Article
Dentistry, Oral Surgery & Medicine
Cong Shi, Liting Cai, Zhe Xun, Saiwei Zheng, Fangyang Shao, Bingjie Wang, Ruixin Zhu, Yuan He
Summary: This study revealed significant differences in salivary bacterial community among individuals with caries, periodontitis, and comorbid diseases, with periodontitis patients showing increased complexity in their salivary microbiota. The analysis identified potential biomarkers and intricate bacterial interactions among the groups. Additionally, predicted functions related to caries and periodontitis, such as carbohydrate metabolism and bacteria proliferation, were highlighted.
JOURNAL OF DENTAL SCIENCES
(2021)
Article
Immunology
Boyang Sun, Bingyao Liu, Xiaojiao Gao, Kai Xing, Li Xie, Ting Guo
Summary: Salivary microbial communities differ significantly among patients with Crohn's disease-associated periodontitis, patients with periodontitis, and healthy individuals, with distinct microbial abundances and gene functions observed. Most antibiotic resistance genes were found in the Pm group, indicating potential implications for clinical diagnosis and treatment of oral diseases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Miguel Carda-Dieguez, Rebecca Moazzez, Alex Mira
Summary: In this study, genomic and transcriptomic analyses of human dental plaque showed that long-term use of fluoride-containing toothpaste and fluoride+arginine toothpaste can change the composition and functional profiles of oral microbial communities, promoting a healthier microbial community.
Review
Microbiology
Jessica Alves Cena, Leticia Goncalves Reis, Ana Karolina Almeida de Lima, Camilla Pedrosa Vieira Lima, Cristine Miron Stefani, Naile Dame-Teixeira
Summary: This systematic review compared the salivary microbiota of adults with type 2 diabetes mellitus (T2D) to those without T2D, focusing on acid-associated bacteria. It found that diabetics had a higher abundance of acidogenic and aciduric bacteria, as well as enrichment of specific acid-associated genera.
Article
Medicine, Legal
Feyza Guel, Sukriye Karadayi, Zuhal Yurdabakan, Tuelin Oezbek, Beytullah Karadayi
Summary: This study analyzed the changes in saliva microbiota composition in individuals with active dental caries before and after treatment using sequencing methods. The results showed that while the microbiota composition remained relatively stable after treatment, there were significant differences in the relative abundance of certain bacterial genera and species.
FORENSIC SCIENCE INTERNATIONAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
R. Raksakmanut, P. Thanyasrisung, S. Sritangsirikul, K. Kitsahawong, A. L. Seminario, W. Pitiphat, O. Matangkasombut
Summary: Dental caries, the most common chronic disease in children, has negative effects on their health, development, and well-being. Early preventive interventions are crucial, but there is still a lack of accurate caries risk predictors for infants before caries onset.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Lais Daniela Ev, Joice de Faria Poloni, Naile Dame-Teixeira, Rodrigo Alex Arthur, Daniela Jorge Corralo, Sandra Liana Henz, Thuy Do, Marisa Maltz, Clarissa Cavalcanti Fatturi Parolo
Summary: This study evaluated the microbial functional profile of biofilms related to different clinical conditions related to caries or health. Metatranscriptomic analysis identified specific genes and gene modules strongly related to caries status, and gene ontology analysis revealed important bioprocesses related to caries-free conditions.
