Review
Cell Biology
Xueqi Wang, Yiming Guan, Shiyu Xiang, Karen L. Clark, Peter G. Alexander, Lauren E. Simonian, Yuhao Deng, Hang Lin
Summary: This review examines the potential of mesenchymal stromal cells (MSCs) in cartilage repair based on animal studies conducted in the past three decades. However, the cartilage tissue derived from MSCs does not possess the same characteristics as native hyaline cartilage, especially in terms of chondrocytic hypertrophy. This undesired phenotype needs to be controlled before MSCs can be effectively used for cartilage repair in clinical settings. Current research focuses on suppressing hypertrophy and modulating the Wnt/β-catenin pathway to inhibit hypertrophy, along with exploring the potential crosstalk between Wnt/β-catenin and other pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Dhanalakshmi Jeyachandran, Monzur Murshed, Lisbet Haglund, Marta Cerruti
Summary: Bone tissue engineering often fails due to lack of oxygen, but using a Bioglass-Poly(lactic-co-glycolic acid@fibrin) construct may overcome this issue by promoting the formation of a cartilage template that can survive in hypoxic environments. The construct induces hypertrophy and matrix mineralization, supporting the different phases of endochondral ossification. This approach may decrease cell culture time and complexity associated with external induction factors, facilitating clinical translation.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Jake Littman, Roy K. Aaron
Summary: Notable characteristics of the skeleton include its response to physical stimuli and ability to remodel in different environments. Cells in bones and cartilage can sense physical cues and activate genes to synthesize structural molecules. This review explores the response of a model system of bone formation to an externally applied pulsed electromagnetic field (PEMF), focusing on cell differentiation and extracellular matrix synthesis. The study emphasizes the dosage and tissue response mechanisms, which can guide the design of clinically optimal stimulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Pingping Yuan, Zhiye Li, Bo Shao, Tian Zeng, Xiaopeng Wu, Yinggang Wang, Yimin Zhao, Wei Wu
Summary: In this study, BMSCs pre-conditioned in a starving environment were found to enhance the anti-apoptosis potential of co-transplanted chondrocytes, and extracellular vesicles derived from starving BMSCs played essential roles in improving apoptosis and regulating autophagy of chondrocytes. In vivo studies confirmed that these EVs significantly improved the survival of chondrocyte bricks.
ACTA BIOMATERIALIA
(2022)
Article
Multidisciplinary Sciences
Kosei Nagata, Hironori Hojo, Song Ho Chang, Hiroyuki Okada, Fumiko Yano, Ryota Chijimatsu, Yasunori Omata, Daisuke Mori, Yuma Makii, Manabu Kawata, Taizo Kaneko, Yasuhide Iwanaga, Hideki Nakamoto, Yuji Maenohara, Naohiro Tachibana, Hisatoshi Ishikura, Junya Higuchi, Yuki Taniguchi, Shinsuke Ohba, Ung-il Chung, Sakae Tanaka, Taku Saito
Summary: The transcription factors Runx2 and Runx3 play different roles in the development of osteoarthritis. Runx3 protects articular cartilage by regulating the expression of lubricin and aggrecan, while Runx2 exerts biphasic effects on osteoarthritis development under different conditions.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Yawei Gu, Sebastien Pigeot, Lucas Ahrens, Fabian Tribukait-Riemenschneider, Melika Sarem, Francine Wolf, Andres Garcia-Garcia, Andrea Barbero, Ivan Martin, V. Prasad Shastri
Summary: By developing a bioink system with appropriate rheological properties and using 3D bioprinting technology, it is possible to create high cell density structures of predefined geometry, which can then be implanted into nude mice to generate bone tissue with neovascularization. This method demonstrates the potential for fabricating complex bone tissue for clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Endocrinology & Metabolism
Nicole Bell, Sanket Bhagat, Shanmugam Muruganandan, Ryunhyung Kim, Kailing Ho, Rachel Pierce, Elena Kozhemyakina, Andrew B. Lassar, Laura Gamer, Vicki Rosen, Andreia M. Ionescu
Summary: This study investigates the role of FoxA factors in chondrocyte differentiation and hypertrophy. The researchers found that overexpression of FoxA2 led to skeletal defects and accumulation of hypertrophic chondrocytes in transgenic mice. However, the ectopic expression of hypertrophic markers was only observed in hypertrophic chondrocytes, suggesting the involvement of other factors like Runx2 in inducing chondrocyte hypertrophy.
