Article
Materials Science, Multidisciplinary
Tun Naw Sut, Bo Kyeong Yoon, Won-Yong Jeon, Joshua A. Jackman, Nam-Joon Cho
Summary: Supported lipid bilayer (SLB) coatings, versatile cell-membrane-mimicking biointerfaces, have been expanded for medical and biotechnology applications, facilitating various studies and applications like biosensors, diagnostics, and antifouling surfaces.
APPLIED MATERIALS TODAY
(2021)
Article
Chemistry, Physical
Asma El Aoud, Emmanuelle Reboul, Aurelien Dupont, Cristelle Meriadec, Franck Artzner, Sebastien Marze
Summary: This study investigated structural transitions in intestinal assemblies containing bile components, dietary fat, and fat-soluble vitamins. Results showed that increasing dietary lipid concentration led to a transition from core-shell micelles to unilamellar vesicles, while increasing fat-soluble vitamin concentration induced various structural transitions in the assemblies. SAXS data was used to propose ideal molecular organizations for the assemblies with and without alpha-tocopherol.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Nicolo Paracini, Philipp Gutfreund, Rebecca Welbourn, Juan Francisco Gonzalez-Martinez, Kexin Zhu, Yansong Miao, Nageshwar Yepuri, Tamim A. . Darwish, Christopher Garvey, Sarah Waldie, Johan Larsson, Max Wolff, Marite Cardenas
Summary: Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We investigated the structure of lipid bilayers formed on nanoparticle arrays through vesicle fusion using scattering techniques and direct imaging. The presence of both flat and curved supported lipid bilayers on the same substrate, combined with the accuracy of grazing incidence neutron scattering, offers a promising approach to studying curvature-dependent membrane phenomena.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Chemistry, Multidisciplinary
Aoon Rizvi, Justin T. Mulvey, Joseph P. Patterson
Summary: The study investigates the formation of block-copolymer-supported bilayers using LP-TEM, revealing two formation pathways involving liquid droplets or vesicles as intermediates. The results suggest that bilayer assembly methods via liquid droplet intermediates are beneficial for forming pristine supported bilayers, and supported bilayers inside liquid cells may be useful for imaging membrane interactions with proteins and nanoparticles in the future.
Article
Biochemistry & Molecular Biology
Kathleen W. Swana, Terri A. Camesano, Ramanathan Nagarajan
Summary: Supported lipid bilayers (SLBs) on quartz crystals are versatile model systems for studying cell membrane behavior. This study successfully formed entirely anionic SLBs by treating the quartz crystal, which provides valuable model systems for studying interactions with Gram-positive bacterial membranes.
Article
Chemistry, Physical
Mariia Savenko, Timothee Rivel, Semen Yesylevskyy, Christophe Ramseyer
Summary: The study found that supported lipid bilayers exhibit close-contact conformations on hydrophilic substrates, while they form self-assembled monolayers on hydrophobic substrates. Lipids and water at the surface undergo redistribution to maintain the stability of the bilayers on hydrophilic substrates, and the bilayer thickness and water molecules per lipid in the hydration layer change with hydrophilicity.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Multidisciplinary Sciences
Christina M. Bailey-Hytholt, Veronica LaMastro, Anita Shukla
Summary: Model cell membranes serve as a valuable tool for drug discovery and toxicity studies, with lipid bilayers mimicking cell membranes to study molecular interactions. Methods involve lipid extraction from primary cells or cell lines followed by mass spectrometry to fabricate lipid vesicles with specific lipid compositions for supported and suspended lipid bilayers. These models allow for the rapid assessment of interaction mechanisms between molecules and cell membranes, aiding in drug candidate screening and toxicity prediction.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Nanoscience & Nanotechnology
Hyeong Jin Kim, Wenjie Wang, Wei Bu, Surya K. Mallapragada, David Vaknin
Summary: An assembly of silver nanoparticles grafted with thiolated poly(ethylene glycol) is investigated using synchrotron-based X-ray surface-sensitive diffraction methods and small-angle X-ray scattering. The addition of K2CO3 or polyacrylic acid induces different structures in the assemblies. The core type of nanoparticles plays a vital role in controlling the structures, suggesting potential applications in plasmonics and photonics.