CLINICAL ORAL INVESTIGATIONS
(2023)
Review
Endocrinology & Metabolism
G. Dipalma, A. D. Inchingolo, F. Inchingolo, I. A. Charitos, M. Di Cosola, A. P. Cazzolla
Summary: This paper discusses the biochemical interactions between teeth and the oral environment during the caries process, including physico-chemical reactions, theoretical basis for prevention and treatment, 'demineralization' and 'remineralization', the role of microbiota, as well as the mechanisms of carious lesion development, healing, and repair.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2021)
Review
Microbiology
Yi Huang, Xinyuan Zhao, Li Cui, Shaohong Huang
Summary: The oral microbiome is closely related to oral and systemic health, with dental plaque biofilms being the primary factor of periodontitis. Analyzing the composition and functional characteristics of microbial communities is crucial for understanding the pathogenesis of periodontitis.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Immunology
Yuwei Zhang, Liang Ren, Qi Wang, Zhining Wen, Chengcheng Liu, Yi Ding
Summary: Oral diseases have a significant impact on global health, and are related to oral microbes. Effective measures for prevention and promotion of oral health are needed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Neurosciences
Yi Yang, Jiaxi Lv, Huimin Bai, Liang Ren, Jing Yang, Yi Ding, Chengcheng Liu, Xueping Chen
Summary: Characterizing the periodontal status of patients with Alzheimer's disease (AD), investigating differences in salivary metabolism between patients with and without AD under the same periodontal conditions, and understanding how it is related to oral flora are critical. Dysregulation of specific bacterial flora in saliva plays a vital role in metabolic changes in AD.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Craig S. Miller, Robert J. Danaher, Sree Kirakodu, Charles R. Carlson, Russell J. Mumper
Summary: Chewing gum containing freeze-dried blackberry powder can significantly reduce the quantity of various oral bacteria in saliva, including major oral bacterial strains. Regardless of the CG or BG group, S. sanguinis and A. naeslundii were the predominant bacteria adherent to enamel after gum chewing, with S. mutans representing only a small percentage of the total bacteria on enamel.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Engineering, Environmental
Xiaojian Wu, Zongbao Liu, Meng Li, Mark Bartlam, Yingying Wang
Summary: In this study, the occurrence, abundance, and transcriptional level of antibiotic resistance genes (ARGs), plasmid associated ARGs, microbial composition, and ARG bacterial hosts were analyzed in the plastisphere and urban river water. The results showed that the plastisphere is a hotspot for acquisition, expression, and dissemination of antibiotic resistance genes.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Yago Gecy de Sousa Ne, Weslley Ferreira Lima, Paulo Fernando Santos Mendes, Daiane Claydes Baia-da-Silva, Leonardo Oliveira Bittencourt, Priscila Cunha Nascimento, Renata Duarte de Souza-Rodrigues, Luiz Renato Paranhos, Paulo Antonio Martins-Junior, Rafael Rodrigues Lima
Summary: This study aimed to analyze the research trends on salivary oxidative stress associated with dental caries and to perform bibliometric approaches for existing publications on this association. The findings show that India and Asia have the highest number of publications on this topic, non-stimulated total saliva is the most commonly collected sample, and total antioxidant capacity is the most frequently evaluated parameter. Cross-sectional studies are the most common study design, and articles published from 2017 to 2022 are the most frequent.
Review
Immunology
Hao Dong, Jiaxin Liu, Jianhui Zhu, Zhiyan Zhou, Marco Tizzano, Xian Peng, Xuedong Zhou, Xin Xu, Xin Zheng
Summary: Taste receptors play a role in the interaction between oral microbiota and the host, affecting the occurrence and development of oral diseases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Thomas Flagstad, Mette T. Pedersen, Tim H. Jakobsen, Jakob Felding, Tim Tolker-Nielsen, Michael Givskov, Katrine Qvortrup, Thomas E. Nielsen
Summary: There is a growing global demand for new and more effective antibiotics to combat multi-resistant bacteria. This has resulted in extensive research on novel bacterial topoisomerase inhibitors (NBTIs) that target key bacterial enzymes DNA gyrase and topoisomerase IV. However, some NBTI compounds have been found to inhibit the hERG potassium channel, causing undesired cardiac arrhythmia and complicating medicinal chemistry efforts. In this study, a solid-phase strategy is presented for the rapid synthesis of a promising new class of NBTIs.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Jiuxin Qu, Zhao Cai, Xiangke Duan, Han Zhang, Hang Cheng, Shuhong Han, Kaiwei Yu, Zhaofang Jiang, Yingdan Zhang, Yang Liu, Fang Bai, Yingxia Liu, Lei Liu, Liang Yang
Summary: Our study suggests that SARS-CoV-2 infection can lead to genetic adaptation of bacterial pathogens, promoting chronic infections. The adapted bacteria exhibit characteristics such as excessive biofilm formation and reduced virulence, enabling them to evade immune clearance. This highlights the importance of understanding the lung microenvironment post-virus infections and superinfection mechanism in COVID-19 patients.