Article
Anatomy & Morphology
Roshni Parameswaran, Upasana Kachroo, Soosai Manickam Amirtham, Grace Rebekah, Elizabeth Vinod
Summary: The study found that varying osmolarities in culture did not significantly improve chondrogenic potential of chondroprogenitors, but hyperosmotic stress led to an increased tendency for hypertrophy and terminal differentiation.
Article
Cell & Tissue Engineering
K. Futrega, E. Music, P. G. Robey, S. Gronthos, R. Crawford, S. Saifzadeh, T. J. Klein, M. R. Doran
Summary: The study characterized ovine BMSC and evaluated their ability to repair cartilage defects, concluding that micro-pellets manufactured from ovine articular chondrocytes were more effective than those from ovine BMSC. While ovine BMSC could form cartilage-like tissue in vitro, their effective application in cartilage repair will require successful mitigation of hypertrophy and tissue integration issues.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
Hwan D. Kim, Xuechong Hong, Young-Hyeon An, Mihn Jeong Park, Do-Gyoon Kim, Arin K. Greene, Bonnie L. Padwa, Nathaniel S. Hwang, Ruei-Zeng Lin, Juan M. Melero-Martin
Summary: A biomimetic scaffold has been developed to rapidly regenerate bone tissue in animal models, showing promise for application in human bone regeneration.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Nadine Frerker, Tommy A. A. Karlsen, Maria Stensland, Tuula A. A. Nyman, Simon Rayner, Jan E. E. Brinchmann
Summary: Focal lesions of articular cartilage can cause pain and reduced joint function, leading to osteoarthritis if untreated. In vitro generated, scaffold-free autologous cartilage discs made from articular chondrocytes were compared to those made from bone marrow-derived mesenchymal stromal cells. Articular chondrocytes produced more extracellular matrix and contained more articular cartilage proteins, while mesenchymal stromal cells had more proteins associated with cartilage hypertrophy and bone formation. MicroRNA analysis suggested that differential expression of microRNAs played a key role in the differential synthesis of proteins. Therefore, articular chondrocytes are preferred for engineering articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Andres Pazos-Perez, Maria Pineiro-Ramil, Eloi Franco-Trepat, Maria Guillan-Fresco, Veronica Lopez-Lopez, Alberto Jorge-Mora, Ana Alonso-Perez, Rodolfo Gomez
Summary: The study found that methylphenidate hydrochloride (MPH) may have an impact on the growth plate, leading to premature closure and growth retardation in patients with attention deficit hyperactivity disorder treated with MPH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Jonathan C. Bernhard, Darja Marolt Presen, Ming Li, Xavier Monforte, James Ferguson, Gabriele Leinfellner, Patrick Heimel, Susanna L. Betti, Sharon Shu, Andreas H. Teuschl-Woller, Stefan Tangl, Heinz Redl, Gordana Vunjak-Novakovic
Summary: In this study, the effects of endochondral and intramembranous ossification pathways on the properties of engineered bone grafts were evaluated. The results showed that grafts generated through endochondral pathway exhibited better bone regeneration and vascularization, making it more suitable for regenerating large defects. On the other hand, the intramembranous ossification pathway could be used to guide tissue formation with scaffold architecture.