ACS APPLIED NANO MATERIALS
(2022)
Article
Biology
Zhe Feng, Mingjie Zhang
Summary: Eukaryotic cells use compartmentalization to regulate biological functions. Non-or semi-membrane-bound compartments can form biological condensates through liquid-liquid phase separation. Biochemical reconstitution techniques are important for understanding the organization of these condensates, but may not fully capture the physiological configuration of compartments attached to the plasma membrane. In this study, a minimal system was used to demonstrate biochemical reconstitution on a supported lipid bilayer, mimicking the organization of molecular components in postsynaptic termini.
Article
Nanoscience & Nanotechnology
Zachary A. Manzer, Surajit Ghosh, Miranda L. Jacobs, Srinivasan Krishnan, Warren R. Zipfel, Miguel Pineros, Neha P. Kamat, Susan Daniel
Summary: This study introduces a new approach to integrate membrane proteins into hybrid-supported lipid bilayers using a cell-free expression system, allowing for the interface between cellular mimetic structures and analytical tools. Different methods are demonstrated to successfully integrate proteins into hybrid-supported lipid bilayers, leading to oriented protein assembly, with diblock copolymers facilitating protein mobility within the bilayers.
ACS APPLIED BIO MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Jonas Schaefer, Jessica Nehls, Markus Schoen, Ingo Mey, Claudia Steinem
Article
Multidisciplinary Sciences
Ilona Kondratiuk, Shrutee Jakhanwal, Jialin Jin, Udhayabhaskar Sathyanarayanan, Benjamin Kroppen, Ajaybabu Pobbati, Anita Krisko, Uri Ashery, Michael Meinecke, Reinhard Jahn, Dirk Fasshauer, Ira Milosevic
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biophysics
Peter Muehlenbrock, Kira Herwig, Loan Vuong, Ingo Mey, Claudia Steinem
BIOPHYSICAL JOURNAL
(2020)
Article
Multidisciplinary Sciences
Thomas Schubert, Taras Sych, Josef Madl, Maokai Xu, Ramin Omidvar, Lukas J. Patalag, Annika Ries, Katharina Kettelhoit, Annette Brandel, Yves Mely, Claudia Steinem, Daniel B. Werz, Roland Thuenauer, Winfried Roemer
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Xiandeng Wu, Marcelo Ganzella, Jinchuan Zhou, Shihan Zhu, Reinhard Jahn, Mingjie Zhang
Summary: The tethering of synaptic vesicles to the active zone is determined by phase separation, forming two distinct SUV pools, which have general implications in cellular processes like vesicular formation and trafficking.
Article
Cell Biology
Cyril Cheret, Marcelo Ganzella, Julia Preobraschenski, Reinhard Jahn, Gudrun Ahnert-Hilger
Summary: VGLUTs play a dual role in neurons by filling synaptic vesicles with glutamate and regulating intracellular Pi levels through transport activity. Studies have shown that VGLUT-mediated Pi influx is counteracted by Pi efflux, highlighting the importance of VGLUTs in both glutamate loading and Pi restoration.
Article
Biochemical Research Methods
Ivan Silbern, Kuan-Ting Pan, Maksims Fiosins, Stefan Bonn, Silvio O. Rizzoli, Eugenio F. Fornasiero, Henning Urlaub, Reinhard Jahn
Summary: The study investigates the impact of synaptic vesicle cycling-dependent phosphorylation events on various functions in synaptic transmission, including exo- and endocytosis processes.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Sabine Wittig, Marcelo Ganzella, Marie Barth, Susann Kostmann, Dietmar Riedel, Angel Perez-Lara, Reinhard Jahn, Carla Schmidt
Summary: The protein interactions within synaptic vesicles are complex and can be categorized into specific modules or interactions caused by 'crowding'. Understanding these interactions provides insights into the composition and function of synaptic vesicles.
