelle Lister, Hannah C. Howson-Wells, Vicki M. Fleming, Manjinder Khakh, Tim Sloan, Nichola Duckworth, Sarah Walsh, Chris Denning, C. Patrick McClure, Andrew V. Benest, Claire H. Seedhouse
Summary: The study evaluated alternative commercially available kits for SARS-CoV-2 genome detection and found that using heat inactivation in an extraction-free protocol could reduce the time from sample receipt to test outcome. The results demonstrate that sample treatment can significantly impact the performance of diagnostic kits, with different effects depending on the kit used.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Medicine, General & Internal
Helen Clarke, Michelle Harvie, Cheryl Lombardelli, Suzanne Krizak, Katharine Sellers, Hannah Harrison, Yit Y. Lim, Caroline Parkin, Shruthi Patel, Basil G. Issa, Anthony J. Maxwell, Julie Wisely, John Belcher, Robert Clarke, Anthony Howell, Emma J. Crosbie, Sacha J. Howell
Summary: This study aims to investigate the cellular and molecular changes in breast and endometrial tissue following total diet replacement (TDR)-induced weight loss in high-risk women, as well as adherence to a 12-month TDR weight loss intervention. The findings of this study are important for understanding the role of TDR in the prevention and management of breast and endometrial cancer.