Article
Virology
Chun-Chiao Yu, Pei-Chen Lin, Cho-Han Chiang, Shu-Tang Jen, Yen-Ling Lai, Shih-Chin Hsu, Lee-Chiang Lo, Jing-Jer Lin, Nei-Li Chan, Ming-Jiun Yu
Summary: In this study, we discovered for the first time that the ATP-binding domain of NS3 is involved in NS5A phosphorylation. In vitro assays showed that casein kinase I alpha is a very potent kinase responsible for NS5A phosphorylation at serines 225, 232, and 235. Our data suggest that ATP binding by NS3 probably results in conformational changes that recruit casein kinase I alpha to phosphorylate NS5A, initially at S225 and subsequently at S232 and S235. Our discovery reveals intricate requirements of the structural integrity of NS3 for NS5A hyperphosphorylation and HCV replication.
JOURNAL OF VIROLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Yu-Ying Chen, Thomas V. O'Halloran
Summary: Zinc is crucial for living organisms, and it has been discovered that the ZNG1 family of GTPases function as metallochaperones, directly transferring zinc to and activating methionine aminopeptidases which play essential roles in protein modification during or after translation.
Article
Biochemistry & Molecular Biology
Nicolas Bloemeke, Kevin Meighen-Berger, Manuel Hitzenberger, Nina C. Bach, Marina Parr, Joao Pl Coelho, Dmitrij Frishman, Martin Zacharias, Stephan A. Sieber, Matthias J. Feige
Summary: One-third of the human proteome is composed of membrane proteins, which are susceptible to misfolding and require folding assistance from chaperones. Calnexin, an abundant ER chaperone, interacts with a large number of membrane proteins, including misfolded ones, using its lectin domain and transmembrane domain for recognition and binding. This study reveals the widespread role of calnexin in recognizing client proteins in the lipid bilayer and highlights its importance in supporting membrane protein biogenesis.
Review
Veterinary Sciences
Tong Zhou, Mingshu Wang, Anchun Cheng, Qiao Yang, Bin Tian, Ying Wu, Renyong Jia, Shun Chen, Mafeng Liu, Xin-Xin Zhao, Xuming Ou, Sai Mao, Di Sun, Shaqiu Zhang, Dekang Zhu, Juan Huang, Qun Gao, Yanling Yu, Ling Zhang
Summary: This article mainly describes the viral protein kinases and their mechanisms of regulating viral protein function through phosphorylation. The study of post-translational modification of viral proteins is of great significance for understanding viral infection mechanisms and developing antiviral treatment.
VETERINARY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hyunju Cho, Woo Jun Shim, Yumeng Liu, Shu-ou Shan
Summary: J-domain proteins (JDPs) play crucial roles in assisting Hsp70 in client trapping and regulating the Hsp70 ATPase cycle. In this study, it was found that two JDPs, Ydj1 and Sis1, work in parallel to support the targeting of tail-anchored proteins (TAs) to the endoplasmic reticulum (ER). Biochemical analyses showed that both Ydj1 and Sis1 enhance the transfer of Hsp70-bound TAs to Sgt2, ultimately supporting TA insertion in vivo.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Paolo Costa, Giulia Usai, Angela Re, Marcello Manfredi, Giuseppe Mannino, Cinzia Margherita Bertea, Enrica Pessione, Roberto Mazzoli
Summary: Comparative gel-free proteomics analysis of Clostridium cellulovorans exposed to butanol revealed modulation of protein biosynthesis, up-regulation of ATPases, and rearrangement of cell envelope, suggesting potential gene targets for metabolic engineering strategies aimed at improving butanol tolerance in this microbial strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Chemistry, Physical
Marcela Slovakova, Zuzana Bilkova
Summary: Phosphorylation is a key posttranslational process that affects protein function, and understanding it is crucial for studying disease pathogenesis and designing new therapeutic strategies. Affordable kinases with varying activity, specificity, and stability offer opportunities for in vitro research and modulation of phosphorylation reactions, facilitating the production of active recombinant proteins.
Article
Multidisciplinary Sciences
Maximilian M. Biebl, Abraham Lopez, Alexandra Rehn, Lee Freiburger, Jannis Lawatscheck, Birgit Blank, Michael Sattler, Johannes Buchner
Summary: The study shows that a tryptophan residue in the proximal region of the p23 tail decelerates the ATPase of Hsp90 by altering the conformation of the catalytic loop, while a conserved helical motif in the p23 tail interacts with the client protein binding site of Hsp90 and is involved in the activation of the client protein in the cellular context.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Xiaochuan Liu, Yen-Yu Yang, Yinsheng Wang
Summary: This study revealed a novel mechanism of HSP90 and Aha1 in regulating the miRNA pathway through promoting the folding of Dicer1 protein and demonstrated that Aha1 modulates this process by acting as an autonomous chaperone and a co-chaperone for HSP90.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Dimitra Keramisanou, M. V. Vasantha Kumar, Nicole Boose, Rinat R. Abzalimov, Ioannis Gelis
Summary: This study investigates the recruitment and loading mechanism of protein kinases to the Hsp90-Cdc37 complex, which is the first step in Hsp90-mediated chaperoning. The results show that the conformational dynamics of all partners are critical for the loading mechanism, and that Cdc37 plays a role in sensing clients by stabilizing the preexisting partially unfolded client state. These findings reveal the regulatory mechanism of molecular chaperones in maintaining protein homeostasis.