Article
Engineering, Biomedical
Gabriella Nilsson Hall, Amit Chandrakar, Angela Pastore, Konstantinos Ioannidis, Katrina Moisley, Matei Cirstea, Liesbet Geris, Lorenzo Moroni, Frank P. Luyten, Paul Wieringa, Ioannis Papantoniou
Summary: Bone fractures can be slow to heal or not heal at all in 2-12% of cases. Autologous grafts are currently used but have limitations. Tissue engineered cell-based implants have potential as an alternative treatment. In this study, cartilaginous spheroids were combined with melt electrowritten (MEW) meshes to create an engineered cell-based implant capable of inducing bone formation.
ACTA BIOMATERIALIA
(2023)
Review
Cell Biology
Chee Ho H'ng, Ashwini Khaladkar, Alberto Rosello-Diez
Summary: The balance of cell proliferation and size is crucial for organ development and repair. The mechanistic target of rapamycin (mTOR) signaling pathway, which interacts with other pathways and influences cell proliferation, size, and differentiation, plays a critical role in coordinating the complex developmental transitions during limb development. Therefore, studying the interaction of mTOR components with signaling pathways and transcription factors involved in limb patterning, morphogenesis, and growth could enhance our understanding of these processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Ying-Jie Guan, Jing Li, Xu Yang, Shaohua Du, Jing Ding, Yun Gao, Ying Zhang, Kun Yang, Qian Chen
Article
Cell Biology
Huixia Li, Danhui Li, Zhengmin Ma, Zhuang Qian, Xiaomin Kang, Xinxin Jin, Fang Li, Xinluan Wang, Qian Chen, Hongzhi Sun, Shufang Wu
Article
Multidisciplinary Sciences
Chathuraka T. Jayasuriya, Nan Hu, Jing Li, Nicholas Lemme, Richard Terek, Michael G. Ehrlich, Qian Chen
SCIENTIFIC REPORTS
(2018)
Article
Chemistry, Multidisciplinary
Zhongning Liu, Xin Chen, Zhanpeng Zhang, Xiaojin Zhang, Laura Saunders, Yongsheng Zhou, Peter X. Ma
Review
Biochemistry & Molecular Biology
Laura Saunders, Peter X. Ma
MACROMOLECULAR BIOSCIENCE
(2019)
Article
Anatomy & Morphology
Qi-ling Yuan, Yin-gang Zhang, Qian Chen
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
John Twomey-Kozak, Salomi Desai, Wenguang Liu, Neill Y. Li, Nicholas Lemme, Qian Chen, Brett D. Owens, Chathuraka T. Jayasuriya
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Cell Biology
Yajun Liu, Jonah Schwam, Qian Chen
Summary: Aging is a process of functional decline and tissue degeneration that occurs as organisms age. Cellular senescence, caused by replicative stress, telomere erosion, and stem cell exhaustion, plays a key role in tissue degradation and cell death in aging-related diseases. The interaction between senescent and non-senescent cells may lead to the death of non-senescent cells and tissue degeneration in aging organs.
Review
Chemistry, Medicinal
Brandon Vorrius, Zhen Qiao, Jonathan Ge, Qian Chen
Summary: The musculoskeletal system is composed of specialized connective tissues that provide protection, structure, and mobility to the body. However, diseases of the musculoskeletal system are increasing in burden, resulting in pain, disability, and a decrease in quality of life. Regenerative drug therapies for musculoskeletal disorders are in demand, but effective drug delivery poses challenges.
Article
Mathematics, Interdisciplinary Applications
Zhen Qiao, Hongtao Zhang, Hai-Feng Ji, Qian Chen
Article
Rheumatology
Nan Hu, Yun Gao, Chathuraka T. Jayasuriya, Wenguang Liu, Heng Du, Jing Ding, Meng Feng, Qian Chen
ARTHRITIS RESEARCH & THERAPY
(2019)
Review
Cell & Tissue Engineering
Ming Dang, Laura Saunders, Xufeng Niu, Yubo Fan, Peter X. Ma
Article
Cell Biology
Longhuo Wu, Haiqing Liu, Linfu Li, Daohua Xu, Yun Gao, Yingjie Guan, Qian Chen
CONNECTIVE TISSUE RESEARCH
(2018)