Review
Cell Biology
Diana Pereira, Ines Sequeira
Summary: Epithelial tissues have a natural ability for renewal and regeneration, crucial for providing the ultimate barrier against the external environment. The oral mucosa is known for its outstanding healing potential with minimal scarring, contributing to reducing fibrotic tissue formation. The combination of cell therapy and bioengineering to leverage the scarless properties of the oral mucosa for skin and esophageal wound healing is a promising avenue for achieving scarless wound healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Maaike Waasdorp, Bastiaan P. Krom, Floris J. Bikker, Paul P. M. van Zuijlen, Frank B. Niessen, Susan Gibbs
Summary: Oral mucosa heals faster, shows a more rapid immune response, and undergoes increased extracellular matrix remodeling compared to skin, leading to superior wound healing and reduced scar formation.
Article
Biotechnology & Applied Microbiology
Yanfen Zheng, Zhiguo Li, Chuyi Liu, Xiaotong Fan, Zheng Luo, Zibiao Li, Yun-Long Wu
Summary: This study developed a multi-functional GOx-CAT nanogel (GCN) system for the treatment of diabetic oral mucosa ulcers. The results showed that the nanoscale GCN was capable of significantly eliminating intracellular ROS, increasing intracellular oxygen concentration and accelerating cell migration of human gingival fibroblasts, which could promote diabetic oral gingival ulcer healing in vivo by alleviating inflammation and promoting angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Dermatology
Michelle F. Griffin, Evan J. Fahy, Megan King, Nicholas Guardino, Kellen Chen, Darren B. Abbas, Christopher V. Lavin, Nestor M. Diaz Deleon, H. Peter Lorenz, Michael T. Longaker, Derrick C. Wan
Summary: Research into oral mucosa healing as an example of scarless healing provides insight into potential mechanisms for achieving scarless healing in adult skin, with a focus on immune cells, fibroblasts, keratinocytes, and signaling molecules. Further studies are needed to fully understand how the oral niche and stem cell populations contribute to this process, leading to potential therapeutic strategies for reducing scarring in skin healing.
ADVANCES IN WOUND CARE
(2022)
Article
Dermatology
Jennifer Jahel Velez Gonzalez, Maximilian Berger, Stefan Schiele, Anna Rubeck, Gernot Mueller, Julia Welzel, Sandra Schuh
Summary: This study investigated the use of D-OCT for evaluating blood vessel morphology and density in venous ulcers and found that it can provide new information about vascular changes during the healing process.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Goncalo de Jesus, Lara Marques, Nuno Vale, Rui Amaral Mendes
Summary: This experimental study aims to assess the influence of chitosan on the healing process of oral mucosa and the result shows that chitosan can alleviate inflammation and accelerate the healing process of oral mucosa.
Article
Urology & Nephrology
Connor Policastro, Joshua Sterling, Baylee Porter, Daniel J. Zaccarini, Guanqun Li, Gennady Bratslavsky, Dmitriy Nikolavsky
Summary: This study aimed to investigate the impact of tobacco use on oral mucosal tissue harvested for urethroplasty. The results showed no clear or clinically significant histologic or immunohistochemical differences between patients with and without a history of tobacco use.
