Article
Multidisciplinary Sciences
Guanghan Meng, Jian Zhong, Qinrong Zhang, Justin S. J. Wong, Jianglai Wu, Kevin K. Tsia, Na Ji
Summary: Understanding blood flow dynamics is crucial for studying the function of blood vessels. Current methods lack the resolution to monitor blood flow at a cellular level in large vessels. However, using a fast line-scanning module, researchers were able to achieve high-resolution imaging of blood flow in the cortex of awake mice. This breakthrough allowed them to measure cerebral blood flow and observe pulsatile blood flow patterns.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Neurosciences
Kunpeng Chen, Zhaoshi Tian, Lingjie Kong
Summary: This review discusses the importance of optical miniscopes in studying neural activity in freely moving animals, including their fundamental principles, system structures, technical advances, limitations, and future trends.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Shen Tong, Jincheng Zhong, Xinlin Chen, Xiangquan Deng, Jie Huang, Yingxian Zhang, Mengyuan Qin, Zhenhui Li, Hui Cheng, Wanjian Zhang, Lei Zheng, Weixin Xie, Ping Qiu, Ke Wang
Summary: Multiphoton microscopy (MPM) is a powerful technique for visualizing deep-brain structures at high resolution. This study demonstrates the measurement technology for the multiphoton excitation and emission properties of fluorescent labels at the 2200 nm window, and successfully applies it to deep-brain vasculature imaging in vivo.
Article
Chemistry, Multidisciplinary
Shaowei Wang, Xueqi Li, Suet Yen Chong, Xiaoyuan Wang, Huan Chen, Chengjian Chen, Lai Guan Ng, Jiong-Wei Wang, Bin Liu
Summary: Organic dots with aggregation-induced emission (AIEgen) have been developed for in vivo labeling and imaging of lipid-rich tissues, showing high targeting specificity and stability, as well as strong three-photon fluorescence imaging in deep tissues under NIR-II laser excitation. This study will inspire the development of lipid-targeting fluorophores for in vivo applications.
ADVANCED MATERIALS
(2021)
Review
Immunology
Yoshinobu Konishi, Kenta Terai
Summary: Inflammation plays a role in cancer development and progression, and the complex intercellular cross-talks in the tumor microenvironment have not been successfully replicated in vitro. Two-photon excitation microscopes (2P-IVM) with fluorescent indicators are currently used to observe intercellular cross-talks, revealing the cellular and molecular mechanisms in a spatiotemporal context. This review summarizes the current state of 2P-IVM and discusses the potential application of red-shifted indicators and optogenetic tools. In the era of single-cell transcriptomics and data-driven research, 2P-IVM remains crucial in providing the missing spatiotemporal context in cancer research.
INFLAMMATION AND REGENERATION
(2023)
Review
Biochemistry & Molecular Biology
Yi Chen
Summary: This review highlights the use of A beta-specific fluorescent probes for two-photon imaging of A beta plaques in vivo. The synthesis and detection mechanism of these probes, as well as their application in imaging A beta plaques in vivo, are discussed.
Article
Nanoscience & Nanotechnology
Lukasz Bijoch, Urszula Wlodkowska, Rafal Kasztelanic, Monika Pawlowska, Dariusz Pysz, Leszek Kaczmarek, Radek Lapkiewicz, Ryszard Buczynski, Rafal Czajkowski
Summary: In this study, we provide experimental verification for the use of short-section imaging bundles in two-photon microscopy imaging of the mouse brain. The bundle, made of heavy-metal oxide glasses, has a high numerical aperture and allows for successful imaging with high resolution. The system can be used for minimal-invasive in vivo imaging of various brain areas and is cost-effective and easy to operate.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Ophthalmology
Grazyna Palczewska, Maciej Wojtkowski, Krzysztof Palczewski
Summary: The eye is a suitable organ for multi-photon excitation imaging due to the transparency of ocular tissues to infrared light. The interface between the retina and the retinal pigment epithelium (RPE) provides valuable information about the visual cycle and its response to various factors. Vitamin A-derived retinoids and bisretinoids can be detected using two-photon ophthalmoscopy, enabling functional studies of the retina. Recent advancements in safety and data interpretation have allowed for the first studies with humans.