CELL AND BIOSCIENCE
(2022)
Article
Chemistry, Medicinal
Jing Wang, Xi Liu, Kaiwei Yu, Moxiao Liu, Jiuxin Qu, Yingxia Liu, Zhao Cai, Ke Wang, Chao Zhuo, Liang Yang, Yingdan Zhang
Summary: The co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) in ICU patients is common but challenging to eliminate. Research suggests that antibiotic exposure reshapes the community compositions of dual-species biofilms, with different EPS structures playing roles in maintaining stability, highlighting the potential of targeting EPS structural fibers like Psi and extracellular DNA (eDNA) as a strategy for controlling polymicrobial biofilm-related infections.
ACS INFECTIOUS DISEASES
(2022)
Review
Biotechnology & Applied Microbiology
Xiaobo Liu, Bin Cao, Liang Yang, Ji-Dong Gu
Summary: Biofilm formation and biofilm-induced biodeterioration have significant impacts on the community. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a key signaling molecule in bacteria, regulating various bacterial processes. Understanding the regulatory mechanisms of c-di-GMP would benefit the control of bacterial processes and the development of anti-biofilm technologies.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Yu-Ming Cai, Kai-Wei Yu, Ji-Hong Liu, Zhao Cai, Zun-Hao Zhou, Yang Liu, Tian-Fu Wang, Liang Yang
Summary: The c-di-GMP signaling pathways in P. aeruginosa are highly organized and play important roles in the transition between motile and sessile lifestyles. This study identified a phosphodiesterase, PipA, that regulates bacteriophage production, and uncovered a potential signaling circuit for biofilm treatment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Tianyuan Jia, Dongjing Liu, Xianbiao Bi, Menglu Li, Zhao Cai, Jiapeng Fu, Zhi Liu, Pengyao Wu, Xue Ke, Aiqun Jia, Guoliang Zhang, Guobao Li, Liang Yang
Summary: This study reveals that the AhR ligand Pht and its vitamin K analogs can inhibit the quorum sensing mechanism of P. aeruginosa and activate immune-modulating functions, suggesting their potential as alternative treatments for infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yu Fen Goh, Henriette L. L. Roder, Siew Herng Chan, Muhammad Hafiz Ismail, Jonas S. S. Madsen, Kai Wei Kelvin Lee, Soren J. Sorensen, Michael Givskov, Mette Burmolle, Scott A. A. Rice, Diane McDougald
Summary: Mixed species biofilms exhibit increased tolerance to stresses. Grazing by Tetrahymena pyriformis significantly reduced the biofilms of single species K. pneumoniae and P. protegens, but P. aeruginosa biofilms were resistant. Rhamnolipids and the quorum sensing molecule PQS contribute to the protective mechanisms.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Joey Kuok Hoong Yam, Leon Zhen Wei Tan, Ziyan Hong, May Margarette Santillan Salido, Bau Yi Woo, Adeline Mei Hui Yong, Casandra Ai Zhu Tan, Sam Fong Yau Li, Liang Yang, Michael Givskov, Shu-Sin Chng
Summary: Pseudomonas aeruginosa is a major cause of hospital-acquired infections. This study discovered that the FDA-approved drug auranofin can attenuate virulence pathways in P. aeruginosa, including quorum sensing and Type IV pili. Auranofin acts through multiple targets, one of which is Vfr. It reduces biofilm maturation and exhibits strong synergy with colistin in eradicating P. aeruginosa biofilms.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Food Science & Technology
Ge Zhao, Paul Joseph Kempen, Tao Zheng, Tim Holm Jakobsen, Shuangqing Zhao, Liuyan Gu, Christian Solem, Peter Ruhdal Jensen
Summary: In this study, the synergistic antibacterial activity of nisin and phytic acid against foodborne E. coli strains was evaluated. The results showed a synergistic effect on certain strains and an addictive effect on others. The combined bactericidal effect of nisin and phytic acid was also investigated on E. coli O157:H7, and significant effects on cell numbers and morphology were observed.