NATURE COMMUNICATIONS
(2021)
Review
Biophysics
Tobias Grothe, Julia Nowak, Reinhard Jahn, Peter Jomo Walla
Summary: The passage describes various fluorescence-based methods developed over the past decade to monitor membrane fusion, docking, distances, and curvature. These tools were primarily developed using liposomes as model systems, and their application range and limitations are discussed with examples from protein-mediated vesicle docking and fusion studies.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Judyta K. Juranek, Konark Mukherjee, Reinhard Jahn, Jia-Yi Li
Summary: Synaptic transmission relies on neurotransmitters stored in synaptic vesicles and released at the active zone. Research shows a robust bi-directional, coordinated trafficking of synaptic vesicle and active zone proteins in peripheral nerves, which plays a critical role in neuronal function and neurodegenerative diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Agata Witkowska, Leonard P. Heinz, Helmut Grubmueller, Reinhard Jahn
Summary: Proteins driving membrane fusion must overcome energy barriers to induce intermediate steps where lipid bilayers' integrity is lost. The metastable intermediate preceding hemifusion does not require but is enhanced by divalent cations, characterized by the absence of proteins and local membrane thickening, induced by lipid rearrangements due to dehydration of the membrane surface revealed by simulations.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Changwon Kim, Min Ju Shon, Sung Hyun Kim, Gee Sung Eun, Je-Kyung Ryu, Changbong Hyeon, Reinhard Jahn, Tae-Young Yoon
Summary: Using single-molecule methods, researchers demonstrated the cooperativity between NSF and the SNARE complex to prevent ATP consumption without productive disassembly.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Seiichi Koike, Reinhard Jahn
Summary: Membrane traffic in eukaryotic cells is regulated by transport vesicles that contain targeting signals recognized by tethering factors. The final step, membrane fusion, is mediated by SNARE proteins. The role of SNARE proteins in targeting specificity is still a topic of debate.
BIOCHEMICAL JOURNAL
(2022)
Review
Cell Biology
Reinhard Jahn, David C. Cafiso, Lukas K. Tamm
Summary: This article reviews the SNARE-mediated fusion pathway, including the assembly of SNARE proteins into complexes, the regulation of this assembly by accessory proteins, and the overcoming of energy barriers that prevent membrane fusion.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Chemistry, Physical
Jeremias Sibold, Vera E. Tewaag, Thomas Vagedes, Ingo Mey, Claudia Steinem
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2020)
Article
Biochemistry & Molecular Biology
Philipp Grad, Katarina Edwards, Lars Gedda, Victor Agmo Hernandez
Summary: This study investigates the effects of polyethylene glycol-(PEG) modified lipids and gangliosides on the Ca2+ induced interaction between liposomes composed of palmitoyl-oleoyl phosphatidylethanolamine (POPE) and palmitoyl-oleoyl phosphatidylserine (POPS) at physiological ionic strength. The results show that naked liposomes tend to adhere, rupture, and collapse on each other's surfaces upon addition of Ca2+, eventually resulting in the formation of large multilamellar aggregates and bilayer sheets. However, the presence of gangliosides or PEGylated lipids leads to the formation of small, long-lived bilayer fragments/disks. PEGylated lipids seem to be more effective than gangliosides at stabilizing these structures. The study suggests that direct liposome-liposome fusion is not the dominating process triggered by Ca2+ in the systems studied.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Saurabh Saraswat, Archana Chugh
Summary: Cell penetrating peptides (CPP) are important tools for intracellular delivery of molecules. In this study, a novel marine-derived CPP from Engraulis japonicus, called Engraulisin, was reported. Engraulisin demonstrated successful cellular uptake and selective antimicrobial activity against Methicillin-resistant Staphylococcus aureus (MRSA), making it a potential candidate for drug delivery.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Emanuela Efodili, Ashlynn Knight, Maryem Mirza, Cedric Briones, Il-Hyung Lee
Summary: This study demonstrates the spontaneous transfer of small membrane-bound peptides between a supported lipid bilayer and giant unilamellar vesicles in vitro. The transfer occurs through the formation of hemi-fusion stalks and is limited to small peptides due to the highly curved structure of the stalk. This system provides a synthetic platform for studying peptide trafficking between synthetic membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Niki Baccile, Vincent Chaleix, Ingo Hoffmann
Summary: This study measured the bending rigidity of self-assembled structures formed by a new biobased glucolipid bioamphiphile using neutron spin-echo (NSE) technology. The results showed that the bending rigidity of these structures is lower or higher compared to phospholipid membranes, providing a new theoretical basis for the application of these new molecular systems.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Alain Bolano Alvares, Pablo E. A. Rodriguez, Gerardo D. Fidelio
