Article
Immunology
Hannah-Lena Obermann, Ines I. Lederbogen, Jenny Steele, Jens Dorna, Leif Erik Sander, Konrad Engelhardt, Udo Bakowsky, Andreas Kaufmann, Stefan Bauer
Summary: The innate immune system detects viral and bacterial RNA through pattern recognition receptors, activating the immune system. Cholesterol-conjugated RNA forms nanoparticles that enhance RNA uptake and have strong immune-stimulating effects, simplifying the use of immunostimulatory RNA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
William D. Jackson, Chiara Giacomassi, Sophie Ward, Amber Owen, Tiago C. Luis, Sarah Spear, Kevin J. Woollard, Cecilia Johansson, Jessica Strid, Marina Botto
Summary: This study demonstrates that repetitive TLR7 stimulation can induce an emergency bone marrow monocyte response, independent of the canonical CCR2 and CX3CR1 axes or prototypical cytokines. The monocytes released from the bone marrow differentiate into monocytes and macrophages in multiple organs, and display a reduced cytokine response to further TLR7 stimulation, leading to reduced lung viral load after RSV and influenza virus infection.
Article
Immunology
Natalya Odoardi, Olena Kourko, Carlene Petes, Sameh Basta, Katrina Gee
Summary: Regulation of proinflammatory cytokine expression is crucial in ssRNA virus infections. IL-27 has a modulatory role on TLR7 and TLR8 responses, enhancing TLR7 expression and TLR8-mediated cytokine secretion. Additionally, TLR7 agonist can inhibit cytokine and chemokine production induced by a TLR8 agonist.
JOURNAL OF INNATE IMMUNITY
(2021)
Article
Oncology
Dmitry Namgaladze, Bernhard Bruene
Summary: Pharmacological activation of tumor suppressor p53 demonstrates anti-inflammatory effects in differentiating human macrophages.
Article
Immunology
Takuma Shibata, Ryota Sato, Masato Taoka, Shin-Ichiroh Saitoh, Mayumi Komine, Kiyoshi Yamaguchi, Susumu Goyama, Yuji Motoi, Jiro Kitaura, Kumi Izawa, Yoshio Yamauchi, Yumiko Tsukamoto, Takeshi Ichinohe, Etsuko Fujita, Ryosuke Hiranuma, Ryutaro Fukui, Yoichi Furukawa, Toshio Kitamura, Toshiyuki Takai, Arinobu Tojo, Mamitaro Ohtsuki, Umeharu Ohto, Toshiyuki Shimizu, Manabu Ozawa, Nobuaki Yoshida, Toshiaki Isobe, Eicke Latz, Kojiro Mukai, Tomohiko Taguchi, Hiroaki Hemmi, Shizuo Akira, Kensuke Miyake
Summary: Loss-of-function mutations in SLC29A3 cause histiocytic diseases, which are driven by TLR7 and TLR8, endosomal sensors for nucleosides and oligoribonucleotides. TLR7 promotes the proliferation and maturation of monocytes, leading to the accumulation of phagocytes and histiocytosis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Megha Srinivas, Preeti Sharma, Siddharth Jhunjhunwala
Summary: Research findings suggest that immune cells can phagocytose particles under constant fluidic flow. Different cell types show similar or better uptake rates under flow conditions. Even in the presence of crowded red blood cells, neutrophils and monocytes are able to uptake particles while flowing.