Article
Microbiology
Christina Manner, Raphael Dias Teixeira, Dibya Saha, Andreas Kaczmarczyk, Raphaela Zemp, Fabian Wyss, Tina Jaeger, Benoit-Joseph Laventie, Sebastien Boyer, Jacob G. Malone, Katrine Qvortrup, Jens Bo Andersen, Michael Givskov, Tim Tolker-Nielsen, Sebastian Hiller, Knut Drescher, Urs Jenal
Summary: HecRE is a molecular switch that regulates cyclic di-GMP levels and promotes binary cell states during Pseudomonas aeruginosa surface colonization and biofilm development. This finding provides a new approach to control surface infections.
NATURE MICROBIOLOGY
(2023)
Article
Immunology
Mads Lichtenberg, Klaus Kirketerp-Moller, Lasse A. Kvich, Mads Holm Christensen, Blaine Fritz, Tim Holm Jakobsen, Thomas Bjarnsholt
Summary: Chronic wounds and ulcers are a growing problem with significant impact on healthcare and patients. The presence of biofilms has been identified as a contributing factor to the failure of chronic wounds to heal. Recent research has challenged the idea that biofilms are exclusive to chronic infections by discovering their presence in acute infections as well.
Article
Microbiology
Wooi Keong Teh, Yichen Ding, Francesca Gubellini, Alain Filloux, Claire Poyart, Michael Givskov, Shaynoor Dramsi
Summary: The study identified a strong association between colorectal cancer and Streptococcus gallolyticus subsp. gallolyticus (SGG). It reported the secretion of six T7SSb effector proteins by SGG strain UCN34, with one toxin named TelE exhibiting toxicity against nonimmune bacteria. This study expands the range of effector proteins secreted through T7SSb.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Mads Lichtenberg, Tom Coenye, Matthew R. Parsek, Thomas Bjarnsholt, Tim Holm Jakobsen
Summary: In vitro biofilms are distinct microbial communities characterized by physical traits, phenotypic features, and antibiotic tolerance. The importance of these traits varies depending on the environment and bacterial species. Mechanisms enabling antibiotic resistance in biofilms include physical barriers, physiological adaptations, and changes in gene expression. However, there is little consensus on gene expression profiles in biofilms, and a definitive "biofilm signature transcriptome" has not been identified. Spatial and temporal variability in biofilms greatly differs across systems or environments. Clinical biofilms may differ from those cultivated in laboratories and found in the environment, emphasizing the need for a better understanding of their characteristics.
FEMS MICROBIOLOGY REVIEWS
(2023)
Review
Microbiology
Mads Lichtenberg, Tim Holm Jakobsen, Michael Kuhl, Mette Kolpen, Peter Ostrup Jensen, Thomas Bjarnsholt
Summary: Pseudomonas aeruginosa establishes a microenvironmental niche in chronic infections, adapting to host responses by slowing down metabolism and exhibiting distinct adaptive traits. Understanding the microenvironmental ecology of P. aeruginosa in chronic infections is crucial for combating persistent bacterial infections.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Microbiology
Guohui Xiao, Zhao Cai, Qinglong Guo, Taosheng Ye, Yimin Tang, Peikun Guan, Juanjuan Zhang, Min Ou, Xiangdong Fu, Lili Ren, Minfei Yu, Zhaoqin Wang, Lei Liu, Liang Yang, Guoliang Zhang
Summary: This study aimed to determine the lung microbiota profile associated with pulmonary tuberculosis (TB) and characterize the changes during anti-TB treatment. The lung microbiota of untreated pulmonary TB patients was found to be distinct from that of healthy individuals and lung cancer patients. Anti-TB treatment significantly affected the diversity of the lung microbiota and induced the presence of antibiotic resistance genes.
MICROBIOLOGY SPECTRUM
(2022)