Article
Biotechnology & Applied Microbiology
Wenting Gao, Ying Yin, Pan Wang, Wei Tan, Mingliang He, Jianping Wen
Summary: In this study, the genes of the Dahms xylose-utilization pathway were integrated into Bacillus subtilis and a metabolic control module was designed, resulting in an increased yield of fengycin. Metabonomic analysis revealed the underlying factors for the increase of fengycin titer.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Li-Long Shi, Yuanmin Zheng, Bi-Wei Tan, Zheng-Jun Li
Summary: In this study, the metabolic engineering of Escherichia coli was used to establish an efficient xylose utilization pathway. By blocking the production pathways of acetate, sufficient precursor for downstream biosynthesis reactions was provided.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Huiping Bi, Ge Qu, Shuai Wang, Yibin Zhuang, Zhoutong Sun, Tao Liu, Yanhe Ma
Summary: This study describes a novel method for de novo production of rosavin E in Escherichia coli, achieving a titer of 92.9 mg/L and further optimizing the method to reach 782.0 mg/L. This represents an excellent example of producing a natural product with a disaccharide chain using glycosyltransferase engineering and artificial pathway construction.
METABOLIC ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Pavel A. Domnin, Vladislav A. Parfenov, Alexey S. Kononikhin, Stanislav V. Petrov, Nataliya V. Shevlyagina, Anastasia Yu. Arkhipova, Elizaveta V. Koudan, Elizaveta K. Nezhurina, Alexander G. Brzhozovskiy, Anna E. Bugrova, Anastasia M. Moysenovich, Alexandr A. Levin, Pavel A. Karalkin, Frederico D. A. S. Pereira, Vladimir G. Zhukhovitsky, Elena S. Lobakova, Vladimir A. Mironov, Evgeny N. Nikolaev, Yusef D. Khesuani, Svetlana A. Ermolaeva
Summary: This study investigated the physiological changes in bacteria caused by the combined action of magnetic force and microgravity. The results showed that magnetic force strengthened the effects of microgravity on bacterial metabolism, possibly by clustering bacteria within a small volume and reinforcing the microgravity-driven absence of convectional flows.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Dragan Miscevic, Ju-Yi Mao, Teshager Kefale, Daryoush Abedi, Murray Moo-Young, C. Perry Chou
Summary: This study describes the development of a microbial bioprocess usingEscherichia colito produce high levels of 5-aminolevulinic acid (5-ALA). Through the implementation of the Shemin pathway and CRISPRi interference, metabolic engineering strategies were applied to direct carbon flux and enhance the biosynthesis of 5-ALA, achieving significant production yields.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemical Research Methods
Stefano Camborda, Jan-Niklas Weder, Nadine Toepfer
Summary: CobraMod is a Python package for pathway-centric modification and extension of genome-scale metabolic networks. It integrates data from different metabolic pathway databases and user-curated information, and provides high quality metabolic reconstructions.
Article
Biochemistry & Molecular Biology
Tongxing Zhao, Liru Xu, Lei Zhao, Hongjie Zhang, Yin Li, Yanping Zhang
Summary: The mutations in the two-component system BtsT/BtsS in E. coli can increase glyoxylate uptake and shorten the lag phase. Additionally, higher concentrations of glyoxylate result in shorter lag phases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Qi Li, Ruiying Gao, Yi Zhang, Yufei Zhang, Tieliang Liu, Yu-Cai He, Ming-Ming Zheng
Summary: A novel and efficient route for the synthesis of furfuryl alcohol from corncob was proposed using microwave heating and a silica-supported SO42--TiO2 catalyst, achieving a high furfural yield of 73.3%. Novel recombinant E. coli EF22 cells were successfully constructed for the bioreduction of furfural to furfuryl alcohol, and optimal parameters for the process were identified. The chemobiocatalytic strategy combining the SO42--TiO2/SiO2 chemocatalyst and EF22@SA/Fe3O4 biocatalyst achieved a high furfuryl alcohol productivity of 0.5396 g FFAC per g xylan, highlighting the applicability of chemobiocatalysis.
Article
Chemistry, Multidisciplinary
Qi Li, Ruiying Gao, Yi Zhang, Yufei Zhang, Tieliang Liu, Yu-Cai He, Ming-Ming Zheng
Summary: Chemobiocatalytic synthesis provides an effective approach for the transformation of biomass into high-value products. In this study, a novel and efficient route for the preparation of furfuryl alcohol from corncob was proposed, combining chemical and biological catalysis. The results demonstrated the successful conversion of furfural to furfuryl alcohol through the synergistic effect of catalysts and enzymes.
