Article
Chemistry, Analytical
Maria Freitas, Henri P. A. Nouws, Cristina Delerue-Matos
Summary: The development of a 2-step electrochemical immunosensor allows for rapid and accurate detection of peanut allergens in commercial foods, aiding in the prevention of allergic reactions. The sensor achieved a short assay time by combining target analyte and detection antibody beforehand, demonstrating a linear range, precision, and sensitivity suitable for quantifying allergens in various food products.
Article
Agriculture, Multidisciplinary
Yaozhong Hu, Jing Lin, Lijun Peng, Xuemeng Ji, Huan Lv, Jing Wu, Yan Zhang, Shuo Wang, Yi Wang, Sihao Wu
Summary: A nanobody-based micro-total electrochemical immunoassay (Nb-mu TEI) was developed for reliable and accurate detection of peanut allergens, with emphasis on Ara h 1. This study successfully identified the target Ara h 1 using Nb-mediated immunocapturing technique, after immunizing an alpaca and selecting four specific Nbs. The developed Nb-based electrochemical immunoassay exhibited an 11-fold increase in sensitivity compared with the traditional sandwich ELISA, with a linear range of 4.5-55 ng/mL and LOD and LOQ values of 0.86 and 2.10 ng/mL, respectively. The immunoassay was validated by detecting spiked antigens in food samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Xiaoya Zhou, Linmei Ren, Ying Zhang, Jie Zhang, Xin Li, Anshu Yang, Ping Tong, Zhihua Wu, Hongbing Chen
Summary: Protein structure affects the allergenicity, and this study focused on identifying the localized structure that affects the potential allergenicity of Ara h 2. Mutations in amino acids changed the nearby localized structure and the overall structures of the allergen, which affected its IgE binding capacity and reduced its potential allergenicity. The integrity of the disulfide bond and the solvent accessible surface area of aromatic residues were found to be important for the allergenicity of Ara h 2. Additionally, a specific localized structure (R(62)DPYSPSQDPYSPS(75)) was identified as crucial for the allergenicity of Ara h 2.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Danijela Apostolovic, Justin T. Marsh, Joe Baumert, Steve L. Taylor, Adrie Westphal, Harmen de Jongh, Phil Johnson, Govardus A. H. de Jong, Stef J. Koppelman
Summary: Ara h 7, an important peanut allergen from the 2S albumin protein family, has been purified and characterized, showing unique features and presence in all main market types of peanuts.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Applied
Hai Jiang, Qian Guo, Cheng Zhang, Zhikang Sun, Xuan Weng
Summary: This study developed an origami microfluidic electrochemical nano-aptasensor for rapid peanut allergen detection. The sensor could complete detection within 20 min, with a linear range from 50 to 1000 ng/mL and a detection limit of 21.6 ng/mL, successfully detecting Ara h1 spiked cookie dough samples.
Article
Chemistry, Applied
Hsin-Yi Yin, Yi-Ting Li, Wen-Che Tsai, Hong-Yu Dai, Hsiao-Wei Wen
Summary: This study developed an immunomagnetic nanoparticle-based lateral flow assay for detecting the major peanut allergen Ara h 1, with good specificity and sensitivity. The method has the potential to be a rapid tool for screening processed foods for residues of Ara h 1.
Article
Agriculture, Multidisciplinary
Xuejiao Chang, Xiaoya Zhou, Yu Tang, Ying Zhang, Juanli Yuan, Xin Li, Anshu Yang, Ping Tong, Zhihua Wu, Hongbing Chen
Summary: Roasting can alter the structure and allergenicity of peanut protein Ara h 2. After processing, most of the Ara h 2 proteins were fragmented and cross-linked, resulting in structural changes. Roasting enhanced the allergenicity of Ara h 2 monomers, but this does not reflect the overall allergenicity change of Ara h 2 in the matrix.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Fu-Chiun Hsu, Wan-Ting Lin, Kuan-Chen Hsieh, Kuan-Chen Cheng, James Swi-Bea Wu, Yuwen Ting
Summary: Cold argon plasma jet treatment has been shown to reduce the immunoreactivity of peanut protein, providing a potential non-thermal technique to mitigate peanut allergenicity in the food industry.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Chemistry, Analytical
Ching-Chou Wu, Kanishk Singh, Jin-Xin Ye, Yu-Sheng Chuang, Hsiao-Wei Wen
Summary: This study presents a microfluidic biosensing device for detecting peanut allergen using immunoreaction. Under optimal fluidic manipulation, the device can achieve better immunoreaction and has a good linear range and detection limit. The device, integrating electrochemical impedance spectroscopy, exhibits rapid and ultrasensitive sensing properties for detecting allergens in food products.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Allergy
Hans J. M. Warmenhoven, Luuk Hulsbos, Stephen C. Dreskin, Jaap H. Akkerdaas, Serge A. Versteeg, Ronald van Ree
Summary: The study found unexpected cross-reactivity between major peanut allergens Ara h 1, 2, and 3. Cross-contamination of purified natural Ara h 1 and Ara h 3 with Ara h 2 and Ara h 6 was observed, leading to misinterpretation of molecular cross-reactivity. Recombinant Ara h 1 and Ara h 3 are recommended as preferred alternatives for diagnostic testing.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Nutrition & Dietetics
Mengqi Zhang, Liang Liu, Cui Yang, Zhongyu Sun, Xiuhua Xu, Lian Li, Hengchang Zang
Summary: This study used near-infrared spectroscopy combined with aquaphotomics to investigate the structural changes of Ara h1 during the heating process. The results showed that around 55 degrees Celsius was a key temperature point, where the hydrogen bond network was disrupted, free water content increased, and protein secondary structure changed.
FRONTIERS IN NUTRITION
(2021)
Article
Acoustics
Miao Yu, Yuetong Zhou, Xiaohe Wang, Mengxi Xie, Baiqing Zhang, Haiqiu Yu, Zhanxiang Sun
Summary: The study demonstrated that ultrasonication can effectively reduce the Ara h 1 protein content in peanut sprouts, as confirmed by ELISA and protein structure analysis. Ultrasonication also altered proteinase activity and gene expression, significantly decreasing the Ara h 1 gene expression in the ultrasonication group.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sudip Biswas, Nancy J. Wahl, Michael J. Thomson, John M. Cason, Bill F. McCutchen, Endang M. Septiningsih
Summary: In this study, the CRISPR-Cas9 genome editing technology was successfully applied to disrupt a major allergen gene in peanut protoplasts. The polyethylene glycol (PEG)-mediated protoplast transformation system proved to be a rapid and effective tool for transient expression analysis and sgRNA validation in peanut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Maria Freitas, Marta M. P. S. Neves, Henri P. A. Nouws, Cristina Delerue-Matos
Summary: An electrochemical dual immunosensor was developed for efficient detection of peanut allergens in food products. The sensor achieved precise results with low limits of detection and was successfully applied to analyze various food products, confirming its accuracy and practicality for tracking food allergens.
Article
Chemistry, Analytical
Shuo Qi, Anqi Sun, Xiaoze Dong, Yuhan Sun, Minwei Qin, Ning Ding, Yin Zhang, Zhouping Wang
Summary: Food allergy, a serious immune allergy reaction, has gained worldwide attention due to the increasing incidence. Ara h1, the most abundant allergenic protein in peanuts, has been identified as a biomarker for peanut allergen detection. This study developed a simple and efficient fluorescent aptasensor for ultrasensitive and rapid detection of peanut allergen Ara h1.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)