Review
Biochemistry & Molecular Biology
Hector F. Pelaez-Prestel, Jose L. Sanchez-Trincado, Esther M. Lafuente, Pedro A. Reche
Summary: The oral mucosa is a highly active site of immune activity where various immune cells play a crucial role in defending against pathogens. It is exposed to a variety of antigens from food and commensal bacteria that need to be tolerated, with mechanisms involving dendritic cells, regulatory T cells, and epithelial cells contributing to this tolerance. These tolerogenic mechanisms in the oral mucosa are intricately intertwined.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Hye Rin Kim, Keunbada Son, Young-Tak Son, Yong-Gun Kim, Kyu-Bok Lee, Seung Cheol Lee, Jo-Young Suh, Jae Mok Lee
Summary: This study examined the healing patterns of dental diode lasers and a steel scalpel in periodontal surgery. The results showed that laser-treated wounds had slower healing rates compared to scalpel-treated wounds, but no significant difference was observed in histological and immunohistochemical analysis between the laser and scalpel groups.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Fenting Lei, Fancai Zeng, Xin Yu, Yiping Deng, Zongquan Zhang, Maochang Xu, Nianhui Ding, Ji Tian, Chunhong Li
Summary: Due to the failure of oral nano-delivery systems to target the colon and achieve controlled drug release in the treatment of inflammatory bowel disease (IBD), there is an urgent need to develop a microenvironment responsive drug delivery system. In this study, a pH-responsive hydrogel-coated nanoemulsion was successfully developed for the co-delivery of curcumin and emodin. The prepared system showed promising therapeutic effects in IBD by alleviating inflammation, repairing mucosal damage, and clearing reactive oxygen species.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Surgery
Billur Sezgin, Sedat Tatar, Sercin Karahuseyinoglu, Gizem Nur Sahin, Yagmur Ergun, Gizem Meric, Kaan Ersoy
Summary: DF result in healing through a thick dermal component, while oral fibroblasts result in faster healing and less scarring through potentially privileged angiogenic and regenerative gene expression.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2021)
Review
Biochemistry & Molecular Biology
Erika B. Danella, Marcell Costa de Medeiros, Nisha J. D'Silva
Summary: The oral mucosa plays a crucial role in protecting against physical, microbial, and chemical damage. When compromised, the wound healing response is activated, which involves processes such as immune infiltration, re-epithelialization, and stroma remodeling, all controlled by cytokines that promote cellular migration, invasion, and proliferation. These same cytokines are also implicated in cancer dissemination. Therefore, studying the cytokines involved in oral wound healing can provide insights into the cytokines exploited by oral squamous cell carcinoma (SCC) to promote tumor development and progression.
Article
Environmental Sciences
Yifeng Qian, Ya Gao, Binxin Cai, Wenbin Zhang, Xudong Wang, Renjie Chen
Summary: The study found that low ambient temperature is associated with increased risks of oral diseases, particularly affecting patients above the age of 60. The impact of low temperature can last up to 7 days.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Pharmacology & Pharmacy
Elena Mazzinelli, Ilaria Favuzzi, Alessandro Arcovito, Raffaella Castagnola, Giorgia Fratocchi, Alvaro Mordente, Giuseppina Nocca
Summary: The route of drug administration involving crossing the oral mucosa is highly favored due to its advantages. This review aims to describe the various models used to study drug permeability through the oral mucosa and highlight the most effective ones. There is a growing need for standardized models to develop new drug delivery systems, and Oral Mucosa Equivalents (OMEs) may provide a promising solution.