MOLECULAR PHARMACEUTICS
(2021)
Article
Chemistry, Medicinal
Samo Guzelj, Matjaz Weiss, Bram Slutter, Ruza Frkanec, Ziga Jakopin
Summary: This study utilizes the synergistic cross-talk between NOD2 and TLR7 to design two conjugated agonists that exhibit potent immunostimulatory activities in human and murine cells, generating a strong antigen-specific immune response in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Immunology
Emily Fraser, Laura Denney, Agne Antanaviciute, Karl Blirando, Chaitanya Vuppusetty, Yuejuan Zheng, Emmanouela Repapi, Valentina Iotchkova, Stephen Taylor, Neil Ashley, Victoria St Noble, Rachel Benamore, Rachel Hoyles, Colin Clelland, Joseph M. D. Rastrick, Clare S. Hardman, Nasullah K. Alham, Rachel E. Rigby, Alison Simmons, Jan Rehwinkel, Ling-Pei Ho
Summary: Monocytes in idiopathic pulmonary fibrosis (IPF) patients exhibit a distinct immunophenotype from age-matched controls, with an activated type I interferon pathway that may contribute to driving chronic inflammation and fibrosis. These findings strengthen the potential role of monocytes in the pathogenesis of IPF.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Aida Dama, Chiara Baggio, Carlotta Boscaro, Mattia Albiero, Andrea Cignarella
Summary: Estrogen receptor activity plays a crucial role in mediating various physiological processes in the cardiovascular system. Gender and hormonal factors have significant impacts on endothelial biology, particularly in cardiometabolic risk post-menopause. Additionally, estrogen pathways regulate angiogenesis through complex mechanisms and influence other cell types like monocytes and macrophages, offering potential therapeutic solutions for cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Martina Fejtkova, Martina Sukova, Katerina Hlozkova, Karolina Skvarova Kramarzova, Marketa Rackova, David Jakubec, Marina Bakardjieva, Marketa Bloomfield, Adam Klocperk, Zuzana Parackova, Anna Sediva, Jahnavi Aluri, Michaela Novakova, Tomas Kalina, Eva Fronkova, Ondrej Hrusak, Hana Malcova, Petr Sedlacek, Zuzana Liba, Martin Kudr, Jan Stary, Megan A. Cooper, Michael Svaton, Veronika Kanderova
Summary: Our study identified a novel mutation c.1715G>T (p.G572V) in the Toll-like receptor 8 (TLR8) gene, which causes an autoimmune and autoinflammatory disorder in a family with monozygotic male twins. In vitro assays confirmed the pathogenicity of the mutation and revealed increased inflammatory responses.
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yuling Chen, Timo Gaber
Summary: Oxygen availability varies in the human body, impacting immune cell function through transcriptional regulation by HIFs. Severe hypoxia under pathophysiological conditions can lead to dysfunctional immune response and tissue damage.
Article
Pharmacology & Pharmacy
Gabriela Hrckova, Terezia Macak Kubaskova, Dagmar Mudronova, Zuzana Jurcackova, Denisa Ciglanova
Summary: The combination of human leukocyte extract (DLE) and anthelmintic albendazole (ABZ) enhances the effects of anthelmintic treatment and alleviates the immunosuppressive response caused by infection. This study provides insights into the significant role of DLE and ABZ combination therapy in modulating immune response.
Article
Multidisciplinary Sciences
Marc A. Fernandez-Yague, Lauren A. Hymel, Claire E. Olingy, Claire McClain, Molly E. Ogle, Jose R. Garcia, Dustin Minshew, Sofiya Vyshnya, Hong Seo Lim, Peng Qiu, Andres J. Garcia, Edward A. Botchwey
Summary: Understanding the immune response to hydrogel implantation is crucial for designing immunomodulatory biomaterials. Through the use of advanced techniques such as high-dimensional flow cytometry and intravital imaging, the study found that RGD-presenting hydrogels can promote cellular infiltration and revascularization, and can direct the immune response through controlling macrophage cytokine secretion.
Article
Neurosciences
Ruthe Storgaard Dieu, Vian Wais, Michael Zaucha Sorensen, Joanna Marczynska, Magdalena Dubik, Stephanie Kavan, Mads Thomassen, Mark Burton, Torben Kruse, Reza Khorooshi, Trevor Owens
Summary: Studies have shown that modulation of innate receptors TLR7 and TLR9 signaling in the central nervous system can induce different inflammatory responses, with potential therapeutic implications for diseases such as multiple sclerosis.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Immunology
Longhuan Ma, Morgan Terrell, Josephine Brown, Abigail Castellanos Garcia, Ahmed Elshikha, Laurence Morel
Summary: TLR7/8 activation induces leaky gut in lupus-prone mice mediated by adaptive immune responses, but it does not breach gut barrier integrity in non-autoimmune mice.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)