PROGRESS IN RETINAL AND EYE RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Xiangquan Deng, Zhourui Xu, Zhijun Zhang, Wanjian Zhang, Jiangao Li, Lei Zheng, Xinlin Chen, Yi Pan, Ping Qiu, Dong Wang, Gaixia Xu, Ke Wang
Summary: This article introduces a novel fluorescent gene MTTCM for brain imaging. The MTTCM nanoparticles have good biocompatibility and photostability, and exhibit spectral advantages in deep-brain imaging. The study found that MTTCM NPs can be used for imaging under cortical resection up to 1905 μm and intact skull up to 1100 μm, and can be used to record hemodynamic imaging depth.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Analytical
Jia Zhou, Songjie Fang, Duoteng Zhang, Yunwei Qu, Lan Wang, Sijun Pan, Lin Li, Jie Li, Wei Du, Qiong Wu
Summary: This study developed a NTR probe (ZJ) that can accurately and rapidly detect the overexpressed NTR activity in tumor cells. ZJ showed responsive turn-on two-photon excited fluorescence by NTR recognition in both cells and in vivo. The probe demonstrated favorable stability and specificity, and successfully visualized the distribution of overexpressed NTR in hypoxic cancer cells and zebrafish, indicating its potential application in studying the relationship between tumor hypoxic microenvironment and cancer.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Lingling Xu, Nanhui Liu, Wenjun Zhan, Yu Deng, Zhaoxia Chen, Xiaoyang Liu, Ge Gao, Qian Chen, Zhuang Liu, Gaolin Liang
Summary: The study developed a near-infrared fluorescence probe Cy5.5-CBT-NPs for noninvasive and sensitive evaluation of cancer immunotherapy efficacy. The probe enabled real-time monitoring and enhancement of cytotoxic T lymphocytes (CTLs) responses against tumor cells.
Article
Optics
Ke Wang, Wanjian Zhang, Xiangquan Deng, Shen Tong, Hui Cheng, Mengyuan Qin, Lei Zheng, Kun Zhao, Ruizhan Zhai, Zhongqing Jia, Ping Qiu
Summary: Multiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is determined by the emission wavelength of fluorescent labels. This study compared near-infrared (NIR) and orange-red emission wavelengths for 3-photon imaging of mouse brains in vivo. The results showed that NIR emission decays less than orange-red fluorescent emission as the imaging depth increases, indicating that NIR is more efficient for signal collection deep in the brain.
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Wanjun Gong, Lingdong Jiang, Yanxia Zhu, Mengna Jiang, Danyang Chen, Zhaokui Jin, Shucun Qin, Zhiqiang Yu, Qianjun He
Summary: A catalytic hydrogenation strategy was proposed to design and synthesize a ratiometric fluorescent probe, enabling in vitro and in vivo fluorescence imaging of hydrogen molecules. The developed probe exhibits high sensitivity, rapid responsivity, high selectivity, and low detection limit, allowing for rapid and real-time detection of hydrogen molecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Materials Science, Multidisciplinary
Meicen Liu, Guanyu Chen, Lilan Xu, Tingting Chen, Wenqian Chen, Jinghua Chen, Xi Zhang
Summary: Understanding the functions of enzymes is important, but designing probes for fast analysis of enzymatic activity is challenging. A fluorescence-enhanced probe, 10-methyl-2-nitro-acridone (MNA), was synthesized and applied to analyze nitroreductase (NTR) activity. The probe shows low cytotoxicity, fast response, high selectivity and sensitivity, and can be used for fluorescence imaging in other biosystems.
FRONTIERS IN MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Huifang Su, Tao Xie, Yong U. Liu, Yuhan Cui, Wei Wen, Ben Zhong Tang, Wei Qin
Summary: The application of fluorescent probes for in vivo retinal imaging is crucial for understanding eye diseases. A group of organic luminogens with electron-donating and electron-accepting structures were synthesized and found to be efficient emitters. Their resulting fluorescent nanoparticles showed excellent performance as lipid droplets targeting probes. Utilizing these nanoparticles, accurate and detailed imaging of live zebrafish retina was achieved for the first time using two-photon fluorescence microscopic technique.
CHINESE CHEMICAL LETTERS
(2023)