Article
Biotechnology & Applied Microbiology
Jie Xu, Zhenghao Zhang, Xiaofeng Ren, Yunan Zhang, Yang Zhou, Xiaorong Lan, Ling Guo
Summary: Oral mucosal wounds are susceptible to inflammation due to exposure to microorganisms. This study investigated the use of ginsenoside Rg1 in a hydrogel scaffold to treat mucosal defects in rats. The results showed that Rg1-hydrogel exhibited excellent properties and significantly improved wound healing, as evidenced by increased re-epithelialization, collagen deposition, and decreased inflammation. Molecular biology studies confirmed that Rg1-hydrogel stimulated repair-related factors and inhibited inflammatory factors secretion. These findings suggest that Rg1-hydrogel holds promise for the therapeutic management of oral mucosal wounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Letter
Immunology
Tomoko Kadowaki, Yu Yamaguchi, Mizuho A. Kido, Takaya Abe, Kohei Ogawa, Mitsuko Tokuhisa, Weiqi Gao, Kuniaki Okamoto, Hiroshi Kiyonari, Takayuki Tsukuba
CELLULAR & MOLECULAR IMMUNOLOGY
(2020)
Article
Neurosciences
Koji Tanaka, Shoji Matsumoto, Takeshi Yamada, Ryo Yamasaki, Makoto Suzuki, Mizuho A. Kido, Jun-Ichi Kira
FRONTIERS IN NEUROSCIENCE
(2020)
Article
Multidisciplinary Sciences
Mitsuko Tokuhisa, Tomoko Kadowaki, Kohei Ogawa, Yu Yamaguchi, Mizuho A. Kido, Weiqi Gao, Masahiro Umeda, Takayuki Tsukuba
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Ryoji Joko, Daisuke Yamada, Masahiro Nakamura, Aki Yoshida, Shota Takihira, Tomoka Takao, Ming Lu, Kohei Sato, Tatsuo Ito, Toshiyuki Kunisada, Eiji Nakata, Toshifumi Ozaki, Takeshi Takarada
Summary: PRRX1 has been identified as a marker of limb bud mesenchymal cells, and its expression in human osteosarcoma is correlated with poor prognosis and lung metastasis. Downregulation of PRRX1 can suppress tumor proliferation and invasion, increase sensitivity to chemotherapy drugs, and reduce tumor size and metastasis rates in mouse models. The chemical compound forskolin, identified through RNA expression signatures during PRRX1 knockdown, shows similar effects on tumor proliferation and cell migration as PRRX1 knockdown.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Rei Nishimoto, Sandra Derouiche, Kei Eto, Aykut Deveci, Makiko Kashio, Yoshitaka Kimori, Yoshikazu Matsuoka, Hiroshi Morimatsu, Junichi Nabekura, Makoto Tominaga
Summary: Microglia maintain central nervous system homeostasis by monitoring changes in their environment and taking protective actions, with their movement being modulated by temperature. This temperature-dependent movement may have potential clinical applications in preserving cell function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Akihiro Miura, Daisuke Yamada, Masahiro Nakamura, Shuta Tomida, Dai Shimizu, Yan Jiang, Tomoka Takao, Hiromasa Yamamoto, Ken Suzawa, Kazuhiko Shien, Masaomi Yamane, Masakiyo Sakaguchi, Shinichi Toyooka, Takeshi Takarada
Summary: Utilizing human induced pluripotent stem cells (hiPSCs), researchers established iHER2-hiPSCs and differentiated lung progenitors to form human lung organoids (HLOs), demonstrating that overexpression of HER2 leads to increased morphological irregularity and proliferation capacity in lung tissue, resembling precancerous lesions. HiPSC-derived HLOs serve as a model to study the early tumorigenesis of lung adenocarcinoma and provide insights into the molecular basis of tumor initiation and progression.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Engineering, Biomedical
Daisuke Yamada, Masahiro Nakamura, Tomoka Takao, Shota Takihira, Aki Yoshida, Shunsuke Kawai, Akihiro Miura, Lu Ming, Hiroyuki Yoshitomi, Mai Gozu, Kumi Okamoto, Hironori Hojo, Naoyuki Kusaka, Ryosuke Iwai, Eiji Nakata, Toshifumi Ozaki, Junya Toguchida, Takeshi Takarada
Summary: A protocol has been developed for the selective differentiation of human pluripotent stem cells into expandable PRRX1(+) limb-bud-like mesenchymal cells, allowing for the prospective assessment of their chondrogenic potential and the formation of hyaline cartilaginous-like particles. This method may facilitate the mass production of chondrocytes and cartilaginous tissues for applications in drug screening and tissue engineering.