Editorial Material
Hematology
Monika Haemmerle
Summary: This study explores the role of Mnk1 in megakaryocytes and platelets, showing that it regulates protein synthesis and affects megakaryocyte ploidy and platelet production. These findings have clinical implications and contribute to our understanding of platelet function and thrombosis.
Article
Biochemistry & Molecular Biology
Kanghyun Lee, Aye C. Thwin, Cory M. Nadel, Eric Tse, Stephanie N. Gates, Jason E. Gestwicki, Daniel R. Southworth
Summary: The Hsp90 chaperone facilitates the folding and activation of hundreds of client proteins in the cell, with FKBP51 serving as a catalytic co-chaperone during the folding process. The cryo-EM structure of the human Hsp90:FKBP51:p23 complex revealed the key interactions between cochaperones and Hsp90 during client maturation.
Review
Biochemistry & Molecular Biology
Chrisostomos Prodromou, Dennis M. Bjorklund
Summary: Hsp90, an ATP molecular chaperone, plays a crucial role in the activation and maturation of client proteins. Recent studies have shed light on the mechanism of Hsp90, including its ATP hydrolysis and the remodeling of client proteins for their activation.
Article
Multidisciplinary Sciences
Ryan M. Bitter, SeCheol Oh, Zengqin Deng, Suhaila Rahman, Richard K. Hite, Peng Yuan
Summary: ATP7A and ATP7B are two homologous copper-transporting P1B-type ATPases that play important roles in cellular copper homeostasis. This study reveals the structure of frog ATP7B in a copper-free state and shows that the metal-binding domains interact with both the A and P domains. Furthermore, a network of copper-coordinating ligands in the membrane delineates a stepwise copper transport pathway.
Article
Virology
Lamya Alomair, Sabeena Mustafa, Mohsin Saleet Jafri, Wardah Alharbi, Abdulrhman Aljouie, Fahad Almsned, Mohammed Alawad, Yahya Abdulfattah Bokhari, Mamoon Rashid
Summary: This study used neural networks and molecular dynamics simulations to predict potential kinases for SARS-CoV-2 phosphorylation and investigate its effects on protein structure. Eight phosphorylation sites were identified as important for viral replication, and specific residues were found to be affected by phosphorylation, impacting protein function and stability.
Article
Chemistry, Multidisciplinary
Axel Pahl, Markus Lakemeyer, Marie-Theres Vielberg, Mathias W. Hackl, Jan Vomacka, Vadim S. Korotkov, Martin L. Stein, Christian Fetzer, Katrin Lorenz-Baath, Klaus Richter, Herbert Waldmann, Michael Groll, Stephan A. Sieber
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2015)
Article
Chemistry, Multidisciplinary
Franziska A. Mandl, Volker C. Kirsch, Ilke Ugur, Elena Kunold, Jan Vomacka, Christian Fetzer, Sabine Schneider, Klaus Richter, Thilo M. Fuchs, Iris Antes, Stephan A. Sieber
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Biochemistry & Molecular Biology
Julia M. Eckl, Marina Daake, Sebastian Schwartz, Klaus Richter
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Alexandra Rehn, Elisabetta Moroni, Bettina K. Zierer, Franziska Tippel, Giulia Morra, Christine John, Klaus Richter, Giorgio Colombo, Johannes Buchner
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Bettina K. Zierer, Martin Ruebbelke, Franziska Tippel, Tobias Madl, Florian H. Schopf, Daniel A. Rutz, Klaus Richter, Michael Sattler, Johannes Buchner
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Zeinab Anvarian, Hisashi Nojima, Eline C. van Kappel, Tobias Madl, Maureen Spit, Martin Viertler, Ingrid Jordens, Teck Y. Low, Revina C. van Scherpenzeel, Ineke Kuper, Klaus Richter, Albert J. R. Heck, Rolf Boelens, Jean-Paul Vincent, Stefan G. D. Rudiger, Madelon M. Maurice
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Bettina K. Zierer, Martin Ruebbelke, Franziska Tippel, Tobias Madl, Florian H. Schopf, Daniel A. Rutz, Klaus Richter, Michael Sattler, Johannes Buchner
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Zeinab Anvarian, Hisashi Nojima, Eline C. van Kappel, Tobias Madl, Maureen Spit, Martin Viertler, Ingrid Jordens, Teck Y. Low, Revina C. van Scherpenzeel, Ineke Kuper, Klaus Richter, Albert J. R. Heck, Rolf Boelens, Jean-Paul Vincent, Stefan G. D. Rudiger, Madelon M. Maurice
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Julia Eckl, Siyuan Sima, Katrin Marcus, Claudia Lindemann, Klaus Richter
Article
Multidisciplinary Sciences
Bastian Braeuning, Eva Bertosin, Florian Praetorius, Christian Ihling, Alexandra Schatt, Agnes Adler, Klaus Richter, Andrea Sinz, Hendrik Dietz, Michael Groll
NATURE COMMUNICATIONS
(2018)
Article
Chemistry, Multidisciplinary
Matthias Stahl, Vadim S. Korotkov, Dora Balogh, Leonhard M. Kick, Malte Gersch, Axel Pahl, Pavel Kielkowski, Klaus Richter, Sabine Schneider, Stephan A. Sieber
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Biochemistry & Molecular Biology
Rasmus Ree, Laura Kind, Anna Kaziales, Sylvia Varland, Minglu Dai, Klaus Richter, Adrian Drazic, Thomas Arnesen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Cell Biology
Siyuan Sima, Lukas Schmauder, Klaus Richter
Article
Cell Biology
Katharina Papsdorf, Siyuan Sima, Gerhard Richter, Klaus Richter
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)