Article
Cell Biology
Min Soo Kim, Da-Hyun Kim, Hyun Kyoung Kang, Myung Geun Kook, Soon Won Choi, Kyung-Sun Kang
Summary: Novel brain organoid model systems have been developed from adult dermal fibroblast-derived neural stem cells, exhibiting characteristics of the human cerebral cortex for studying neuronal proliferation and maturation. When subjected to hypoxic injury, reoxygenation restored neuronal cell proliferation but not maturation in these neural organoids, suggesting new opportunities for drug screening and personalized modeling of neurodegenerative diseases.
Review
Biochemistry & Molecular Biology
Kseniia Sarieva, Simone Mayer
Summary: The translation provides an overview of how prenatal environmental adversity impacts human brain development and the use of brain organoids to study these effects. It also highlights the challenges in the field, such as protocol variability and a lack of benchmarking for cross-study comparisons.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemical Research Methods
Na Young Choi, Moo-Yeal Lee, Sehoon Jeong
Summary: Neurological diseases are caused by defects in the brain, spinal cord, or nervous system. Ex vivo brain tissues and brain organoids have been used to model these diseases, while the blood-brain barrier plays a crucial role in regulating brain homeostasis. However, the blood-brain barrier can also hinder the penetration of therapeutic drugs, leading to neurological diseases. Stem cells and brain organoid-on-a-chip systems have been developed to study and overcome these challenges in neurological disease research.
Article
Biochemistry & Molecular Biology
Bumsoo Kim, Yongjun Koh, Hyunsu Do, Younghee Ju, Jong Bin Choi, Gahyang Cho, Han-Wook Yoo, Beom Hee Lee, Jinju Han, Jong-Eun Park, Yong-Mahn Han
Summary: This study reveals that Noonan syndrome (NS) patients exhibit developmental abnormalities in cortical organoids (NS-COs) derived from induced pluripotent stem cells (iPSCs) due to gain-of-function mutations in SHP2 gene. The increase in excitatory neurons (ENs) and overexpression of cortical layer markers are observed in NS-COs, along with reduced synaptic connectivity. These findings suggest that perturbed cortical layer identity and impeded neuronal connectivity contribute to the neurological manifestations of NS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhenming Guo, Mengxia Chen, Yiming Chao, Chunhai Cai, Liangjie Liu, Li Zhao, Linbo Li, Qing-Ran Bai, Yanxin Xu, Weibo Niu, Lei Shi, Yan Bi, Decheng Ren, Fan Yuan, Shuyue Shi, Qian Zeng, Ke Han, Yi Shi, Shan Bian, Guang He
Summary: RGCC regulates NSC self-renewal and neuronal differentiation by affecting cell cycle regulation and spindle orientation, with deficiency leading to decreased NSC population and brain developmental malformation. This study suggests that RGCC plays a crucial role in maintaining the NSC pool during cortical development and potential etiological roles in human brain malformations.
Review
Cell Biology
Flaminia Kaluthantrige Don, Nereo Kalebic
Summary: The expansion of the cerebral cortex is closely related to the acquisition of higher intellectual abilities that distinguish humans from their closest relatives. Recent studies using brain organoids have provided valuable insights into the molecular and cell biological features of basal radial glia (bRG), which can help understand the onset of neurodevelopmental disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Franz Markert, Alexander Storch
Summary: Oxygen tension affects cortical development, and hyperoxygenation during mid-neurogenesis phase accelerates cortical development and increases the number of cortical layer 5 neurons until birth.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Virology
Emmanuel C. Ijezie, John M. O'Dowd, Man Kuan, Alexandra R. Faeth, Elizabeth A. Fortunato
Summary: HCMV infection is the leading cause of virus-induced neurologic birth defects, but the mechanism is still unclear. This study used HCMV-infected induced pluripotent stem cells (iPSCs) to generate in vitro brain organoids and found that infected organoids had fewer neural rosettes and structural deficits. Further experiments showed that HCMV-induced downregulation of NID1 impaired neural rosette formation and integrity.
