Article
Materials Science, Multidisciplinary
Ayoub Glia, Muhammedin Deliorman, Pavithra Sukumar, Farhad K. Janahi, Bisan Samara, Ayoola T. Brimmo, Mohammad A. Qasaimeh
Summary: In this work, a novel herringbone microfluidic probe (HB-MFP) was developed for efficient capture of circulating tumor cells (CTCs) expressing specific antigens. The HB-MFP enables high throughput sample processing and offers potential for cancer diagnosis and treatment through multiplexed profiling of CTCs.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Biophysics
Aynur Abdulla, Zhianan Zhang, Khan Zara Ahmad, Antony R. Warden, Hengyu Li, Xianting Ding
Summary: This study demonstrates a novel antibody functionalized microfluidic chip that can rapidly and accurately capture circulating tumor cells (CTCs) from cancer patients' whole blood. The chip offers a valuable tool for cancer screening, prognosis, and diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biology
Jose Montoya Mira, Ajay A. Sapre, Brett S. Walker, Jesus Bueno Alvarez, Kyle T. Gustafson, Eugene Tu, Jared M. Fischer, Melissa H. Wong, Sadik Esener, Yu-Jui Chiu
Summary: Researchers have developed a label-free dielectrophoretic microfluidic platform for the enrichment of circulating hybrid cells (CHCs), showing promising potential for isolating tumor cells non-invasively from patient blood samples within one hour. They have optimized the device using in vitro models and successfully applied it to clinically relevant mutation analyses in 75% of the samples tested.
COMMUNICATIONS BIOLOGY
(2021)
Article
Engineering, Chemical
Xiangzhi Zhang, Xiawei Xu, Yong Ren, Yuying Yan, Aiguo Wu
Summary: An Y-Y shaped microfluidic device was designed to separate NSCLC cells using a dielectrophoresis approach, with a separation efficiency of around 99% achieved under optimal operating conditions. This study presents a potentially efficient, facile, and low-cost route for circulating tumor cell separation, which was optimized through numerical simulations.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Mengyuan Wang, Yulong Tan, Du Li, Gangwei Xu, Di Yin, Yunchao Xiao, Tiegang Xu, Xiaofeng Chen, Xiaoyue Zhu, Xiangyang Shi
Summary: The study developed a negative sorting method for the isolation of circulating tumor cells (CTCs) through a microfluidic platform integrated with streptavidin-functionalized electrospun polylactic-co-glycolic acid nanofibers. This method showed high efficiency in capturing white blood cells (WBCs) and enriching cancer cells, making it promising for cancer diagnosis.
ADVANCED FIBER MATERIALS
(2021)
Article
Engineering, Biomedical
Liang Dong, Xinxing Du, Changxue Lu, Zhongyuan Zhang, Chung-Ying Huang, Lei Yang, Sarah Warren, Morgan D. Kuczler, Diane K. Reyes, Jun Luo, Sarah R. Amend, Wei Xue, Kenneth J. Pienta
Summary: The application of circulating tumor cells (CTCs) is limited by the rare number of targets in blood samples. However, a new protocol combining diagnostic leukapheresis (DLA) and RNA analysis shows promise for capturing and analyzing CTCs from large blood samples. The method demonstrates reliable capture and provides valuable information for disease monitoring.
MATERIALS TODAY BIO
(2022)
Article
Chemistry, Physical
V. Varmazyari, H. Ghafoorifard, H. Habibiyan, M. Ebrahimi, S. Ghafouri-Fard
Summary: This paper presents a hybrid microfluidic platform for label-free cell separation, combining cascade deterministic lateral displacement (DLD) array and traveling wave dielectrophoresis (twDEP) system. It demonstrates efficient separation of CTC clusters and red blood cells, as well as classification and separation of CTCs and white blood cells based on their sizes. The proposed structure outperforms conventional designs in terms of pressure distribution and cell viability.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Analytical
Yisha Huang, Xilin Li, Jianwen Hou, Zhouying Luo, Guang Yang, Shaobing Zhou
Summary: In this study, a simple conductive nanofiber chip (CNF-Chip) embedded microfluidic device with a herringbone microchannel was designed to achieve efficient capture and electrical stimulation-triggered rapid release of circulating tumor cells (CTCs). By utilizing the local topographic interaction between target cells and the nanofibrous substrate, along with the high-throughput microfluidic mixing, the capture efficiency for CTCs was significantly improved (over 85%). Additionally, the sensitive and rapid release of CTCs (release efficiency above 97%) could be achieved through the cleavage of the gold-sulfur bond by applying a low voltage (-1.2 V). This CNF-Chip embedded microfluidic device showed great potential in clinical applications, as it was successfully used for the effective isolation of CTCs in clinical blood samples from cancer patients.