NATURE BIOMEDICAL ENGINEERING
(2021)
Article
Dentistry, Oral Surgery & Medicine
Atsushi Danjo, Reona Aijima, Reiko U. Yoshimoto, Shin-Ichi Tanaka, Shuhei Iwamoto, Takeshi Katsuki, Shigehisa Aoki, Mizuho A. Kido, Yoshio Yamashita
Summary: This study compared bone healing after osteotomy with ultrasonic osteotomy devices (UODs) and rotary osteotomy devices (RODs). The results showed that UODs had smoother cuts, faster bone healing, and greater quantity of newly formed bone compared to RODs. Osteoblast-like cells harvested from UOD-cut bone chips had higher proliferative activity. It is presumed that UODs stimulate the differentiation of mesenchymal cells into osteoblasts and cause less damage to the bone, thereby assisting bone regeneration.
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY
(2022)
Article
Cell & Tissue Engineering
Tomoka Takao, Hirotaka Masuda, Takashi Kajitani, Fumie Miki, Kaoru Miyazaki, Yushi Yoshimasa, Satomi Katakura, Shoko Tomisato, Sayaka Uchida, Hiroshi Uchida, Mamoru Tanaka, Tetsuo Maruyama
Summary: This study identified the HHUA cell line as a useful tool for discovering and identifying endometrial CSC-selective drugs. The drug sorafenib showed potential as an effective anti-endometrial cancer treatment targeting endometrial CSCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Neurosciences
Makiko Kashio, Satoru Masubuchi, Makoto Tominaga
Summary: The temperature thresholds of TRPM2 channels can be regulated by cytosolic Ca2+ and TRPM2 phosphorylation, providing structural evidence for the determination and regulation of temperature thresholds in thermo-TRP channels.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kohki Maruo, Megumi Nishiyama, Yuko Honda, Ai-Lin Cao, Wei-Qi Gao, Kentaro Shibata, Yuzo Murata, Mizuho A. Kido
Summary: Patients with diabetes have high salivary glucose levels, and the topological changes of glucose transporters may play a role in this.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Cell Biology
Kenjiro Matsumoto, Fumika Sugimoto, Toshiki Mizuno, Taisei Hayashi, Ririka Okamura, Takuya Nishioka, Hiroyuki Yasuda, Syunji Horie, Mizuho A. Kido, Shinichi Kato
Summary: TRPV2 and TRPV1 expressions are upregulated in the TNBS-induced colitis model, with TRPV2 mainly present in intrinsic primary afferent neurons and inhibitory motor neurons in the colon, while TRPV1 mainly present in sensory neurons. Both TRPV2 and TRPV1 antagonists can alleviate visceral hypersensitivity in the TNBS-induced colitis model.
CELL AND TISSUE RESEARCH
(2023)
Article
Medicine, Research & Experimental
Ailin Cao, Weiqi Gao, Takeshi Sawada, Reiko U. Yoshimoto, Reona Aijima, Yasuyoshi Ohsaki, Mizuho A. Kido
Summary: Allergic inflammation in the bronchi can cause hypersensitive skin in the facial skin, potentially leading to mechanical hypersensitivity. This hypersensitivity may be the result of TRPV1-mediated neuronal plasticity and glial activation in the trigeminal ganglion.
LABORATORY INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Jing Lei, Reiko U. Yoshimoto, Takeshi Matsui, Masayuki Amagai, Mizuho A. Kido, Makoto Tominaga
Summary: In this study, the authors identified a transmembrane protein, TMEM79, that negatively regulates the function of TRPV3, an ion channel involved in thermosensation. They found that TMEM79 can suppress TRPV3-mediated currents and alter thermal preference in mice.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Daisuke Yamada, Tomoka Takao, Masahiro Nakamura, Toki Kitano, Eiji Nakata, Takeshi Takarada
Summary: This study found that high pluripotent cell density suppresses the expression of PRRX1 mRNA and tdTomato during the induction of PRRX1(+) LBM-like cells from hPSCs. Through RNA sequencing and flow cytometry, CD44(high) CD140B(high) CD49f(-) were identified as the surface antigens for PRRX1-tdTomato(+) LBM-like cells. These results were validated in different hPSC lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)