Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Chemistry, Analytical
Rihui Su, Ling Xia, Gongke Li, Xiaohua Xiao
Summary: Temperature-responsive PNIPAM-VPBA microgels with enhanced affinity capability were synthesized for capturing glycoproteins, resulting in signal amplification in colorimetric analysis. The microgels showed potential applications in food and clinical analysis with satisfactory recoveries and low toxicity.
Article
Nanoscience & Nanotechnology
Lin Long, Mingji Zong, Yunqing Xie, Qing Chai, Jianbo Liu, Xiaochun Wu
Summary: A nanoreactor with a hollow Pt nanocage-encapsulated mesoporous SiO2 shell was fabricated using a facile and general strategy. This structure exhibited highly dispersed catalytic Pt nanoparticles inside the cavity enclosed by the SiO2 shell, providing a framework for efficient catalysis. The nanoreactors demonstrated high sensitivity and detection performance when used as biosensors for specific antibodies.
ACS APPLIED NANO MATERIALS
(2022)
Article
Biophysics
Mehdi Dadmehr, Monireh Mortezaei, Behnaz Korouzhdehi
Summary: In this study, a novel and sensitive method for the detection of MMP-9 enzyme was developed using a nanocomposite structure. The constructed nanocomposite exhibited a fluorescence enhancement and changes in surface plasmon resonance, enabling the semi-quantitative detection of MMP-9 enzyme. This approach provides an efficient, convenient, and practical tool for the simple identification and precise detection of MMP-9 enzyme.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
A-Yeung Jang, Min-Joo Choi, Yong Zhi, Hyun-Jung Ji, Ji-Yun Noh, Jin-Gu Yoon, Hee-Jin Cheong, Woo-Joo Kim, Ho-Seong Seo, Joon-Young Song
Summary: The standardized GBS-ELISA developed in this study shows high specificity and accuracy in evaluating the immunogenicity of GBS vaccines against the three main serotypes. The results demonstrate good performance of the ELISA in assessing the three serotypes, aiding in the development and evaluation of GBS vaccines.
Article
Medicine, General & Internal
Lotfi S. Bin Dahman, Khalid M. Sumaily, Essa M. Sabi, Mohammed A. Hassan, Abeer M. Bin Thalab, Asrar S. Sayad, Saleh M. Bin Kolaib, Fatima M. Alhadhrmi
Summary: This study compared the serum ferritin levels measured by ELISA, Cobas e411, and Cobas Integra 400 methods. The results showed no significant difference between ELISA and Cobas e411, while the values from Cobas Integra 400 were significantly higher. Cobas e411 and Cobas Integra 400 methods demonstrated higher sensitivity, specificity, and accuracy. Further investigations with larger samples are needed to determine interchangeability.
Article
Engineering, Environmental
Yidan Ma, Yujie Yu, Xinyi Mu, Chao Yu, Yuan Zhou, Jun Chen, Shuting Zheng, Junlin He
Summary: In this study, a novel dual-analytical platform was developed for visual, sensitive, and reliable analysis of mercury ions in environmental water samples. The method utilized thymine-rich DNA probes and luminescent metalpolydopamine frameworks to achieve accurate detection of Hg2+, with potential applicability for monitoring environmental water samples. Additionally, a multicolorimetric system based on a smartphone was established for on-site determination.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemical Research Methods
Tetsuya Saita, Hiroto Kataoka, Rintaro Sogawa, Tadashi Hayama, Ryoko Tomita, Akira Monji, Yoshito Mizoguchi, Chisato Shimanoe
Summary: The study successfully developed a simple and specific ELISA method for the quantification of PEA, showing comparable results to HPLC measurements and a strong correlation between the values determined by the two methods.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Chemistry, Analytical
Juanjuan Huang, Yiyun Yao, Yanling Chen, Tianran Lin, Li Hou, Dianping Tang
Summary: A CP/ZIF-8/PDA-based FLISA method was developed for sensitive and high-throughput determination of CEA using self-supplied H2O2 generation. The strategy showed great potential for the detection of other biomarkers in biomedicine field.