JOURNAL OF VIROLOGY
(2023)
Review
Genetics & Heredity
Toshikatsu Matsui, Tadahiro Shinozawa
Summary: Organoids, three-dimensional structures fabricated in vitro, mimic complex microenvironments and physiological functions, bridging the gap between animals and humans in research. In toxicology and drug development, human organoids offer new possibilities by overcoming limitations of current models.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Liang Luo, Wei Zhang, Jing Wang, Ming Zhao, Kuo Shen, Yanhui Jia, Yan Li, Jian Zhang, Weixia Cai, Dan Xiao, Xiaozhi Bai, Kaituo Liu, Kejia Wang, Yue Zhang, Huayu Zhu, Qin Zhou, Dahai Hu
Summary: A new 3D culture method for adipose-derived mesenchymal stem cells based on a self-feeder layer has been developed, showing better proliferation ability and cell viability compared to traditional ultra-low attachment (ULA) methods. The unique properties of the SLF-3D spheroid are likely due to changes in the expression of ECM-related genes, such as COL3A1, MMP3, HAS1, and FN1, making it a promising approach for future clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Neurosciences
Pan Fan, YuanHao Wang, Min Xu, Xiao Han, Yan Liu
Summary: This study reviews the recent advances in brain organoid techniques and their application in assessing neural toxicants. Brain organoids with self-assembled 3D structures provide opportunities for testing and predicting human neurotoxicity.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Engineering, Biomedical
Afonso Malheiro, Abhishek Harichandan, Joyce Bernardi, Adrian Seijas-Gamardo, Gonda F. Konings, Paul G. A. Volders, Andrea Romano, Carlos Mota, Paul Wieringa, Lorenzo Moroni
Summary: In this study, a functional humanized in vitro nerve model using induced pluripotent stem cells-derived nociceptors was developed. The model demonstrated realistic responses to stimuli and had enhanced tissue development and stability using a scaffold. Additionally, the model showed potential for integrating other organ cells for more comprehensive studies.
Article
Environmental Sciences
Yan Huang, Yanping Dai, Min Li, Lulu Guo, Chulin Cao, Yuting Huang, Rui Ma, Shengyue Qiu, Xiaoyi Su, Kai Zhong, Yina Huang, Hong Gao, Qian Bu
Summary: Exposure to cadmium resulted in apoptosis of neurons, inhibition of neural progenitor cell proliferation, overexpression of GFAP, release of IL-6, disturbed mineral absorption, and impaired ciliogenesis.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Nanoscience & Nanotechnology
Eunseon Jeong, Suah Choi, Seung-Woo Cho
Summary: Brain organoids are a new model system for studying neurodevelopment and disorders, but they are not as mature and functional as real human brains. To overcome these limitations, various engineering techniques have been employed to improve their development, reduce heterogeneity, and facilitate functional analysis and monitoring.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Veterinary Sciences
Laurence Finot, Eric Chanat, Frederic Dessauge
Summary: Advances in stem cell identification and culture have enabled the derivation of in vitro 3D tissue structures that mimic the in vivo functioning of organs, such as the mammary gland. This provides a useful tool for studying mammary gland development, milk secretion processes, and the effects of infections at the cellular level, with the aim of improving animal milk production.