Article
Biotechnology & Applied Microbiology
Dragan Miscevic, Ju-Yi Mao, Bradley Mozell, Kajan Srirangan, Daryoush Abedi, Murray Moo-Young, C. Perry Chou
Summary: The study applied metabolic engineering and bioprocessing strategies to enhance the heterologous production of PHBV with a modulated 3-HV monomeric fraction in engineered E. coli under different oxygenic conditions. By regulating carbon flux channeling and oxygenic conditions, high-level PHBV biosynthesis with a wide range of 3-HV monomeric fraction was achieved, potentially enabling the fine-tuning of PHBV mechanical properties at the biosynthesis stage. Similar strategies could be applied to enhance bio-based production of chemicals derived from succinyl-CoA.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Smaranika Panda, Vincent Yuen Kin Fung, Jie Fu J. Zhou, Hong Liang, Kang Zhou
Summary: This study successfully engineered E. coli to grow on ethylene glycol as the major carbon source by overexpressing two dehydrogenases, improving cell growth on EG through overexpression of fucO and aldA with a constitutive promoter and supplementing a mixture of amino acids at low concentration. With further development, this strain has the potential to produce valuable chemicals from raw EG derived from plastic and cellulosic waste streams.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Multidisciplinary Sciences
Dorota M. Krzyzanowska, Magdalena Jablonska, Zbigniew Kaczynski, Malgorzata Czerwicka-Pach, Katarzyna Macur, Sylwia Jafra
Summary: Using RNAseq, we compared the transcriptomic responses of Pseudomonas donghuensis P482 to root exudates of tomato and maize. We found that tomato exudates specifically induced nitric oxide detoxification, repair of iron-sulfur clusters, respiration through the cyanide-insensitive cytochrome bd, and catabolism of amino and/or fatty acids. Maize exudates specifically induced the activity of the MexE RND-type efflux pump and copper tolerance. The shared response to exudates was influenced by compounds from the plants and their growth environment.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Annette R. Rowe, Farshid Salimijazi, Leah Trutschel, Joshua Sackett, Oluwakemi Adesina, Isao Anzai, Liat H. Kugelmass, Michael H. Baym, Buz Barstow
Summary: Extracellular electron transfer (EET) enables microbial electron uptake for the synthesis of complex organic molecules, but there are significant gaps in understanding the mechanism and genetics of electron uptake.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Bui Hoang Dang Long, Masahiro Nishiyama, Rintaro Sato, Tomonari Tanaka, Hitomi Ohara, Yuji Aso
Summary: In this study, a novel method for producing glyoxylate from glucose using engineered Escherichia coli BW25113 was proposed. By disrupting certain genes and reinforcing the glyoxylate shunt pathway, a glyoxylate titer of 0.93 ± 0.17 g/L was achieved in flask cultivation. Further overexpression of certain genes resulted in an improvement in glyoxylate production to 1.15 ± 0.02 g/L. Additionally, by expressing a heterologous gene, pyc, the production of glyoxylate was increased to 2.42 ± 0.00 g/L, the highest reported titer and specific productivity of glyoxylate in E. coli to date.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Davinder Lall, Dragan Miscevic, Mark Bruder, Adam Westbrook, Marc Aucoin, Murray Moo-Young, C. Perry Chou
Summary: Strain engineering and bioprocessing strategies were utilized to produce porphobilinogen (PBG) using Escherichia coli as the cell factory. By implementing non-native pathways, directing carbon flux, and utilizing CRISPRi technology for genetic regulation, high PBG titers were achieved with improved stability and accumulation. This study identified potential factors limiting PBG production and demonstrated effective metabolic engineering approaches for enhancing biobased PBG production.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Chemistry, Multidisciplinary
Hiluf T. Fissaha, Grace M. Nisola, Francis Kirby Burnea, Jin Yong Lee, Sangho Koo, Soong-Peong Lee, Kim Hern, Wook-Jin Chung
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2020)
Article
Engineering, Environmental
Grace M. Nisola, Khino J. Parohinog, Min Kyung Cho, Francis Kirby B. Burnea, Jin Yong Lee, Jeong Gil Seo, Seong-Poong Lee, Wook-Jin Chung
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Biotechnology & Applied Microbiology
Angelo B. Banares, Kris Nino G. Valdehuesa, Kristine Rose M. Ramos, Grace M. Nisola, Won-Keun Lee, Wook-Jin Chung
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Correction
Biotechnology & Applied Microbiology
Angelo B. Banares, Kris Nino G. Valdehuesa, Kristine Rose M. Ramos, Grace M. Nisola, Won-Keun Lee, Wook-Jin Chung
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Kristine Rose M. Ramos, Kris Nino G. Valdehuesa, Angelo B. Banares, Grace M. Nisola, Won-Keun Lee, Wook-Jin Chung
BIOTECHNOLOGY LETTERS
(2020)
Review
Green & Sustainable Science & Technology
Teklebrahan G. Weldemhret, Angelo B. Banares, Kristine Rose M. Ramos, Won-Keun Lee, Grace M. Nisola, Kris Nino G. Valdehuesa, Wook-Jin Chung
Article
Biotechnology & Applied Microbiology
Rhudith B. Cabulong, Angelo B. Banares, Grace M. Nisola, Won-Keun Lee, Wook-Jin Chung
Summary: A modified microbial strain capable of producing glycolic acid from d-xylose was engineered to utilize cellobiose as a co-substrate, resulting in improved glycolic acid production with high yield.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Angelo B. Banares, Grace M. Nisola, Kris N. G. Valdehuesa, Won-Keun Lee, Wook-Jin Chung
Summary: The lignocellulosic sugar d-xylose is gaining attention as a low-cost alternative substrate for genetically engineered organisms. Escherichia coli is widely used as the host organism for engineered microbial cell factories, with a deep understanding of its systems. Limited literature reviews focus on using engineered E. coli for converting d-xylose to target compounds, making this review essential for researchers in this field.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Angelo B. Banares, Grace M. Nisola, Kris Nino G. Valdehuesa, Won-Keun Lee, Wook-Jin Chung
Summary: This review discusses the causes of D-xylonic acid accumulation in metabolically engineered XOP-utilizing microorganisms, and how to address this issue through different engineering and synthetic biology techniques, with a focus on bacterial strains. Additionally, the ability of certain microorganisms to produce and tolerate D-xylonic acid is also examined as an important aspect in developing efficient microbial cell factories.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)