Article
Medicine, Research & Experimental
Jiasheng Cao, Huijiang Shao, Jiahao Hu, Renan Jin, Anyun Feng, Bin Zhang, Shijie Li, Tianen Chen, Sarun Jeungpanich, Win Topatana, Yitong Tian, Ziyi Lu, Xiujun Cai, Mingyu Chen
Summary: MiR-642a-3p and miR-145-5p were identified as invasion-metastasis associated miRNAs through bioinformatics and experimental validation, and the up-regulation of miR-642a-3p and down-regulation of miR-145-5p may serve as novel treatment options for GBC in the future.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Guifang He, Jing Qiu, Changchang Liu, Ben Tian, Duo Cai, Shihai Liu
Summary: DTYMK is a potential oncogene associated with hepatocellular carcinoma (HCC), and its expression is negatively regulated by miR-148b-3p, which affects HCC cell growth and progression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, General & Internal
Sung Hoon Cho, Young Seok Han, Ja Ryung Han, Hyung Jun Kwon, Seock Hwan Choi, Hyun Tae Kim, Man-Hoon Han, Jae Min Chun
Summary: Gallbladder metastasis of RCC is rare but should be managed cautiously, especially when dealing with polyp changes in patients. Changes in gallbladder polyps in RCC patients should raise suspicions and prompt intervention may lead to better outcomes.
Article
Biochemical Research Methods
Zutan Li, Yuan Zhang, Jingya Fang, Zhihui Xu, Hao Zhang, Minfang Mao, Yuanyuan Chen, Liangyun Zhang, Cong Pian
Summary: Non-coding RNAs, including miRNAs and lncRNAs, play crucial roles in transcriptional processes and regulation of various biological functions. However, existing signaling pathway databases lack information on these non-coding RNAs. In this study, a novel pathway database called NcPath was developed by integrating and visualizing experimentally validated miRNA-target interactions (MTIs) and lncRNA-target interactions (LTIs) across KEGG pathways. Additionally, reliable lncRNA-protein-coding gene (PCG) interaction pairs were identified to expand the usability of the database. Overall, the NcPath database provides valuable annotation and visualization of the involvement of non-coding RNAs in pathways, supporting multimodal enrichment analysis.
Article
Multidisciplinary Sciences
Pinghua Yang, Fengliang Song, Xinwei Yang, Xingzhou Yan, Xinyu Huang, Zhengjun Qiu, Zhijian Wen, Chi Liang, Xianglei Xin, Zhengqin Lei, Kecheng Zhang, Jue Yang, Hu Liu, Hongcheng Wang, Shijun Xiang, Liang Li, Baohua Zhang, Hongyang Wang
Summary: Through biomarker discovery, training, and validation, we have identified a combination of miRNAs that can evaluate the risk of gallbladder carcinoma. This biomarker outperforms standard markers and can be used for early diagnosis. It is also associated with clinicopathological features and prognosis, and regulates cell functions and signaling pathways. This biomarker combination has potential for noninvasive diagnosis, screening, and prognosis prediction for gallbladder carcinoma.
Article
Oncology
Xinyu Liu, Hao Zhang, Pengcheng Zhou, Yaqun Yu, Haoye Zhang, Limin Chen, Jian Gong, Zhenguo Liu
Summary: This study demonstrated that miR-922 regulates malignant behavior in liver cancer cells by targeting ARID2, with the CREB1/miR-922/ARID2 axis potentially serving as a target for liver cancer treatment.