Summary: The behavior of amphiphilic molecules at the air/water interface was studied to evaluate the arrangement formed in a confined area. The surface properties of zwitterionic DPPC lipid and A beta(1-40) amyloid peptide in mixed films were investigated at different temperatures. DPPC undergoes a phase transition depending on temperature and lateral pressure, which allows for the study of its influence on amyloid structure formation.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Lukasz Plachta, Marzena Mach, Magdalena Kowalska, Pawel Wydro
Summary: Resveratrol, a popular phytoalexin found in grapes and red wine, has beneficial effects on the cardiovascular system, exhibits antiviral, antibacterial, and antifungal properties, and may have therapeutic effects against cancer. This study investigates the interaction of resveratrol with model cell membranes, demonstrating changes in their physicochemical parameters and highlighting the role of cholesterol content in resveratrol incorporation. The findings suggest that the molecular mechanism of action of resveratrol may involve interactions with lipid rafts.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Edurne Rujas, Beatriz Apellaniz, Johana Torralba, David Andreu, Jose M. M. Caaveiro, Shixia Wang, Shan Lu, Jose L. Nieva
Summary: This study investigates the role of fusion peptide and Trp-rich membrane proximal external region in HIV-1 fusion and demonstrates that liposome-based formulations containing FP-MPER hybrid peptides can induce the production of specific neutralizing antibodies in rabbits. The results support the use of liposomes as vaccine carriers and the inclusion of lipid membranes in immunogens to elicit specific humoral responses.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Dailin Li, Dingyuan Shi, Lei Wang
Summary: G protein-gated inwardly rectifying potassium (GIRK) channels play a significant role in regulating cell excitability. This study investigated the ion permeation mechanism in GIRK2 mutants using molecular dynamic simulations and QM/MM methods. The results revealed the importance of a multi-ion distribution for ion conduction.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick Allen, Adam C. Smith, Vernon Benedicto, Abbas Abdulhasan, Vasanthy Narayanaswami, Enrico Tapavicza
Summary: We explore the stability, size, and structure of nanodiscs formed by the N-terminal domain of apolipoprotein E3 and a variable number of 1,2-dimyristoyl-sn-glycero-3-phosphocholine molecules. Our simulations suggest that nanodiscs containing 240 to 420 DMPC molecules are stable. The antiparallel configuration shows more protein-protein interactions and ionic contacts, as well as greater stability and rigidity compared to the parallel configuration.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Patrick M. Keating, Nicholas P. Schifano, Xinrui Wei, Matthew Y. Kong, Jinwoo Lee
Summary: Lassa virus (LASV) infection is dependent on the fusion of its viral membrane with the host cell membrane, and our study reveals that this fusion process involves pH-dependent conformational changes in the transmembrane domain of LASV. These findings provide valuable insights into the fusion mechanism of LASV and can be utilized in the design of therapeutics to combat Lassa virus infections and prevent its potential spread.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Ashley L. Bennett, Kristen N. Cranford, Austin L. Bates, Christopher R. Sabatini, Hee-Seung Lee
Summary: This study employed molecular dynamics simulations to elucidate the binding, folding, and insertion details of TP10W in the cell membrane. The simulations revealed that TP10W can translocate across the lipid membrane as a monomer, and the charged amino acid side chains can move between lipid leaflets. Comparison with point mutated variants demonstrated significant conformational changes due to charge distribution.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemistry & Molecular Biology
Jessica H. van Wonderen, Jason C. Crack, Marcus J. Edwards, Thomas A. Clarke, Gerhard Saalbach, Carlo Martins, Julea N. Butt
Summary: Electrogenic bacteria transfer excess respiratory electrons to metal oxide particles and electrodes through the MtrCAB complex. The crystal structure of MtrCAB from S. baltica OS185 was resolved, and liquid chromatography mass spectrometry was used to determine the mass values of the three proteins in purified MtrCAB complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Review
Biochemistry & Molecular Biology
Yi-Ming Chen, Ching-Tai Lu, Chia-Wen Wang, Wolfgang B. Fischer
Summary: A series of dye ligands were ranked based on docking experiments, selecting the most suitable ligands for binding with different proteins. Additionally, a comparison study was conducted on a series of repurposing drugs and known antivirals.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