Article
Chemistry, Physical
Shichao Ding, Zhaoyuan Lyu, Erik Sarnello, Mingjie Xu, Lingzhe Fang, Hangyu Tian, Sam Ellery Karcher, Tao Li, Xiaoqing Pan, John McCloy, Guodong Ding, Qiang Zhang, Qiurong Shi, Dan Du, Jin-Cheng Li, Xiao Zhang, Yuehe Lin
Summary: In this study, a MnOx engineered Fe-N-C single-atom catalyst (Mn-Fe-N-C SAC) was developed to reduce oxidative corrosion and enhance the stability of the oxygen reduction reaction (ORR). The Mn-Fe-N-C SAC exhibited ultralow H2O2 yield and negligible potential shift after continuous potential cycles, demonstrating excellent ORR stability and improved activity compared to common Fe-N-C SACs. The interaction between MnOx and Fe-N-x sites enhanced the intrinsic activity of single iron sites, providing a promising solution for the stability issue of Fe-N-C SACs while maintaining high catalytic activity.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
Article
Chemistry, Physical
Yabin Zhang, Tianyu Li, Luyang Miao, Prabhleen Kaur, Shaojie Men, Qiong Wang, Xin Gong, Yuelin Fang, Congcong Zhai, Shuxiang Zhang, Luqing Zhang, Lei Ye
Summary: The article presents a novel zwitterionic liquid polymer hydrogel with extraordinary mechanical versatility, high transparency, and conductivity, as well as anti-freezing ability, for multifunctional sensor applications. The hydrogel demonstrates excellent conductivity and low-temperature tolerance due to the conductive zwitterionic nanochannels formed by the zwitterionic liquid and methacrylate lysine. Additionally, the hydrogel exhibits high transparency, remarkable stretchability, self-healing properties, adhesiveness to diverse substrates, and high conductivity even at low temperatures, making it a promising candidate for strain sensors and ionic skin in wearable electronic devices.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
Article
Engineering, Environmental
Congcong Zhai, Luyang Miao, Yabin Zhang, Luqing Zhang, He Li, Shuxiang Zhang
Summary: A colorimetric sensor array based on enzyme response analysis was developed for pattern recognition of various metal ions in water quality monitoring. The sensor array successfully identifies and distinguishes 11 species of metal ions using principal component analysis (PCA), and can also distinguish different concentrations of single model analyte and mixtures of contaminants in tap water.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Xin Li, Shichao Ding, Zhaoyuan Lyu, Peter Tieu, Maoyu Wang, Zhenxing Feng, Xiaoqing Pan, Yang Zhou, Xiangheng Niu, Dan Du, Wenlei Zhu, Yuehe Lin
Summary: This work presents the design and synthesis of single-atomic iron doped carbon dots with tunable fluorescence and exceptional oxidase-mimetic activity. Phosphate ions can suppress the activity and restore the photoluminescence of the carbon dots, leading to the development of a dual-mode colorimetric and fluorescence assay for phosphate monitoring.
Article
Multidisciplinary Sciences
Zhaoyuan Lyu, Shichao Ding, Peter Tieu, Lingzhe Fang, Xin Li, Tao Li, Xiaoqing Pan, Mark H. Engelhard, Xiaofan Ruan, Dan Du, Suiqiong Li, Yuehe Lin
Summary: In this study, a single-atomic site catalyst (SASC) with excellent catalytic activity was designed to enhance the portable detection performance of herbicides. The catalyst exhibited superior peroxidase-like (POD-like) activity and was used as a label in lateral flow immunoassay (LFIA) for herbicide detection. The Fe-SASC-LFIA achieved a linear detection range of 1-250 ng/mL with a low limit of detection (LOD) of 0.82 ng/mL for 2,4-dichlorophenoxyacetic acid (2,4-D). The method demonstrated excellent specificity and selectivity towards 2,4-D and showed promising results in the detection of human urine samples, indicating its potential as a portable and high-sensitive point-of-care (POC) detection strategy for pesticide exposure evaluation.
Article
Chemistry, Physical
Shichao Ding, Lin He, Lingzhe Fang, Yuanzhi Zhu, Tao Li, Zhaoyuan Lyu, Dan Du, Yuehe Lin, Jin-Cheng Li
Summary: This study proposes a carbon nanotube-bridging strategy to synthesize a highly efficient oxygen electrocatalyst for the development of high-performance and long-life rechargeable zinc-air batteries. The results show that the developed catalyst exhibits good electrochemical performance and cycle stability.