VETERINARY RESEARCH
(2021)
Article
Cell Biology
Keiko Muguruma, Ayaka Nishiyama, Hideshi Kawakami, Kouichi Hashimoto, Yoshiki Sasai
Article
Neurosciences
Hiroyuki Morino, Yukiko Matsuda, Keiko Muguruma, Ryosuke Miyamoto, Ryosuke Ohsawa, Toshiyuki Ohtake, Reiko Otobe, Masahiko Watanabe, Hirofumi Maruyama, Kouichi Hashimoto, Hideshi Kawakami
Article
Neurosciences
Noriomi Eguchi, Akitoyo Hishimoto, Ichiro Sora, Masahiro Mori
NEUROSCIENCE LETTERS
(2016)
Article
Cell Biology
Yoshihito Ishida, Hideshi Kawakami, Hiroyuki Kitajima, Ayaka Nishiyama, Yoshiki Sasai, Haruhisa Inoue, Keiko Muguruma
Article
Cell & Tissue Engineering
Tokushige Nakano, Satoshi Ando, Nozomu Takata, Masako Kawada, Keiko Muguruma, Kiyotoshi Sekiguchi, Koichi Saito, Shigenobu Yonemura, Mototsugu Eiraku, Yoshiki Sasai
Review
Cell Biology
Keiko Muguruma, Yoshiki Sasai
DEVELOPMENT GROWTH & DIFFERENTIATION
(2012)
Article
Medicine, Research & Experimental
Ryusaku Matsumoto, Hidetaka Suga, Takashi Aoi, Hironori Bando, Hidenori Fukuoka, Genzo Iguchi, Satoshi Narumi, Tomonobu Hasegawa, Keiko Muguruma, Wataru Ogawa, Yutaka Takahashi
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Review
Neurosciences
Atsushi Tamada, Shoji Watanabe, Keiko Muguruma
MOLECULAR AND CELLULAR NEUROSCIENCE
(2020)
Article
Multidisciplinary Sciences
Mariko Taniguchi-Ikeda, Michiyo Koyanagi-Aoi, Tatsuo Maruyama, Toru Takaori, Akiko Hosoya, Hiroyuki Tezuka, Shotaro Nagase, Takuma Ishihara, Taisuke Kadoshima, Keiko Muguruma, Keiko Ishigaki, Hidetoshi Sakurai, Akira Mizoguchi, Bennett G. Novitch, Tatsushi Toda, Momoko Watanabe, Takashi Aoi
Summary: This study successfully modeled the phenotypes of Fukuyama congenital muscular dystrophy (FCMD) patients by using induced pluripotent stem cells (iPSCs) and differentiated brain organoids and skeletal muscle. It found the importance of alpha DG O-mannosyl glycans for normal RG fiber architecture and proper neuronal migration in corticogenesis. Additionally, the compound Mannan-007 (Mn007) showed promising results in restoring alpha DG glycosylation and partially rescuing abnormal RG fiber migration.
Article
Multidisciplinary Sciences
Hajime Ozaki, Hidetaka Suga, Mayu Sakakibara, Mika Soen, Natsuki Miyake, Tsutomu Miwata, Shiori Taga, Takashi Nagai, Mayuko Kano, Kazuki Mitsumoto, Takashi Miyata, Tomoko Kobayashi, Mariko Sugiyama, Takeshi Onoue, Hiroshi Takagi, Daisuke Hagiwara, Shintaro Iwama, Ryoichi Banno, Genzo Iguchi, Yutaka Takahashi, Keiko Muguruma, Haruhisa Inoue, Hiroshi Arima
Summary: In this study, a human in vitro model of familial neurohypophyseal diabetes insipidus (FNDI) was developed by converting human induced pluripotent stem cells (iPSCs) using a naive conversion kit. The converted iPSCs showed improved cell survival and differentiation into AVP neurons, allowing for the study of FNDI pathology.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Danmeng Zhang, Noriomi Eguchi, Satoshi Okazaki, Ichiro Sora, Akitoyo Hishimoto
Summary: Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder that affects individuals from childhood to adulthood. In this study, induced pluripotent stem cell (iPSC)-derived telencephalon organoids were used to recreate the alterations observed in the early cerebral cortex of ADHD patients. The ADHD-derived organoids exhibited slower growth of layer structures, increased neuron density, decreased cell proliferation, altered cell division patterns, and increased cell apoptosis during early development. These findings provide insight into the pathological mechanisms of ADHD and support the use of organoid models for studying neurodevelopmental disorders.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Neurosciences
Keiko Muguruma
Article
Developmental Biology
Atsushi Shiraishi, Keiko Muguruma, Yoshiki Sasai
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)