Article
Cell Biology
Honglei Cui, Danfeng Guo, Xiaodan Zhang, Yaohua Zhu, Zhihui Wang, Yang Jin, Wenzhi Guo, Shuijun Zhang
Summary: The study revealed that ENO3 is significantly down-regulated in HCC patients and is related to poor prognosis. Overexpression of ENO3 inhibited the proliferative, migratory, and invasive abilities of HCC cells, while also inhibiting the EMT process. ENO3 further modulated the transcription of genes associated with proliferation and metastasis capacity of HCC cells by suppressing the Wnt/beta-catenin signal.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Dandan Zhou, Ying Wang, Haifeng Hu, Huilin Liu, Jiajia Deng, Lu Li, Chunlei Zheng
Summary: This study found that lncRNA MALAT1 promotes the invasion and metastasis of HCC by regulating the expression of miRNA-613, suggesting its potential as a target for HCC treatment.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Y. Mu, W-J Liu, L-Y Bie, X-Q Mu, Y-Q Zhao
Summary: The study found that VRK2 was significantly increased in tumor tissues and cell lines, and its inhibition promoted apoptosis level and suppressed proliferation, migration, and invasion in A549 cells by regulating the ERK1/2/AKT signaling pathway. Additionally, the luciferase assay revealed that VRK2 could bind to miR-145-5p, and the level of miR-145-5p was negatively correlated with VRK2 expression, playing a role in VRK2 regulation of tumor progression. Knockdown of VRK2 inhibited pulmonary adenocarcinoma development by targeting miR-145-5p and regulating the ERK1/2/AKT signaling pathway.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Min Lin, Jianan Zhang, Hakim Bouamar, Zhiwei Wang, Lu-Zhe Sun, Xueqiong Zhu
Summary: This study reveals that high expression of FBXO22 is associated with clinicopathological characteristics and poor prognosis in cervical cancer patients. Overexpression of FBXO22 promotes cell viability and tumor growth, while knockdown of FBXO22 has opposite effects. Additionally, overexpression of FBXO22 promotes cell cycle progression and inhibits apoptosis in cervical cancer cells. FBXO22 promotes cervical cancer progression by regulating the ubiquitination and proteasomal degradation of the cyclin-dependent kinase inhibitor p57(Kip2).
CELL DEATH & DISEASE
(2022)
Article
Oncology
Yunliang Lu, Xiaohui Zhou, Weilin Zhao, Zhipeng Liao, Bo Li, Peipei Han, Yanping Yang, Xuemin Zhong, Yingxi Mo, Ping Li, Guangwu Huang, Xue Xiao, Zhe Zhang, Xiaoying Zhou
Summary: ACAT1, as a key enzyme in the ketone body metabolism pathway, plays a crucial role in the proliferation and metastasis of nasopharyngeal carcinoma (NPC), potentially acting as a tumor suppressor by regulating epithelial-mesenchymal transition.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Jicheng Duan, Youwen Qian, Xiaohui Fu, Meiling Chen, Kai Liu, Hu Liu, Jiahe Yang, Chen Liu, Yanxin Chang
Summary: The study found that TMEM106C is overexpressed in HCC, and inhibition of TMEM106C can significantly suppress the proliferation and metastasis of HCC. Upregulation of TMEM106C is closely correlated with sex, tumor stage, tumor grade, and other indicators, affecting cell cycle signaling pathways and organelle fission through the regulation of CDK kinases.
Article
Oncology
Vaishali Jain, Javed Akhtar, Ratna Priya, Puja Sakhuja, Surbhi Goyal, Anil Kumar Agarwal, Vivek Ghose, Ravindra Varma Polisetty, Ravi Sirdeshmukh, Fouzia Siraj, Poonam Gautam
Summary: This study identified proteins associated with lymph node metastasis in gallbladder cancer, which may be further explored for diagnostics and therapeutics in lymph node positive cases.
Article
Oncology
Yuan Yuan Gu, Guan Nan Zhou, Yao Li, Hong Yu He, Jing Xin Ding, Ke Qin Hua
Summary: The study reveals that HDAC10 inhibits the invasiveness and tumorigenesis of cervical cancer by downregulating miR-223 and targeting EPB41L3 expression. This finding provides a new insight for the management of cervical cancer and precancer lesions.
JOURNAL OF ONCOLOGY
(2022)
Article
Oncology
Xin Zhang, Hongwei Zhang, Zhibin Liao, Jiacheng Zhang, Huifang Liang, Weixing Wang, Jia Yu, Keshuai Dong
Summary: This study reveals that SHC4 activates STAT3 signaling to promote hepatocellular carcinoma (HCC) progression. SHC4 overexpression in HCC is associated with poor prognosis, increased migration and invasion. SHC4 enhances cell proliferation, colony formation, migration, and invasion, as well as cell cycle progression and epithelial-mesenchymal transition (EMT) in HCC cells. Additionally, the activation of STAT3 signaling is observed in SHC4 overexpressed HCC cells and tissues. Moreover, intervention of STAT3 confirms its importance in the oncogenic role of SHC4 in HCC.
CANCER CELL INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.