ADVANCED ENERGY MATERIALS
(2022)
Review
Chemistry, Inorganic & Nuclear
Guohai Yang, Fanglei Liu, Jiayi Zhao, Lijie Fu, Yingqiu Gu, Lulu Qu, Chengzhou Zhu, Jun-Jie Zhu, Yuehe Lin
Summary: In this review, the latest research on MXenes in the fields of biosensing and biomedicine is summarized. The synthesis strategies and unique properties of MXenes are introduced, and the biosensing applications of MXenes are divided into three subcategories based on the sensing mechanism: electrochemical, photochemical, and opto-electrochemical biosensors. The biomedical applications of MXenes are evaluated from four aspects: antibacterial activity, bioimaging, therapeutics, and theranostics. The specific chemical and physical properties of MXenes that influence the efficiency of biosensing and biomedicine are highlighted, as well as the structure-performance relationships determining detection sensitivity and material toxicity. The challenges, future prospects, and opportunities for MXenes are discussed.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Physical
Xiao-Qian Zhang, Zhaoyuan Lyu, Shichao Ding, Shiyong Zhao, Guangyu Chen, Yuehe Lin, Jin-Cheng Li
Summary: A molten-salt-assisted pyrolysis strategy was used to prepare two-dimensional porous metal-nitrogen/carbon materials with exposed metal-nitrogen sites, which showed excellent performance in both liquid-state and solid-state Zn-air fuel cells.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Multidisciplinary
Juan Liu, Xiaoxiao Guo, Zhaoyuan Lyu, Rong-Bin Song, Pengyu Zhou, Shichao Ding, Yang Zhou, Li-Ping Jiang, Yuehe Lin, Wenlei Zhu
Summary: By using copper electrocatalysts and microorganisms, an electro-bio tandem reactor was designed to convert CO2 into specific multicarbon compounds in both the gas and liquid phases. The reducing products from electrocatalysis can be consumed and recycled for selective acetate synthesis in Moorella thermoacetica. The results showed high faradaic selectivity for acetate in liquid products for Cu-based CO2 electroreduction and high electron conversion rate to ethylene and acetate for microbial electrosynthesis, presenting a green design for CO2 upcycling targeting multicarbon products through the parallel integration of electrocatalysis and fermentation.
Review
Chemistry, Multidisciplinary
Weiqing Xu, Yu Wu, Wenling Gu, Dan Du, Yuehe Lin, Chengzhou Zhu
Summary: Metal-organic frameworks (MOFs) have made significant advances as mimics of metalloenzymes in bioinspired catalysis, particularly in the field of active pocket engineering at the atomic level.
CHEMICAL SOCIETY REVIEWS
(2023)
Review
Chemistry, Multidisciplinary
Yifan Chai, Zhaoyuan Lyu, Huitong Du, Pan Li, Shichao Ding, Yujing Jiang, Hua Wang, Qianhao Min, Dan Du, Yuehe Lin, Wenlei Zhu
Summary: This article introduces the fermentative hydrogen production method for biohydrogen and explores the principles of microbial dark fermentation and photo-fermentation in depth. It summarizes the strategies to increase the efficiency of fermentative hydrogen production and describes some recent representative works.
Article
Chemistry, Multidisciplinary
Shuo Feng, Rajesh Kumar Singh, Yucheng Fu, Zhuo Li, Yulong Wang, Jie Bao, Zhijie Xu, Guosheng Li, Cassidy Anderson, Lili Shi, Yuehe Lin, Peter G. Khalifah, Wei Wang, Jun Liu, Jie Xiao, Dongping Lu
Summary: In this study, a simple strategy was demonstrated for constructing low-tortuosity through-pores in both vertical and planar directions of electrodes by casting large particles into single-particle-layer electrodes. Through multi-scale characterizations and simulations, correlations between material/electrode structures, electrolyte permeability, polysulfide migration, and sulfur reactions were elucidated. The high-loading and dense sulfur cathode fabricated by this method delivers a high specific capacity at a very low electrolyte/sulfur ratio.
ENERGY & ENVIRONMENTAL SCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Yu Wu, Weiqing Xu, Lei Jiao, Wenling Gu, Dan Du, Liuyong Hu, Yuehe Lin, Chengzhou Zhu
Summary: Enzymes have great potential in various fields due to their high activity and specificity for catalysis under mild conditions. However, they struggle to adapt to different environments outside of their familiar biological conditions. Nanobiocatalysis, which integrates nanotechnology and enzymatic catalysis, aims to design biocatalysis based on materials science. Nano-integrated biocatalysis and bio-inspired nanocatalysis are two effective nanobiocatalytic systems that can meet different design needs, and their combination opens up even more possibilities. This review summarizes the types, constructions, and functions of nanobiocatalytic systems, analyzing the pros and cons of different strategies. Additionally, it highlights their applications in bioassay, biotherapy, and environmental remediation.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Chemistry, Physical
Hangyu Tian, Wenlei Zhu, Qiurong Shi, Shichao Ding, Zhaoyuan Lyu, Mingjie Xu, Xiaoqing Pan, Mark H. Engelhard, Du Dan, Yuehe Lin
Summary: This work presents a facile method for synthesizing robust Ir-based bimetallic MNNs by chemical reduction. The optimized IrxPb catalyst delivers an enhanced oxygen evolution reaction (OER) performance in acid media, superior to that of the commercial IrO2 catalyst.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