Article
Chemistry, Multidisciplinary
Yan Liu, Zhun Lin, Ziwei Zheng, Yuanqing Zhang, Lingling Shui
Summary: This article presents a multimarker microfluidic chip for the enrichment of heterogeneous circulating tumor cells (CTCs) in breast cancer patients. The chip utilizes a combination of size selection effect and multivalent aptamers to enhance capture efficiency. In addition, the DNA nanostructure-functionalized chip allows for the capture of different phenotypes of CTCs while maintaining molecular integrity and cell viability.
Article
Engineering, Electrical & Electronic
Mate Kalovics, Kristof Ivan, Zsolt Szabo
Summary: A microfluidic mixer is integrated with a dual-band microwave resonator for in situ monitoring of liquid mixing. The mixer utilizes a complementary double split-ring resonator etched in the ground plane of a microstrip transmission line. Through simulations, optimal channel configurations are designed to increase sensitivity, minimize required sample volume, and eliminate unwanted microfluidic mixing. The device allows for unambiguous chemical identification by monitoring the frequency variation of resonances.
IEEE SENSORS JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Tito Palmela Leitao, Patricia Corredeira, Sandra Kucharczak, Margarida Rodrigues, Paulina Piairo, Carolina Rodrigues, Patricia Alves, Ana Martins Cavaco, Miguel Miranda, Marilia Antunes, Joao Ferreira, Jose Palma Reis, Tome Lopes, Lorena Dieguez, Luis Costa
Summary: Renal cell carcinoma (RCC) often presents as metastatic disease. Research on circulating tumor cells (CTCs) and liquid biopsies is improving understanding of RCC biology and metastasis formation. This study aimed to clinically validate the effectiveness of RUBYchip (TM), a microfluidic label-free CTC detection platform, in RCC patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Hyungseok Cho, Jae Il Chung, Jinho Kim, Won Ik Seo, Chan Ho Lee, Todd M. Morgan, Seok-Soo Byun, Jae-Seung Chung, Ki-Ho Han
Summary: The study aimed to isolate circulating tumor cells using a novel microseparator technique, evaluate prostate cancer-specific gene expressions, and develop a multigene model for identifying advanced prostate cancer. Results showed a significant increase in CTC number and purity at different cancer stages, with the multigene model demonstrating higher accuracy in identifying advanced prostate cancer.
Article
Nanoscience & Nanotechnology
Yilong Liu, Rui Su, Juan Song, Xiyuan Yu, Shichao Lin, Zhi Zhu, Yuanyuan Yang, Mingxia Zhang, Liu Yang, Huimin Zhang, Xiuqin Xu, Chaoyong Yang
Summary: The development of a microfluidic chip for noninvasive assessment of myeloma, with high capture efficiency and throughput, enables nondestructive release of target cells for downstream analysis, offering new opportunities for myeloma evaluation and research.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Levi Groen, Iris Kloots, David Englert, Kelly Seto, Lana Estafanos, Paul Smith, Gerald W. Verhaegh, Niven Mehra, Jack A. Schalken
Summary: The clinical utility of circulating tumor cells (CTC) as a non-invasive multipurpose biomarker is well recognized. The earliest methods for enriching CTCs relied on antibody-based positive selection, but it has limitations in capturing the heterogeneity of cancer. This study used the recently FDA-approved Parsortix (R) technology to enrich CTCs from prostate cancer patients and found that targeted CTC transcriptome profiling may be predictive of therapy response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Mahmoud Labib, Shana O. Kelley
Summary: Analysis of circulating tumor cells provides valuable insights in precision oncology, revealing associations between CTCs molecular profiles and tumor response to therapy. However, current limitations hinder the widespread clinical implementation of CTCs, suggesting the need for further research and advanced technologies.
MOLECULAR ONCOLOGY
(2021)